Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-09

AUTHORS

M E Fromm, F Morrish, C Armstrong, R Williams, J Thomas, T M Klein

ABSTRACT

We obtained transgenic maize plants by using high-velocity microprojectiles to transfer genes into embryongenic cells. Two selectable genes were used to confer resistance to either chlorsulfuron or phosphinothricin, and genes encoding either E. coli beta-glucuronidase or firefly luciferase were used as markers to provide convenient assays for transformation. When regenerated without selection, only two of the eight transformed embryogenic calli obtained produced transgenic maize plants. With selection, transgenic plants were obtained from three of the other eight calli. One of the two initial lines produced 15 fertile transgenic plants. The progeny of these plants contained and expressed the foreign genes. Luciferase expression could be visualized, in the presence of added luciferin, by overlaying leaf sections with color film. More... »

PAGES

833

References to SciGraph publications

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  • Journal

    TITLE

    Bio/Technology

    ISSUE

    9

    VOLUME

    8

    Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0990-833

    DOI

    http://dx.doi.org/10.1038/nbt0990-833

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025361066

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1366794


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