Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1992-06

AUTHORS

Vimla Vasil, Ana M. Castillo, Michael E. Fromm, Indra K. Vasil

ABSTRACT

We have obtained fertile transgenic wheat plants resistant to the broad spectrum herbicide Basta® (active ingredient phosphinothricin, PPT) by high velocity microprojectile bombardment. The plasmid pBARGUS was used to deliver the selectable bar gene into cells of Type C long–term regenerable embryogenic callus. Phosphinothricin acetyltransferase (PAT) enzyme activity encoded by the bar gene was demonstrated in four independent putative transformed callus lines selected on Basta® from two cultivars. Although somatic embryos and shoots were formed in each of the four lines, plants were recovered only from two. More than 100 green R0 plants were regenerated from the first callus line, of which 40 were grown to maturity. PAT activity was shown in each of the 28 R0 plants tested. Southern analyses confirmed the presence of the bar gene in all of the callus lines, and in each of the R0 and two of the four R1 plants tested. Transformed R0, R1 and R2 plants were resistant to topical applications of Basta®, and the bar gene segregated as a dominant Mendelian trait in R1 and R2 plants. More... »

PAGES

667

References to SciGraph publications

  • 1990-09. Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants in BIO/TECHNOLOGY
  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1988-07. Transient expression of foreign genes in rice, wheat and soybean cells following particle bombardment in PLANT MOLECULAR BIOLOGY
  • 1991-08. Stably Transformed Callus Lines from Microprojectile Bombardment of Cell Suspension Cultures of Wheat in NATURE BIOTECHNOLOGY
  • 1990-05. Bialaphos selection of stable transformants from maize cell culture in THEORETICAL AND APPLIED GENETICS
  • 1990-05. Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures in PLANT CELL REPORTS
  • 1991-10. Production of Transgenic Rice (Oryza Sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos in NATURE BIOTECHNOLOGY
  • 1990-05. Regeneration of Plants from Embryogenic Suspension Culture Protoplasts of Wheat (Triticum aestivum L.) in NATURE BIOTECHNOLOGY
  • 1989-03. Fertile transgenic rice plants regenerated from transformed protoplasts in NATURE
  • 1990-05. Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.) in THEORETICAL AND APPLIED GENETICS
  • 1989-05. Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny in PLANT MOLECULAR BIOLOGY REPORTER
  • Journal

    TITLE

    Nature Biotechnology

    ISSUE

    6

    VOLUME

    10

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    http://scigraph.springernature.com/pub.10.1038/nbt0692-667

    DOI

    http://dx.doi.org/10.1038/nbt0692-667

    DIMENSIONS

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