RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-12

AUTHORS

Robert S Allen, Anthony G Millgate, Julie A Chitty, Jennifer Thisleton, James A C Miller, Anthony J Fist, Wayne L Gerlach, Philip J Larkin

ABSTRACT

We report on the silencing of codeinone reductase (COR) in the opium poppy, Papaver somniferum, using a chimeric hairpin RNA construct designed to silence all members of the multigene COR family through RNA interference (RNAi). After gene silencing, the precursor alkaloid (S)-reticuline-seven enzymatic steps upstream of codeinone-accumulated in transgenic plants at the expense of morphine, codeine, oripavine and thebaine. Methylated derivatives of reticuline also accumulated. Analysis verified loss of Cor gene transcript, appearance of 22-mer degradation products and reduction of enzyme activity. The surprising accumulation of (S)-reticuline suggests a feedback mechanism preventing intermediates from general benzylisoquinoline synthesis entering the morphine-specific branch. However transcript levels for seven other enzymes in the pathway, both before and after (S)-reticuline, were unaffected. This is the first report of gene silencing in transgenic opium poppy and of metabolic engineering to cause the high-yield accumulation of the nonnarcotic alkaloid reticuline. More... »

PAGES

1559-1566

Journal

TITLE

Nature Biotechnology

ISSUE

12

VOLUME

22

Related Patents

  • Benzylisoquinoline Alkaloids (Bia) Producing Microbes, And Methods Of Making And Using The Same
  • Method Of Altering The Alkaloid Composition In Poppy Plants
  • Tobacco Inbred And Hybrid Plants And Tobacco Products Made Thereof
  • Compositions And Methods For Producing Benzylisoquinoline Alkaloids
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Papaver Somniferum With High Concentration Of Codeine
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Tobacco Inbred Plants Ncbex1f, Ncbex1ms, And Nc Ex90
  • Tobacco Plants Having Reduced Nicotine Demethylase Activity
  • Benzylisoquinoline Alkaloids (Bia) Producing Microbes, And Methods Of Making And Using The Same
  • Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes
  • Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes
  • Compositions And Methods For Producing Benzylisoquinoline Alkaloids
  • Cytochrome P450 Fusion Protein
  • Tobacco Nicotine Demethylase Genomic Clone And Uses Thereof
  • Method For Production Of Alkaloid
  • Polynucleotides Having A Nucleotide Sequence That Encodes A Polypeptide Having Mov34 Family Activity
  • Regulatory Regions From Papaveraceae
  • Method For Producing Alkaloids
  • Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes
  • Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes
  • Nicotiana Nucleic Acid Molecules And Uses Thereof
  • Tobacco Inbred And Hybrid Plants And Tobacco Products Made Thereof
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Modulating Plant Alkaloids
  • Nicotiana Nucleic Acid Molecules And Uses Thereof
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Tobacco Nicotine Demethylase Genomic Clone And Uses Thereof
  • Tobacco Inbred Plants K326 Src, Cms K326 Src, K346 Src, Cms K346 Src, Nc1562-1 Src, Nctg-61 Src, Cms Nctg-61 Src And Hybrid Nc196 Src
  • Compositions And Methods For Minimizing Nornicotine Synthesis In Tobacco
  • Nicotiana Nucleic Acid Molecules And Uses Thereof
  • Nicotiana Nucleic Acid Molecules And Uses Thereof
  • Tobacco Inbred Plants Albex1f And Albex1ms
  • Tobacco Inbred And Hybrid Plants And Tobacco Products Made Thereof
  • Novel Cytochrome P450 Fusion Protein
  • Identification Of Terpenoid-Biosynthesis Related Regulatory Protein-Regulatory Region Associations
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Sequence-Determined Dna Encoding Ap2 Domain Polypeptides
  • Tobacco Nicotine Demethylase Genomic Clone And Uses Thereof
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1033

