Feeder-free growth of undifferentiated human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-10

AUTHORS

Chunhui Xu, Margaret S. Inokuma, Jerrod Denham, Kathaleen Golds, Pratima Kundu, Joseph D. Gold, Melissa K. Carpenter

ABSTRACT

Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least 130 population doublings. In this system, hES cells are cultured on Matrigel or laminin in medium conditioned by MEF. The hES cells maintained on feeders or off feeders express integrin alpha6 and beta1, which may form a laminin-specific receptor. The hES cell populations in feeder-free conditions maintained a normal karyotype, stable proliferation rate, and high telomerase activity. Similar to cells cultured on feeders, hES cells maintained under feeder-free conditions expressed OCT-4, hTERT, alkaline phosphatase, and surface markers including SSEA-4, Tra 1-60, and Tra 1-81. In addition, hES cells maintained without direct feeder contact formed teratomas in SCID/beige mice and differentiated in vitro into cells from all three germ layers. Thus, the cells retain fundamental characteristics of hES cells in this culture system and are suitable for scaleup production. More... »

PAGES

971-974

Journal

TITLE

Nature Biotechnology

ISSUE

10

VOLUME

19

Author Affiliations

Related Patents

  • Modified Polynucleotides Encoding Apoptosis Inducing Factor 1
  • Enhancement Of Placental Stem Cell Potency Using Modulatory Rna Molecules
  • Compositions And Methods For Reprogramming Eukaryotic Cells
  • Modified Polynucleotides For The Production Of Nuclear Proteins
  • Highly Efficient Methods For Reprogramming Differentiated Cells And For Generating Animals And Embryonic Stem Cells From Reprogrammed Cells
  • Modified Polynucleotides Encoding Cited4
  • Modified Polynucleotides Encoding Septin-4
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Stem Cells Culture Systems
  • Substrates And Methods For Culturing Stem Cells
  • Renovation And Repopulation Of Decellularized Tissues And Cadaveric Organs By Stem Cells
  • Derivation Of Embryonic Stem Cells
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Medium Comprising Transforming Growth Factor Beta 1 And Basic Fibroblast Growth Factor
  • Methods For Culturing Undifferentiated Cells Using Sustained Release Compositions
  • Generation Of Vascular Progenitor Cells
  • Treatment Of Sarcoidosis Using Placental Stem Cells
  • Placental Stem Cells
  • Methods Of Expanding Embryonic Stem Cells In A Suspension Culture
  • Immunomodulation Using Placental Stem Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Polypeptide Composition And Culture Method For Pluripotent Stem Cell Using Same
  • Methods And Compositions For Promoting Survival And Proliferation Of Endothelial Cells And Stimulating Angiogenesis
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Rpe Cell Populations And Methods Of Generating Same
  • Isolation Of Inner Cell Mass For The Establishment Of Human Embryonic Stem Cell (Hesc) Lines
  • Method For Extracellular Matrix Mediated Differentiation And Proliferation Of Stem Cells
  • Modified Polynucleotides For Treating Protein Deficiency
  • Synthetic Surfaces For Culturing Stem Cell Derived Oligodendrocyte Progenitor Cells
  • Differentiation And Expansion Of Endothelial Cells From Pluripotent Stem Cells And The In Vitro Formation Of Vasculature Like Structures
