Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-05

AUTHORS

Huilin Zhou, Jeffrey A. Ranish, Julian D. Watts, Ruedi Aebersold

ABSTRACT

The adaptation of sequences of chemical reactions to a solid-phase format has been essential to the automation, reproducibility, and efficiency of a number of biotechnological processes including peptide and oligonucleotide synthesis and sequencing1,2,3,4. Here we describe a method for the site-specific, stable isotopic labeling of cysteinyl peptides in complex peptide mixtures through a solid-phase capture and release process, and the concomitant isolation of the labeled peptides. The recovered peptides were analyzed by microcapillary liquid chromatography and tandem mass spectrometry (μLC-MS/MS) to determine their sequences and relative quantities. The method was used to detect galactose-induced changes in protein abundance in the yeast Saccharomyces cerevisiae. A side-by-side comparison with the isotope-coded affinity tag (ICAT) method5 demonstrated that the solid-phase method for stable isotope tagging of peptides is comparatively simpler, more efficient, and more sensitive. More... »

PAGES

512-515

Journal

TITLE

Nature Biotechnology

ISSUE

5

VOLUME

20

Related Patents

  • Intermediates Of Active Esters For Peptide Synthesis, Labeling, Analysis; Purity
  • Micelle- And Microemulsion-Assisted Planar Separations Platform For Proteomics
  • Sets And Compositions Pertaining To Analyte Determination
  • Methods Of Quantitation And Identification Of Peptides And Proteins
  • Mass Labels
  • Using Mass Analysis To Monitor Concentration Of Analytes In Fluids; Electrophilic And Chromatographic Separation
  • Analysis Of Mass Spectral Data In The Quiet Zones
  • Active Esters Of N-Substituted Piperazine Acetic Acids, Including Isotopically Enriched Versions Thereof
  • Labeling Biopolymers; Obtain Sample Of Biopolymer, Incubate With Solid Support, Remove Preerential Functional Groups, Detect Signal And Evaluate Sample
  • Contacting With A Solid Support Coupled To A Chemical Comprising A Cleavable Functional Group; Mass Spectrometry Tag Which Transfers To The Polypeptide Upon Cleavage; Allows Quantitative Analysis Of Complex Mixtures Of Analytes
  • Using Mass Tag Complex As Tool In Monitoring Transcriptional Patterns Of A Plurality Of Biological Molecules; Diagnosing Infection, Cell Proliferative, Inflammator And Developmental Disorers
  • Kits Pertaining To Analyte Determination
  • Mass Spectrometric Quantitation Method For Biomolecules Based On Metabolically Labeled Internal Standards
  • Materials And Methods For Controlling Isotope Effects During Fractionation Of Analytes
  • Affinity Capture Of Peptides By Microarray And Related Methods
  • Biotinated Macromolecules Having Protein Reactive Groups That React With Functional Groups On Proteins Including Sulfhydryl And Amine Groups, Used In The Detection And Isolation Of Targets From Heterologous Mixtures
  • Capture Compounds, Collections Thereof And Methods For Analyzing The Proteome And Complex Compositions
  • Capture Compounds, Collections Thereof And Methods For Analyzing The Proteome And Complex Compositions
  • Determining Absolute Quantity As Well As Alternative Splice Forms And Modifications Of Proteins Present In A Biological Sample Rapidly And In An Automated Manner
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0502-512

