Integration and expression of the high-molecular-weight glutenin subunit 1Ax1 gene into wheat View Full Text


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Article Info

DATE

1996-09

AUTHORS

F Altpeter, V Vasil, V Srivastava, I K Vasil

ABSTRACT

The unique bread-making characteristic of wheat flour is closely related to the elasticity and extensibility of the gluten proteins stored in the starchy endosperm, particularly the high-molecular-weight glutenin subunits (HMW-GS), which are important in determining gluten and dough elasticity. The quality of wheat cultivars depends on the number and composition of the HMW-GS present. We have introduced the HMW-GS 1Ax1 gene, known to be associated with good bread-making quality, into the Bob White cultivar of wheat (Triticum aestivum L.), in which it is not present in nature, by the biolistic bombardment of cultured immature embryos. Of the 21 independent transformed lines selected, 20 expressed the selectable bar gene, and nine the 1Ax1 gene. The amount of HMW-GS 1Ax1 protein produced in the different transgenic lines varied from 0.6% to 2.3% of the total protein, resulting in an increase of up to 71% in total HMW-GS proteins. The transgenic plants were normal, fertile, and showed Mendelian segregation of the transgenes. The accumulation of HMW-GS 1Ax1 was consistent and stable up to the R3 seed generation. These results demonstrate that it is possible to manipulate both the quantity and quality of HMW-GS, which influence the bread-making quality of wheat. More... »

PAGES

1155

References to SciGraph publications

  • 1993-12. Rapid Production of Transgenic Wheat Plants by Direct Bombardment of Cultured Immature Embryos in NATURE BIOTECHNOLOGY
  • 1995-11. Biotechnology of Breadmaking: Unraveling and Manipulating the Multi-Protein Gluten Complex in BIO/TECHNOLOGY
  • 1992-01. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality in THEORETICAL AND APPLIED GENETICS
  • 1994-09. Molecular improvement of cereals in PLANT MOLECULAR BIOLOGY
  • 1996-07. Expression of a novel high-molecular-weight glutenin subunit gene in transgenic wheat in NATURE BIOTECHNOLOGY
  • 1996-06. Giant proteins with flour power in NATURE
  • 1990-05. Bialaphos selection of stable transformants from maize cell culture in THEORETICAL AND APPLIED GENETICS
  • 1993-07. Enhanced GUS gene expression in cereal/grass cell suspensions and immature embryos using the maize uhiquitin-based plasmid pAHC25 in PLANT CELL REPORTS
  • 1996-05. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants in TRANSGENIC RESEARCH
  • 1979-05. Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree in THEORETICAL AND APPLIED GENETICS
  • 1996-11. Accelerated production of transgenic wheat (Triticum aestivum L.) plants in PLANT CELL REPORTS
  • 1991-02. Separation and quantitative determination of high-molecular-weight subunits of glutenin from different wheat varieties and genetic variants of the variety Sicco in EUROPEAN FOOD RESEARCH AND TECHNOLOGY
  • 1992-01. Segregation of transgenes in maize in PLANT MOLECULAR BIOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 1989-05. Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny in PLANT MOLECULAR BIOLOGY REPORTER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0996-1155

    DOI

    http://dx.doi.org/10.1038/nbt0996-1155

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1021705516

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9631070


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