Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human Bone Marrow Cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-09

AUTHORS

Peter W. Zandstra, Connie J. Eaves, James M. Piret

ABSTRACT

We have investigated the potential of stirred suspension cultures to support hematopoiesis from starting innocula of normal human bone marrow cells. Initial studies showed that the short-term maintenance of both colony-forming cell (CFC) numbers and their precursors, detected as long-term culture-initiating cells (LTC-IC), could be achieved as well in stirred suspension cultures as in static cultures. Neither of these progenitor cell populations was affected in either type of culture when porous microcarriers were added to provide an increased surface for adherent cell attachment. Supplementation of the medium with 10 ng/ml of Steel factor (SF) and 2 ng/ml of interleukin-3 (IL-3) resulted in a significant expansion of LTC-IC, CFC and total cell numbers in stirred cultures. Both the duration and ultimate magnitude of these expansions were correlated with the initial cell density and after 4 weeks the number of LTC-IC and CFC present in stirred cultures initiated with the highest starting cell concentration tested reflected average increases of 7- and 22-fold, respectively, above input values. Stirred suspension cultures offer the combined advantages of homogeneity and lack of dependence on the formation and maintenance of an adherent cell layer. Our results suggest their applicability to the development of scaled-up bioreactor systems for clinical procedures requiring the production of primitive hematopoietic cell populations. In addition, stirred suspension cultures may offer a new tool for the analysis of hematopoietic regulatory mechanisms. More... »

PAGES

909

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0994-909

DOI

http://dx.doi.org/10.1038/nbt0994-909

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022671474

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7521647


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Marrow Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Division", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colony-Forming Units Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytokines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hematopoietic Cell Growth Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hematopoietic Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interleukin-3", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microspheres", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cell Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Surface Properties", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of British Columbia", 
          "id": "https://www.grid.ac/institutes/grid.17091.3e", 
          "name": [
            "Biotechnology Laboratory & Department of Chemical Engineering, University of British Columbia, Vancouver, B.C., V6T 1Z3, Canada."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zandstra", 
        "givenName": "Peter W.", 
        "id": "sg:person.01070470331.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070470331.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "BC Cancer Agency", 
          "id": "https://www.grid.ac/institutes/grid.248762.d", 
          "name": [
            "Department of Medical Genetics, University of British Columbia, Vancouver, B.C., V6T 1Z3, Canada.", 
            "Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, B.C., V5Z 1L3, Canada."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Eaves", 
        "givenName": "Connie J.", 
        "id": "sg:person.01034353664.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034353664.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of British Columbia", 
          "id": "https://www.grid.ac/institutes/grid.17091.3e", 
          "name": [
            "Biotechnology Laboratory & Department of Chemical Engineering, University of British Columbia, Vancouver, B.C., V6T 1Z3, Canada."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Piret", 
        "givenName": "James M.", 
        "id": "sg:person.01150602264.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150602264.78"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.87.9.3584", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002564071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jss.400130410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002929680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jss.400130410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002929680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.85.23.9253", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005268783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0950-3536(05)80006-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009968004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-71562-4_14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015262893", 
          "https://doi.org/10.1007/978-3-642-71562-4_14"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01666132", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015549207", 
          "https://doi.org/10.1007/bf01666132"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01666132", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015549207", 
          "https://doi.org/10.1007/bf01666132"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm198906223202504", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019220425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp00020a600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022680066"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci114795", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028453037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.19.7512", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028454354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.15.6760", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029680938"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1040910303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036776047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0393-358", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040225469", 
          "https://doi.org/10.1038/nbt0393-358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci115320", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042102522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(90)90304-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042323242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.77.5.2927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047299727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.13.6192", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047554042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1041470221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050277199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.178.3.787", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052691164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075868625", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075950278", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075985707", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075985762", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076036821", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077322165", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077330366", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077401211", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077837297", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079676712", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080096678", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080294617", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081671602", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081707926", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082089082", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082463538", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082464414", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082759788", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082832906", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1994-09", 
    "datePublishedReg": "1994-09-01", 
    "description": "We have investigated the potential of stirred suspension cultures to support hematopoiesis from starting innocula of normal human bone marrow cells. Initial studies showed that the short-term maintenance of both colony-forming cell (CFC) numbers and their precursors, detected as long-term culture-initiating cells (LTC-IC), could be achieved as well in stirred suspension cultures as in static cultures. Neither of these progenitor cell populations was affected in either type of culture when porous microcarriers were added to provide an increased surface for adherent cell attachment. Supplementation of the medium with 10 ng/ml of Steel factor (SF) and 2 ng/ml of interleukin-3 (IL-3) resulted in a significant expansion of LTC-IC, CFC and total cell numbers in stirred cultures. Both the duration and ultimate magnitude of these expansions were correlated with the initial cell density and after 4 weeks the number of LTC-IC and CFC present in stirred cultures initiated with the highest starting cell concentration tested reflected average increases of 7- and 22-fold, respectively, above input values. Stirred suspension cultures offer the combined advantages of homogeneity and lack of dependence on the formation and maintenance of an adherent cell layer. Our results suggest their applicability to the development of scaled-up bioreactor systems for clinical procedures requiring the production of primitive hematopoietic cell populations. In addition, stirred suspension cultures may offer a new tool for the analysis of hematopoietic regulatory mechanisms.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nbt0994-909", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1094195", 
        "issn": [
          "0733-222X"
        ], 
        "name": "Bio/Technology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human Bone Marrow Cells", 
    "pagination": "909", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "6c0268ba094137b364fb63d86486b95e7c57f97b4d6d84cd260da3921e14643f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "7521647"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8309273"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nbt0994-909"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1022671474"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nbt0994-909", 
      "https://app.dimensions.ai/details/publication/pub.1022671474"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:47", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000442.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nbt0994-909"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0994-909'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0994-909'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0994-909'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0994-909'


