Engineering pathways for malate degradation in Saccharomyces cerevisiae View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1997-03

AUTHORS

H Volschenk, M Viljoen, J Grobler, B Petzold, F Bauer, R E Subden, R A Young, A Lonvaud, M Denayrolles, H J van Vuuren

ABSTRACT

Deacidification of grape musts is crucial for the production of well-balanced wines, especially in colder regions of the world. The major acids in wine are tartaric and malic acid. Saccharomyces cerevisiae cannot degrade malic acid efficiently due to the lack of a malate transporter and the low substrate affinity of its malic enzyme. We have introduced efficient pathways for malate degradation in S. cerevisiae by cloning and expressing the Schizosaccharomyces pombe malate permease (mae1) gene with either the S. pombe malic enzyme (mae2) or Lactococcus lactis malolactic (mleS) gene in this yeast. Under aerobic conditions, the recombinant strain expressing the mae1 and mae2 genes efficiently degraded 8 g/L of malate in a glycerol-ethanol medium within 7 days. The recombinant malolactic strain of S. cerevisiae (mae1 and mleS genes) fermented 4.5 g/L of malate in a synthetic grape must within 4 days. More... »

PAGES

253

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0397-253

DOI

http://dx.doi.org/10.1038/nbt0397-253

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1056449394

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9062925


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrolysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lactococcus lactis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malate Dehydrogenase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Transport Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organic Anion Transporters", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Schizosaccharomyces", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Stellenbosch University", 
          "id": "https://www.grid.ac/institutes/grid.11956.3a", 
          "name": [
            "Department of Microbiology, University of Stellenbosch, South Africa."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Volschenk", 
        "givenName": "H", 
        "id": "sg:person.0646123220.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646123220.41"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Viljoen", 
        "givenName": "M", 
        "id": "sg:person.01137624462.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137624462.00"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Grobler", 
        "givenName": "J", 
        "id": "sg:person.0621200622.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0621200622.40"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Petzold", 
        "givenName": "B", 
        "type": "Person"
      }, 
      {
        "familyName": "Bauer", 
        "givenName": "F", 
        "id": "sg:person.01034623507.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034623507.30"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Subden", 
        "givenName": "R E", 
        "type": "Person"
      }, 
      {
        "familyName": "Young", 
        "givenName": "R A", 
        "id": "sg:person.011705017367.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011705017367.87"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Lonvaud", 
        "givenName": "A", 
        "id": "sg:person.013305357773.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013305357773.18"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Denayrolles", 
        "givenName": "M", 
        "id": "sg:person.010113205374.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010113205374.82"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "van Vuuren", 
        "givenName": "H J", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1574-6968.1995.tb07332.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006666566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.1995.tb07332.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006666566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(85)80305-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010172810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(92)90159-m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014136225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(92)90159-m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014136225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(85)90120-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017120147"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(85)90120-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017120147"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0740-0020(95)80080-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017176377"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/yea.320100506", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027694284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/yea.320100506", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027694284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(19960315)12:3<215::aid-yea903>3.0.co;2-m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030086723"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(19960315)12:3<215::aid-yea903>3.0.co;2-m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030086723"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90185-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031424271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90185-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031424271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2170585", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031563704"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2170585", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031563704"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0926-6593(65)80080-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034347549"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0926-6593(65)80080-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034347549"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00310817", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036918654", 
          "https://doi.org/10.1007/bf00310817"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00310817", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036918654", 
          "https://doi.org/10.1007/bf00310817"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-2628(08)60234-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037542395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00422202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043272645", 
          "https://doi.org/10.1007/bf00422202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1990.tb19456.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043479730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(93)80488-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049004832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90005-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049548272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90005-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049548272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00422193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051005588", 
          "https://doi.org/10.1007/bf00422193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077156426", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079183246", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080251885", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a129850", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1080323411"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1997-03", 
    "datePublishedReg": "1997-03-01", 
    "description": "Deacidification of grape musts is crucial for the production of well-balanced wines, especially in colder regions of the world. The major acids in wine are tartaric and malic acid. Saccharomyces cerevisiae cannot degrade malic acid efficiently due to the lack of a malate transporter and the low substrate affinity of its malic enzyme. We have introduced efficient pathways for malate degradation in S. cerevisiae by cloning and expressing the Schizosaccharomyces pombe malate permease (mae1) gene with either the S. pombe malic enzyme (mae2) or Lactococcus lactis malolactic (mleS) gene in this yeast. Under aerobic conditions, the recombinant strain expressing the mae1 and mae2 genes efficiently degraded 8 g/L of malate in a glycerol-ethanol medium within 7 days. The recombinant malolactic strain of S. cerevisiae (mae1 and mleS genes) fermented 4.5 g/L of malate in a synthetic grape must within 4 days.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nbt0397-253", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1115214", 
        "issn": [
          "1087-0156", 
          "1546-1696"
        ], 
        "name": "Nature Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "Engineering pathways for malate degradation in Saccharomyces cerevisiae", 
    "pagination": "253", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "def2b5339b0b6ba6fc02a90af7779963c8c408b51603412a157d21957a200246"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9062925"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9604648"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nbt0397-253"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1056449394"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nbt0397-253", 
      "https://app.dimensions.ai/details/publication/pub.1056449394"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000442.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nbt0397-253"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0397-253'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0397-253'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0397-253'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0397-253'