  • Neural Cell Populations From Primate Pluripotent Stem Cells
  • Differentiation Of Primate Pluripotent Stem Cells To Hematopoietic Lineage Cells
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Isolated Stem Cell Comprising A Xic Flanking Region Transgene
  • Definitive Endoderm
  • Stem Cells Culture Systems
  • Primate Pluripotent Stem Cells Produced By Somatic Cell Nuclear Transfer
  • Preprimitive Streak And Mesendoderm Cells
  • Composition And Method For Enabling Proliferation Of Pluripotent Human Stem Cells
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Screening Methods For Human Embryonic Stem Cells
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Making And Using In Vitro-Synthesized Ssrna For Introducing Into Mammalian Cells To Induce A Biological Or Biochemical Effect
  • Method For Culturing Stem Cells
  • Scalable Primate Pluripotent Stem Cell Aggregate Suspension Culture And Differentiation Thereof
  • Placental Stem Cell Populations
  • Definitive Endoderm
  • Tissue Culture Medium Comprising Transforming Growth Factor Beta 1 And Basic Fibroblast Growth Factor
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Pluripotency Determining Factors And Uses Thereof
  • Primate Pluripotent Stem Cells Cultured In Medium Containing Gamma-Aminobutyric Acid, Pipecolic Acid And Lithium
  • Modified Polynucleotides For The Production Of Proteins Associated With Human Disease
  • Single Pluripotent Stem Cell Culture
  • Differentiation Of Primate Pluripotent Stem Cells To Cardiomyocyte-Lineage Cells
  • Treatment Of Multiple Sclerosis Using Placental Stem Cells
  • Formulation And Delivery Of Plga Microspheres
  • Compositions And Methods Of Altering Cholesterol Levels
  • Cultivation Of Human Embryonic Stem Cells In The Absence Of Feeder Cells Or Without Conditioned Medium
  • Compositions For The In Vitro Derivation And Culture Of Embryonic Stem (Es) Cell Lines With Germline Transmission Capability And For The Culture Of Adult Stem Cells
  • Methods Of Inducing Pluripotency
  • Method For Making High Purity Cardiomyocyte Preparations Suitable For Regenerative Medicine
  • Compositions And Methods For Reprogramming Eukaryotic Cells
  • Differentiation Of Primate Pluripotent Stem Cells To Cardiomyocyte-Lineage Cells
  • Methods Of Expanding Embryonic Stem Cells In A Suspension Culture
  • Isolated Primate Embryonic Cells Derived From Extended Blastocysts
  • Co-Culturing Mammalian Embryonic Stem Cells With Human Foreskin Fibroblasts
  • Methods Of Regulating Differentiation In Stem Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Modified Polynucleotides Encoding Copper Metabolism (Murr1) Domain Containing 1
  • Modified Polynucleotides Encoding Basic Helix-Loop-Helix Family Member E41
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Compositions For The In Vitro Derivation And Culture Of Embryonic Stem (Es) Cell Lines With Germline Transmission Capability And For The Culture Of Adult Stem Cells
  • Modified Polynucleotides For The Production Of Proteins
  • Photolithography On Shrink Film
  • Composition And Method For Proliferating Undifferentiated Stem Cells
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Composition And Method For Enabling Proliferation Of Pluripotent Stem Cells
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1001-971