    DOI

    http://dx.doi.org/10.1038/nbt1033

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1013537601

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15543134


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Alcohol Oxidoreductases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Alkaloids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Benzylisoquinolines", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Morphine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Papaver", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA Interference", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Plant Industry", 
              "id": "https://www.grid.ac/institutes/grid.417667.5", 
              "name": [
                "CSIRO Plant Industry, PO Box 1600, Canberra, Australian Capital Territory 2601, Australia."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Allen", 
            "givenName": "Robert S", 
            "id": "sg:person.01126552133.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126552133.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Plant Industry", 
              "id": "https://www.grid.ac/institutes/grid.417667.5", 
              "name": [
                "CSIRO Plant Industry, PO Box 1600, Canberra, Australian Capital Territory 2601, Australia."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Millgate", 
            "givenName": "Anthony G", 
            "id": "sg:person.01001752313.62", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001752313.62"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Plant Industry", 
              "id": "https://www.grid.ac/institutes/grid.417667.5", 
              "name": [
                "CSIRO Plant Industry, PO Box 1600, Canberra, Australian Capital Territory 2601, Australia."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chitty", 
            "givenName": "Julie A", 
            "id": "sg:person.0704337026.67", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704337026.67"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Plant Industry", 
              "id": "https://www.grid.ac/institutes/grid.417667.5", 
              "name": [
                "CSIRO Plant Industry, PO Box 1600, Canberra, Australian Capital Territory 2601, Australia."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Thisleton", 
            "givenName": "Jennifer", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Tasmanian Alkaloids Pty Ltd, PO Box 130, Westbury, Tasmania 7303, Australia."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Miller", 
            "givenName": "James A C", 
            "id": "sg:person.01020565426.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01020565426.57"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Tasmanian Alkaloids Pty Ltd, PO Box 130, Westbury, Tasmania 7303, Australia."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fist", 
            "givenName": "Anthony J", 
            "id": "sg:person.01166604547.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01166604547.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johnson & Johnson (Australia)", 
              "id": "https://www.grid.ac/institutes/grid.473170.6", 
              "name": [
                "Johnson & Johnson Research Pty Ltd, Locked Bag 4555, Strawberry Hills, New South Wales 2012, Australia."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gerlach", 
            "givenName": "Wayne L", 
            "id": "sg:person.01173652631.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173652631.44"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Plant Industry", 
              "id": "https://www.grid.ac/institutes/grid.417667.5", 
              "name": [
                "CSIRO Plant Industry, PO Box 1600, Canberra, Australian Capital Territory 2601, Australia."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Larkin", 
            "givenName": "Philip J", 
            "id": "sg:person.01203127226.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203127226.75"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1071/fp02166", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004027038"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1369-5266(02)00236-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004919152"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00446367", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006455833", 
              "https://doi.org/10.1007/bf00446367"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00446367", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006455833", 
              "https://doi.org/10.1007/bf00446367"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1071/fp03126", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008926276"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0031-9422(92)80111-q", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009983779"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1995.132_1.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011441723"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1091-963", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012182392", 
              "https://doi.org/10.1038/nbt1091-963"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1021199311049", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012348963", 
              "https://doi.org/10.1023/a:1021199311049"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.96.22.12929", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013107961"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1998.00085.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014721867"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1998.00085.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014721867"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1998.00085.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014721867"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01209037", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016333164", 
              "https://doi.org/10.1007/bf01209037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01209037", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016333164", 
              "https://doi.org/10.1007/bf01209037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0079-9920(03)80022-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016537236"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1999.00470.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016689426"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/9780470650622.ch7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018430647"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m102688200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019830660"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1071/fp02033", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020258264"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0031-9422(03)00292-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021463190"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0031-9422(03)00292-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021463190"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.015396", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022959586"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.004077", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029819405"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pca.673", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029840981"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.2003.01928.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030691267"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00024382-199909000-00001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032792941"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00024382-199909000-00001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032792941"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.88.22.9969", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032862629"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/431413a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034521229", 
              "https://doi.org/10.1038/431413a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/431413a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034521229", 
              "https://doi.org/10.1038/431413a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.010741", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035475090"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00425-002-0867-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036692531", 
              "https://doi.org/10.1007/s00425-002-0867-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ptr.929", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042660215"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1071/fp02167", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044623220"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s004250100582", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045343104", 
              "https://doi.org/10.1007/s004250100582"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0405704101", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046870467"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.phytochem.2004.02.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047781485"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.2001.01105.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047855591"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1248/bpb.20.586", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050900101"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.92.1.75", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051506337"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.12.9.1689", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053224394"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/np980144f", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056239241"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/np980144f", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056239241"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1055/s-2002-32546", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1057415526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1055/s-2006-957367", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1057470391"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1055/s-2007-969727", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1057502494"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1055/s-2007-969921", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1057502687"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2004-12", 
        "datePublishedReg": "2004-12-01", 
        "description": "We report on the silencing of codeinone reductase (COR) in the opium poppy, Papaver somniferum, using a chimeric hairpin RNA construct designed to silence all members of the multigene COR family through RNA interference (RNAi). After gene silencing, the precursor alkaloid (S)-reticuline-seven enzymatic steps upstream of codeinone-accumulated in transgenic plants at the expense of morphine, codeine, oripavine and thebaine. Methylated derivatives of reticuline also accumulated. Analysis verified loss of Cor gene transcript, appearance of 22-mer degradation products and reduction of enzyme activity. The surprising accumulation of (S)-reticuline suggests a feedback mechanism preventing intermediates from general benzylisoquinoline synthesis entering the morphine-specific branch. However transcript levels for seven other enzymes in the pathway, both before and after (S)-reticuline, were unaffected. This is the first report of gene silencing in transgenic opium poppy and of metabolic engineering to cause the high-yield accumulation of the nonnarcotic alkaloid reticuline.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt1033", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "12", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "22"
          }
        ], 
        "name": "RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy", 
        "pagination": "1559-1566", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "407d780a04304b5ec943a52d1b360d22bcb131f39b0750be2929d833ced21e93"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "15543134"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1033"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1013537601"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1033", 
          "https://app.dimensions.ai/details/publication/pub.1013537601"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:25", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87104_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nbt1033"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1033'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1033'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1033'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1033'