 

This table displays all metadata directly associated to this object as RDF triples.

227 TRIPLES      21 PREDICATES      61 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nbt0397-253 schema:about N00d9c9be93e744348028560866feb06e
2 N3a095f42eff347f6badec210a0400add
3 N3d0ddecdd9ae4ea990163012af077207
4 N4a4d42670b844db884ead2d9c076016b
5 N599c38bf37324020b6abe3ec9c4e9c00
6 Na56bd5392818450796350d999c7f3d11
7 Nabf100ddef73404bbbc9727b8293f7a7
8 Nbdfc0f1cb647416e8414b31a94e09058
9 Nc3615beba917452dab601b2a55b98fd1
10 Ncf68fd4a639249ba91d2479353d8db5d
11 Ne408450f19524f3daaf008394ba5719e
12 anzsrc-for:06
13 anzsrc-for:0601
14 schema:author Nee63e8404617482d802e155214aef6f4
15 schema:citation sg:pub.10.1007/bf00310817
16 sg:pub.10.1007/bf00422193
17 sg:pub.10.1007/bf00422202
18 https://app.dimensions.ai/details/publication/pub.1077156426
19 https://app.dimensions.ai/details/publication/pub.1079183246
20 https://app.dimensions.ai/details/publication/pub.1080251885
21 https://doi.org/10.1002/(sici)1097-0061(19960315)12:3<215::aid-yea903>3.0.co;2-m
22 https://doi.org/10.1002/yea.320100506
23 https://doi.org/10.1016/0014-5793(85)80305-7
24 https://doi.org/10.1016/0014-5793(93)80488-g
25 https://doi.org/10.1016/0378-1119(85)90120-9
26 https://doi.org/10.1016/0378-1119(88)90005-4
27 https://doi.org/10.1016/0378-1119(88)90185-0
28 https://doi.org/10.1016/0378-1119(92)90159-m
29 https://doi.org/10.1016/s0065-2628(08)60234-7
30 https://doi.org/10.1016/s0740-0020(95)80080-8
31 https://doi.org/10.1016/s0926-6593(65)80080-7
32 https://doi.org/10.1042/bj2170585
33 https://doi.org/10.1093/oxfordjournals.jbchem.a129850
34 https://doi.org/10.1111/j.1432-1033.1990.tb19456.x
35 https://doi.org/10.1111/j.1574-6968.1995.tb07332.x
36 schema:datePublished 1997-03
37 schema:datePublishedReg 1997-03-01
38 schema:description Deacidification of grape musts is crucial for the production of well-balanced wines, especially in colder regions of the world. The major acids in wine are tartaric and malic acid. Saccharomyces cerevisiae cannot degrade malic acid efficiently due to the lack of a malate transporter and the low substrate affinity of its malic enzyme. We have introduced efficient pathways for malate degradation in S. cerevisiae by cloning and expressing the Schizosaccharomyces pombe malate permease (mae1) gene with either the S. pombe malic enzyme (mae2) or Lactococcus lactis malolactic (mleS) gene in this yeast. Under aerobic conditions, the recombinant strain expressing the mae1 and mae2 genes efficiently degraded 8 g/L of malate in a glycerol-ethanol medium within 7 days. The recombinant malolactic strain of S. cerevisiae (mae1 and mleS genes) fermented 4.5 g/L of malate in a synthetic grape must within 4 days.
39 schema:genre research_article
40 schema:inLanguage en
41 schema:isAccessibleForFree true
42 schema:isPartOf N03aea318009a4d018c0ed9d61eed4705
43 N9cd8ced4ec6c40108840a234693e3d0e
44 sg:journal.1115214
45 schema:name Engineering pathways for malate degradation in Saccharomyces cerevisiae
46 schema:pagination 253
47 schema:productId N047259fc68fd4db680edbfe7badf3b7f
48 N0d4ccdfe070f4befac5d0e0465daf0c7
49 N586d9a24f6374b7d83e7ded40e6b9951
50 N90af4b5fd3d04a67b469acd0927d1c75
51 Ne0790eff7ccc438b8a5da835ac8f9181
52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056449394
53 https://doi.org/10.1038/nbt0397-253
54 schema:sdDatePublished 2019-04-10T13:01
55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
56 schema:sdPublisher Nc3bdbd68dda04405b3fd3618a8505155
57 schema:url https://www.nature.com/articles/nbt0397-253
58 sgo:license sg:explorer/license/
59 sgo:sdDataset articles
60 rdf:type schema:ScholarlyArticle
61 N00d9c9be93e744348028560866feb06e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Membrane Transport Proteins
63 rdf:type schema:DefinedTerm
64 N03aea318009a4d018c0ed9d61eed4705 schema:volumeNumber 15
65 rdf:type schema:PublicationVolume
66 N047259fc68fd4db680edbfe7badf3b7f schema:name doi
67 schema:value 10.1038/nbt0397-253
68 rdf:type schema:PropertyValue
69 N0cbb1c7469f142129c6e40e30318a968 rdf:first N2b11753010a546ddaf8bf961d825470b
70 rdf:rest Neaf5606e515a44f08f02ba833adf1873
