Derivation of human embryonic stem cells in defined conditions View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-02

AUTHORS

Tenneille E Ludwig, Mark E Levenstein, Jeffrey M Jones, W Travis Berggren, Erika R Mitchen, Jennifer L Frane, Leann J Crandall, Christine A Daigh, Kevin R Conard, Marian S Piekarczyk, Rachel A Llanas, James A Thomson

ABSTRACT

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions. More... »

PAGES

185-187

Journal

TITLE

Nature Biotechnology

ISSUE

2

VOLUME

24

Author Affiliations

Related Patents

  • Human Cancer Stem Cell Culture Compositions Comprising Erbb2 Variants And Methods Of Use Thereof
  • Recombinant Laminin-521
  • Cell Culture Medium
  • Induced Pluripotent Stem Cells Produced With Oct3/4, Klf4 And Sox2
  • Cells Expressing Pluripotency Markers And Expressing Markers Characteristic Of The Definitive Endoderm
  • Stem Cell Defined Media For Xeno-Free And Feeder Free Conditions And Uses Thereof
  • Stem Cell Cultures
  • Fgf Having Enhanced Stability
  • Human Embryonic Stem Cells Genetically Modified To Contain A Nucleic Acid And Cultured With Fibroblast Growth Factor
  • Stem Cell Cultures
  • Methods And Products For Transfection
  • Simplified Basic Media For Human Pluripotent Cell Culture
  • Method For Production Of Mast Cells From Stem Cells
  • (Meth)Acrylate Surfaces For Cell Culture, Methods Of Making And Using The Surfaces
  • Methods And Compositions For Growth Of Embryonic Stem Cells
  • Methods And Products For Transfecting Cells
  • Early Mesoderm Cells, A Stable Population Of Mesendoderm Cells That Has Utility For Generation Of Endoderm And Mesoderm Lineages And Multipotent Migratory Cells (Mmc)
  • Defined Media For Expansion And Maintenance Of Pluripotent Stem Cells
  • Composition And Method For Enabling Proliferation Of Pluripotent Human Stem Cells
  • Methods And Products For Expressing Proteins In Cells
  • Methods And Systems For Converting Precursor Cells Into Intestinal Tissues Through Directed Differentiation
  • Thermostable Fgf-2 Mutant Having Enhanced Stability
  • Trophoblast-Derived Mesenchymal Stem Cells (T-Mscs) Produced From Human Embryonic Stem Cells, Methods And Uses Thereof
  • Methods Of Enhancing Cell Survival Of Stem Cells
  • Suspension Culture Of Human Embryonic Stem Cells
  • Composition And Method For Enabling Proliferation Of Pluripotent Stem Cells
  • Primate Pluripotent Stem Cell Expansion Without Feeder Cells And In The Presence Of Fgf And Matrigel Or Engelbreth-Holm-Swarm Tumor Cell Preparation
  • Hla G-Modified Cells And Methods
  • Preparing Tooth-Like Structure Using Stem Cell
  • Isolated Naive Pluripotent Stem Cells And Methods Of Generating Same
  • Substrates And Methods For Culturing Stem Cells
  • Cell Culture Substrate Comprising A Laminin And A Cadherin
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Methods And Products For Expressing Proteins In Cells
  • Methods And Products For Expressing Proteins In Cells
  • Stem Cell Defined Media For Xeno-Free And Feeder Free Conditions And Uses Thereof
  • Mesenchymal-Like Stem Cells Derived From Human Embryonic Stem Cells, Methods And Uses Thereof
  • Swellable (Meth)Acrylate Surfaces For Culturing Cells In Chemically Defined Media
  • Method To Perform Beam-Type Collision-Activated Dissociation In The Pre-Existing Ion Injection Pathway Of A Mass Spectrometer
  • A Method For Obtaining A Xeno-Free Hbs Cell Line
  • Medium For Growing Human Embryonic Stem Cells
  • Cell Culture Composition Comprising Laminin-521
  • Method To Perform Beam-Type Collision-Activated Dissociation In The Pre-Existing Ion Injection Pathway Of A Mass Spectrometer
  • Generation Of Induced Pluripotent Stem Cells From Small Volumes Of Peripheral Blood
  • Somatic Cell Reprogramming By Retroviral Vectors Encoding Oct3/4. Klf4, C-Myc And Sox2
  • Culture Medium Containing Gamma-Aminobutyric Acid, Pipecolic Acid Or Lithium For The Maintenance Of Stem Cells In An Undifferentiated State
  • Method For Extracellular Matrix Mediated Differentiation And Proliferation Of Stem Cells
  • Simplified Basic Media For Human Pluripotent Cell Culture
  • Methods And Products For Transfecting Cells
  • Synthetic Surfaces For Culturing Stem Cell Derived Oligodendrocyte Progenitor Cells
  • Methods And Products For Nucleic Acid Production And Delivery
  • Methods And Platforms For Drug Discovery Using Induced Pluripotent Stem Cells
  • Methods For Producing Enucleated Erythroid Cells Derived From Pluripotent Stem Cells
  • Methods For The Production Of Ips Cells Using Epstein-Barr (Ebv)-Based Reprogramming Vectors
