Optimization of humanized IgGs in glycoengineered Pichia pastoris View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-02

AUTHORS

Huijuan Li, Natarajan Sethuraman, Terrance A Stadheim, Dongxing Zha, Bianka Prinz, Nicole Ballew, Piotr Bobrowicz, Byung-Kwon Choi, W James Cook, Michael Cukan, Nga Rewa Houston-Cummings, Robert Davidson, Bing Gong, Stephen R Hamilton, Jack P Hoopes, Youwei Jiang, Nam Kim, Renee Mansfield, Juergen H Nett, Sandra Rios, Rendall Strawbridge, Stefan Wildt, Tillman U Gerngross

ABSTRACT

As the fastest growing class of therapeutic proteins, monoclonal antibodies (mAbs) represent a major potential drug class. Human antibodies are glycosylated in their native state and all clinically approved mAbs are produced by mammalian cell lines, which secrete mAbs with glycosylation structures that are similar, but not identical, to their human counterparts. Glycosylation of mAbs influences their interaction with immune effector cells that kill antibody-targeted cells. Here we demonstrate that human antibodies with specific human N-glycan structures can be produced in glycoengineered lines of the yeast Pichia pastoris and that antibody-mediated effector functions can be optimized by generating specific glycoforms. Glycoengineered P. pastoris provides a general platform for producing recombinant antibodies with human N-glycosylation. More... »

PAGES

210-215

Journal

TITLE

Nature Biotechnology

ISSUE

2

VOLUME

24

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1178

    DOI

    http://dx.doi.org/10.1038/nbt1178

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030021520

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16429149


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    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1178'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1178'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1178'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1178'


     

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