Plant cell cultures for the production of recombinant proteins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-11

AUTHORS

Stephan Hellwig, Jürgen Drossard, Richard M Twyman, Rainer Fischer

ABSTRACT

The use of whole plants for the synthesis of recombinant proteins has received a great deal of attention recently because of advantages in economy, scalability and safety compared with traditional microbial and mammalian production systems. However, production systems that use whole plants lack several of the intrinsic benefits of cultured cells, including the precise control over growth conditions, batch-to-batch product consistency, a high level of containment and the ability to produce recombinant proteins in compliance with good manufacturing practice. Plant cell cultures combine the merits of whole-plant systems with those of microbial and animal cell cultures, and already have an established track record for the production of valuable therapeutic secondary metabolites. Although no recombinant proteins have yet been produced commercially using plant cell cultures, there have been many proof-of-principle studies and several companies are investigating the commercial feasibility of such production systems. More... »

PAGES

1415-1422

References to SciGraph publications

  • 1999-10. Production of functional human α1-antitrypsin by plant cell culture in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2002-11. Stimulation of murine granulocyte macrophage-colony stimulating factor production by Pluronic F-68 and polyethylene glycol in transgenic Nicotiana tabacum cell culture in BIOTECHNOLOGY LETTERS
  • 2001-09. The effect of immobilization on recombinant protein production in plant cell culture in PLANT CELL REPORTS
  • 2000-07. Engineering the plant cell factory for secondary metabolite production in TRANSGENIC RESEARCH
  • 1995-03. Characterization of a human glycoprotein (erythropoietin) produced in cultured tobacco cells in PLANT MOLECULAR BIOLOGY
  • 2001-08-09. Integrated Bioprocessing for Plant Cell Cultures in PLANT CELLS
  • 1992-07. Continuous culture of suspended plant cells in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1995-02. Bioprocessing technology for plant cell suspension cultures in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1989-09. Cell and tissue cultures ofCatharanthus roseus (L.) G. Don: a literature survey in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1999-12. Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies in TRANSGENIC RESEARCH
  • 2002-03. Plant tissue culture. Biotechnology. Milestones in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1996-02. The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco in PLANT MOLECULAR BIOLOGY
  • 2000-08. Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures in BIOTECHNOLOGY LETTERS
  • 1997-08. Plant cell suspension culture in a bench-scale fermenter with a newly designed membrane stirrer for bubble-free aeration in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1976-07. Plant tissue culture media in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1990-03. Production of Correctly Processed Human Serum Albumin in Transgenic Plants in BIO/TECHNOLOGY
  • 1997-01. The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody production from plant suspension cultures in BIOTECHNOLOGY LETTERS
  • 1995-05. Effects of dimethyl sulfoxide on heavy chain monoclonal antibody production from plant cell culture in BIOTECHNOLOGY LETTERS
  • 1993. Design of reactors for plant cells and organs in BIOPROCESS DESIGN AND CONTROL
  • 2003-09. Production and secretion of biologically active human granulocyte-macrophage colony stimulating factor in transgenic tomato suspension cultures in BIOTECHNOLOGY LETTERS
  • 2002-06. Expression of functional recombinant human lysozyme in transgenic rice cell culture in TRANSGENIC RESEARCH
  • 1993-11. Secretion of a functional single-chain Fv protein in transgenic tobacco plants and cell suspension cultures in PLANT MOLECULAR BIOLOGY
  • 2003-10. Genetic modification: The production of recombinant pharmaceutical proteins in plants in NATURE REVIEWS GENETICS
  • 1988-06. Growth and nicotinate biotransformation in batch cultured and airlift fermenter grown soybean cell suspension cultures in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2000-06. A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice in TRANSGENIC RESEARCH
  • 1990-05. Growth and hyoscyamine production of ‘hairy root’ cultures of Datura stramonium in a modified stirred tank reactor in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Journal

