Improved DNA: liposome complexes for increased systemic delivery and gene expression View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-07

AUTHORS

Nancy Smyth Templeton, Danilo D. Lasic, Peter M. Frederik, Helmut H. Strey, David D. Roberts, George N. Pavlakis

ABSTRACT

To increase cationic liposome-mediated intravenous DNA delivery extruded DOTAP:cholesterol liposomes were used to form complexes with DNA, resulting in enhanced expression of the chloramphenicol acetyltransferase gene in most tissues examined. The DNA:liposome ratio, and mild sonication, heating, and extrusion steps used for liposome preparation were crucial for improved systemic delivery. Size fractionation studies showed that maximal gene expression was produced by a homogeneous population of DNA:liposome complexes between 200 to 450 nm in size. Cryo-electron microscopy examination demonstrates that the DNA:liposome complexes have a novel morphology, and that the DNA is condensed on the interior of invaginated liposomes between two lipid bilayers. This structure could account for the high efficiency of gene delivery in vivo and for the broad tissue distribution of the DNA:liposome complexes. Ligands can be placed on the outside of this structure to provide for targeted gene delivery. More... »

PAGES

647

Journal

TITLE

Nature Biotechnology

ISSUE

7

VOLUME

15

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0797-647

    DOI

    http://dx.doi.org/10.1038/nbt0797-647

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028041171

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9219267


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