Ontology type: schema:ScholarlyArticle Open Access: True
2001-10
AUTHORSDavid K. Han, Jimmy Eng, Huilin Zhou, Ruedi Aebersold
ABSTRACTAn approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins with isotope-coded affinity tag (ICAT) reagents followed by proteolysis of the combined labeled protein samples; and (3) isolation, identification, and quantification of the tagged peptides by multidimensional chromatography, automated tandem mass spectrometry, and computational analysis of the obtained data. The method was used to identify and determine the ratios of abundance of each of 491 proteins contained in the microsomal fractions of naïve and in vitro– differentiated human myeloid leukemia (HL-60) cells. The method and the new software tools to support it are well suited to the large-scale, quantitative analysis of membrane proteins and other classes of proteins that have been refractory to standard proteomics technology. More... »
PAGES946-951
http://scigraph.springernature.com/pub.10.1038/nbt1001-946
DOIhttp://dx.doi.org/10.1038/nbt1001-946
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1029399357
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/11581660
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Chemical Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Analytical Chemistry",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Affinity Labels",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amino Acid Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Differentiation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "HL-60 Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mass Spectrometry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Microsomes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Software",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tetradecanoylphorbol Acetate",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Connecticut Health Center, 263 Farmington Ave., 06030-0002, Farmington, CT",
"id": "http://www.grid.ac/institutes/grid.208078.5",
"name": [
"University of Connecticut Health Center, 263 Farmington Ave., 06030-0002, Farmington, CT"
],
"type": "Organization"
},
"familyName": "Han",
"givenName": "David K.",
"id": "sg:person.0760336115.03",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760336115.03"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute for Systems Biology, 4225 Roosevelt Way NE, 98105-6099, Seattle, WA",
"id": "http://www.grid.ac/institutes/grid.64212.33",
"name": [
"Institute for Systems Biology, 4225 Roosevelt Way NE, 98105-6099, Seattle, WA"
],
"type": "Organization"
},
"familyName": "Eng",
"givenName": "Jimmy",
"id": "sg:person.010623237677.28",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010623237677.28"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute for Systems Biology, 4225 Roosevelt Way NE, 98105-6099, Seattle, WA",
"id": "http://www.grid.ac/institutes/grid.64212.33",
"name": [
"Institute for Systems Biology, 4225 Roosevelt Way NE, 98105-6099, Seattle, WA"
],
"type": "Organization"
},
"familyName": "Zhou",
"givenName": "Huilin",
"id": "sg:person.0720463627.62",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720463627.62"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute for Systems Biology, 4225 Roosevelt Way NE, 98105-6099, Seattle, WA",
"id": "http://www.grid.ac/institutes/grid.64212.33",
"name": [
"Institute for Systems Biology, 4225 Roosevelt Way NE, 98105-6099, Seattle, WA"
],
"type": "Organization"
},
"familyName": "Aebersold",
"givenName": "Ruedi",
"id": "sg:person.015313356237.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015313356237.76"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/86777",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003596509",
"https://doi.org/10.1038/86777"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1016/1044-0305(94)80016-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018629105",
"https://doi.org/10.1016/1044-0305(94)80016-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/75603",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017473553",
"https://doi.org/10.1038/75603"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/8531",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007589470",
"https://doi.org/10.1038/8531"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/343425a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047196635",
"https://doi.org/10.1038/343425a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/10890",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012772847",
"https://doi.org/10.1038/10890"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/13690",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029022565",
"https://doi.org/10.1038/13690"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/bcr276",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002682601",
"https://doi.org/10.1186/bcr276"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/74325",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041828727",
"https://doi.org/10.1038/74325"
],
"type": "CreativeWork"
}
],
"datePublished": "2001-10",
"datePublishedReg": "2001-10-01",
"description": "An approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins with isotope-coded affinity tag (ICAT) reagents followed by proteolysis of the combined labeled protein samples; and (3) isolation, identification, and quantification of the tagged peptides by multidimensional chromatography, automated tandem mass spectrometry, and computational analysis of the obtained data. The method was used to identify and determine the ratios of abundance of each of 491 proteins contained in the microsomal fractions of na\u00efve and in vitro\u2013 differentiated human myeloid leukemia (HL-60) cells. The method and the new software tools to support it are well suited to the large-scale, quantitative analysis of membrane proteins and other classes of proteins that have been refractory to standard proteomics technology.",
"genre": "article",
"id": "sg:pub.10.1038/nbt1001-946",
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.2631587",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2538062",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1115214",
"issn": [
"1087-0156",
"1546-1696"
],
"name": "Nature Biotechnology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "10",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "19"
}
],
"keywords": [
"isotope-coded affinity tag (ICAT) reagents",
"mass spectrometry",
"isotope-coded affinity tags",
"affinity tag reagents",
"class of proteins",
"human myeloid leukemia cells",
"tandem mass spectrometry",
"ratio of abundance",
"multidimensional chromatography",
"microsomal fraction",
"membrane proteins",
"covalent tagging",
"proteomic technologies",
"affinity tag",
"quantitative profiling",
"protein",
"protein samples",
"myeloid leukemia cells",
"systematic identification",
"spectrometry",
"leukemia cells",
"computational analysis",
"cells",
"microsomal protein",
"reagents",
"proteolysis",
"abundance",
"identification",
"chromatography",
"profiling",
"quantitative analysis",
"preparation",
"quantification",
"tags",
"peptides",
"fraction",
"tagging",
"isolation",
"new software tool",
"tissue",
"different states",
"analysis",
"method",
"step",
"samples",
"ratio",
"na\u00efve",
"state",
"software tools",
"tool",
"class",
"data",
"technology",
"approach"
],
"name": "Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry",
"pagination": "946-951",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1029399357"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/nbt1001-946"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"11581660"
]
}
],
"sameAs": [
"https://doi.org/10.1038/nbt1001-946",
"https://app.dimensions.ai/details/publication/pub.1029399357"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:52",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_316.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/nbt1001-946"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1001-946'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1001-946'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1001-946'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1001-946'
This table displays all metadata directly associated to this object as RDF triples.
231 TRIPLES
21 PREDICATES
102 URIs
83 LITERALS
18 BLANK NODES