Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-04

AUTHORS

Amy S. Piatek, Sanjay Tyagi, Arno C. Pol, Amalio Telenti, Lincoln P. Miller, Fred Russell Kramer, David Alland

ABSTRACT

We developed a new approach to DNA sequence analysis that uses fluorogenic reporter molecules—molecular beacons—and demonstrated their ability to discriminate alleles in real-time PCR assays of genomic DNA. A set of overlapping molecular beacons was used to analyze an 81-bp region of the Mycobacterium tuberculosis rpoB gene for mutations that confer resistance to the antibiotic rifampin. In a blinded study of 52 rifampin-resistant and 23 rifampin-susceptible clinical isolates, this method correctly detected mutations in all of the resistant strains and in none of the susceptible strains. The assay was carried out entirely in sealed PCR tubes and was simple to perform and interpret. This approach can be used to analyze any DNA sequence of moderate length with single base pair accuracy. More... »

PAGES

359-363

Journal

TITLE

Nature Biotechnology

ISSUE

4

VOLUME

16

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0498-359

    DOI

    http://dx.doi.org/10.1038/nbt0498-359

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9555727


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