    DOI

    http://dx.doi.org/10.1038/nbt1001-971

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014515248

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11581665


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Division", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Coculture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Collagen", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media, Conditioned", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Drug Combinations", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Embryo, Mammalian", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Embryo, Nonmammalian", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Flow Cytometry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Glycosphingolipids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Laminin", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Proteoglycans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Reverse Transcriptase Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stage-Specific Embryonic Antigens", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cells", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Geron (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418219.7", 
              "name": [
                "Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Chunhui", 
            "id": "sg:person.01207330163.59", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207330163.59"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Geron (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418219.7", 
              "name": [
                "Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Inokuma", 
            "givenName": "Margaret S.", 
            "id": "sg:person.01022674377.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022674377.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Geron (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418219.7", 
              "name": [
                "Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Denham", 
            "givenName": "Jerrod", 
            "id": "sg:person.0726523201.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726523201.14"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Geron (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418219.7", 
              "name": [
                "Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Golds", 
            "givenName": "Kathaleen", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Geron (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418219.7", 
              "name": [
                "Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kundu", 
            "givenName": "Pratima", 
            "id": "sg:person.01061360765.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061360765.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Geron (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418219.7", 
              "name": [
                "Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gold", 
            "givenName": "Joseph D.", 
            "id": "sg:person.0630535243.69", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630535243.69"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Geron (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418219.7", 
              "name": [
                "Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Carpenter", 
            "givenName": "Melissa K.", 
            "id": "sg:person.01254176236.61", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254176236.61"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/0092-8674(92)90115-s", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007440282"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0012-1606(83)90183-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010044484"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/336688a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010804374", 
              "https://doi.org/10.1038/336688a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/336688a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010804374", 
              "https://doi.org/10.1038/336688a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3109/15419069309095680", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013747112"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1297-498", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028381516", 
              "https://doi.org/10.1038/ng1297-498"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.282.5391.1145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028898955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/74447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033604670", 
              "https://doi.org/10.1038/74447"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/74447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033604670", 
              "https://doi.org/10.1038/74447"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/336684a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038677733", 
              "https://doi.org/10.1038/336684a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/336684a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038677733", 
              "https://doi.org/10.1038/336684a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.cb.02.110186.000331", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039638042"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.115.3.843", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046025583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1172/jci112887", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049043498"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.279.5349.349", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051728512"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/dbio.2000.9912", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053693663"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00267a025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055167840"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.7605428", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062648139"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2001-10", 
        "datePublishedReg": "2001-10-01", 
        "description": "Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least 130 population doublings. In this system, hES cells are cultured on Matrigel or laminin in medium conditioned by MEF. The hES cells maintained on feeders or off feeders express integrin alpha6 and beta1, which may form a laminin-specific receptor. The hES cell populations in feeder-free conditions maintained a normal karyotype, stable proliferation rate, and high telomerase activity. Similar to cells cultured on feeders, hES cells maintained under feeder-free conditions expressed OCT-4, hTERT, alkaline phosphatase, and surface markers including SSEA-4, Tra 1-60, and Tra 1-81. In addition, hES cells maintained without direct feeder contact formed teratomas in SCID/beige mice and differentiated in vitro into cells from all three germ layers. Thus, the cells retain fundamental characteristics of hES cells in this culture system and are suitable for scaleup production.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt1001-971", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "10", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "19"
          }
        ], 
        "name": "Feeder-free growth of undifferentiated human embryonic stem cells", 
        "pagination": "971-974", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "4526360f7d7ed5ae0cde25e497712ca1157d4fea60a759f826a47bf956e49c2b"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "11581665"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1001-971"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1014515248"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1001-971", 
          "https://app.dimensions.ai/details/publication/pub.1014515248"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:13", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53999_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nbt1001-971"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1001-971'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1001-971'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1001-971'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1001-971'


     

    This table displays all metadata directly associated to this object as RDF triples.

