Agrobacterium-Mediated Transformation of Plum (Prunus domestica L.) Hypocotyl Slices and Regeneration of Transgenic Plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-09

AUTHORS

S. Mante, P. H. Morgens, R. Scorza, J. M. Cordts, A. M. Callahan

ABSTRACT

Plum plants were regenerated from hypo-cotyl segments derived from the embryonic axes of ungerminated seeds stored for up to six months at 4°C. Shoots were produced in 45 percent of the explants with an average of 8 shoots per explant. This regeneration system was used for Agrobacterium-mediated transformation with plasmids containing genes for neo-mycin phosphotransferase (NPTII) and β-glucuronidase (GUS). Approximately 25 percent of the explants produced shoots on regeneration medium in the presence of antibiotics, and 30 percent of these could be rooted. Putative transgenic plants expressed NPTII and GUS activity, and Southern analyses demonstrated integration of T-DNA into the plum genome. More... »

PAGES

853

References to SciGraph publications

  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1990-08. Stable transformation of papaya via microprojectile bombardment in PLANT CELL REPORTS
  • 1989-03. Genetic transformation of apple (Malus pumila Mill.) using a disarmed Ti-binary vector in PLANT CELL REPORTS
  • 1990-08. Agrobacterium-mediated transformation of peach (Prunus persica L. batsch) leaf segments, immature embryos, and long-term embryogenic callus in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1990-03. Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter in PLANT MOLECULAR BIOLOGY
  • 1989-11. Regeneration of plants from callus and embryos of ‘Royal’ apricot in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-10. Plant regeneration from cotyledons of Prunus persica, Prunus domestica, and Prunus cerasus in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1987-12. A simplified method for the detection of neomycin phosphotransferase II activity in transformed plant tissues in PLANT MOLECULAR BIOLOGY REPORTER
  • 1990-09. Novel and useful properties of a chimeric plant promoter combining CaMV 35S and MAS elements in PLANT MOLECULAR BIOLOGY
  • 1986. Fruit Trees in TREES I
  • 1987-02. Agrobacterium mediated transformation and regeneration of Populus in MOLECULAR GENETICS AND GENOMICS
  • 1985-06. Regeneration of peach plants from callus derived from immature embryos in THEORETICAL AND APPLIED GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0991-853

    DOI

    http://dx.doi.org/10.1038/nbt0991-853

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1009875736


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