    TITLE

    Nature Biotechnology

    ISSUE

    11

    VOLUME

    22

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1027

    DOI

    http://dx.doi.org/10.1038/nbt1027

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1041248860

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15529167


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biological Products", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation, Plant", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Enhancement", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Signal Transduction", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Fraunhofer Institute for Molecular Biology and Applied Ecology", 
              "id": "https://www.grid.ac/institutes/grid.418010.c", 
              "name": [
                "Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Worringerweg 1, D-52074 Aachen, Germany."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hellwig", 
            "givenName": "Stephan", 
            "id": "sg:person.0744523701.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744523701.34"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Drossard", 
            "givenName": "J\u00fcrgen", 
            "id": "sg:person.01153672674.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153672674.45"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Twyman", 
            "givenName": "Richard M", 
            "id": "sg:person.01044515747.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044515747.54"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Fischer", 
            "givenName": "Rainer", 
            "id": "sg:person.0771473755.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771473755.26"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1006/prep.1998.0993", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000270325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(95)00057-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000347927"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bp0001516", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001239167"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tibtech.2003.10.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002261672"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0074-7696(08)62452-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002404574"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00021540", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003118519", 
              "https://doi.org/10.1007/bf00021540"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00021540", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003118519", 
              "https://doi.org/10.1007/bf00021540"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260281014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004122397"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1018383524389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004491447", 
              "https://doi.org/10.1023/a:1018383524389"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02796489", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004580541", 
              "https://doi.org/10.1007/bf02796489"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02796489", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004580541", 
              "https://doi.org/10.1007/bf02796489"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1389-1723(99)80036-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004675455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1515/bc.1999.102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005870042"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260260107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007525949"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1008976219939", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007529989", 
              "https://doi.org/10.1023/a:1008976219939"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-1656(02)00044-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007944637"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02823058", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010440271", 
              "https://doi.org/10.1007/bf02823058"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02823058", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010440271", 
              "https://doi.org/10.1007/bf02823058"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02783455", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011199158", 
              "https://doi.org/10.1007/bf02783455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02783455", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011199158", 
              "https://doi.org/10.1007/bf02783455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.10524", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012145688"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260350311", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012405634"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260350311", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012405634"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-0290(19970605)54:5<401::aid-bit1>3.0.co;2-i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014460613"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.pbi.2004.01.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014874939"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1470-8744.2002.tb01186.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015287966"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1008966404981", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015445466", 
              "https://doi.org/10.1023/a:1008966404981"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00132011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015936968", 
              "https://doi.org/10.1007/bf00132011"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00132011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015936968", 
              "https://doi.org/10.1007/bf00132011"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002530051554", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016340389", 
              "https://doi.org/10.1007/s002530051554"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002990100354", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017320918", 
              "https://doi.org/10.1007/s002990100354"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002990100354", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017320918", 
              "https://doi.org/10.1007/s002990100354"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jmv.20077", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017928821"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0168-9452(95)05694-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020406279"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/glycob/9.4.365", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021583835"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/prep.1996.0030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022558713"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/prep.2001.1580", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023074189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0006-291x(03)01335-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023356137"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0006-291x(03)01335-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023356137"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bp010104t", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023589523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023703429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/prep.1998.0872", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023755364"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1749-6632.1966.tb41193.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025473128"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.10532", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025609866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bfb0007198", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027230463", 
              "https://doi.org/10.1007/bfb0007198"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0141-0229(01)00331-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027506092"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.10528", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027784641"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/02648725.2001.10648017", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028346383"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0958-1669(00)00086-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028708275"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1005605214703", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028739478", 
              "https://doi.org/10.1023/a:1005605214703"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/prep.2000.1232", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029390361"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(94)90016-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029531568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(94)90016-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029531568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0390-217", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030366342", 