    DOI

    http://dx.doi.org/10.1038/nbt0502-512

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1023426571

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11981568


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Chemical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Analytical Chemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0305", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Organic Chemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromatography, Liquid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cysteine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gas Chromatography-Mass Spectrometry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Isotopes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mass Spectrometry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Models, Chemical", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Peptides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Saccharomyces cerevisiae", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Time Factors", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhou", 
            "givenName": "Huilin", 
            "id": "sg:person.0720463627.62", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720463627.62"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ranish", 
            "givenName": "Jeffrey A.", 
            "id": "sg:person.010125731727.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010125731727.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Watts", 
            "givenName": "Julian D.", 
            "id": "sg:person.0667127272.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667127272.18"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Aebersold", 
            "givenName": "Ruedi", 
            "id": "sg:person.015313356237.76", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015313356237.76"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/86777", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003596509", 
              "https://doi.org/10.1038/86777"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/85686", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050637706", 
              "https://doi.org/10.1038/85686"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/13690", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029022565", 
              "https://doi.org/10.1038/13690"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/310105a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006345158", 
              "https://doi.org/10.1038/310105a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1001-946", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029399357", 
              "https://doi.org/10.1038/nbt1001-946"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1016/1044-0305(94)80016-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018629105", 
              "https://doi.org/10.1016/1044-0305(94)80016-2"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2002-05", 
        "datePublishedReg": "2002-05-01", 
        "description": "The adaptation of sequences of chemical reactions to a solid-phase format has been essential to the automation, reproducibility, and efficiency of a number of biotechnological processes including peptide and oligonucleotide synthesis and sequencing1,2,3,4. Here we describe a method for the site-specific, stable isotopic labeling of cysteinyl peptides in complex peptide mixtures through a solid-phase capture and release process, and the concomitant isolation of the labeled peptides. The recovered peptides were analyzed by microcapillary liquid chromatography and tandem mass spectrometry (\u03bcLC-MS/MS) to determine their sequences and relative quantities. The method was used to detect galactose-induced changes in protein abundance in the yeast Saccharomyces cerevisiae. A side-by-side comparison with the isotope-coded affinity tag (ICAT) method5 demonstrated that the solid-phase method for stable isotope tagging of peptides is comparatively simpler, more efficient, and more sensitive.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/nbt0502-512", 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2631587", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2381160", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2439911", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "20"
          }
        ], 
        "keywords": [
          "complex peptide mixtures", 
          "isotope tagging", 
          "stable isotope tagging", 
          "solid-phase format", 
          "solid-phase method", 
          "solid-phase capture", 
          "microcapillary liquid chromatography", 
          "cysteinyl peptides", 
          "peptide mixtures", 
          "stable isotopic labeling", 
          "tandem mass", 
          "oligonucleotide synthesis", 
          "chemical reactions", 
          "mass spectrometry", 
          "isotopic labeling", 
          "liquid chromatography", 
          "concomitant isolation", 
          "peptides", 
          "biotechnological processes", 
          "quantitative proteome analysis", 
          "spectrometry", 
          "synthesis", 
          "chromatography", 
          "reaction", 
          "proteome analysis", 
          "relative quantities", 
          "mixture", 
          "reproducibility", 
          "method", 
          "process", 
          "capture", 
          "efficiency", 
          "side comparison", 
          "labeling", 
          "isolation", 
          "tagging", 
          "quantity", 
          "analysis", 
          "sequence", 
          "protein abundance", 
          "comparison", 
          "mass", 
          "abundance", 
          "changes", 
          "side", 
          "format", 
          "number", 
          "automation", 
          "yeast Saccharomyces", 
          "Saccharomyces", 
          "adaptation"
        ], 
        "name": "Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry", 
        "pagination": "512-515", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1023426571"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt0502-512"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "11981568"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt0502-512", 
          "https://app.dimensions.ai/details/publication/pub.1023426571"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-08-04T16:53", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_351.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/nbt0502-512"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0502-512'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0502-512'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0502-512'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0502-512'


     

    This table displays all metadata directly associated to this object as RDF triples.

