A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-09

AUTHORS

Robert D. Hall, Tjitske Riksen-Bruinsma, Guy J. Weyens, Inge J. Rosquin, Pascale N. Denys, Ian J. Evans, Jean E. Lathouwers, Marc P. Lefèbvre, Jim M. Dun well, Arjen van Tunen, Frans A. Krens

ABSTRACT

An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L.). A polyethylene glycol-mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium–based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance. More... »

PAGES

1133-1138

References to SciGraph publications

  • 1993-04. Improvement of protoplast culture protocols for Beta vulgaris L. (sugar beet) in PLANT CELL REPORTS
  • 1993-06. Selection of transformed protoplast-derived Zea mays colonies with phosphinothricin and a novel assay using the pH indicator chlorophenol red in PLANTA
  • 1992-10. Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana in PLANTA
  • 1989-02. Stable transformation of sugarbeet protoplasts by electroporation in PLANT CELL REPORTS
  • 1990-05. Transfer of cytoplasm from newBeta CMS sources to sugar beet by asymmetric fusion in THEORETICAL AND APPLIED GENETICS
  • 1993. History of the crop in THE SUGAR BEET CROP
  • 1992-03-01. Transformation of Sugarbeet (Beta vulgaris L.) and Evaluation of Herbicide Resistance in Transgenic Plants in NATURE BIOTECHNOLOGY
  • 1982-03. In vitro transformation of plant protoplasts with Ti-plasmid DNA in NATURE
  • 1994-12. Stomatal patchiness in BIOLOGIA PLANTARUM
  • 1995-11. The selection mechanism of phosphinothricin is influenced by the metabolic status of the tissue in PLANTA
  • 1995-02. Studies of the mechanism of transgene integration into plant protoplasts: improvement of the transformation rate in EUPHYTICA
  • 1988-12. Transformation and regeneration in sugar beet (Beta vulgaris L.) induced by ‘shooter’ mutants of Agrobacterium tumefaciens in EUPHYTICA
  • 1987-10. Reproduction of the beet cyst nematode Heterodera schachtii Schm. on transformed root cultures of Beta vulgaris L. in PLANT CELL REPORTS
  • 1993. The Sugar Beet Crop in NONE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0996-1133

    DOI

    http://dx.doi.org/10.1038/nbt0996-1133

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1012331810

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9631066


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