Derivation of human embryonic stem cells in defined conditions View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-02

AUTHORS

Tenneille E Ludwig, Mark E Levenstein, Jeffrey M Jones, W Travis Berggren, Erika R Mitchen, Jennifer L Frane, Leann J Crandall, Christine A Daigh, Kevin R Conard, Marian S Piekarczyk, Rachel A Llanas, James A Thomson

ABSTRACT

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions. More... »

PAGES

185-187

Journal

TITLE

Nature Biotechnology

ISSUE

2

VOLUME

24

Author Affiliations

Related Patents

  • Simplified Basic Media For Human Pluripotent Cell Culture
  • Generation Of Induced Pluripotent Stem Cells From Small Volumes Of Peripheral Blood
  • Stem Cell Defined Media For Xeno-Free And Feeder Free Conditions And Uses Thereof
  • A Method For Obtaining A Xeno-Free Hbs Cell Line
  • Somatic Cell Reprogramming By Retroviral Vectors Encoding Oct3/4. Klf4, C-Myc And Sox2
  • Substrates And Methods For Culturing Stem Cells
  • Methods And Products For Expressing Proteins In Cells
  • Cell Culture Substrate Comprising A Laminin And A Cadherin
  • Mesenchymal-Like Stem Cells Derived From Human Embryonic Stem Cells, Methods And Uses Thereof
  • Swellable (Meth)Acrylate Surfaces For Culturing Cells In Chemically Defined Media
  • Cell Culture Composition Comprising Laminin-521
  • Methods And Products For Expressing Proteins In Cells
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Method To Perform Beam-Type Collision-Activated Dissociation In The Pre-Existing Ion Injection Pathway Of A Mass Spectrometer
  • Medium For Growing Human Embryonic Stem Cells
  • Culture Medium Containing Gamma-Aminobutyric Acid, Pipecolic Acid Or Lithium For The Maintenance Of Stem Cells In An Undifferentiated State
  • Method For Extracellular Matrix Mediated Differentiation And Proliferation Of Stem Cells
  • Methods And Products For Transfecting Cells
  • Synthetic Surfaces For Culturing Stem Cell Derived Oligodendrocyte Progenitor Cells
  • Method To Perform Beam-Type Collision-Activated Dissociation In The Pre-Existing Ion Injection Pathway Of A Mass Spectrometer
  • Methods And Products For Nucleic Acid Production And Delivery
  • Methods And Platforms For Drug Discovery Using Induced Pluripotent Stem Cells
  • Human Blood-Brain Endothelial Cells Derived From Pluripotent Stem Cells And Blood-Brain Barrier Model Thereof
  • Defined Conditions For Human Embryonic Stem Cell Culture And Passage
  • Screening Methods For Human Embryonic Stem Cells
  • Methods For Producing Enucleated Erythroid Cells Derived From Pluripotent Stem Cells
  • Automated Method And Apparatus For Embryonic Stem Cell Culture
  • Scalable Primate Pluripotent Stem Cell Aggregate Suspension Culture And Differentiation Thereof
  • Method To Perform Beam-Type Collision-Activated Dissociation In The Pre-Existing Ion Injection Pathway Of A Mass Spectrometer
  • Generation Of Induced Pluripotent Stem Cells From Small Volumes Of Peripheral Blood
  • Ecm Composition, Tumor Microenvironment Platform And Methods Thereof
  • Methods Of Enhancing Genetic Diagnosis
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Primate Pluripotent Stem Cells Cultured In Medium Containing Gamma-Aminobutyric Acid, Pipecolic Acid And Lithium
  • Priming Of Pluripotent Stem Cells For Neural Differentiation
  • Methods For The Production Of Ips Cells Using Epstein-Barr (Ebv)-Based Reprogramming Vectors
  • Differentiation Of Pluripotent Cells
  • Growth Of Cells
  • Generating Pluripotent Cells De Novo
  • Methods And Products For Expressing Proteins In Cells
  • Chitosan-Alginate Scaffold Cell Culture System And Related Methods
  • Fgf Having Enhanced Stability
  • Methods Of Expanding Embryonic Stem Cells In A Suspension Culture
  • Methods And Materials For Obtaining Induced Pluripotent Stem Cells
  • Engineering A Heterogeneous Tissue From Pluripotent Stem Cells
  • Methods And Products For Transfection
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1177