    207 TRIPLES      21 PREDICATES      94 URIs      79 LITERALS      17 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt0502-512 schema:about N07488a649ba84deab743bbb691178c8e
    2 N0c8eccc6eed0422380d2dd5cf006b23c
    3 N21b23099f2154c73819010c729dc5fb8
    4 N4cf8933d78304173b2db92bf4cc976d9
    5 N59fa563590da4c07b716bd66a1e179d0
    6 N6385a9c7d7b845e9834b4940fbc287a9
    7 N7f0a254fed87477fb56176b66c50027c
    8 N9d62ed9827bf4a48a2f7cd3d6d5f5f31
    9 Nd78e03326453440f8e9ad71633a1a18f
    10 Nea619f5e3718495e9483177ee8055a3f
    11 anzsrc-for:03
    12 anzsrc-for:0301
    13 anzsrc-for:0305
    14 schema:author N7bce5276e0b849bd90f958278574cae9
    15 schema:citation sg:pub.10.1016/1044-0305(94)80016-2
    16 sg:pub.10.1038/13690
    17 sg:pub.10.1038/310105a0
    18 sg:pub.10.1038/85686
    19 sg:pub.10.1038/86777
    20 sg:pub.10.1038/nbt1001-946
    21 schema:datePublished 2002-05
    22 schema:datePublishedReg 2002-05-01
    23 schema:description The adaptation of sequences of chemical reactions to a solid-phase format has been essential to the automation, reproducibility, and efficiency of a number of biotechnological processes including peptide and oligonucleotide synthesis and sequencing1,2,3,4. Here we describe a method for the site-specific, stable isotopic labeling of cysteinyl peptides in complex peptide mixtures through a solid-phase capture and release process, and the concomitant isolation of the labeled peptides. The recovered peptides were analyzed by microcapillary liquid chromatography and tandem mass spectrometry (μLC-MS/MS) to determine their sequences and relative quantities. The method was used to detect galactose-induced changes in protein abundance in the yeast Saccharomyces cerevisiae. A side-by-side comparison with the isotope-coded affinity tag (ICAT) method5 demonstrated that the solid-phase method for stable isotope tagging of peptides is comparatively simpler, more efficient, and more sensitive.
    24 schema:genre article
    25 schema:isAccessibleForFree false
    26 schema:isPartOf N046a371339cc4b66ac718d14b7e8f791
    27 Na540dd99c23d4348aa2595f077c0f322
    28 sg:journal.1115214
    29 schema:keywords Saccharomyces
    30 abundance
    31 adaptation
    32 analysis
    33 automation
    34 biotechnological processes
    35 capture
    36 changes
    37 chemical reactions
    38 chromatography
    39 comparison
    40 complex peptide mixtures
    41 concomitant isolation
    42 cysteinyl peptides
    43 efficiency
    44 format
    45 isolation
    46 isotope tagging
    47 isotopic labeling
    48 labeling
    49 liquid chromatography
    50 mass
    51 mass spectrometry
    52 method
    53 microcapillary liquid chromatography
    54 mixture
    55 number
    56 oligonucleotide synthesis
    57 peptide mixtures
    58 peptides
    59 process
    60 protein abundance
    61 proteome analysis
    62 quantitative proteome analysis
    63 quantity
    64 reaction
    65 relative quantities
    66 reproducibility
    67 sequence
    68 side
    69 side comparison
    70 solid-phase capture
    71 solid-phase format
    72 solid-phase method
    73 spectrometry
    74 stable isotope tagging
    75 stable isotopic labeling
    76 synthesis
    77 tagging
    78 tandem mass
    79 yeast Saccharomyces
    80 schema:name Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry
    81 schema:pagination 512-515
    82 schema:productId N6621a843efd042b883e3e835780a320d
    83 N7858fc45b3794f07b2bd00860bbeefb1
    84 N7e3222a316a94e9ba22849824ad6843b
    85 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023426571
    86 https://doi.org/10.1038/nbt0502-512
    87 schema:sdDatePublished 2022-08-04T16:53
    88 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    89 schema:sdPublisher N99fae14b878144569f29c19754a4f31c
    90 schema:url https://doi.org/10.1038/nbt0502-512
    91 sgo:license sg:explorer/license/
    92 sgo:sdDataset articles
    93 rdf:type schema:ScholarlyArticle
    94 N046a371339cc4b66ac718d14b7e8f791 schema:volumeNumber 20
    95 rdf:type schema:PublicationVolume
    96 N07488a649ba84deab743bbb691178c8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Models, Chemical
    98 rdf:type schema:DefinedTerm
    99 N0c8eccc6eed0422380d2dd5cf006b23c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Cysteine
    101 rdf:type schema:DefinedTerm
    102 N21b23099f2154c73819010c729dc5fb8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Gas Chromatography-Mass Spectrometry
    104 rdf:type schema:DefinedTerm
    105 N2258cb7fa4f941db9c4f60d8784a4d4d rdf:first sg:person.010125731727.05
    106 rdf:rest N8fb7957e8de24c7189fd172a856b0363
    107 N4cf8933d78304173b2db92bf4cc976d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Mass Spectrometry
    109 rdf:type schema:DefinedTerm
    110 N59fa563590da4c07b716bd66a1e179d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Peptides
    112 rdf:type schema:DefinedTerm