71 N0d4ccdfe070f4befac5d0e0465daf0c7 schema:name pubmed_id
72 schema:value 9062925
73 rdf:type schema:PropertyValue
74 N1b7eff4428af4ed79e2b1225c66d9c5b rdf:first sg:person.013305357773.18
75 rdf:rest Nf45f8084d89744c09d064eb2d306d784
76 N2b11753010a546ddaf8bf961d825470b schema:familyName Subden
77 schema:givenName R E
78 rdf:type schema:Person
79 N30c7a01be1a94bccbc83829296e206c0 rdf:first N5779bd16d30e468dab6ff7c6d411a854
80 rdf:rest rdf:nil
81 N3a095f42eff347f6badec210a0400add schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Bacterial Proteins
83 rdf:type schema:DefinedTerm
84 N3d0ddecdd9ae4ea990163012af077207 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Hydrolysis
86 rdf:type schema:DefinedTerm
87 N4a4d42670b844db884ead2d9c076016b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Saccharomyces cerevisiae
89 rdf:type schema:DefinedTerm
90 N5779bd16d30e468dab6ff7c6d411a854 schema:familyName van Vuuren
91 schema:givenName H J
92 rdf:type schema:Person
93 N586d9a24f6374b7d83e7ded40e6b9951 schema:name nlm_unique_id
94 schema:value 9604648
95 rdf:type schema:PropertyValue
96 N599c38bf37324020b6abe3ec9c4e9c00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Malates
98 rdf:type schema:DefinedTerm
99 N90af4b5fd3d04a67b469acd0927d1c75 schema:name dimensions_id
100 schema:value pub.1056449394
101 rdf:type schema:PropertyValue
102 N9afdee5779044d7ea35cc18f85732ba3 rdf:first sg:person.01034623507.30
103 rdf:rest N0cbb1c7469f142129c6e40e30318a968
104 N9cd8ced4ec6c40108840a234693e3d0e schema:issueNumber 3
105 rdf:type schema:PublicationIssue
106 Na2433b61cdac4b68855a45171b42f97a rdf:first sg:person.01137624462.00
107 rdf:rest Nd443b331772f42db9608bd7aa6f1ae77
108 Na56bd5392818450796350d999c7f3d11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Genes, Fungal
110 rdf:type schema:DefinedTerm
111 Nabf100ddef73404bbbc9727b8293f7a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Schizosaccharomyces
113 rdf:type schema:DefinedTerm
114 Nbb0a6882aea14912b2674c1e1e7919a0 schema:familyName Petzold
115 schema:givenName B
116 rdf:type schema:Person
117 Nbd71dc16cf7143d6a7c5e05a19e0fb2a rdf:first Nbb0a6882aea14912b2674c1e1e7919a0
118 rdf:rest N9afdee5779044d7ea35cc18f85732ba3
119 Nbdfc0f1cb647416e8414b31a94e09058 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Recombinant Proteins
121 rdf:type schema:DefinedTerm
122 Nc3615beba917452dab601b2a55b98fd1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Lactococcus lactis
124 rdf:type schema:DefinedTerm
125 Nc3bdbd68dda04405b3fd3618a8505155 schema:name Springer Nature - SN SciGraph project
126 rdf:type schema:Organization
127 Ncf68fd4a639249ba91d2479353d8db5d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Malate Dehydrogenase
129 rdf:type schema:DefinedTerm
130 Nd443b331772f42db9608bd7aa6f1ae77 rdf:first sg:person.0621200622.40
131 rdf:rest Nbd71dc16cf7143d6a7c5e05a19e0fb2a
132 Ne0790eff7ccc438b8a5da835ac8f9181 schema:name readcube_id
133 schema:value def2b5339b0b6ba6fc02a90af7779963c8c408b51603412a157d21957a200246
134 rdf:type schema:PropertyValue
135 Ne408450f19524f3daaf008394ba5719e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Organic Anion Transporters
137 rdf:type schema:DefinedTerm
138 Neaf5606e515a44f08f02ba833adf1873 rdf:first sg:person.011705017367.87
139 rdf:rest N1b7eff4428af4ed79e2b1225c66d9c5b
140 Nee63e8404617482d802e155214aef6f4 rdf:first sg:person.0646123220.41
141 rdf:rest Na2433b61cdac4b68855a45171b42f97a
142 Nf45f8084d89744c09d064eb2d306d784 rdf:first sg:person.010113205374.82
143 rdf:rest N30c7a01be1a94bccbc83829296e206c0
144 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
145 schema:name Biological Sciences
146 rdf:type schema:DefinedTerm
147 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
148 schema:name Biochemistry and Cell Biology
149 rdf:type schema:DefinedTerm
150 sg:journal.1115214 schema:issn 1087-0156
151 1546-1696
152 schema:name Nature Biotechnology
153 rdf:type schema:Periodical
154 sg:person.010113205374.82 schema:familyName Denayrolles
155 schema:givenName M
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010113205374.82
157 rdf:type schema:Person
158 sg:person.01034623507.30 schema:familyName Bauer
159 schema:givenName F
