Molecular Beacons: Probes that Fluoresce upon Hybridization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-03

AUTHORS

S Tyagi, F R Kramer

ABSTRACT

We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization does not occur when the target contains a mismatched nucleotide or a deletion. The probes are particularly suited for monitoring the synthesis of specific nucleic acids in real time. When used in nucleic acid amplification assays, gene detection is homogeneous and sensitive, and can be carried out in a sealed tube. When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced. More... »

PAGES

303

References to SciGraph publications

Journal

TITLE

Nature Biotechnology

ISSUE

3

VOLUME

14

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  • Methods Of Using Fet Labeled Oligonucleotides That Include A 3′-5′ Exonuclease Resistant Quencher Domain And Compositions For Practicing The Same
  • Digital Amplification
  • Prostate Cancer Prognostic Compositions And Kits
  • Primer Middle Sequence Interference Pcr Technology
  • Compositions Of Toehold Primer Duplexes And Methods Of Use
  • Primers And Methods For Nucleic Acid Amplification
  • Oligonucleotide Conjugates
  • Method And Apparatus For Generating Thermal Melting Curves In A Microfluidic Device
  • Uv Excitable Fluorescent Energy Transfer Dyes
  • Microfluidic Particle-Analysis Systems
  • Methods And Compositions For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Pca3, Pca3 Genes, And Methods Of Use
  • Method To Detect Prostate Cancer In A Sample
  • Hybridization Chain Reaction Amplification For In Situ Imaging
  • Methods And Compositions For Multiplexed And Ultrasensitive Microrna Detection
  • Detection Of Small Nucleic Acids
  • Method For Simultaneously Performing Multiple Amplification Reactions
  • Method For Performing An Assay With A Nucleic Acid Present In A Specimen
  • Ultra High-Throughput Opti-Nanopore Dna Readout Platform
  • Diagnosis And Treatment Of Alzheimer's Disease
  • Isolation Of Living Cells And Preparation Of Cell Lines Based On Detection And Quantification Of Preselected Cellular Ribonucleic Acid Sequences
  • Signal Amplification With Lollipop Probes
  • Method For Activating A Nucleic Acid For A Polymerase Reaction
  • Method Of Making A Microbead Array With Attached Biomolecules
  • Nucleic Acid Amplification Using Microfluidic Devices
  • Creation Of Functionalized Microparticle Libraries By Oligonucleotide Ligation Or Elongation
  • Microfluidic Device And Methods Of Using Same
  • Delivery Device
  • Markers Of Definitive Endoderm
  • Dark Quenchers For Donor-Acceptor Energy Transfer
  • Polyvalent Rna-Nanoparticle Compositions
  • Methods For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Microfabricated Structure Having Parallel And Orthogonal Flow Channels Controlled By Row And Column Multiplexors
  • Method For Using Probe Based Pcr Detection To Measure The Levels Of Circulating Demethylated Beta Cell Derived Dna As A Measure Of Beta Cell Loss In Diabetes
  • Detectably Labeled Dual Conformation Oligonucleotide Probes, Assays And Kits
  • Methods Of Analyzing Polynucleotides Employing Energy Transfer Dyes
  • Multiple Reporter Read-Out For Bioassays
  • Methods Of Analyzing Polynucleotides Employing Energy Transfer Dyes
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  • Oligonucleotide Probes Bearing Quenchable Fluorescent Labels, And Methods Of Use Thereof
  • Hybridization Assay Using Self-Quenching Fluorescence Probe
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  • Method For Detecting Point Mutations In Dna Utilizing Fluorescence Energy Transfer
  • Hybridization Assay Using Self-Quenching Fluorescence Probe
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  • Methods, Kits And Compositions Pertaining To Pna Molecular Beacons
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  • Methods And Probes For Detecting A Vancomycin Resistance Gene
  • Triggered Rnai
  • Through Bond Energy Transfer In Fluorescent Dyes For Labelling Biological Molecules
  • Methods And Kits For Nucleic Acid Analysis Using Fluorescence Resonance Energy Transfer
  • Zymogenic Nucleic Acid Molecules
  • Fluorescence-Mediated Molecular Tomography
  • System And Method For Carrying Out And Monitoring Biological Processes
  • Membrane-Permeant Peptide Complexes For Medical Imaging, Diagnostics, And Pharmaceutical Therapy
  • Membrane-Permeant Peptide Complexes For Medical Imaging, Diagnostics, And Pharmaceutical Therapy
  • Rapid And Efficient Capture Of Dna From Sample Without Using Cell Lysing Reagent
  • Templated Nanoconjugates
  • Microrna Compounds And Methods For Modulating Mir-122
  • Detection Probes, Kits And Assays
  • Method For Detecting Pathogens Using Molecular Beacons
  • Detection Method Using Dissociated Rolling Circle Amplification
  • Process For High Throughput Dna Methylation Analysis
  • Charge Switch Nucleotides
  • Molecular Modification Assays
  • Improved Methods For Cdna Synthesis
  • Nonlinear Optical Detection Of Molecules Comprising An Unnatural Amino Acid Possessing A Hyperpolarizability
  • Amphiphilic Substances And Functionalized Lipid Vesicles Including The Same
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  • System And Method Including Multiple Processing Lanes Executing Processing Protocols
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  • Chromosome Conformation Capture In Partitions
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  • Methods And Systems For Image Processing Of Microfluidic Devices
  • Apparatus And Methods For Conducting Assays And High Throughput Screening
  • Particles For Detecting Intracellular Targets
  • Assays For Short Sequence Variants
  • Compositions And Methods For Enhanced Sensitivity And Specificity Of Nucleic Acid Synthesis
  • Optimization Of Gene Expression Analysis Using Immobilized Capture Probes
  • Instruments And Methods For Mixing The Contents Of A Detection Chamber
  • Reliable Fluorescence Correction Method For Two-Color Measurement Fluorescence System
  • Methods, Kits And Reaction Mixtures For Analyzing Single-Stranded Nucleic Acid Sequences
  • Identifiers

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