              "https://doi.org/10.1038/nbt0390-217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.96.8.4692", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030570740"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00020889", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031737983", 
              "https://doi.org/10.1007/bf00020889"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00020889", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031737983", 
              "https://doi.org/10.1007/bf00020889"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1015663706259", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034928158", 
              "https://doi.org/10.1023/a:1015663706259"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1079/ivp2001281", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035052870", 
              "https://doi.org/10.1079/ivp2001281"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1025409927790", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035317334", 
              "https://doi.org/10.1023/a:1025409927790"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0031-9422(93)85517-u", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035859965"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bst024199s", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037351252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bst024199s", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037351252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1432-1327.1999.00435.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038094553"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1360-1385(01)02193-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040540077"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00268188", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041093778", 
              "https://doi.org/10.1007/bf00268188"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00268188", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041093778", 
              "https://doi.org/10.1007/bf00268188"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb.57.1249", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041181881"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.pep.2003.07.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041203748"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.pep.2003.07.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041203748"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.10444", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042101612"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-0290(19970120)53:2<214::aid-bit12>3.0.co;2-k", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043745716"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(90)90071-w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044675563"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(90)90071-w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044675563"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1994.6050625.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045251204"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-1656(97)00163-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045479039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-1759(99)00058-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046946038"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(91)90104-i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047581161"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(91)90104-i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047581161"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.pbi.2004.01.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047623478"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1008969031219", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047898081", 
              "https://doi.org/10.1023/a:1008969031219"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/3-540-45302-4_3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048149073", 
              "https://doi.org/10.1007/3-540-45302-4_3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/3-540-45302-4_3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048149073", 
              "https://doi.org/10.1007/3-540-45302-4_3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00176513", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049356944", 
              "https://doi.org/10.1007/bf00176513"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00043397", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050583998", 
              "https://doi.org/10.1007/bf00043397"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00043397", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050583998", 
              "https://doi.org/10.1007/bf00043397"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg1177", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050745230", 
              "https://doi.org/10.1038/nrg1177"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg1177", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050745230", 
              "https://doi.org/10.1038/nrg1177"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0958-1669(95)80034-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051331860"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00019011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051335121", 
              "https://doi.org/10.1007/bf00019011"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00019011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051335121", 
              "https://doi.org/10.1007/bf00019011"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0958-1669(05)80031-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051841533"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1470-8744.1999.tb00899.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052313414"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002530051030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052635126", 
              "https://doi.org/10.1007/s002530051030"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1020609221148", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053178616", 
              "https://doi.org/10.1023/a:1020609221148"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bc970107k", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055156193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bc970107k", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055156193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.159.3814.540", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062493268"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.205.4411.1144", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062520025"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2174/1381612054367535", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069165573"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1990.tb07504.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078477431"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2004-11", 
        "datePublishedReg": "2004-11-01", 
        "description": "The use of whole plants for the synthesis of recombinant proteins has received a great deal of attention recently because of advantages in economy, scalability and safety compared with traditional microbial and mammalian production systems. However, production systems that use whole plants lack several of the intrinsic benefits of cultured cells, including the precise control over growth conditions, batch-to-batch product consistency, a high level of containment and the ability to produce recombinant proteins in compliance with good manufacturing practice. Plant cell cultures combine the merits of whole-plant systems with those of microbial and animal cell cultures, and already have an established track record for the production of valuable therapeutic secondary metabolites. Although no recombinant proteins have yet been produced commercially using plant cell cultures, there have been many proof-of-principle studies and several companies are investigating the commercial feasibility of such production systems.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt1027", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "11", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "22"
          }
        ], 
        "name": "Plant cell cultures for the production of recombinant proteins", 
        "pagination": "1415-1422", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "c47f1204f5f9ee1a53fbfeb640165d5096cc4b26c401ae30cc99d87b34f458db"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "15529167"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1027"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1041248860"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1027", 
          "https://app.dimensions.ai/details/publication/pub.1041248860"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:13", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53998_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/nbt/journal/v22/n11/full/nbt1027.html"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1027'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1027'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1027'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1027'


