In vitro differentiation of transplantable neural precursors from human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-12

AUTHORS

S C Zhang, M Wernig, I D Duncan, O Brüstle, J A Thomson

ABSTRACT

The remarkable developmental potential and replicative capacity of human embryonic stem (ES) cells promise an almost unlimited supply of specific cell types for transplantation therapies. Here we describe the in vitro differentiation, enrichment, and transplantation of neural precursor cells from human ES cells. Upon aggregation to embryoid bodies, differentiating ES cells formed large numbers of neural tube-like structures in the presence of fibroblast growth factor 2 (FGF-2). Neural precursors within these formations were isolated by selective enzymatic digestion and further purified on the basis of differential adhesion. Following withdrawal of FGF-2, they differentiated into neurons, astrocytes, and oligodendrocytes. After transplantation into the neonatal mouse brain, human ES cell-derived neural precursors were incorporated into a variety of brain regions, where they differentiated into both neurons and astrocytes. No teratoma formation was observed in the transplant recipients. These results depict human ES cells as a source of transplantable neural precursors for possible nervous system repair. More... »

PAGES

1129-1133

Journal

TITLE

Nature Biotechnology

ISSUE

12

VOLUME

19

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1201-1129

    DOI

    http://dx.doi.org/10.1038/nbt1201-1129

    DIMENSIONS

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    PUBMED

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