  • Scalable Primate Pluripotent Stem Cell Aggregate Suspension Culture And Differentiation Thereof
  • Priming Of Pluripotent Stem Cells For Neural Differentiation
  • Automated Method And Apparatus For Embryonic Stem Cell Culture
  • Method To Perform Beam-Type Collision-Activated Dissociation In The Pre-Existing Ion Injection Pathway Of A Mass Spectrometer
  • Generation Of Induced Pluripotent Stem Cells From Small Volumes Of Peripheral Blood
  • Methods And Materials For Obtaining Induced Pluripotent Stem Cells
  • Methods And Products For Expressing Proteins In Cells
  • Methods Of Expanding Embryonic Stem Cells In A Suspension Culture
  • Engineering A Heterogeneous Tissue From Pluripotent Stem Cells
  • Chitosan-Alginate Scaffold Cell Culture System And Related Methods
  • Screening Methods For Human Embryonic Stem Cells
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Primate Pluripotent Stem Cells Cultured In Medium Containing Gamma-Aminobutyric Acid, Pipecolic Acid And Lithium
  • Defined Conditions For Human Embryonic Stem Cell Culture And Passage
  • Human Blood-Brain Endothelial Cells Derived From Pluripotent Stem Cells And Blood-Brain Barrier Model Thereof
  • Ecm Composition, Tumor Microenvironment Platform And Methods Thereof
  • Methods Of Enhancing Genetic Diagnosis
  • Growth Of Cells
  • Generating Pluripotent Cells De Novo
  • Differentiation Of Pluripotent Cells
  • Fgf Having Enhanced Stability
  • Methods And Products For Transfection
  • Simplified Basic Media For Human Pluripotent Cell Culture
  • Preparing Tooth-Like Structure Using Stem Cell
  • Methods And Compositions For Growth Of Cells And Embryonic Tissue On A Synthetic Polymer Matrix
  • Differentiation Of Pluripotent Cells Into Primary Germ Layer Progenitors
  • Method For Production Of Mast Cells From Stem Cells
  • Stem Cell Culture Method
  • Methods And Materials For Obtaining Induced Pluripotent Stem Cells
  • Methods Of Producing Rpe Cells
  • Providing Ipscs To A Customer
  • Methods For The Production Of Ips Cells Using Non-Viral Approach
  • Reprogramming Immortalized B-Cells To Induced Pluripotent Stem Cells
  • Differentiation Of Human Pluripotent Stem Cells Into Cells Expressing Mature Hepatocyte Markers By 2d And Bioreactor Culture
  • Methods And Products For Transfection
  • Synthetic Surfaces For Culturing Cells In Chemically Defined Media
  • Priming Of Pluripotent Stem Cells For Neural Differentiation
  • Cells Expressing Pluripotency Markers And Expressing Markers Characteristic Of The Definitive Endoderm
  • Nuclear Reprogramming Factor
  • Composition And Method For Enabling Proliferation Of Pluripotent Human Stem Cells
  • 3d Culturing Systems For Growth And Differentiation Of Human Pluripotent Stem (Hps) Cells
  • Methods And Compositions For The Differentiation Of Stem Cells
  • Undifferentiated Stem Cell Culture Systems
  • Medium For Growing Human Embryonic Stem Cells
  • Pluripotent Stem Cell-Derived 3d Retinal Tissue And Uses Thereof
  • Synthetic Surfaces For Culturing Stem Cell Derived Cardiomyocytes
  • Methods And Products For Expressing Proteins In Cells
  • Reprogramming Somatic Cells Using Retroviral Vectors Comprising Oct-4 And Sox2 Genes
  • A Method For Carrying Out Of The Beam The Form Of Shock Activated Dissociation In The Already Existing Ions Injection Path Of A Mass Spectrometer
  • Methods And Products For Transfecting Cells
  • Methods For The Production Of Ips Cells
  • Cell Culture Article And Screening
  • Pericyte Progenitors From Peripheral Blood
  • Methods And Products For Expressing Proteins In Cells
  • Three-Dimensional Cell Culturing
  • Cell Culture Substrate Comprising A Laminin And A Cadherin
  • Oct3/4, Klf4, C-Myc And Sox2 Produce Induced Pluripotent Stem Cells
  • Synthetic Surfaces For Culturing Stem Cell Derived Cardiomyocytes
  • Stem Cell Cultures
  • Reprogramming Cells To A Less Differentiated State
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • Methods Of Reprogramming Cells To A Less Differentiated State
  • Composition And Method For Enabling Proliferation Of Pluripotent Stem Cells
  • Products For Transfection And Reprogramming
  • Micro-Carrier Culture System For Rapid Expansion Of Human Embryonic Stem Cells
  • Novel Methods And Culture Media For Culturing Pluripotent Stem Cells
  • Methods And Products For Reprogramming Cells To A Less Differentiated State
  • Cell Culture Substrate
  • Media For Culturing Stem Cells
  • Mesenchymal-Like Stem Cells Derived From Human Embryonic Stem Cells, Methods And Uses Thereof
  • Synthetic Surfaces For Culturing Stem Cell Derived Cardiomyocytes
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1177

