Use of Early Baculovirus Promoters for Continuous Expression and Efficient Processing of Foreign Gene Products in Stably Transformed Lepidopteran Cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-10

AUTHORS

D L Jarvis, J A Fleming, G R Kovacs, M D Summers, L A Guarino

ABSTRACT

Baculoviruses are currently used as vectors for the transient high-level expression of foreign gene products in insect cells. In this study, we demonstrate that baculoviruses can also be made to continuously express a foreign gene product by using the promoter from IE1, an immediate early viral gene, to produce stably-transformed insect cells. This approach gave levels of foreign gene expression lower than those usually obtained with the lytic baculovirus expression vector system. Expression, however, was continuous and stable, and a complex human glycoprotein (tissue plasminogen activator) was processed more efficiently. We conclude that stable transformation is a feasible approach for baculovirus-mediated foreign gene expression in lepidopteran cells, particularly for products that are relatively poorly-expressed and/or processed in lytically infected cells. More... »

PAGES

950

References to SciGraph publications

Journal

TITLE

Bio/Technology

ISSUE

10

VOLUME

8

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt1090-950

DOI

http://dx.doi.org/10.1038/nbt1090-950

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034312685

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1367473


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