Regeneration of transgenic cassava plants (Manihot esculenta Crantz) from microbombarded embryogenic suspension cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-06

AUTHORS

C Schöpke, N Taylor, R Cárcamo, N K Konan, P Marmey, G G Henshaw, R N Beachy, C Fauquet

ABSTRACT

A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene encoding neomycin phosphotransferase (nptII) and a gene encoding beta-glucuronidase (uidA). Selection of bombarded tissue with paromomycin resulted in the establishment of putative transgenic embryogenic calli. In most of these calli, beta-glucuronidase was detected histochemically. Molecular analysis of paromomycin-resistant embryogenic calli and of plants regenerated from these calli, confirmed the stable integration of bombarded DNA into the cassava genome. More... »

PAGES

731

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0696-731

DOI

http://dx.doi.org/10.1038/nbt0696-731

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007029869

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9630980


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