    DOI

    http://dx.doi.org/10.1038/nbt1177

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030407065

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16388305


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Proliferation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Survival", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tissue Engineering", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "WiCell", 
              "id": "https://www.grid.ac/institutes/grid.439113.d", 
              "name": [
                "WiCell Research Institute, PO Box 7365, Madison, Wisconsin 53707-7365, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ludwig", 
            "givenName": "Tenneille E", 
            "id": "sg:person.01154664726.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154664726.94"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Levenstein", 
            "givenName": "Mark E", 
            "id": "sg:person.01145062633.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145062633.75"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Jones", 
            "givenName": "Jeffrey M", 
            "id": "sg:person.0721160752.90", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721160752.90"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Berggren", 
            "givenName": "W Travis", 
            "id": "sg:person.0735657217.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735657217.13"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Mitchen", 
            "givenName": "Erika R", 
            "type": "Person"
          }, 
          {
            "familyName": "Frane", 
            "givenName": "Jennifer L", 
            "id": "sg:person.01050140115.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050140115.64"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Crandall", 
            "givenName": "Leann J", 
            "type": "Person"
          }, 
          {
            "familyName": "Daigh", 
            "givenName": "Christine A", 
            "id": "sg:person.01164366515.87", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164366515.87"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Conard", 
            "givenName": "Kevin R", 
            "id": "sg:person.01327424433.69", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327424433.69"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Piekarczyk", 
            "givenName": "Marian S", 
            "id": "sg:person.0673607765.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673607765.00"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Llanas", 
            "givenName": "Rachel A", 
            "id": "sg:person.01346730315.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346730315.40"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Thomson", 
            "givenName": "James A", 
            "id": "sg:person.012266000337.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012266000337.10"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nm979", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000301825", 
              "https://doi.org/10.1038/nm979"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm979", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000301825", 
              "https://doi.org/10.1038/nm979"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0028-3908(86)90263-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002391209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0028-3908(86)90263-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002391209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt922", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005956847", 
              "https://doi.org/10.1038/nbt922"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt922", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005956847", 
              "https://doi.org/10.1038/nbt922"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1095/biolreprod.103.021147", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007519570"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.2235735100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014717888"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.41.7.432", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016425813"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0140-6736(05)66473-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019112974"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt761", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021609375", 
              "https://doi.org/10.1038/nbt761"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt761", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021609375", 
              "https://doi.org/10.1038/nbt761"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.282.5391.1145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028898955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029326356", 
              "https://doi.org/10.1038/nmeth744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029326356", 
              "https://doi.org/10.1038/nmeth744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(03)00847-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031003004"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1181", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033356897", 
              "https://doi.org/10.1038/nm1181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1181", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033356897", 
              "https://doi.org/10.1038/nm1181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0074-7696(02)13011-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040376015"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2004-0211", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045135667"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/dbio.2000.9912", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053693663"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2006-02", 
        "datePublishedReg": "2006-02-01", 
        "description": "We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt1177", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2622167", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "24"
          }
        ], 
        "name": "Derivation of human embryonic stem cells in defined conditions", 
        "pagination": "185-187", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "90db21e3da9b511c6ea4283692500f04cccc47c4dd2488c9d8c64ce5e555a3b5"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "16388305"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1177"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1030407065"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1177", 
          "https://app.dimensions.ai/details/publication/pub.1030407065"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:15", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_54014_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/nbt/journal/v24/n2/full/nbt1177.html"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1177'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1177'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1177'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1177'