     

    This table displays all metadata directly associated to this object as RDF triples.

    392 TRIPLES      21 PREDICATES      119 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1027 schema:about N08e2d555ad0c43cbbf69c7e6cf458521
    2 N5e3212c08afb4b42b692a4d68c53d26d
    3 N61dce89c772c43ee9e0ba20a1560394c
    4 N6642137567c0422aa208b64f74893f0d
    5 N6a939262d06a42118057bae12a66ac34
    6 Na4c7e7c06ef24f05936a6862b212deaf
    7 Nc472c8198998431bb395806639175f07
    8 Nca3601129b814bd88a3061ade4d35faf
    9 Nef7d88d716974676a3fcbc77c253b659
    10 anzsrc-for:06
    11 anzsrc-for:0607
    12 schema:author Ndc50a59005a74b35926b943af87e7142
    13 schema:citation sg:pub.10.1007/3-540-45302-4_3
    14 sg:pub.10.1007/bf00019011
    15 sg:pub.10.1007/bf00020889
    16 sg:pub.10.1007/bf00021540
    17 sg:pub.10.1007/bf00043397
    18 sg:pub.10.1007/bf00132011
    19 sg:pub.10.1007/bf00176513
    20 sg:pub.10.1007/bf00268188
    21 sg:pub.10.1007/bf02783455
    22 sg:pub.10.1007/bf02796489
    23 sg:pub.10.1007/bf02823058
    24 sg:pub.10.1007/bfb0007198
    25 sg:pub.10.1007/s002530051030
    26 sg:pub.10.1007/s002530051554
    27 sg:pub.10.1007/s002990100354
    28 sg:pub.10.1023/a:1005605214703
    29 sg:pub.10.1023/a:1008966404981
    30 sg:pub.10.1023/a:1008969031219
    31 sg:pub.10.1023/a:1008976219939
    32 sg:pub.10.1023/a:1015663706259
    33 sg:pub.10.1023/a:1018383524389
    34 sg:pub.10.1023/a:1020609221148
    35 sg:pub.10.1023/a:1025409927790
    36 sg:pub.10.1038/nbt0390-217
    37 sg:pub.10.1038/nrg1177
    38 sg:pub.10.1079/ivp2001281
    39 https://doi.org/10.1002/(sici)1097-0290(19970120)53:2<214::aid-bit12>3.0.co;2-k
    40 https://doi.org/10.1002/(sici)1097-0290(19970605)54:5<401::aid-bit1>3.0.co;2-i
    41 https://doi.org/10.1002/bit.10444
    42 https://doi.org/10.1002/bit.10524
    43 https://doi.org/10.1002/bit.10528
    44 https://doi.org/10.1002/bit.10532
    45 https://doi.org/10.1002/bit.260260107
    46 https://doi.org/10.1002/bit.260281014
    47 https://doi.org/10.1002/bit.260350311
    48 https://doi.org/10.1002/j.1460-2075.1990.tb07504.x
    49 https://doi.org/10.1002/jmv.20077
    50 https://doi.org/10.1006/prep.1996.0030
    51 https://doi.org/10.1006/prep.1998.0872
    52 https://doi.org/10.1006/prep.1998.0993
    53 https://doi.org/10.1006/prep.2000.1232
    54 https://doi.org/10.1006/prep.2001.1580
    55 https://doi.org/10.1016/0031-9422(93)85517-u
    56 https://doi.org/10.1016/0141-0229(90)90071-w
    57 https://doi.org/10.1016/0141-0229(91)90104-i
    58 https://doi.org/10.1016/0141-0229(94)90016-7
    59 https://doi.org/10.1016/0141-0229(95)00057-7
    60 https://doi.org/10.1016/0168-9452(95)05694-7
    61 https://doi.org/10.1016/0958-1669(95)80034-4
    62 https://doi.org/10.1016/j.pbi.2004.01.007
    63 https://doi.org/10.1016/j.pbi.2004.01.015
    64 https://doi.org/10.1016/j.pep.2003.07.004
    65 https://doi.org/10.1016/j.tibtech.2003.10.002
    66 https://doi.org/10.1016/s0006-291x(03)01335-4
    67 https://doi.org/10.1016/s0022-1759(99)00058-7
    68 https://doi.org/10.1016/s0074-7696(08)62452-3
    69 https://doi.org/10.1016/s0141-0229(01)00331-3
    70 https://doi.org/10.1016/s0168-1656(02)00044-5
    71 https://doi.org/10.1016/s0168-1656(97)00163-6
    72 https://doi.org/10.1016/s0958-1669(00)00086-0
    73 https://doi.org/10.1016/s0958-1669(05)80031-x
    74 https://doi.org/10.1016/s1360-1385(01)02193-8
    75 https://doi.org/10.1016/s1389-1723(99)80036-x
    76 https://doi.org/10.1021/bc970107k
    77 https://doi.org/10.1021/bp0001516
    78 https://doi.org/10.1021/bp010104t
    79 https://doi.org/10.1042/bst024199s
    80 https://doi.org/10.1046/j.1365-313x.1994.6050625.x
    81 https://doi.org/10.1046/j.1432-1327.1999.00435.x
    82 https://doi.org/10.1073/pnas.96.8.4692
    83 https://doi.org/10.1080/02648725.2001.10648017
    84 https://doi.org/10.1093/glycob/9.4.365
    85 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
    86 https://doi.org/10.1111/j.1470-8744.1999.tb00899.x
    87 https://doi.org/10.1111/j.1470-8744.2002.tb01186.x
    88 https://doi.org/10.1111/j.1749-6632.1966.tb41193.x
    89 https://doi.org/10.1126/science.159.3814.540
    90 https://doi.org/10.1126/science.205.4411.1144
    91 https://doi.org/10.1271/bbb.57.1249
    92 https://doi.org/10.1515/bc.1999.102
    93 https://doi.org/10.2174/1381612054367535
    94 schema:datePublished 2004-11
    95 schema:datePublishedReg 2004-11-01