     

    This table displays all metadata directly associated to this object as RDF triples.

    291 TRIPLES      21 PREDICATES      79 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1033 schema:about N0348ce51a4994ed5a17b6e2c49b68715
    2 N578a405c163c4cbfa0aa3a0fb38d5d8e
    3 N8634025a63b34f94a898e9dd97d0b5a5
    4 N89cdb1c24b84430e8fe945192a71b4d3
    5 Nb0f627f800d649fcada520630d7b4ebd
    6 Nb62a4d7ba6554c2aa124af97ad4a2438
    7 Nbd7be9831ad84f3eae19752fdac9d164
    8 Nd4b31941ec7b4a4cac9b8805c6887bea
    9 Nde6960849ee84cb697007e182acf821f
    10 Nff60b88f27ca47339f1ede19a770c309
    11 anzsrc-for:06
    12 anzsrc-for:0604
    13 schema:author Na4a3c479094d4c51b3b12eb01f02d61f
    14 schema:citation sg:pub.10.1007/bf00446367
    15 sg:pub.10.1007/bf01209037
    16 sg:pub.10.1007/s00425-002-0867-5
    17 sg:pub.10.1007/s004250100582
    18 sg:pub.10.1023/a:1021199311049
    19 sg:pub.10.1038/431413a
    20 sg:pub.10.1038/nbt1091-963
    21 https://doi.org/10.1002/9780470650622.ch7
    22 https://doi.org/10.1002/pca.673
    23 https://doi.org/10.1002/ptr.929
    24 https://doi.org/10.1016/0031-9422(92)80111-q
    25 https://doi.org/10.1016/j.phytochem.2004.02.015
    26 https://doi.org/10.1016/s0031-9422(03)00292-9
    27 https://doi.org/10.1016/s0079-9920(03)80022-9
    28 https://doi.org/10.1016/s1369-5266(02)00236-4
    29 https://doi.org/10.1021/np980144f
    30 https://doi.org/10.1046/j.1365-313x.1998.00085.x
    31 https://doi.org/10.1046/j.1365-313x.1999.00470.x
    32 https://doi.org/10.1046/j.1365-313x.2001.01105.x
    33 https://doi.org/10.1046/j.1365-313x.2003.01928.x
    34 https://doi.org/10.1055/s-2002-32546
    35 https://doi.org/10.1055/s-2006-957367
    36 https://doi.org/10.1055/s-2007-969727
    37 https://doi.org/10.1055/s-2007-969921
    38 https://doi.org/10.1071/fp02033
    39 https://doi.org/10.1071/fp02166
    40 https://doi.org/10.1071/fp02167
    41 https://doi.org/10.1071/fp03126
    42 https://doi.org/10.1073/pnas.0405704101
    43 https://doi.org/10.1073/pnas.88.22.9969
    44 https://doi.org/10.1073/pnas.92.1.75
    45 https://doi.org/10.1073/pnas.96.22.12929
    46 https://doi.org/10.1074/jbc.m102688200
    47 https://doi.org/10.1097/00024382-199909000-00001
    48 https://doi.org/10.1104/pp.010741
    49 https://doi.org/10.1105/tpc.004077
    50 https://doi.org/10.1105/tpc.015396
    51 https://doi.org/10.1105/tpc.12.9.1689
    52 https://doi.org/10.1111/j.1432-1033.1995.132_1.x
    53 https://doi.org/10.1248/bpb.20.586
    54 schema:datePublished 2004-12
    55 schema:datePublishedReg 2004-12-01
    56 schema:description We report on the silencing of codeinone reductase (COR) in the opium poppy, Papaver somniferum, using a chimeric hairpin RNA construct designed to silence all members of the multigene COR family through RNA interference (RNAi). After gene silencing, the precursor alkaloid (S)-reticuline-seven enzymatic steps upstream of codeinone-accumulated in transgenic plants at the expense of morphine, codeine, oripavine and thebaine. Methylated derivatives of reticuline also accumulated. Analysis verified loss of Cor gene transcript, appearance of 22-mer degradation products and reduction of enzyme activity. The surprising accumulation of (S)-reticuline suggests a feedback mechanism preventing intermediates from general benzylisoquinoline synthesis entering the morphine-specific branch. However transcript levels for seven other enzymes in the pathway, both before and after (S)-reticuline, were unaffected. This is the first report of gene silencing in transgenic opium poppy and of metabolic engineering to cause the high-yield accumulation of the nonnarcotic alkaloid reticuline.
    57 schema:genre research_article
    58 schema:inLanguage en
    59 schema:isAccessibleForFree false
    60 schema:isPartOf Nbaeecf4dffac45f5b4cf755d7225ceaa
    61 Ncc917394a37249dabd017acaac73959c
    62 sg:journal.1115214
    63 schema:name RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy
    64 schema:pagination 1559-1566
    65 schema:productId N3628c043ecbf4096ad22bac528ded465
    66 N392148d4e37e4f71961d50fd3bbfdb22
    67 N7cb07388afea4443bee893ae3b14bc1d
    68 Nba6480f97b3c42309f3afb7d5166f114
    69 Nfc34bc2e527e438c98a4660db0fddb42
    70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013537601
