Molecular Beacons: Probes that Fluoresce upon Hybridization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-03

AUTHORS

S Tyagi, F R Kramer

ABSTRACT

We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization does not occur when the target contains a mismatched nucleotide or a deletion. The probes are particularly suited for monitoring the synthesis of specific nucleic acids in real time. When used in nucleic acid amplification assays, gene detection is homogeneous and sensitive, and can be carried out in a sealed tube. When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced. More... »

PAGES

303

References to SciGraph publications

Journal

TITLE

Nature Biotechnology

ISSUE

3

VOLUME

14

Related Patents

  • Nucleic Acid Enzyme Biosensors For Ions
  • Nanostructures That Provide A Modified Nanoenvironment For The Enhancement Of Luminescence
  • Sequences For Detection And Identification Of Methicillin-Resistant Staphylococcus Aureus (Mrsa)
  • Nucleic Acid Amplification Method
  • Digital Amplification
  • Oligonucleotides And Analogs Labeled With Energy Transfer Dyes
  • Sulfonated Diarylrhodamine Dyes
  • Polynucleotide And Polypeptide Sequences Associated With Herbicide Tolerance
  • Labeled Oligonucleotide
  • Reverse Transcription And Amplification Of Rna With Simultaneous Degradation Of Dna
  • Nucleic Acid Enrichment
  • Mrna Ratios In Urinary Sediments And/Or Urine As A Prognostic And/Or Theranostic Marker For Prostate Cancer
  • Compositions And Methods For Detecting Klebsiella Pneumoniae
  • Method For Identifying Nucleotide Polymorphism
  • Nucleic Acid Functionalized Nanoparticles For Therapeutic Applications
  • Multi-Color Time Resolved Fluorophores Based On Macrocyclic Lanthanide Complexes
  • Kits For Multiplexed Nucleic Acid Analysis By Capture Of Single-Stranded Dna Produced From Double-Stranded Target Fragments
  • Split Dna Enzyme For Visual Single Nucleotide Polymorphism Typing
  • Method For Detecting The Presence Of A Nucleic Acid In A Sample
  • Stabilized Nucleic Acid Dark Quencher-Fluorophore Probes
  • Method For Detecting Oligonucleotides Using Energy Transfer Dyes With Long Stoke Shift
  • Methods, Kits And Compositions Pertaining To Pna Molecular Beacons
  • Probe For Detecting Point Mutations In Dna Utilizing Fluorescence Energy Transfer
  • Protein Expression Profiling
  • Nucleic Acid Amplification Utilizing Microfluidic Devices
  • Purification Of Cell Mixtures Using Molecular Beacons Targeting Cell Specific Rna
  • Method For Analyzing Target Nucleic Acid, Kit, And Analyzer
  • Digital Amplification
  • Assay Involving Looped Nucleic Acid
  • In-Situ Methods For Analyzing Target Sequences Using Linear Beacons
  • Reverse Displacement Assay For Detection Of Nucleic Acid Sequences
  • Devices And Methods For Detecting Nucleic Acid Analytes In Samples
  • Stabilized Nucleic Acid Dark Quencher-Fluorophore Probes
  • Methods For Manipulating Liquid Substances In Multi-Chambered Receptacles
  • System And Method For Monitoring For Dna Amplification By Fluorescence
  • Detection Of Nucleic Acid Using Linear Beacons
  • Analysis, Secure Access To, And Transmission Of Array Images
  • 2'-Terminator Related Pyrophosphorolysis Activated Polymerization
  • Method Using A Nonlinear Optical Technique For Detection Of Interactions Involving A Conformational Change
  • Hiv Type And Subtype Detection
  • Dual Resonance Energy Transfer Nucleic Acid Probes
  • Methods For Detection Of A Target Nucleic Acid By Capture Using Multi-Subunit Probes
  • Methods And Compositions For Enrichment Of Nucleic Acids In Mixtures Of Highly Homologous Sequences
  • Preprimitive Streak And Mesendoderm Cells