    DOI

    http://dx.doi.org/10.1038/nbt1177

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030407065

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16388305


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Proliferation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Survival", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tissue Engineering", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "WiCell", 
              "id": "https://www.grid.ac/institutes/grid.439113.d", 
              "name": [
                "WiCell Research Institute, PO Box 7365, Madison, Wisconsin 53707-7365, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ludwig", 
            "givenName": "Tenneille E", 
            "id": "sg:person.01154664726.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154664726.94"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Levenstein", 
            "givenName": "Mark E", 
            "id": "sg:person.01145062633.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145062633.75"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Jones", 
            "givenName": "Jeffrey M", 
            "id": "sg:person.0721160752.90", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721160752.90"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Berggren", 
            "givenName": "W Travis", 
            "id": "sg:person.0735657217.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735657217.13"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Mitchen", 
            "givenName": "Erika R", 
            "type": "Person"
          }, 
          {
            "familyName": "Frane", 
            "givenName": "Jennifer L", 
            "id": "sg:person.01050140115.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050140115.64"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Crandall", 
            "givenName": "Leann J", 
            "type": "Person"
          }, 
          {
            "familyName": "Daigh", 
            "givenName": "Christine A", 
            "id": "sg:person.01164366515.87", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164366515.87"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Conard", 
            "givenName": "Kevin R", 
            "id": "sg:person.01327424433.69", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327424433.69"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Piekarczyk", 
            "givenName": "Marian S", 
            "id": "sg:person.0673607765.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673607765.00"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Llanas", 
            "givenName": "Rachel A", 
            "id": "sg:person.01346730315.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346730315.40"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Thomson", 
            "givenName": "James A", 
            "id": "sg:person.012266000337.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012266000337.10"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nm979", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000301825", 
              "https://doi.org/10.1038/nm979"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm979", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000301825", 
              "https://doi.org/10.1038/nm979"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0028-3908(86)90263-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002391209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0028-3908(86)90263-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002391209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt922", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005956847", 
              "https://doi.org/10.1038/nbt922"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt922", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005956847", 
              "https://doi.org/10.1038/nbt922"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1095/biolreprod.103.021147", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007519570"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.2235735100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014717888"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.41.7.432", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016425813"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0140-6736(05)66473-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019112974"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt761", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021609375", 
              "https://doi.org/10.1038/nbt761"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt761", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021609375", 
              "https://doi.org/10.1038/nbt761"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.282.5391.1145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028898955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029326356", 
              "https://doi.org/10.1038/nmeth744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029326356", 
              "https://doi.org/10.1038/nmeth744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(03)00847-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031003004"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1181", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033356897", 
              "https://doi.org/10.1038/nm1181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1181", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033356897", 
              "https://doi.org/10.1038/nm1181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0074-7696(02)13011-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040376015"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2004-0211", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045135667"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/dbio.2000.9912", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053693663"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2006-02", 
        "datePublishedReg": "2006-02-01", 
        "description": "We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt1177", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2622167", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "24"
          }
        ], 
        "name": "Derivation of human embryonic stem cells in defined conditions", 
        "pagination": "185-187", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "90db21e3da9b511c6ea4283692500f04cccc47c4dd2488c9d8c64ce5e555a3b5"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "16388305"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1177"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1030407065"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1177", 
          "https://app.dimensions.ai/details/publication/pub.1030407065"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:15", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_54014_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/nbt/journal/v24/n2/full/nbt1177.html"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1177'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1177'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1177'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1177'


     

    This table displays all metadata directly associated to this object as RDF triples.

