A strategy for rapid and efficient DNA sequencing by mass spectrometry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-09

AUTHORS

Hubert Köster, Kai Tang, Dong-Jing Fu, Andreas Braun, Dirk van den Boom, Cassandra L. Smith, Robert J. Cotter, Charles R. Cantor

ABSTRACT

Two methods of solid-phase Sanger DNA sequencing followed by detection with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry are demonstrated. In one method, sequencing ladders generated on an immobilized synthetic template were resolved up to the 63-mer including the primer. Detection sensivity and resolution were sufficient for sequence analysis in the given range. This approach is particularly suitable for comparative (diagnostic) DNA sequencing. A second method that has the potential for high throughput de novo DNA sequencing is also presented; it uses immobilized duplex probes with five-base single-stranded overhangs to capture an unknown DNA template serving as primers for Sanger DNA sequencing. The power of mass spectrometry is demonstrated not only by its very high speed, but also by its ability to identify sequences that are not readable using gel electrophoresis. More... »

PAGES

1123-1128

References to SciGraph publications

Journal

TITLE

Nature Biotechnology

ISSUE

9

VOLUME

14

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0996-1123

    DOI

    http://dx.doi.org/10.1038/nbt0996-1123

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1050064454

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9631064


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