  • Luminescent 1-Hydroxy-2-Pyridinone Chelates Of Lanthanides
  • Reagent Cartridge
  • Methods, Compositions, And Kits For Detecting Allelic Variants
  • Instruments For Mixing The Contents Of A Detection Chamber
  • Patch Applying Apparatus
  • Development Of A Highly Sensitive Quantification System For Assessing Dna Degradation And Quality In Forensic Samples
  • Nucleic Acid Processing Kits And Devices
  • Method And Kit For Performing A Colorectal Cancer Assay
  • Optical Lens System And Method For Microfluidic Devices
  • Primers And Methods For The Detection And Discrimination Of Nucleic Acids
  • Methods Of Concentrating An Analyte
  • Compositions And Methods For Detection And Isolation Of Phosphorylated Molecules
  • Caged Sensors, Regulators And Compounds And Uses Thereof
  • Modified Probe Molecules Having Self-Complementary Regions
  • Method For Detecting A Structured Target
  • Instruments And Methods For Mixing The Contents Of A Detection Chamber
  • Number Coding For Identification Of Subtypes Of Coded Types Of Solid Phase Carriers
  • Flowcell Systems For Single Molecule Detection
  • Evolving New Molecular Function
  • Biomolecular Self-Assembly
  • Combination Of Dsdna Binding Dye And Probes For Characterization Of Ssdna Sequences
  • Benzopyrylium Compounds
  • Method For Deblocking Of Labeled Oligonucleotides
  • Method Of Delivering Material Or Stimulus To A Biological Subject
  • Channels With Cross-Sectional Thermal Gradients
  • Microfluidic Device And Methods Of Using Same
  • Method For Determining The Presence Of An Rna Analyte In A Sample Using A Modified Oligonucleotide Probe
  • Pipettor Including Compliant Coupling
  • Molecular Torches And Their Use Under Denaturing Conditions
  • System And Method For Processing Samples
  • Dark Quenchers For Donor-Acceptor Energy Transfer
  • Automated Analysis Of Multiplexed Probe-Target Interaction Patterns: Pattern Matching And Allele Identification
  • Nucleic Acid Functionalized Nonoparticles For Therapeutic Applications
  • Methods, Apparatus, And Computer Programs For Verifying The Integrity Of A Probe
  • Amplification And Detection Method
  • Method For Detecting A Target Nucleic Acid Sequence
  • Hydrodabcyl
  • Intramolecularly-Quenched Near Infrared Fluorescent Probes
  • Affinity-Shifted Probes For Quantifying Analyte Polynucleotides
  • Specimen Container Detection
  • Gray Leaf Spot Tolerant Maize And Methods Of Production
  • Method And Program For Performing Baseline Correction Of Amplification Curves In A Pcr Experiment
  • Pkr Activation Via Hybridization Chain Reaction
  • Stabilized Nucleic Acid Dark Quencher-Fluorophore Probes
  • Methods For Detection And Identification Of Methicillin-Resistant Staphylococcus Aureus
  • Nucleic Acid-Based Detection
  • Single Probe, Multiple Temperature, Nucleic Acid Detection Methods, Kits, And Compositions
  • Purification Of Cell Mixtures Using Molecular Beacons Targeting Cell Specific Rna
  • Automated Analyzer For Performing A Nucleic Acid-Based Assay
  • Methods Of Labeling Nucleic Acids With Energy Transfer Dyes
  • Apparatus And Methods For Conducting Assays And High Throughput Screening
  • Engineered Lectin Oligomers With Antiviral Activity
  • Method For Detecting Human Papillomavirus Mrna
  • Method And Kit For The Prognosis Of Colorectal Cancer
  • A Molecular Beacon And A Method For Its Synthesis
  • Hybridization Chain Reaction Amplification For In Situ Imaging
  • Polynucleotides For The Detection Of Escherichia Coli O157:H7 And Escherichia Coli O157:Nm Verotoxin Producers
  • Specific Double-Stranded Probes For Homogeneous Detection Of Nucleic Acid And Their Application Methods
  • Lateral Flow Devices
  • Microfluidic Device And Methods Of Using Same