    227 TRIPLES      21 PREDICATES      61 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1001-971 schema:about N04eccc5f075e4b6699516828a6398b18
    2 N16beb1caffd943d4a5b64b6534c42f62
    3 N320786f55fef403fab9ccc1911ada2fe
    4 N324fb7f7c6b94a82b02ab4c30c3da9ea
    5 N39a5649d8f404eacbdd24e35cb9d68d2
    6 N5628a58c2a644a90a236bbd7bfd98d66
    7 N65fc0a6b8322489e84869473b4de0817
    8 N692d34274884432abad13b10f7a0b260
    9 N70abdb7b23a949fabf3a6fb686422cb8
    10 Nad873a62f7774c2e8b00446f370cd4b7
    11 Nb823f1ed5e2d499eaa25bf92ea379279
    12 Nc6ceeb9b956c40bab7c1b3982b3b9a04
    13 Nc7c081bc6caf44c0ae0593f1d01df7d8
    14 Nc95e9d728c5244e6aa2c23fe3ce750ba
    15 Ncea7e7ae581b4a1ab3ec9e7aa77fa9f2
    16 Nd79b02fe80bf496887f7d0425534aa47
    17 Nfc6314e4299c4d7fbf4e7b64fc3aa78c
    18 anzsrc-for:06
    19 anzsrc-for:0601
    20 schema:author Nfbbf0b964d914900abb95dfa0f7791b4
    21 schema:citation sg:pub.10.1038/336684a0
    22 sg:pub.10.1038/336688a0
    23 sg:pub.10.1038/74447
    24 sg:pub.10.1038/ng1297-498
    25 https://doi.org/10.1006/dbio.2000.9912
    26 https://doi.org/10.1016/0012-1606(83)90183-5
    27 https://doi.org/10.1016/0092-8674(92)90115-s
    28 https://doi.org/10.1021/bi00267a025
    29 https://doi.org/10.1083/jcb.115.3.843
    30 https://doi.org/10.1126/science.279.5349.349
    31 https://doi.org/10.1126/science.282.5391.1145
    32 https://doi.org/10.1126/science.7605428
    33 https://doi.org/10.1146/annurev.cb.02.110186.000331
    34 https://doi.org/10.1172/jci112887
    35 https://doi.org/10.3109/15419069309095680
    36 schema:datePublished 2001-10
    37 schema:datePublishedReg 2001-10-01
    38 schema:description Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least 130 population doublings. In this system, hES cells are cultured on Matrigel or laminin in medium conditioned by MEF. The hES cells maintained on feeders or off feeders express integrin alpha6 and beta1, which may form a laminin-specific receptor. The hES cell populations in feeder-free conditions maintained a normal karyotype, stable proliferation rate, and high telomerase activity. Similar to cells cultured on feeders, hES cells maintained under feeder-free conditions expressed OCT-4, hTERT, alkaline phosphatase, and surface markers including SSEA-4, Tra 1-60, and Tra 1-81. In addition, hES cells maintained without direct feeder contact formed teratomas in SCID/beige mice and differentiated in vitro into cells from all three germ layers. Thus, the cells retain fundamental characteristics of hES cells in this culture system and are suitable for scaleup production.
    39 schema:genre research_article
    40 schema:inLanguage en
    41 schema:isAccessibleForFree false
    42 schema:isPartOf N447374e8625241b4b27aa1d38bed98c0
    43 N4b6aff9094354bd58a71c8cb6affec98
    44 sg:journal.1115214
    45 schema:name Feeder-free growth of undifferentiated human embryonic stem cells
    46 schema:pagination 971-974
    47 schema:productId N1aa107f759df43e5a4edc280a9e7eba3
    48 N3c31b1e87ef64b8b8d7c5640fab99632
    49 N4aaf89e2326f4106bcdf86996d9a2002
    50 N6d71a280f25c4e73bd73087392d4b0f3
    51 Nc49b03761d2e4f4ba0ed86e39ec26b1b
    52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014515248
    53 https://doi.org/10.1038/nbt1001-971
    54 schema:sdDatePublished 2019-04-11T12:13
    55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    56 schema:sdPublisher N940dded3ca5d4565bcca1b4444b52f7a
    57 schema:url http://www.nature.com/articles/nbt1001-971
    58 sgo:license sg:explorer/license/
    59 sgo:sdDataset articles
    60 rdf:type schema:ScholarlyArticle
    61 N04eccc5f075e4b6699516828a6398b18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    62 schema:name Cell Differentiation
    63 rdf:type schema:DefinedTerm
    64 N051aeba1135d418fa33a128d71ea398f rdf:first sg:person.0726523201.14
    65 rdf:rest N186ed20d2624400b9049fcf5b58e033e
    66 N0e82f4e8e847460db54ccabb99582528 rdf:first sg:person.01061360765.53
    67 rdf:rest N57ce1dc0a37b410b8add891d91fb6243
    68 N10ab1e5a268c45d59e2683092f5574a2 schema:affiliation https://www.grid.ac/institutes/grid.418219.7
    69 schema:familyName Golds
    70 schema:givenName Kathaleen
