Ontology type: schema:ScholarlyArticle
2002-05
AUTHORSHuilin Zhou, Jeffrey A. Ranish, Julian D. Watts, Ruedi Aebersold
ABSTRACTThe adaptation of sequences of chemical reactions to a solid-phase format has been essential to the automation, reproducibility, and efficiency of a number of biotechnological processes including peptide and oligonucleotide synthesis and sequencing1,2,3,4. Here we describe a method for the site-specific, stable isotopic labeling of cysteinyl peptides in complex peptide mixtures through a solid-phase capture and release process, and the concomitant isolation of the labeled peptides. The recovered peptides were analyzed by microcapillary liquid chromatography and tandem mass spectrometry (μLC-MS/MS) to determine their sequences and relative quantities. The method was used to detect galactose-induced changes in protein abundance in the yeast Saccharomyces cerevisiae. A side-by-side comparison with the isotope-coded affinity tag (ICAT) method5 demonstrated that the solid-phase method for stable isotope tagging of peptides is comparatively simpler, more efficient, and more sensitive. More... »
PAGES512-515
http://scigraph.springernature.com/pub.10.1038/nbt0502-512
DOIhttp://dx.doi.org/10.1038/nbt0502-512
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/11981568
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