    96 schema:description The use of whole plants for the synthesis of recombinant proteins has received a great deal of attention recently because of advantages in economy, scalability and safety compared with traditional microbial and mammalian production systems. However, production systems that use whole plants lack several of the intrinsic benefits of cultured cells, including the precise control over growth conditions, batch-to-batch product consistency, a high level of containment and the ability to produce recombinant proteins in compliance with good manufacturing practice. Plant cell cultures combine the merits of whole-plant systems with those of microbial and animal cell cultures, and already have an established track record for the production of valuable therapeutic secondary metabolites. Although no recombinant proteins have yet been produced commercially using plant cell cultures, there have been many proof-of-principle studies and several companies are investigating the commercial feasibility of such production systems.
    97 schema:genre research_article
    98 schema:inLanguage en
    99 schema:isAccessibleForFree false
    100 schema:isPartOf N057e8f7b25874e88aa5f541e82672089
    101 N9f29b990fa1146d9805c41bbf30edd41
    102 sg:journal.1115214
    103 schema:name Plant cell cultures for the production of recombinant proteins
    104 schema:pagination 1415-1422
    105 schema:productId N43db581b8f754fbc9571c65b2209e224
    106 N8d662f04cc7943eb9589cfe097b28536
    107 N95524287ca3b478ca9a719e64ac8d85a
    108 Nd9e421dc226845818157b1d2d637c3cf
    109 Ne39a42422e264676a07ce1bef234b495
    110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041248860
    111 https://doi.org/10.1038/nbt1027
    112 schema:sdDatePublished 2019-04-11T12:13
    113 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    114 schema:sdPublisher N88d297023e5b421e9e17a05e35b4395d
    115 schema:url http://www.nature.com/nbt/journal/v22/n11/full/nbt1027.html
    116 sgo:license sg:explorer/license/
    117 sgo:sdDataset articles
    118 rdf:type schema:ScholarlyArticle
    119 N057e8f7b25874e88aa5f541e82672089 schema:volumeNumber 22
    120 rdf:type schema:PublicationVolume
    121 N08e2d555ad0c43cbbf69c7e6cf458521 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Cell Culture Techniques
    123 rdf:type schema:DefinedTerm
    124 N43db581b8f754fbc9571c65b2209e224 schema:name pubmed_id
    125 schema:value 15529167
    126 rdf:type schema:PropertyValue
    127 N5e3212c08afb4b42b692a4d68c53d26d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Signal Transduction
    129 rdf:type schema:DefinedTerm
    130 N61dce89c772c43ee9e0ba20a1560394c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    131 schema:name Plants
    132 rdf:type schema:DefinedTerm
    133 N6642137567c0422aa208b64f74893f0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Plant Proteins
    135 rdf:type schema:DefinedTerm
    136 N6a939262d06a42118057bae12a66ac34 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Recombinant Proteins
    138 rdf:type schema:DefinedTerm
    139 N88d297023e5b421e9e17a05e35b4395d schema:name Springer Nature - SN SciGraph project
    140 rdf:type schema:Organization
    141 N8d662f04cc7943eb9589cfe097b28536 schema:name readcube_id
    142 schema:value c47f1204f5f9ee1a53fbfeb640165d5096cc4b26c401ae30cc99d87b34f458db
    143 rdf:type schema:PropertyValue
    144 N95524287ca3b478ca9a719e64ac8d85a schema:name doi
    145 schema:value 10.1038/nbt1027
    146 rdf:type schema:PropertyValue
    147 N9f29b990fa1146d9805c41bbf30edd41 schema:issueNumber 11
    148 rdf:type schema:PublicationIssue
    149 Na2ee611ba1e4454b9b9756a9ca4792bb rdf:first sg:person.01044515747.54
    150 rdf:rest Nbbc95a4adc2b426783a476d933bd0d6e
    151 Na4c7e7c06ef24f05936a6862b212deaf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Biological Products
    153 rdf:type schema:DefinedTerm
    154 Nbbc95a4adc2b426783a476d933bd0d6e rdf:first sg:person.0771473755.26
    155 rdf:rest rdf:nil
    156 Nc472c8198998431bb395806639175f07 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    157 schema:name Protein Engineering
    158 rdf:type schema:DefinedTerm
    159 Nca3601129b814bd88a3061ade4d35faf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    160 schema:name Gene Expression Regulation, Plant
    161 rdf:type schema:DefinedTerm
    162 Ncc6fbadd6adb4bcebdd59ea35a91598c rdf:first sg:person.01153672674.45
    163 rdf:rest Na2ee611ba1e4454b9b9756a9ca4792bb
    164 Nd9e421dc226845818157b1d2d637c3cf schema:name dimensions_id
    165 schema:value pub.1041248860
    166 rdf:type schema:PropertyValue
    167 Ndc50a59005a74b35926b943af87e7142 rdf:first sg:person.0744523701.34
    168 rdf:rest Ncc6fbadd6adb4bcebdd59ea35a91598c
    169 Ne39a42422e264676a07ce1bef234b495 schema:name nlm_unique_id
    170 schema:value 9604648
    171 rdf:type schema:PropertyValue
    172 Nef7d88d716974676a3fcbc77c253b659 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    173 schema:name Genetic Enhancement
    174 rdf:type schema:DefinedTerm