    221 TRIPLES      21 PREDICATES      53 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1177 schema:about N029a3baf38684d07abff04381401779c
    2 N0c2598e2ffe548b1bf68936d733c61ba
    3 N16d93ea3ebd5410ca3c0972d30aaef0d
    4 N4ab0546ac1fc438ab5496292b78efe4e
    5 N5674b40186ae4230aec0aa5b97408992
    6 N6114f271675347deb7aee9743f82164a
    7 Na64497fc23fe4725b67650ae66501d6a
    8 Nb9939bafc61d4bc985e1ebeb2a306bbd
    9 Nfdb52119d3b34b068809ac93ea158d15
    10 anzsrc-for:06
    11 anzsrc-for:0601
    12 schema:author Nfc5abe7093da4b5ba94aa3f30e7dc958
    13 schema:citation sg:pub.10.1038/nbt761
    14 sg:pub.10.1038/nbt922
    15 sg:pub.10.1038/nm1181
    16 sg:pub.10.1038/nm979
    17 sg:pub.10.1038/nmeth744
    18 https://doi.org/10.1006/dbio.2000.9912
    19 https://doi.org/10.1016/0028-3908(86)90263-7
    20 https://doi.org/10.1016/s0074-7696(02)13011-7
    21 https://doi.org/10.1016/s0092-8674(03)00847-x
    22 https://doi.org/10.1016/s0140-6736(05)66473-2
    23 https://doi.org/10.1073/pnas.2235735100
    24 https://doi.org/10.1073/pnas.41.7.432
    25 https://doi.org/10.1095/biolreprod.103.021147
    26 https://doi.org/10.1126/science.282.5391.1145
    27 https://doi.org/10.1634/stemcells.2004-0211
    28 schema:datePublished 2006-02
    29 schema:datePublishedReg 2006-02-01
    30 schema:description We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
    31 schema:genre research_article
    32 schema:inLanguage en
    33 schema:isAccessibleForFree false
    34 schema:isPartOf N893138c1c59941b18af71b471367f909
    35 Ndffbb684af694059b168f0abf5d8800b
    36 sg:journal.1115214
    37 schema:name Derivation of human embryonic stem cells in defined conditions
    38 schema:pagination 185-187
    39 schema:productId N4b7b7324f895416c8a43d7bfdd2ca30d
    40 N71cd52412a124968b19b9b524f6ce39f
    41 Nb6c423e38cdd44d3b66f516ab04aec94
    42 Ncb581a1ec722479088e576374c741b10
    43 Nfd17ae045af54060851c1670e4829d81
    44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030407065
    45 https://doi.org/10.1038/nbt1177
    46 schema:sdDatePublished 2019-04-11T12:15
    47 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    48 schema:sdPublisher N04d38dddae1a4eb5ac3153d2bd83a375
    49 schema:url http://www.nature.com/nbt/journal/v24/n2/full/nbt1177.html
    50 sgo:license sg:explorer/license/
    51 sgo:sdDataset articles
    52 rdf:type schema:ScholarlyArticle
    53 N029a3baf38684d07abff04381401779c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    54 schema:name Humans
    55 rdf:type schema:DefinedTerm
    56 N04d38dddae1a4eb5ac3153d2bd83a375 schema:name Springer Nature - SN SciGraph project
    57 rdf:type schema:Organization
    58 N0c2598e2ffe548b1bf68936d733c61ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    59 schema:name Cell Differentiation
    60 rdf:type schema:DefinedTerm
    61 N0c350a814e5944149d8795b8ea040f21 rdf:first sg:person.01164366515.87
    62 rdf:rest Nddbe683de2bd4293a2902cfb232b0090
    63 N16d93ea3ebd5410ca3c0972d30aaef0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    64 schema:name Cell Survival
    65 rdf:type schema:DefinedTerm
    66 N21c7aa92f64e49d39e1a881261af2134 rdf:first sg:person.0735657217.13
    67 rdf:rest Nd7b0fd99d592463d9909ac88adf86f1e
    68 N303e2462bc2945fe8e9c0422094cee08 rdf:first sg:person.012266000337.10
    69 rdf:rest rdf:nil
    70 N4ab0546ac1fc438ab5496292b78efe4e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    71 schema:name Cell Line
    72 rdf:type schema:DefinedTerm
    73 N4b7b7324f895416c8a43d7bfdd2ca30d schema:name doi
    74 schema:value 10.1038/nbt1177
    75 rdf:type schema:PropertyValue
    76 N522e92c684fd4821931b9127534cda69 rdf:first sg:person.01050140115.64
    77 rdf:rest Nb253285ea3b3443c8d78635ad89ec9a7
