Molecular evolution by staggered extension process (StEP) in vitro recombination View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-03

AUTHORS

Huimin Zhao, Lori Giver, Zhixin Shao, Joseph A. Affholter, Frances H. Arnold

ABSTRACT

We have developed a simple and efficient method for in vitro mutagenesis and recombination of polynucleotide sequences. The staggered extension process (StEP) consists of priming the template sequence(s) followed by repeated cycles of denaturation and extremely abbreviated annealing/polymerase-catalyzed extension. In each cycle the growing fragments anneal to different templates based on sequence complementarity and extend further. This is repeated until full-length sequences form. Due to template switching, most of the polynucleotides contain sequence information from different parental sequences. The method is demonstrated by the recombination of two genes encoding thermostable subtilisins carrying two phenotypic markers separated by 113 base pairs and eight other point mutation markers. To demonstrate its utility for directed evolution, we have used StEP to recombine a set of five thermostabilized subtilisin E variants identified during a single round of error-prone PCR mutagenesis and screening. Screening the StEP-recombined library yielded an enzyme whose half-life at 65 degrees C is 50 times that of wild-type subtilisin E. More... »

PAGES

258

References to SciGraph publications

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  • Journal

    TITLE

    Nature Biotechnology

    ISSUE

    3

    VOLUME

    16

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0398-258

    DOI

    http://dx.doi.org/10.1038/nbt0398-258

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1017776064

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9528005


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