 

This table displays all metadata directly associated to this object as RDF triples.

252 TRIPLES      21 PREDICATES      84 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nbt0994-909 schema:about N06e3075471cf42eba4366a89e28fce54
2 N25927ccdadb44047b5dc502a5823d526
3 N26d74f5e00a34655adc05bd171ebaa7d
4 N2cafc194fb6a4aa1bd878c556a3830db
5 N3793e5969921498ba07b8664e684ea89
6 N38c2957fe93b42f5ba73f2c77e9da46a
7 N41b6571ba70e42099397e5742fa902a1
8 N59a0d9970e364a4fafb96db20923c605
9 N5ce3ce0fe44646d9b712c0c121f9af8b
10 N71329731022549f8be20207a7d34c35a
11 N7e86267b5b4e4500a9a3929e23c8fab1
12 N9943133ef6064c15834736a1b9d94d1d
13 Nc88d9cf55fe54b43819353c88338fc4c
14 Nce5a6cb9547447bc85b26edb0dabe4c6
15 Nd7db9f4e04f245bba953d78a639b7c97
16 Nd8110b3f10ee465f853daca568bf0878
17 Nfd94b72e31f94f36a01f5748970e4b2f
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author Nc490df553dc342cb82c23ec3aab87bb9
21 schema:citation sg:pub.10.1007/978-3-642-71562-4_14
22 sg:pub.10.1007/bf01666132
23 sg:pub.10.1038/nbt0393-358
24 https://app.dimensions.ai/details/publication/pub.1075868625
25 https://app.dimensions.ai/details/publication/pub.1075950278
26 https://app.dimensions.ai/details/publication/pub.1075985707
27 https://app.dimensions.ai/details/publication/pub.1075985762
28 https://app.dimensions.ai/details/publication/pub.1076036821
29 https://app.dimensions.ai/details/publication/pub.1077322165
30 https://app.dimensions.ai/details/publication/pub.1077330366
31 https://app.dimensions.ai/details/publication/pub.1077401211
32 https://app.dimensions.ai/details/publication/pub.1077837297
33 https://app.dimensions.ai/details/publication/pub.1079676712
34 https://app.dimensions.ai/details/publication/pub.1080096678
35 https://app.dimensions.ai/details/publication/pub.1080294617
36 https://app.dimensions.ai/details/publication/pub.1081671602
37 https://app.dimensions.ai/details/publication/pub.1081707926
38 https://app.dimensions.ai/details/publication/pub.1082089082
39 https://app.dimensions.ai/details/publication/pub.1082463538
40 https://app.dimensions.ai/details/publication/pub.1082464414
41 https://app.dimensions.ai/details/publication/pub.1082759788
42 https://app.dimensions.ai/details/publication/pub.1082832906
43 https://doi.org/10.1002/jcp.1040910303
44 https://doi.org/10.1002/jcp.1041470221
45 https://doi.org/10.1002/jss.400130410
46 https://doi.org/10.1016/0092-8674(90)90304-w
47 https://doi.org/10.1016/s0950-3536(05)80006-x
48 https://doi.org/10.1021/bp00020a600
49 https://doi.org/10.1056/nejm198906223202504
50 https://doi.org/10.1073/pnas.77.5.2927
51 https://doi.org/10.1073/pnas.85.23.9253
52 https://doi.org/10.1073/pnas.87.19.7512
53 https://doi.org/10.1073/pnas.87.9.3584
54 https://doi.org/10.1073/pnas.88.15.6760
55 https://doi.org/10.1073/pnas.89.13.6192
56 https://doi.org/10.1084/jem.178.3.787
57 https://doi.org/10.1172/jci114795
58 https://doi.org/10.1172/jci115320
59 schema:datePublished 1994-09
60 schema:datePublishedReg 1994-09-01
61 schema:description We have investigated the potential of stirred suspension cultures to support hematopoiesis from starting innocula of normal human bone marrow cells. Initial studies showed that the short-term maintenance of both colony-forming cell (CFC) numbers and their precursors, detected as long-term culture-initiating cells (LTC-IC), could be achieved as well in stirred suspension cultures as in static cultures. Neither of these progenitor cell populations was affected in either type of culture when porous microcarriers were added to provide an increased surface for adherent cell attachment. Supplementation of the medium with 10 ng/ml of Steel factor (SF) and 2 ng/ml of interleukin-3 (IL-3) resulted in a significant expansion of LTC-IC, CFC and total cell