    71 https://doi.org/10.1038/nbt1033
    72 schema:sdDatePublished 2019-04-11T12:25
    73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    74 schema:sdPublisher Nc06b2421ba4b4331980f200bdbeb4967
    75 schema:url http://www.nature.com/articles/nbt1033
    76 sgo:license sg:explorer/license/
    77 sgo:sdDataset articles
    78 rdf:type schema:ScholarlyArticle
    79 N0348ce51a4994ed5a17b6e2c49b68715 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Cells, Cultured
    81 rdf:type schema:DefinedTerm
    82 N0f09329c845446448ba3f9f584e43988 rdf:first Nc65466a48b964c06aeca1cfe5fb660c0
    83 rdf:rest Nc11d9035f4104679b9ef10d1d6df35d4
    84 N303e295b5e644145805ab83171bc87f0 rdf:first sg:person.01173652631.44
    85 rdf:rest N5336af499501409fa1acf74b211f2935
    86 N3628c043ecbf4096ad22bac528ded465 schema:name doi
    87 schema:value 10.1038/nbt1033
    88 rdf:type schema:PropertyValue
    89 N392148d4e37e4f71961d50fd3bbfdb22 schema:name pubmed_id
    90 schema:value 15543134
    91 rdf:type schema:PropertyValue
    92 N3a5d6c83a874416d9d2895eb1084a0ba rdf:first sg:person.01166604547.25
    93 rdf:rest N303e295b5e644145805ab83171bc87f0
    94 N5336af499501409fa1acf74b211f2935 rdf:first sg:person.01203127226.75
    95 rdf:rest rdf:nil
    96 N578a405c163c4cbfa0aa3a0fb38d5d8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Plants, Genetically Modified
    98 rdf:type schema:DefinedTerm
    99 N7cb07388afea4443bee893ae3b14bc1d schema:name dimensions_id
    100 schema:value pub.1013537601
    101 rdf:type schema:PropertyValue
    102 N8634025a63b34f94a898e9dd97d0b5a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Alcohol Oxidoreductases
    104 rdf:type schema:DefinedTerm
    105 N89cdb1c24b84430e8fe945192a71b4d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    106 schema:name Morphine
    107 rdf:type schema:DefinedTerm
    108 N99dbb7f0a0a94fc3adfd1ebde9abd303 schema:name Tasmanian Alkaloids Pty Ltd, PO Box 130, Westbury, Tasmania 7303, Australia.
    109 rdf:type schema:Organization
    110 Na4a3c479094d4c51b3b12eb01f02d61f rdf:first sg:person.01126552133.20
    111 rdf:rest Ndea0c870e2084aafb799dc2116739360
    112 Nb0f627f800d649fcada520630d7b4ebd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Benzylisoquinolines
    114 rdf:type schema:DefinedTerm
    115 Nb62a4d7ba6554c2aa124af97ad4a2438 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Genetic Engineering
    117 rdf:type schema:DefinedTerm
    118 Nba6480f97b3c42309f3afb7d5166f114 schema:name nlm_unique_id
    119 schema:value 9604648
    120 rdf:type schema:PropertyValue
    121 Nbaeecf4dffac45f5b4cf755d7225ceaa schema:issueNumber 12
    122 rdf:type schema:PublicationIssue
    123 Nbd7be9831ad84f3eae19752fdac9d164 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name RNA Interference
    125 rdf:type schema:DefinedTerm
    126 Nc06b2421ba4b4331980f200bdbeb4967 schema:name Springer Nature - SN SciGraph project
    127 rdf:type schema:Organization
    128 Nc11d9035f4104679b9ef10d1d6df35d4 rdf:first sg:person.01020565426.57
    129 rdf:rest N3a5d6c83a874416d9d2895eb1084a0ba
    130 Nc65466a48b964c06aeca1cfe5fb660c0 schema:affiliation https://www.grid.ac/institutes/grid.417667.5
    131 schema:familyName Thisleton
    132 schema:givenName Jennifer
    133 rdf:type schema:Person
    134 Ncc917394a37249dabd017acaac73959c schema:volumeNumber 22
    135 rdf:type schema:PublicationVolume
    136 Nd4b31941ec7b4a4cac9b8805c6887bea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases
    138 rdf:type schema:DefinedTerm
    139 Nde6960849ee84cb697007e182acf821f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Alkaloids
    141 rdf:type schema:DefinedTerm
    142 Ndea0c870e2084aafb799dc2116739360 rdf:first sg:person.01001752313.62
    143 rdf:rest Ne0a45f8662e44bb8b3f47b66f37cc272
    144 Ne0a45f8662e44bb8b3f47b66f37cc272 rdf:first sg:person.0704337026.67
    145 rdf:rest N0f09329c845446448ba3f9f584e43988
    146 Ne6dfd46a197c4549a9a560bf63b2c547 schema:name Tasmanian Alkaloids Pty Ltd, PO Box 130, Westbury, Tasmania 7303, Australia.
    147 rdf:type schema:Organization
    148 Nfc34bc2e527e438c98a4660db0fddb42 schema:name readcube_id
    149 schema:value 407d780a04304b5ec943a52d1b360d22bcb131f39b0750be2929d833ced21e93
    150 rdf:type schema:PropertyValue