    78 N5674b40186ae4230aec0aa5b97408992 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Culture Media
    80 rdf:type schema:DefinedTerm
    81 N6114f271675347deb7aee9743f82164a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Stem Cells
    83 rdf:type schema:DefinedTerm
    84 N672d97eb550b44fe8dcdd0f388ee23fd rdf:first sg:person.0721160752.90
    85 rdf:rest N21c7aa92f64e49d39e1a881261af2134
    86 N71cd52412a124968b19b9b524f6ce39f schema:name pubmed_id
    87 schema:value 16388305
    88 rdf:type schema:PropertyValue
    89 N81f11d669fc9432aa201c5102219dcf6 rdf:first sg:person.01145062633.75
    90 rdf:rest N672d97eb550b44fe8dcdd0f388ee23fd
    91 N893138c1c59941b18af71b471367f909 schema:volumeNumber 24
    92 rdf:type schema:PublicationVolume
    93 N8db1b9bf8c1645fabf13f724c258a8b7 rdf:first sg:person.0673607765.00
    94 rdf:rest Nf91120aca2794285a174c00b9ca8c51e
    95 Na64497fc23fe4725b67650ae66501d6a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Tissue Engineering
    97 rdf:type schema:DefinedTerm
    98 Nb253285ea3b3443c8d78635ad89ec9a7 rdf:first Nf283874c2cd84712b61234d240cd0e6f
    99 rdf:rest N0c350a814e5944149d8795b8ea040f21
    100 Nb6c423e38cdd44d3b66f516ab04aec94 schema:name nlm_unique_id
    101 schema:value 9604648
    102 rdf:type schema:PropertyValue
    103 Nb9939bafc61d4bc985e1ebeb2a306bbd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Cell Culture Techniques
    105 rdf:type schema:DefinedTerm
    106 Ncb581a1ec722479088e576374c741b10 schema:name dimensions_id
    107 schema:value pub.1030407065
    108 rdf:type schema:PropertyValue
    109 Nd7b0fd99d592463d9909ac88adf86f1e rdf:first Nff387191b7ec45119b06c4497ef5f1dd
    110 rdf:rest N522e92c684fd4821931b9127534cda69
    111 Nddbe683de2bd4293a2902cfb232b0090 rdf:first sg:person.01327424433.69
    112 rdf:rest N8db1b9bf8c1645fabf13f724c258a8b7
    113 Ndffbb684af694059b168f0abf5d8800b schema:issueNumber 2
    114 rdf:type schema:PublicationIssue
    115 Nf283874c2cd84712b61234d240cd0e6f schema:familyName Crandall
    116 schema:givenName Leann J
    117 rdf:type schema:Person
    118 Nf91120aca2794285a174c00b9ca8c51e rdf:first sg:person.01346730315.40
    119 rdf:rest N303e2462bc2945fe8e9c0422094cee08
    120 Nfc5abe7093da4b5ba94aa3f30e7dc958 rdf:first sg:person.01154664726.94
    121 rdf:rest N81f11d669fc9432aa201c5102219dcf6
    122 Nfd17ae045af54060851c1670e4829d81 schema:name readcube_id
    123 schema:value 90db21e3da9b511c6ea4283692500f04cccc47c4dd2488c9d8c64ce5e555a3b5
    124 rdf:type schema:PropertyValue
    125 Nfdb52119d3b34b068809ac93ea158d15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Cell Proliferation
    127 rdf:type schema:DefinedTerm
    128 Nff387191b7ec45119b06c4497ef5f1dd schema:familyName Mitchen
    129 schema:givenName Erika R
    130 rdf:type schema:Person
    131 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    132 schema:name Biological Sciences
    133 rdf:type schema:DefinedTerm
    134 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    135 schema:name Biochemistry and Cell Biology
    136 rdf:type schema:DefinedTerm
    137 sg:grant.2622167 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt1177
    138 rdf:type schema:MonetaryGrant
    139 sg:journal.1115214 schema:issn 1087-0156
    140 1546-1696
    141 schema:name Nature Biotechnology
    142 rdf:type schema:Periodical
    143 sg:person.01050140115.64 schema:familyName Frane
    144 schema:givenName Jennifer L
    145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050140115.64
    146 rdf:type schema:Person
    147 sg:person.01145062633.75 schema:familyName Levenstein
    148 schema:givenName Mark E
    149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145062633.75
    150 rdf:type schema:Person
    151 sg:person.01154664726.94 schema:affiliation https://www.grid.ac/institutes/grid.439113.d
    152 schema:familyName Ludwig