numbers in stirred cultures. Both the duration and ultimate magnitude of these expansions were correlated with the initial cell density and after 4 weeks the number of LTC-IC and CFC present in stirred cultures initiated with the highest starting cell concentration tested reflected average increases of 7- and 22-fold, respectively, above input values. Stirred suspension cultures offer the combined advantages of homogeneity and lack of dependence on the formation and maintenance of an adherent cell layer. Our results suggest their applicability to the development of scaled-up bioreactor systems for clinical procedures requiring the production of primitive hematopoietic cell populations. In addition, stirred suspension cultures may offer a new tool for the analysis of hematopoietic regulatory mechanisms.
62 schema:genre research_article
63 schema:inLanguage en
64 schema:isAccessibleForFree false
65 schema:isPartOf N1aaf09c59fe24d27a4ce67198f72d04f
66 N60150c3ec20e4ea19ef578eb922915e5
67 sg:journal.1094195
68 schema:name Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human Bone Marrow Cells
69 schema:pagination 909
70 schema:productId N1549ee6b4b7347cabbbc91a3a64993e2
71 N27e2165d33e940da8edfe502220e781e
72 N3d4aadcba3a846ce8f1c768364d55fe2
73 Nc0a369a5b6c64670868f6fd04027cb33
74 Nf0a17b86bd284d6894c6926e7b3339cf
75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022671474
76 https://doi.org/10.1038/nbt0994-909
77 schema:sdDatePublished 2019-04-10T19:47
78 schema:sdLicense https://scigraph.springernature.com/explorer/license/
79 schema:sdPublisher N78df89d99a5248a7a21a5c4beda555ba
80 schema:url https://www.nature.com/articles/nbt0994-909
81 sgo:license sg:explorer/license/
82 sgo:sdDataset articles
83 rdf:type schema:ScholarlyArticle
84 N06e3075471cf42eba4366a89e28fce54 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Stem Cell Factor
86 rdf:type schema:DefinedTerm
87 N1549ee6b4b7347cabbbc91a3a64993e2 schema:name doi
88 schema:value 10.1038/nbt0994-909
89 rdf:type schema:PropertyValue
90 N1aaf09c59fe24d27a4ce67198f72d04f schema:volumeNumber 12
91 rdf:type schema:PublicationVolume
92 N25927ccdadb44047b5dc502a5823d526 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Hematopoietic Stem Cells
94 rdf:type schema:DefinedTerm
95 N26d74f5e00a34655adc05bd171ebaa7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Biotechnology
97 rdf:type schema:DefinedTerm
98 N27e2165d33e940da8edfe502220e781e schema:name pubmed_id
99 schema:value 7521647
100 rdf:type schema:PropertyValue
101 N2cafc194fb6a4aa1bd878c556a3830db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Bone Marrow Cells
103 rdf:type schema:DefinedTerm
104 N3793e5969921498ba07b8664e684ea89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Colony-Forming Units Assay
106 rdf:type schema:DefinedTerm
107 N38c2957fe93b42f5ba73f2c77e9da46a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Interleukin-3
109 rdf:type schema:DefinedTerm
110 N3d4aadcba3a846ce8f1c768364d55fe2 schema:name dimensions_id
111 schema:value pub.1022671474
112 rdf:type schema:PropertyValue
113 N41b6571ba70e42099397e5742fa902a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Surface Properties
115 rdf:type schema:DefinedTerm
116 N59a0d9970e364a4fafb96db20923c605 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Cell Adhesion
118 rdf:type schema:DefinedTerm
119 N5ce3ce0fe44646d9b712c0c121f9af8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Cell Division
121 rdf:type schema:DefinedTerm
122 N60150c3ec20e4ea19ef578eb922915e5 schema:issueNumber 9
123 rdf:type schema:PublicationIssue
124 N71329731022549f8be20207a7d34c35a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Microspheres
126 rdf:type schema:DefinedTerm
127 N78df89d99a5248a7a21a5c4beda555ba schema:name Springer Nature - SN SciGraph project