    151 Nff60b88f27ca47339f1ede19a770c309 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Papaver
    153 rdf:type schema:DefinedTerm
    154 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    155 schema:name Biological Sciences
    156 rdf:type schema:DefinedTerm
    157 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    158 schema:name Genetics
    159 rdf:type schema:DefinedTerm
    160 sg:journal.1115214 schema:issn 1087-0156
    161 1546-1696
    162 schema:name Nature Biotechnology
    163 rdf:type schema:Periodical
    164 sg:person.01001752313.62 schema:affiliation https://www.grid.ac/institutes/grid.417667.5
    165 schema:familyName Millgate
    166 schema:givenName Anthony G
    167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001752313.62
    168 rdf:type schema:Person
    169 sg:person.01020565426.57 schema:affiliation N99dbb7f0a0a94fc3adfd1ebde9abd303
    170 schema:familyName Miller
    171 schema:givenName James A C
    172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01020565426.57
    173 rdf:type schema:Person
    174 sg:person.01126552133.20 schema:affiliation https://www.grid.ac/institutes/grid.417667.5
    175 schema:familyName Allen
    176 schema:givenName Robert S
    177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126552133.20
    178 rdf:type schema:Person
    179 sg:person.01166604547.25 schema:affiliation Ne6dfd46a197c4549a9a560bf63b2c547
    180 schema:familyName Fist
    181 schema:givenName Anthony J
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01166604547.25
    183 rdf:type schema:Person
    184 sg:person.01173652631.44 schema:affiliation https://www.grid.ac/institutes/grid.473170.6
    185 schema:familyName Gerlach
    186 schema:givenName Wayne L
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173652631.44
    188 rdf:type schema:Person
    189 sg:person.01203127226.75 schema:affiliation https://www.grid.ac/institutes/grid.417667.5
    190 schema:familyName Larkin
    191 schema:givenName Philip J
    192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203127226.75
    193 rdf:type schema:Person
    194 sg:person.0704337026.67 schema:affiliation https://www.grid.ac/institutes/grid.417667.5
    195 schema:familyName Chitty
    196 schema:givenName Julie A
    197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704337026.67
    198 rdf:type schema:Person
    199 sg:pub.10.1007/bf00446367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006455833
    200 https://doi.org/10.1007/bf00446367
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1007/bf01209037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016333164
    203 https://doi.org/10.1007/bf01209037
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1007/s00425-002-0867-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036692531
    206 https://doi.org/10.1007/s00425-002-0867-5
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1007/s004250100582 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045343104
    209 https://doi.org/10.1007/s004250100582
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1023/a:1021199311049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012348963
    212 https://doi.org/10.1023/a:1021199311049
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1038/431413a schema:sameAs https://app.dimensions.ai/details/publication/pub.1034521229
    215 https://doi.org/10.1038/431413a
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1038/nbt1091-963 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012182392
    218 https://doi.org/10.1038/nbt1091-963
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1002/9780470650622.ch7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018430647
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1002/pca.673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029840981
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1002/ptr.929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042660215
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1016/0031-9422(92)80111-q schema:sameAs https://app.dimensions.ai/details/publication/pub.1009983779
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1016/j.phytochem.2004.02.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047781485