    71 rdf:type schema:Person
    72 N16beb1caffd943d4a5b64b6534c42f62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    73 schema:name Cell Division
    74 rdf:type schema:DefinedTerm
    75 N186ed20d2624400b9049fcf5b58e033e rdf:first N10ab1e5a268c45d59e2683092f5574a2
    76 rdf:rest N0e82f4e8e847460db54ccabb99582528
    77 N1aa107f759df43e5a4edc280a9e7eba3 schema:name readcube_id
    78 schema:value 4526360f7d7ed5ae0cde25e497712ca1157d4fea60a759f826a47bf956e49c2b
    79 rdf:type schema:PropertyValue
    80 N320786f55fef403fab9ccc1911ada2fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    81 schema:name Stage-Specific Embryonic Antigens
    82 rdf:type schema:DefinedTerm
    83 N324fb7f7c6b94a82b02ab4c30c3da9ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Embryo, Nonmammalian
    85 rdf:type schema:DefinedTerm
    86 N39a5649d8f404eacbdd24e35cb9d68d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Reverse Transcriptase Polymerase Chain Reaction
    88 rdf:type schema:DefinedTerm
    89 N3c31b1e87ef64b8b8d7c5640fab99632 schema:name pubmed_id
    90 schema:value 11581665
    91 rdf:type schema:PropertyValue
    92 N447374e8625241b4b27aa1d38bed98c0 schema:volumeNumber 19
    93 rdf:type schema:PublicationVolume
    94 N4aaf89e2326f4106bcdf86996d9a2002 schema:name nlm_unique_id
    95 schema:value 9604648
    96 rdf:type schema:PropertyValue
    97 N4b6aff9094354bd58a71c8cb6affec98 schema:issueNumber 10
    98 rdf:type schema:PublicationIssue
    99 N5628a58c2a644a90a236bbd7bfd98d66 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Culture Media, Conditioned
    101 rdf:type schema:DefinedTerm
    102 N57ce1dc0a37b410b8add891d91fb6243 rdf:first sg:person.0630535243.69
    103 rdf:rest Nb987aec3e2d34d8a86a79ef70c60c8c9
    104 N65fc0a6b8322489e84869473b4de0817 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Glycosphingolipids
    106 rdf:type schema:DefinedTerm
    107 N692d34274884432abad13b10f7a0b260 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Drug Combinations
    109 rdf:type schema:DefinedTerm
    110 N6d71a280f25c4e73bd73087392d4b0f3 schema:name dimensions_id
    111 schema:value pub.1014515248
    112 rdf:type schema:PropertyValue
    113 N70abdb7b23a949fabf3a6fb686422cb8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Cell Culture Techniques
    115 rdf:type schema:DefinedTerm
    116 N940dded3ca5d4565bcca1b4444b52f7a schema:name Springer Nature - SN SciGraph project
    117 rdf:type schema:Organization
    118 N9bd3460c0a5141a18e5262cab961422d rdf:first sg:person.01022674377.31
    119 rdf:rest N051aeba1135d418fa33a128d71ea398f
    120 Nad873a62f7774c2e8b00446f370cd4b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Stem Cells
    122 rdf:type schema:DefinedTerm
    123 Nb823f1ed5e2d499eaa25bf92ea379279 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Collagen
    125 rdf:type schema:DefinedTerm
    126 Nb987aec3e2d34d8a86a79ef70c60c8c9 rdf:first sg:person.01254176236.61
    127 rdf:rest rdf:nil
    128 Nc49b03761d2e4f4ba0ed86e39ec26b1b schema:name doi
    129 schema:value 10.1038/nbt1001-971
    130 rdf:type schema:PropertyValue
    131 Nc6ceeb9b956c40bab7c1b3982b3b9a04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Laminin
    133 rdf:type schema:DefinedTerm
    134 Nc7c081bc6caf44c0ae0593f1d01df7d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Culture Media
    136 rdf:type schema:DefinedTerm
    137 Nc95e9d728c5244e6aa2c23fe3ce750ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Flow Cytometry
    139 rdf:type schema:DefinedTerm
    140 Ncea7e7ae581b4a1ab3ec9e7aa77fa9f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Embryo, Mammalian
    142 rdf:type schema:DefinedTerm
    143 Nd79b02fe80bf496887f7d0425534aa47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Coculture Techniques
    145 rdf:type schema:DefinedTerm
    146 Nfbbf0b964d914900abb95dfa0f7791b4 rdf:first sg:person.01207330163.59
    147 rdf:rest N9bd3460c0a5141a18e5262cab961422d
    148 Nfc6314e4299c4d7fbf4e7b64fc3aa78c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Proteoglycans
    150 rdf:type schema:DefinedTerm
    151 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    152 schema:name Biological Sciences
