Derivation of human embryonic stem cells in defined conditions View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-02

AUTHORS

Tenneille E Ludwig, Mark E Levenstein, Jeffrey M Jones, W Travis Berggren, Erika R Mitchen, Jennifer L Frane, Leann J Crandall, Christine A Daigh, Kevin R Conard, Marian S Piekarczyk, Rachel A Llanas, James A Thomson

ABSTRACT

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions. More... »

PAGES

185-187

Journal

TITLE

Nature Biotechnology

ISSUE

2

VOLUME

24

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1177

    DOI

    http://dx.doi.org/10.1038/nbt1177

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030407065

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16388305


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