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1016/s0031-9422(03)00292-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021463190
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1016/s0079-9920(03)80022-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016537236
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1016/s1369-5266(02)00236-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004919152
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1021/np980144f schema:sameAs https://app.dimensions.ai/details/publication/pub.1056239241
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1046/j.1365-313x.1998.00085.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014721867
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1046/j.1365-313x.1999.00470.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016689426
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1046/j.1365-313x.2001.01105.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1047855591
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1046/j.1365-313x.2003.01928.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030691267
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1055/s-2002-32546 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057415526
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1055/s-2006-957367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057470391
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1055/s-2007-969727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057502494
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1055/s-2007-969921 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057502687
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1071/fp02033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020258264
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1071/fp02166 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004027038
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1071/fp02167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044623220
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1071/fp03126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008926276
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1073/pnas.0405704101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046870467
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1073/pnas.88.22.9969 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032862629
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1073/pnas.92.1.75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051506337
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1073/pnas.96.22.12929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013107961
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1074/jbc.m102688200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019830660
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1097/00024382-199909000-00001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032792941
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1104/pp.010741 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035475090
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1105/tpc.004077 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029819405
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1105/tpc.015396 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022959586
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1105/tpc.12.9.1689 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053224394
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1111/j.1432-1033.1995.132_1.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011441723
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1248/bpb.20.586 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050900101
    285 rdf:type schema:CreativeWork
    286 https://www.grid.ac/institutes/grid.417667.5 schema:alternateName Plant Industry
    287 schema:name CSIRO Plant Industry, PO Box 1600, Canberra, Australian Capital Territory 2601, Australia.
    288 rdf:type schema:Organization
    289 https://www.grid.ac/institutes/grid.473170.6 schema:alternateName Johnson & Johnson (Australia)
    290 schema:name Johnson & Johnson Research Pty Ltd, Locked Bag 4555, Strawberry Hills, New South Wales 2012, Australia.
    291 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...