    153 rdf:type schema:DefinedTerm
    154 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    155 schema:name Biochemistry and Cell Biology
    156 rdf:type schema:DefinedTerm
    157 sg:journal.1115214 schema:issn 1087-0156
    158 1546-1696
    159 schema:name Nature Biotechnology
    160 rdf:type schema:Periodical
    161 sg:person.01022674377.31 schema:affiliation https://www.grid.ac/institutes/grid.418219.7
    162 schema:familyName Inokuma
    163 schema:givenName Margaret S.
    164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022674377.31
    165 rdf:type schema:Person
    166 sg:person.01061360765.53 schema:affiliation https://www.grid.ac/institutes/grid.418219.7
    167 schema:familyName Kundu
    168 schema:givenName Pratima
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061360765.53
    170 rdf:type schema:Person
    171 sg:person.01207330163.59 schema:affiliation https://www.grid.ac/institutes/grid.418219.7
    172 schema:familyName Xu
    173 schema:givenName Chunhui
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207330163.59
    175 rdf:type schema:Person
    176 sg:person.01254176236.61 schema:affiliation https://www.grid.ac/institutes/grid.418219.7
    177 schema:familyName Carpenter
    178 schema:givenName Melissa K.
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254176236.61
    180 rdf:type schema:Person
    181 sg:person.0630535243.69 schema:affiliation https://www.grid.ac/institutes/grid.418219.7
    182 schema:familyName Gold
    183 schema:givenName Joseph D.
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630535243.69
    185 rdf:type schema:Person
    186 sg:person.0726523201.14 schema:affiliation https://www.grid.ac/institutes/grid.418219.7
    187 schema:familyName Denham
    188 schema:givenName Jerrod
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726523201.14
    190 rdf:type schema:Person
    191 sg:pub.10.1038/336684a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038677733
    192 https://doi.org/10.1038/336684a0
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1038/336688a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010804374
    195 https://doi.org/10.1038/336688a0
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1038/74447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033604670
    198 https://doi.org/10.1038/74447
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1038/ng1297-498 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028381516
    201 https://doi.org/10.1038/ng1297-498
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1006/dbio.2000.9912 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053693663
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1016/0012-1606(83)90183-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010044484
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1016/0092-8674(92)90115-s schema:sameAs https://app.dimensions.ai/details/publication/pub.1007440282
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1021/bi00267a025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055167840
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1083/jcb.115.3.843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046025583
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1126/science.279.5349.349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051728512
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1126/science.7605428 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062648139
    218 rdf:type schema:CreativeWork
    219 https://doi.org/10.1146/annurev.cb.02.110186.000331 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039638042
    220 rdf:type schema:CreativeWork
    221 https://doi.org/10.1172/jci112887 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049043498
    222 rdf:type schema:CreativeWork
    223 https://doi.org/10.3109/15419069309095680 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013747112
    224 rdf:type schema:CreativeWork
    225 https://www.grid.ac/institutes/grid.418219.7 schema:alternateName Geron (United States)
    226 schema:name Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025.
    227 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...