    175 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    176 schema:name Biological Sciences
    177 rdf:type schema:DefinedTerm
    178 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    179 schema:name Plant Biology
    180 rdf:type schema:DefinedTerm
    181 sg:journal.1115214 schema:issn 1087-0156
    182 1546-1696
    183 schema:name Nature Biotechnology
    184 rdf:type schema:Periodical
    185 sg:person.01044515747.54 schema:familyName Twyman
    186 schema:givenName Richard M
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044515747.54
    188 rdf:type schema:Person
    189 sg:person.01153672674.45 schema:familyName Drossard
    190 schema:givenName Jürgen
    191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153672674.45
    192 rdf:type schema:Person
    193 sg:person.0744523701.34 schema:affiliation https://www.grid.ac/institutes/grid.418010.c
    194 schema:familyName Hellwig
    195 schema:givenName Stephan
    196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744523701.34
    197 rdf:type schema:Person
    198 sg:person.0771473755.26 schema:familyName Fischer
    199 schema:givenName Rainer
    200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771473755.26
    201 rdf:type schema:Person
    202 sg:pub.10.1007/3-540-45302-4_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048149073
    203 https://doi.org/10.1007/3-540-45302-4_3
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1007/bf00019011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051335121
    206 https://doi.org/10.1007/bf00019011
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1007/bf00020889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031737983
    209 https://doi.org/10.1007/bf00020889
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1007/bf00021540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003118519
    212 https://doi.org/10.1007/bf00021540
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1007/bf00043397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050583998
    215 https://doi.org/10.1007/bf00043397
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1007/bf00132011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015936968
    218 https://doi.org/10.1007/bf00132011
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1007/bf00176513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049356944
    221 https://doi.org/10.1007/bf00176513
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1007/bf00268188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041093778
    224 https://doi.org/10.1007/bf00268188
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1007/bf02783455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011199158
    227 https://doi.org/10.1007/bf02783455
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1007/bf02796489 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004580541
    230 https://doi.org/10.1007/bf02796489
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1007/bf02823058 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010440271
    233 https://doi.org/10.1007/bf02823058
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1007/bfb0007198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027230463
    236 https://doi.org/10.1007/bfb0007198
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1007/s002530051030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052635126
    239 https://doi.org/10.1007/s002530051030
    240 rdf:type schema:CreativeWork
    241 sg:pub.10.1007/s002530051554 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016340389
    242 https://doi.org/10.1007/s002530051554
    243 rdf:type schema:CreativeWork
    244 sg:pub.10.1007/s002990100354 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017320918
    245 https://doi.org/10.1007/s002990100354
    246 rdf:type schema:CreativeWork
    247 sg:pub.10.1023/a:1005605214703 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028739478
    248 https://doi.org/10.1023/a:1005605214703
    249 rdf:type schema:CreativeWork
    250 sg:pub.10.1023/a:1008966404981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015445466
    251 https://doi.org/10.1023/a:1008966404981
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1023/a:1008969031219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047898081
    254 https://doi.org/10.1023/a:1008969031219
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1023/a:1008976219939 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007529989
    257 https://doi.org/10.1023/a:1008976219939
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1023/a:1015663706259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034928158
