Programmable ligand-controlled riboregulators of eukaryotic gene expression View Full Text


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Article Info

DATE

2005-03

AUTHORS

Travis S Bayer, Christina D Smolke

ABSTRACT

Recent studies have demonstrated the importance of noncoding RNA elements in regulating gene expression networks. We describe the design of a class of small trans-acting RNAs that directly regulate gene expression in a ligand-dependent manner. These allosteric riboregulators, which we call antiswitches, are made fully tunable and modular by rational design. They offer flexible control strategies by adopting active or inactive forms in response to ligand binding, depending on their design. They can be tailor-made to regulate the expression of target transcripts in response to different cellular effectors. Coupled with in vitro selection technologies for generating nucleic acid ligand-binding species, antiswitches present a platform for programming cellular behavior and genetic networks with respect to cellular state and environmental stimuli. More... »

PAGES

337

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  • Journal

    TITLE

    Nature Biotechnology

    ISSUE

    3

    VOLUME

    23

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1069

    DOI

    http://dx.doi.org/10.1038/nbt1069

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1037508478

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15723047


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