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034623507.30
161 rdf:type schema:Person
162 sg:person.01137624462.00 schema:familyName Viljoen
163 schema:givenName M
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137624462.00
165 rdf:type schema:Person
166 sg:person.011705017367.87 schema:familyName Young
167 schema:givenName R A
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011705017367.87
169 rdf:type schema:Person
170 sg:person.013305357773.18 schema:familyName Lonvaud
171 schema:givenName A
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013305357773.18
173 rdf:type schema:Person
174 sg:person.0621200622.40 schema:familyName Grobler
175 schema:givenName J
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0621200622.40
177 rdf:type schema:Person
178 sg:person.0646123220.41 schema:affiliation https://www.grid.ac/institutes/grid.11956.3a
179 schema:familyName Volschenk
180 schema:givenName H
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646123220.41
182 rdf:type schema:Person
183 sg:pub.10.1007/bf00310817 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036918654
184 https://doi.org/10.1007/bf00310817
185 rdf:type schema:CreativeWork
186 sg:pub.10.1007/bf00422193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051005588
187 https://doi.org/10.1007/bf00422193
188 rdf:type schema:CreativeWork
189 sg:pub.10.1007/bf00422202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043272645
190 https://doi.org/10.1007/bf00422202
191 rdf:type schema:CreativeWork
192 https://app.dimensions.ai/details/publication/pub.1077156426 schema:CreativeWork
193 https://app.dimensions.ai/details/publication/pub.1079183246 schema:CreativeWork
194 https://app.dimensions.ai/details/publication/pub.1080251885 schema:CreativeWork
195 https://doi.org/10.1002/(sici)1097-0061(19960315)12:3<215::aid-yea903>3.0.co;2-m schema:sameAs https://app.dimensions.ai/details/publication/pub.1030086723
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1002/yea.320100506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027694284
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/0014-5793(85)80305-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010172810
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/0014-5793(93)80488-g schema:sameAs https://app.dimensions.ai/details/publication/pub.1049004832
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/0378-1119(85)90120-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017120147
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/0378-1119(88)90005-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049548272
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/0378-1119(88)90185-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031424271
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/0378-1119(92)90159-m schema:sameAs https://app.dimensions.ai/details/publication/pub.1014136225
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s0065-2628(08)60234-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037542395
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/s0740-0020(95)80080-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017176377
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/s0926-6593(65)80080-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034347549
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1042/bj2170585 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031563704
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1093/oxfordjournals.jbchem.a129850 schema:sameAs https://app.dimensions.ai/details/publication/pub.1080323411
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1111/j.1432-1033.1990.tb19456.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043479730
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1111/j.1574-6968.1995.tb07332.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006666566
224 rdf:type schema:CreativeWork
225 https://www.grid.ac/institutes/grid.11956.3a schema:alternateName Stellenbosch University
226 schema:name Department of Microbiology, University of Stellenbosch, South Africa.
227 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...