     

    This table displays all metadata directly associated to this object as RDF triples.

    221 TRIPLES      21 PREDICATES      53 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1177 schema:about N258c2340006f4baba69c0a8934657b8d
    2 N655f8fced2934a67aa52884a03e5a4e5
    3 N7a63b22f58fc4d05b44683fd58d7afe9
    4 N91ae36ac937449ec851227ae1b7210f9
    5 N9943d284bb2b4028b76e86c6481a78f2
    6 Nc6df87619d7b4813aba901e90d44389d
    7 Ne66d3cb1686c4bfa81d313f83f79f508
    8 Nec907f0aef0641838534e2b0d043e3a3
    9 Nf6de6c185ad147b281bbb6e147d7b177
    10 anzsrc-for:06
    11 anzsrc-for:0601
    12 schema:author N6ee5377a25384d75bc94e4cac7ec0790
    13 schema:citation sg:pub.10.1038/nbt761
    14 sg:pub.10.1038/nbt922
    15 sg:pub.10.1038/nm1181
    16 sg:pub.10.1038/nm979
    17 sg:pub.10.1038/nmeth744
    18 https://doi.org/10.1006/dbio.2000.9912
    19 https://doi.org/10.1016/0028-3908(86)90263-7
    20 https://doi.org/10.1016/s0074-7696(02)13011-7
    21 https://doi.org/10.1016/s0092-8674(03)00847-x
    22 https://doi.org/10.1016/s0140-6736(05)66473-2
    23 https://doi.org/10.1073/pnas.2235735100
    24 https://doi.org/10.1073/pnas.41.7.432
    25 https://doi.org/10.1095/biolreprod.103.021147
    26 https://doi.org/10.1126/science.282.5391.1145
    27 https://doi.org/10.1634/stemcells.2004-0211
    28 schema:datePublished 2006-02
    29 schema:datePublishedReg 2006-02-01
    30 schema:description We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
    31 schema:genre research_article
    32 schema:inLanguage en
    33 schema:isAccessibleForFree false
    34 schema:isPartOf Nd78f14095eec487d89372fad161a969f
    35 Nf74f0b686be4461a9224802db59cca1e
    36 sg:journal.1115214
    37 schema:name Derivation of human embryonic stem cells in defined conditions
    38 schema:pagination 185-187
    39 schema:productId N2986819e0e2b49bdb67d3c0291d57bcd
    40 N5ab4550a77b34451bce02c1f192a5b0c
    41 N6cd6ffe09ea2426daabe5c429e9c473f
    42 Ne5bd025d3d9144279a0e7147cfc79e4e
    43 Nf642096db8824b48b95a0a8c7e60ea91
    44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030407065
    45 https://doi.org/10.1038/nbt1177
    46 schema:sdDatePublished 2019-04-11T12:15
    47 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    48 schema:sdPublisher N5c7ad66c9da64761a8f322f2d5b15469
    49 schema:url http://www.nature.com/nbt/journal/v24/n2/full/nbt1177.html
    50 sgo:license sg:explorer/license/
    51 sgo:sdDataset articles
    52 rdf:type schema:ScholarlyArticle
    53 N1d2a3ef2c541419ca0242fc9323f15af schema:familyName Mitchen
    54 schema:givenName Erika R
    55 rdf:type schema:Person
    56 N1d99adcf10bd4f32a2320864937d41cd rdf:first sg:person.01164366515.87
    57 rdf:rest Ndfe680c73fe64495821d91df64b8d6d2
    58 N20d96fb2b53b497b8a5f3f4e2d44af71 rdf:first sg:person.0721160752.90
    59 rdf:rest Nced97343c5bf4dc09b82d7c38cc21831
    60 N258c2340006f4baba69c0a8934657b8d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    61 schema:name Tissue Engineering
    62 rdf:type schema:DefinedTerm
    63 N2986819e0e2b49bdb67d3c0291d57bcd schema:name dimensions_id
    64 schema:value pub.1030407065
    65 rdf:type schema:PropertyValue
    66 N2a76b20554194a58909b8d01555309d7 rdf:first sg:person.012266000337.10
    67 rdf:rest rdf:nil
    68 N5ab4550a77b34451bce02c1f192a5b0c schema:name doi
    69 schema:value 10.1038/nbt1177
    70 rdf:type schema:PropertyValue
    71 N5c7ad66c9da64761a8f322f2d5b15469 schema:name Springer Nature - SN SciGraph project
    72 rdf:type schema:Organization
    73 N655f8fced2934a67aa52884a03e5a4e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    74 schema:name Cell Culture Techniques
    75 rdf:type schema:DefinedTerm
    76 N6cd6ffe09ea2426daabe5c429e9c473f schema:name pubmed_id
    77 schema:value 16388305
    78 rdf:type schema:PropertyValue
