Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-07

AUTHORS

Ganga Rao Davuluri, Ageeth van Tuinen, Paul D Fraser, Alessandro Manfredonia, Robert Newman, Diane Burgess, David A Brummell, Stephen R King, Joe Palys, John Uhlig, Peter M Bramley, Henk M J Pennings, Chris Bowler

ABSTRACT

Tomatoes are a principal dietary source of carotenoids and flavonoids, both of which are highly beneficial for human health. Overexpression of genes encoding biosynthetic enzymes or transcription factors have resulted in tomatoes with improved carotenoid or flavonoid content, but never with both. We attempted to increase tomato fruit nutritional value by suppressing an endogenous photomorphogenesis regulatory gene, DET1, using fruit-specific promoters combined with RNA interference (RNAi) technology. Molecular analysis indicated that DET1 transcripts were indeed specifically degraded in transgenic fruits. Both carotenoid and flavonoid contents were increased significantly, whereas other parameters of fruit quality were largely unchanged. These results demonstrate that manipulation of a plant regulatory gene can simultaneously influence the production of several phytonutrients generated from independent biosynthetic pathways, and provide a novel example of the use of organ-specific gene silencing to improve the nutritional value of plant-derived products. More... »

PAGES

890-895

Journal

TITLE

Nature Biotechnology

ISSUE

7

VOLUME

23

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt1108

DOI

http://dx.doi.org/10.1038/nbt1108

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018379429

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15951803


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