    260 https://doi.org/10.1023/a:1015663706259
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1023/a:1018383524389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004491447
    263 https://doi.org/10.1023/a:1018383524389
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1023/a:1020609221148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053178616
    266 https://doi.org/10.1023/a:1020609221148
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1023/a:1025409927790 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035317334
    269 https://doi.org/10.1023/a:1025409927790
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1038/nbt0390-217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030366342
    272 https://doi.org/10.1038/nbt0390-217
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1038/nrg1177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050745230
    275 https://doi.org/10.1038/nrg1177
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1079/ivp2001281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035052870
    278 https://doi.org/10.1079/ivp2001281
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1002/(sici)1097-0290(19970120)53:2<214::aid-bit12>3.0.co;2-k schema:sameAs https://app.dimensions.ai/details/publication/pub.1043745716
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1002/(sici)1097-0290(19970605)54:5<401::aid-bit1>3.0.co;2-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1014460613
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1002/bit.10444 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042101612
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1002/bit.10524 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012145688
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1002/bit.10528 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027784641
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1002/bit.10532 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025609866
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1002/bit.260260107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007525949
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1002/bit.260281014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004122397
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1002/bit.260350311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012405634
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1002/j.1460-2075.1990.tb07504.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078477431
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1002/jmv.20077 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017928821
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1006/prep.1996.0030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022558713
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1006/prep.1998.0872 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023755364
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1006/prep.1998.0993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000270325
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1006/prep.2000.1232 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029390361
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1006/prep.2001.1580 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023074189
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1016/0031-9422(93)85517-u schema:sameAs https://app.dimensions.ai/details/publication/pub.1035859965
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.1016/0141-0229(90)90071-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1044675563
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.1016/0141-0229(91)90104-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1047581161
    317 rdf:type schema:CreativeWork
    318 https://doi.org/10.1016/0141-0229(94)90016-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029531568
    319 rdf:type schema:CreativeWork
    320 https://doi.org/10.1016/0141-0229(95)00057-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000347927
    321 rdf:type schema:CreativeWork
    322 https://doi.org/10.1016/0168-9452(95)05694-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020406279
    323 rdf:type schema:CreativeWork
    324 https://doi.org/10.1016/0958-1669(95)80034-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051331860
    325 rdf:type schema:CreativeWork
    326 https://doi.org/10.1016/j.pbi.2004.01.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014874939
    327 rdf:type schema:CreativeWork
    328 https://doi.org/10.1016/j.pbi.2004.01.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047623478
    329 rdf:type schema:CreativeWork
