Ontology type: schema:ScholarlyArticle
2002-05
AUTHORSHuilin Zhou, Jeffrey A. Ranish, Julian D. Watts, Ruedi Aebersold
ABSTRACTThe adaptation of sequences of chemical reactions to a solid-phase format has been essential to the automation, reproducibility, and efficiency of a number of biotechnological processes including peptide and oligonucleotide synthesis and sequencing1,2,3,4. Here we describe a method for the site-specific, stable isotopic labeling of cysteinyl peptides in complex peptide mixtures through a solid-phase capture and release process, and the concomitant isolation of the labeled peptides. The recovered peptides were analyzed by microcapillary liquid chromatography and tandem mass spectrometry (μLC-MS/MS) to determine their sequences and relative quantities. The method was used to detect galactose-induced changes in protein abundance in the yeast Saccharomyces cerevisiae. A side-by-side comparison with the isotope-coded affinity tag (ICAT) method5 demonstrated that the solid-phase method for stable isotope tagging of peptides is comparatively simpler, more efficient, and more sensitive. More... »
PAGES512-515
http://scigraph.springernature.com/pub.10.1038/nbt0502-512
DOIhttp://dx.doi.org/10.1038/nbt0502-512
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1023426571
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/11981568
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Chemical Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Analytical Chemistry",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0305",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Organic Chemistry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chromatography, Liquid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cysteine",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gas Chromatography-Mass Spectrometry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Isotopes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mass Spectrometry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Models, Chemical",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Peptides",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Saccharomyces cerevisiae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Time Factors",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle"
],
"type": "Organization"
},
"familyName": "Zhou",
"givenName": "Huilin",
"id": "sg:person.0720463627.62",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720463627.62"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle"
],
"type": "Organization"
},
"familyName": "Ranish",
"givenName": "Jeffrey A.",
"id": "sg:person.010125731727.05",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010125731727.05"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle"
],
"type": "Organization"
},
"familyName": "Watts",
"givenName": "Julian D.",
"id": "sg:person.0667127272.18",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667127272.18"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Institute for Systems Biology, 1441 North 34th Street, WA 98103-8904, Seattle"
],
"type": "Organization"
},
"familyName": "Aebersold",
"givenName": "Ruedi",
"id": "sg:person.015313356237.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015313356237.76"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/86777",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003596509",
"https://doi.org/10.1038/86777"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/85686",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050637706",
"https://doi.org/10.1038/85686"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/13690",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029022565",
"https://doi.org/10.1038/13690"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/310105a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006345158",
"https://doi.org/10.1038/310105a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1001-946",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029399357",
"https://doi.org/10.1038/nbt1001-946"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1016/1044-0305(94)80016-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018629105",
"https://doi.org/10.1016/1044-0305(94)80016-2"
],
"type": "CreativeWork"
}
],
"datePublished": "2002-05",
"datePublishedReg": "2002-05-01",
"description": "The adaptation of sequences of chemical reactions to a solid-phase format has been essential to the automation, reproducibility, and efficiency of a number of biotechnological processes including peptide and oligonucleotide synthesis and sequencing1,2,3,4. Here we describe a method for the site-specific, stable isotopic labeling of cysteinyl peptides in complex peptide mixtures through a solid-phase capture and release process, and the concomitant isolation of the labeled peptides. The recovered peptides were analyzed by microcapillary liquid chromatography and tandem mass spectrometry (\u03bcLC-MS/MS) to determine their sequences and relative quantities. The method was used to detect galactose-induced changes in protein abundance in the yeast Saccharomyces cerevisiae. A side-by-side comparison with the isotope-coded affinity tag (ICAT) method5 demonstrated that the solid-phase method for stable isotope tagging of peptides is comparatively simpler, more efficient, and more sensitive.",
"genre": "article",
"id": "sg:pub.10.1038/nbt0502-512",
"isAccessibleForFree": false,
"isFundedItemOf": [
{
"id": "sg:grant.2631587",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2381160",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2439911",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1115214",
"issn": [
"1087-0156",
"1546-1696"
],
"name": "Nature Biotechnology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "5",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "20"
}
],
"keywords": [
"complex peptide mixtures",
"isotope tagging",
"stable isotope tagging",
"solid-phase format",
"solid-phase method",
"solid-phase capture",
"microcapillary liquid chromatography",
"cysteinyl peptides",
"peptide mixtures",
"stable isotopic labeling",
"tandem mass",
"oligonucleotide synthesis",
"chemical reactions",
"mass spectrometry",
"isotopic labeling",
"liquid chromatography",
"concomitant isolation",
"peptides",
"biotechnological processes",
"quantitative proteome analysis",
"spectrometry",
"synthesis",
"chromatography",
"reaction",
"proteome analysis",
"relative quantities",
"mixture",
"reproducibility",
"method",
"process",
"capture",
"efficiency",
"side comparison",
"labeling",
"isolation",
"tagging",
"quantity",
"analysis",
"sequence",
"protein abundance",
"comparison",
"mass",
"abundance",
"changes",
"side",
"format",
"number",
"automation",
"yeast Saccharomyces",
"Saccharomyces",
"adaptation"
],
"name": "Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry",
"pagination": "512-515",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1023426571"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/nbt0502-512"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"11981568"
]
}
],
"sameAs": [
"https://doi.org/10.1038/nbt0502-512",
"https://app.dimensions.ai/details/publication/pub.1023426571"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:53",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_351.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/nbt0502-512"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0502-512'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0502-512'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0502-512'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0502-512'
This table displays all metadata directly associated to this object as RDF triples.
207 TRIPLES
21 PREDICATES
94 URIs
79 LITERALS
17 BLANK NODES