128 rdf:type schema:Organization
129 N7e86267b5b4e4500a9a3929e23c8fab1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Kinetics
131 rdf:type schema:DefinedTerm
132 N9943133ef6064c15834736a1b9d94d1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Cell Survival
134 rdf:type schema:DefinedTerm
135 Nc0a369a5b6c64670868f6fd04027cb33 schema:name nlm_unique_id
136 schema:value 8309273
137 rdf:type schema:PropertyValue
138 Nc490df553dc342cb82c23ec3aab87bb9 rdf:first sg:person.01070470331.34
139 rdf:rest Nf72347fc44a149a0a550647e5b5d94ea
140 Nc88d9cf55fe54b43819353c88338fc4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Culture Techniques
142 rdf:type schema:DefinedTerm
143 Nce5a6cb9547447bc85b26edb0dabe4c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Hematopoietic Cell Growth Factors
145 rdf:type schema:DefinedTerm
146 Nd7db9f4e04f245bba953d78a639b7c97 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Cytokines
148 rdf:type schema:DefinedTerm
149 Nd8110b3f10ee465f853daca568bf0878 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Time Factors
151 rdf:type schema:DefinedTerm
152 Nef29f084db274cfab67abeb63150529f rdf:first sg:person.01150602264.78
153 rdf:rest rdf:nil
154 Nf0a17b86bd284d6894c6926e7b3339cf schema:name readcube_id
155 schema:value 6c0268ba094137b364fb63d86486b95e7c57f97b4d6d84cd260da3921e14643f
156 rdf:type schema:PropertyValue
157 Nf72347fc44a149a0a550647e5b5d94ea rdf:first sg:person.01034353664.50
158 rdf:rest Nef29f084db274cfab67abeb63150529f
159 Nfd94b72e31f94f36a01f5748970e4b2f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Humans
161 rdf:type schema:DefinedTerm
162 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
163 schema:name Biological Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
166 schema:name Biochemistry and Cell Biology
167 rdf:type schema:DefinedTerm
168 sg:journal.1094195 schema:issn 0733-222X
169 schema:name Bio/Technology
170 rdf:type schema:Periodical
171 sg:person.01034353664.50 schema:affiliation https://www.grid.ac/institutes/grid.248762.d
172 schema:familyName Eaves
173 schema:givenName Connie J.
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034353664.50
175 rdf:type schema:Person
176 sg:person.01070470331.34 schema:affiliation https://www.grid.ac/institutes/grid.17091.3e
177 schema:familyName Zandstra
178 schema:givenName Peter W.
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070470331.34
180 rdf:type schema:Person
181 sg:person.01150602264.78 schema:affiliation https://www.grid.ac/institutes/grid.17091.3e
182 schema:familyName Piret
183 schema:givenName James M.
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150602264.78
185 rdf:type schema:Person
186 sg:pub.10.1007/978-3-642-71562-4_14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015262893
187 https://doi.org/10.1007/978-3-642-71562-4_14
188 rdf:type schema:CreativeWork
189 sg:pub.10.1007/bf01666132 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015549207
190 https://doi.org/10.1007/bf01666132
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/nbt0393-358 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040225469
193 https://doi.org/10.1038/nbt0393-358
194 rdf:type schema:CreativeWork
195 https://app.dimensions.ai/details/publication/pub.1075868625 schema:CreativeWork
196 https://app.dimensions.ai/details/publication/pub.1075950278 schema:CreativeWork
197 https://app.dimensions.ai/details/publication/pub.1075985707 schema:CreativeWork
198 https://app.dimensions.ai/details/publication/pub.1075985762 schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1076036821 schema:CreativeWork
200 https://app.dimensions.ai/details/publication/pub.1077322165 schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1077330366 schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1077401211 schema:CreativeWork