    153 schema:givenName Tenneille E
    154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154664726.94
    155 rdf:type schema:Person
    156 sg:person.01164366515.87 schema:familyName Daigh
    157 schema:givenName Christine A
    158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164366515.87
    159 rdf:type schema:Person
    160 sg:person.012266000337.10 schema:familyName Thomson
    161 schema:givenName James A
    162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012266000337.10
    163 rdf:type schema:Person
    164 sg:person.01327424433.69 schema:familyName Conard
    165 schema:givenName Kevin R
    166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327424433.69
    167 rdf:type schema:Person
    168 sg:person.01346730315.40 schema:familyName Llanas
    169 schema:givenName Rachel A
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346730315.40
    171 rdf:type schema:Person
    172 sg:person.0673607765.00 schema:familyName Piekarczyk
    173 schema:givenName Marian S
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673607765.00
    175 rdf:type schema:Person
    176 sg:person.0721160752.90 schema:familyName Jones
    177 schema:givenName Jeffrey M
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721160752.90
    179 rdf:type schema:Person
    180 sg:person.0735657217.13 schema:familyName Berggren
    181 schema:givenName W Travis
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735657217.13
    183 rdf:type schema:Person
    184 sg:pub.10.1038/nbt761 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021609375
    185 https://doi.org/10.1038/nbt761
    186 rdf:type schema:CreativeWork
    187 sg:pub.10.1038/nbt922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005956847
    188 https://doi.org/10.1038/nbt922
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1038/nm1181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033356897
    191 https://doi.org/10.1038/nm1181
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.1038/nm979 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000301825
    194 https://doi.org/10.1038/nm979
    195 rdf:type schema:CreativeWork
    196 sg:pub.10.1038/nmeth744 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029326356
    197 https://doi.org/10.1038/nmeth744
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1006/dbio.2000.9912 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053693663
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1016/0028-3908(86)90263-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002391209
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1016/s0074-7696(02)13011-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040376015
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1016/s0092-8674(03)00847-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031003004
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1016/s0140-6736(05)66473-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019112974
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1073/pnas.2235735100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014717888
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1073/pnas.41.7.432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016425813
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1095/biolreprod.103.021147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007519570
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1634/stemcells.2004-0211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045135667
    218 rdf:type schema:CreativeWork
    219 https://www.grid.ac/institutes/grid.439113.d schema:alternateName WiCell
    220 schema:name WiCell Research Institute, PO Box 7365, Madison, Wisconsin 53707-7365, USA.
    221 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...