    113 N6385a9c7d7b845e9834b4940fbc287a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Saccharomyces cerevisiae
    115 rdf:type schema:DefinedTerm
    116 N6621a843efd042b883e3e835780a320d schema:name dimensions_id
    117 schema:value pub.1023426571
    118 rdf:type schema:PropertyValue
    119 N7858fc45b3794f07b2bd00860bbeefb1 schema:name doi
    120 schema:value 10.1038/nbt0502-512
    121 rdf:type schema:PropertyValue
    122 N7bce5276e0b849bd90f958278574cae9 rdf:first sg:person.0720463627.62
    123 rdf:rest N2258cb7fa4f941db9c4f60d8784a4d4d
    124 N7e3222a316a94e9ba22849824ad6843b schema:name pubmed_id
    125 schema:value 11981568
    126 rdf:type schema:PropertyValue
    127 N7f0a254fed87477fb56176b66c50027c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Isotopes
    129 rdf:type schema:DefinedTerm
    130 N87a7697fd4214e66b9d9599c8a639519 rdf:first sg:person.015313356237.76
    131 rdf:rest rdf:nil
    132 N8fb7957e8de24c7189fd172a856b0363 rdf:first sg:person.0667127272.18
    133 rdf:rest N87a7697fd4214e66b9d9599c8a639519
    134 N99fae14b878144569f29c19754a4f31c schema:name Springer Nature - SN SciGraph project
    135 rdf:type schema:Organization
    136 N9d62ed9827bf4a48a2f7cd3d6d5f5f31 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Proteins
    138 rdf:type schema:DefinedTerm
    139 Na540dd99c23d4348aa2595f077c0f322 schema:issueNumber 5
    140 rdf:type schema:PublicationIssue
    141 Nd78e03326453440f8e9ad71633a1a18f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Chromatography, Liquid
    143 rdf:type schema:DefinedTerm
    144 Nea619f5e3718495e9483177ee8055a3f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    145 schema:name Time Factors
    146 rdf:type schema:DefinedTerm
    147 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
    148 schema:name Chemical Sciences
    149 rdf:type schema:DefinedTerm
    150 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
    151 schema:name Analytical Chemistry
    152 rdf:type schema:DefinedTerm
    153 anzsrc-for:0305 schema:inDefinedTermSet anzsrc-for:
    154 schema:name Organic Chemistry
    155 rdf:type schema:DefinedTerm
    156 sg:grant.2381160 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt0502-512
    157 rdf:type schema:MonetaryGrant
    158 sg:grant.2439911 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt0502-512
    159 rdf:type schema:MonetaryGrant
    160 sg:grant.2631587 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt0502-512
    161 rdf:type schema:MonetaryGrant
    162 sg:journal.1115214 schema:issn 1087-0156
    163 1546-1696
    164 schema:name Nature Biotechnology
    165 schema:publisher Springer Nature
    166 rdf:type schema:Periodical
    167 sg:person.010125731727.05 schema:affiliation grid-institutes:None
    168 schema:familyName Ranish
    169 schema:givenName Jeffrey A.
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010125731727.05
    171 rdf:type schema:Person
    172 sg:person.015313356237.76 schema:affiliation grid-institutes:None
    173 schema:familyName Aebersold
    174 schema:givenName Ruedi
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015313356237.76
    176 rdf:type schema:Person
    177 sg:person.0667127272.18 schema:affiliation grid-institutes:None
    178 schema:familyName Watts
    179 schema:givenName Julian D.
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667127272.18
    181 rdf:type schema:Person
    182 sg:person.0720463627.62 schema:affiliation grid-institutes:None
    183 schema:familyName Zhou
    184 schema:givenName Huilin
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720463627.62
    186 rdf:type schema:Person
    187 sg:pub.10.1016/1044-0305(94)80016-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018629105
    188 https://doi.org/10.1016/1044-0305(94)80016-2
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1038/13690 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029022565
    191 https://doi.org/10.1038/13690
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.1038/310105a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006345158
    194 https://doi.org/10.1038/310105a0
    195 rdf:type schema:CreativeWork
    196 sg:pub.10.1038/85686 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050637706
    197 https://doi.org/10.1038/85686
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1038/86777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003596509
    200 https://doi.org/10.1038/86777
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1038/nbt1001-946 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029399357
    203 https://doi.org/10.1038/nbt1001-946
    204 rdf:type schema:CreativeWork
    205 grid-institutes:None schema:alternateName Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle
    206 schema:name Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle
    207 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...