    330 https://doi.org/10.1016/j.pep.2003.07.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041203748
    331 rdf:type schema:CreativeWork
    332 https://doi.org/10.1016/j.tibtech.2003.10.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002261672
    333 rdf:type schema:CreativeWork
    334 https://doi.org/10.1016/s0006-291x(03)01335-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023356137
    335 rdf:type schema:CreativeWork
    336 https://doi.org/10.1016/s0022-1759(99)00058-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046946038
    337 rdf:type schema:CreativeWork
    338 https://doi.org/10.1016/s0074-7696(08)62452-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002404574
    339 rdf:type schema:CreativeWork
    340 https://doi.org/10.1016/s0141-0229(01)00331-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027506092
    341 rdf:type schema:CreativeWork
    342 https://doi.org/10.1016/s0168-1656(02)00044-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007944637
    343 rdf:type schema:CreativeWork
    344 https://doi.org/10.1016/s0168-1656(97)00163-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045479039
    345 rdf:type schema:CreativeWork
    346 https://doi.org/10.1016/s0958-1669(00)00086-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028708275
    347 rdf:type schema:CreativeWork
    348 https://doi.org/10.1016/s0958-1669(05)80031-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051841533
    349 rdf:type schema:CreativeWork
    350 https://doi.org/10.1016/s1360-1385(01)02193-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040540077
    351 rdf:type schema:CreativeWork
    352 https://doi.org/10.1016/s1389-1723(99)80036-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004675455
    353 rdf:type schema:CreativeWork
    354 https://doi.org/10.1021/bc970107k schema:sameAs https://app.dimensions.ai/details/publication/pub.1055156193
    355 rdf:type schema:CreativeWork
    356 https://doi.org/10.1021/bp0001516 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001239167
    357 rdf:type schema:CreativeWork
    358 https://doi.org/10.1021/bp010104t schema:sameAs https://app.dimensions.ai/details/publication/pub.1023589523
    359 rdf:type schema:CreativeWork
    360 https://doi.org/10.1042/bst024199s schema:sameAs https://app.dimensions.ai/details/publication/pub.1037351252
    361 rdf:type schema:CreativeWork
    362 https://doi.org/10.1046/j.1365-313x.1994.6050625.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1045251204
    363 rdf:type schema:CreativeWork
    364 https://doi.org/10.1046/j.1432-1327.1999.00435.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038094553
    365 rdf:type schema:CreativeWork
    366 https://doi.org/10.1073/pnas.96.8.4692 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030570740
    367 rdf:type schema:CreativeWork
    368 https://doi.org/10.1080/02648725.2001.10648017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028346383
    369 rdf:type schema:CreativeWork
    370 https://doi.org/10.1093/glycob/9.4.365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021583835
    371 rdf:type schema:CreativeWork
    372 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
    373 rdf:type schema:CreativeWork
    374 https://doi.org/10.1111/j.1470-8744.1999.tb00899.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052313414
    375 rdf:type schema:CreativeWork
    376 https://doi.org/10.1111/j.1470-8744.2002.tb01186.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015287966
    377 rdf:type schema:CreativeWork
    378 https://doi.org/10.1111/j.1749-6632.1966.tb41193.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1025473128
    379 rdf:type schema:CreativeWork
    380 https://doi.org/10.1126/science.159.3814.540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062493268
    381 rdf:type schema:CreativeWork
    382 https://doi.org/10.1126/science.205.4411.1144 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062520025
    383 rdf:type schema:CreativeWork
    384 https://doi.org/10.1271/bbb.57.1249 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041181881
    385 rdf:type schema:CreativeWork
    386 https://doi.org/10.1515/bc.1999.102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005870042
    387 rdf:type schema:CreativeWork
    388 https://doi.org/10.2174/1381612054367535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069165573
    389 rdf:type schema:CreativeWork
    390 https://www.grid.ac/institutes/grid.418010.c schema:alternateName Fraunhofer Institute for Molecular Biology and Applied Ecology
    391 schema:name Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Worringerweg 1, D-52074 Aachen, Germany.
    392 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...