203 https://app.dimensions.ai/details/publication/pub.1077837297 schema:CreativeWork
204 https://app.dimensions.ai/details/publication/pub.1079676712 schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1080096678 schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1080294617 schema:CreativeWork
207 https://app.dimensions.ai/details/publication/pub.1081671602 schema:CreativeWork
208 https://app.dimensions.ai/details/publication/pub.1081707926 schema:CreativeWork
209 https://app.dimensions.ai/details/publication/pub.1082089082 schema:CreativeWork
210 https://app.dimensions.ai/details/publication/pub.1082463538 schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1082464414 schema:CreativeWork
212 https://app.dimensions.ai/details/publication/pub.1082759788 schema:CreativeWork
213 https://app.dimensions.ai/details/publication/pub.1082832906 schema:CreativeWork
214 https://doi.org/10.1002/jcp.1040910303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036776047
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1002/jcp.1041470221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050277199
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1002/jss.400130410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002929680
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/0092-8674(90)90304-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1042323242
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/s0950-3536(05)80006-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009968004
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1021/bp00020a600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022680066
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1056/nejm198906223202504 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019220425
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1073/pnas.77.5.2927 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047299727
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1073/pnas.85.23.9253 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005268783
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1073/pnas.87.19.7512 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028454354
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1073/pnas.87.9.3584 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002564071
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1073/pnas.88.15.6760 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029680938
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1073/pnas.89.13.6192 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047554042
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1084/jem.178.3.787 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052691164
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1172/jci114795 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028453037
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1172/jci115320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042102522
245 rdf:type schema:CreativeWork
246 https://www.grid.ac/institutes/grid.17091.3e schema:alternateName University of British Columbia
247 schema:name Biotechnology Laboratory & Department of Chemical Engineering, University of British Columbia, Vancouver, B.C., V6T 1Z3, Canada.
248 rdf:type schema:Organization
249 https://www.grid.ac/institutes/grid.248762.d schema:alternateName BC Cancer Agency
250 schema:name Department of Medical Genetics, University of British Columbia, Vancouver, B.C., V6T 1Z3, Canada.
251 Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, B.C., V5Z 1L3, Canada.
252 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...