    79 N6ee5377a25384d75bc94e4cac7ec0790 rdf:first sg:person.01154664726.94
    80 rdf:rest Nf3f7ba5bc0744c8d96ecb0aea9deb112
    81 N7a63b22f58fc4d05b44683fd58d7afe9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Humans
    83 rdf:type schema:DefinedTerm
    84 N88329777409140ecb42bde9773aba78d rdf:first sg:person.0673607765.00
    85 rdf:rest Nca28c4851e934734bdf5adf0e92ea9cd
    86 N91ae36ac937449ec851227ae1b7210f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Cell Proliferation
    88 rdf:type schema:DefinedTerm
    89 N9943d284bb2b4028b76e86c6481a78f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Cell Line
    91 rdf:type schema:DefinedTerm
    92 Na6af415eb5a0443fa9281373261112b0 rdf:first N1d2a3ef2c541419ca0242fc9323f15af
    93 rdf:rest Na84920223dd94b929ce51b4cc7c18583
    94 Na84920223dd94b929ce51b4cc7c18583 rdf:first sg:person.01050140115.64
    95 rdf:rest Nd073ae89c14e47d68d30c70c55044b1d
    96 Nc139ca408fe34a7ab85687540c68f753 schema:familyName Crandall
    97 schema:givenName Leann J
    98 rdf:type schema:Person
    99 Nc6df87619d7b4813aba901e90d44389d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Cell Differentiation
    101 rdf:type schema:DefinedTerm
    102 Nca28c4851e934734bdf5adf0e92ea9cd rdf:first sg:person.01346730315.40
    103 rdf:rest N2a76b20554194a58909b8d01555309d7
    104 Nced97343c5bf4dc09b82d7c38cc21831 rdf:first sg:person.0735657217.13
    105 rdf:rest Na6af415eb5a0443fa9281373261112b0
    106 Nd073ae89c14e47d68d30c70c55044b1d rdf:first Nc139ca408fe34a7ab85687540c68f753
    107 rdf:rest N1d99adcf10bd4f32a2320864937d41cd
    108 Nd78f14095eec487d89372fad161a969f schema:volumeNumber 24
    109 rdf:type schema:PublicationVolume
    110 Ndfe680c73fe64495821d91df64b8d6d2 rdf:first sg:person.01327424433.69
    111 rdf:rest N88329777409140ecb42bde9773aba78d
    112 Ne5bd025d3d9144279a0e7147cfc79e4e schema:name readcube_id
    113 schema:value 90db21e3da9b511c6ea4283692500f04cccc47c4dd2488c9d8c64ce5e555a3b5
    114 rdf:type schema:PropertyValue
    115 Ne66d3cb1686c4bfa81d313f83f79f508 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Stem Cells
    117 rdf:type schema:DefinedTerm
    118 Nec907f0aef0641838534e2b0d043e3a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Culture Media
    120 rdf:type schema:DefinedTerm
    121 Nf3f7ba5bc0744c8d96ecb0aea9deb112 rdf:first sg:person.01145062633.75
    122 rdf:rest N20d96fb2b53b497b8a5f3f4e2d44af71
    123 Nf642096db8824b48b95a0a8c7e60ea91 schema:name nlm_unique_id
    124 schema:value 9604648
    125 rdf:type schema:PropertyValue
    126 Nf6de6c185ad147b281bbb6e147d7b177 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Cell Survival
    128 rdf:type schema:DefinedTerm
    129 Nf74f0b686be4461a9224802db59cca1e schema:issueNumber 2
    130 rdf:type schema:PublicationIssue
    131 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    132 schema:name Biological Sciences
    133 rdf:type schema:DefinedTerm
    134 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    135 schema:name Biochemistry and Cell Biology
    136 rdf:type schema:DefinedTerm
    137 sg:grant.2622167 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt1177
    138 rdf:type schema:MonetaryGrant
    139 sg:journal.1115214 schema:issn 1087-0156
    140 1546-1696
    141 schema:name Nature Biotechnology
    142 rdf:type schema:Periodical
    143 sg:person.01050140115.64 schema:familyName Frane
    144 schema:givenName Jennifer L
    145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050140115.64
    146 rdf:type schema:Person
    147 sg:person.01145062633.75 schema:familyName Levenstein
    148 schema:givenName Mark E
    149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145062633.75
    150 rdf:type schema:Person
    151 sg:person.01154664726.94 schema:affiliation https://www.grid.ac/institutes/grid.439113.d
