Feeder-free growth of undifferentiated human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-10

AUTHORS

Chunhui Xu, Margaret S. Inokuma, Jerrod Denham, Kathaleen Golds, Pratima Kundu, Joseph D. Gold, Melissa K. Carpenter

ABSTRACT

Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least 130 population doublings. In this system, hES cells are cultured on Matrigel or laminin in medium conditioned by MEF. The hES cells maintained on feeders or off feeders express integrin alpha6 and beta1, which may form a laminin-specific receptor. The hES cell populations in feeder-free conditions maintained a normal karyotype, stable proliferation rate, and high telomerase activity. Similar to cells cultured on feeders, hES cells maintained under feeder-free conditions expressed OCT-4, hTERT, alkaline phosphatase, and surface markers including SSEA-4, Tra 1-60, and Tra 1-81. In addition, hES cells maintained without direct feeder contact formed teratomas in SCID/beige mice and differentiated in vitro into cells from all three germ layers. Thus, the cells retain fundamental characteristics of hES cells in this culture system and are suitable for scaleup production. More... »

PAGES

971-974

Journal

TITLE

Nature Biotechnology

ISSUE

10

VOLUME

19

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1001-971

    DOI

    http://dx.doi.org/10.1038/nbt1001-971

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014515248

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11581665


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