    152 schema:familyName Ludwig
    153 schema:givenName Tenneille E
    154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154664726.94
    155 rdf:type schema:Person
    156 sg:person.01164366515.87 schema:familyName Daigh
    157 schema:givenName Christine A
    158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164366515.87
    159 rdf:type schema:Person
    160 sg:person.012266000337.10 schema:familyName Thomson
    161 schema:givenName James A
    162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012266000337.10
    163 rdf:type schema:Person
    164 sg:person.01327424433.69 schema:familyName Conard
    165 schema:givenName Kevin R
    166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327424433.69
    167 rdf:type schema:Person
    168 sg:person.01346730315.40 schema:familyName Llanas
    169 schema:givenName Rachel A
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346730315.40
    171 rdf:type schema:Person
    172 sg:person.0673607765.00 schema:familyName Piekarczyk
    173 schema:givenName Marian S
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673607765.00
    175 rdf:type schema:Person
    176 sg:person.0721160752.90 schema:familyName Jones
    177 schema:givenName Jeffrey M
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721160752.90
    179 rdf:type schema:Person
    180 sg:person.0735657217.13 schema:familyName Berggren
    181 schema:givenName W Travis
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735657217.13
    183 rdf:type schema:Person
    184 sg:pub.10.1038/nbt761 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021609375
    185 https://doi.org/10.1038/nbt761
    186 rdf:type schema:CreativeWork
    187 sg:pub.10.1038/nbt922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005956847
    188 https://doi.org/10.1038/nbt922
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1038/nm1181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033356897
    191 https://doi.org/10.1038/nm1181
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.1038/nm979 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000301825
    194 https://doi.org/10.1038/nm979
    195 rdf:type schema:CreativeWork
    196 sg:pub.10.1038/nmeth744 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029326356
    197 https://doi.org/10.1038/nmeth744
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1006/dbio.2000.9912 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053693663
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1016/0028-3908(86)90263-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002391209
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1016/s0074-7696(02)13011-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040376015
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1016/s0092-8674(03)00847-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031003004
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1016/s0140-6736(05)66473-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019112974
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1073/pnas.2235735100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014717888
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1073/pnas.41.7.432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016425813
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1095/biolreprod.103.021147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007519570
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1634/stemcells.2004-0211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045135667
    218 rdf:type schema:CreativeWork
    219 https://www.grid.ac/institutes/grid.439113.d schema:alternateName WiCell
    220 schema:name WiCell Research Institute, PO Box 7365, Madison, Wisconsin 53707-7365, USA.
    221 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...