  • Hppd-Inhibitor Herbicide Tolerance
  • Fluorescent Chemical Compounds Having High Selectivity For Double Stranded Dna, And Methods For Their Use
  • Ultra High-Throughput Opti-Nanopore Dna Readout Platform
  • Stable Hybrid
  • Charge Switch Nucleotides
  • Evolving New Molecular Function
  • A Molecular Beacon And A Method For Its Synthesis
  • Hybridization Chain Reaction Amplification For In Situ Imaging
  • Fluorescence Based Biosensor
  • Membrane-Permeant Peptide Complexes For Treatment Of Sepsis
  • System And Method Including Analytical Units
  • Methods For Manipulating Liquid Substances In Multi-Chambered Receptacles
  • System And Method Including Analytical Units
  • Use Of An Extraction Control In A Method Of Extracting Nucleic Acids
  • Sensor Housing And Reagent Chemistry
  • Dark Quenchers For Donor-Acceptor Energy Transfer
  • Pca3 Messenger Rna Species In Benign And Malignant Prostate Tissues
  • Primers And Probes For Detection And Discrimination Of Types And Subtypes Of Influenza Viruses
  • Method To Detect Prostate Cancer In A Sample
  • Fluorescent Imaging Agents
  • Reaction Vessel
  • Compositions Of Toehold Primer Duplexes And Methods Of Use
  • Nucleic Acid Base Analogs With Quenching And Fluorescent Activities And Applications Thereof
  • Assays Employing Electrochemiluminescent Labels And Electrochemiluminescence Quenchers
  • Channels With Cross-Sectional Thermal Gradients
  • Gravity-Assisted Mixing Methods
  • Probe And Method For Dna Detection
  • Method For Identifying Polymorphism Of Nucleic Acid Molecules
  • Primers And Methods For Nucleic Acid Amplification
  • Methods For Increasing Definitive Endoderm Production
  • Method For Detecting The Presence Of A Nucleic Acid In A Sample
  • Sieving Of Nucleic Acid Samples
  • Hybridization Chain Reaction
  • Primers And Methods For The Detection And Discrimination Of Nucleic Acids
  • Single Cell Based Reporter Assay To Monitor Gene Expression Patterns With High Spatio-Temporal Resolution
  • Evolving New Molecular Function
  • Oligonucleotides And Analogs Labeled With Energy Transfer Dyes
  • Sequence-Specific Detection Of Nucleotide Sequences
  • Triggered Molecular Geometry Based Bioimaging Probes
  • Optical Lens System And Method For Microfluidic Devices
  • Methods And Compositions For Detection Of A Target Nucleic Acid Sequence Utilizing A Probe With A 3′ Flap
  • Microorganism Nucleic Acid Purification From Host Samples
  • Triggered Rnai
  • Prostate Cancer Prognostic Compositions And Kits
  • Luminescent Macrocyclic Lanthanide Complexes
  • Dna Mutation Mapping By Multiple Energy Transfer Interactions
  • Zymogenic Nucleic Acid Detection Methods, And Related Molecules And Kits
  • Duplex Probes For Hybridization Reactions
  • Modified Nucleic Acid Probes And Use Thereof
  • Competitive Immunoassay
  • Method Of Amplifying Whole Genomes Without Subjecting The Genome To Denaturing Conditions
  • Methods For Suppressing The Binding Of Detectable Probes To Non-Target Sequences In Hybridization Assays
  • Method For Nucleic Acid Analysis Using Fluorescence Resonance Energy Transfer
  • Devices And Methods For Detecting Target Molecules In Biological Samples
  • Single-Molecule Selection Methods And Compositions Therefrom
  • Beta 2 Adrenergic Polymorphism Detection
  • Nucleic Acid Sequencing Using Charge-Switch Nucleotides
  • Energy Transfer Dyes With Enhanced Fluorescence
  • Molecular Torches
  • Pca3 Genes
  • Homogeneous Detection Of A Target Through Nucleic Acid Ligand-Ligand Beacon Interaction
  • Non-Competitive Co-Amplification Methods
  • Absolute Pcr Quantification
  • Diagnosis And Treatment Of Drug-Resistant Ewing's Sarcoma
  • Novel Use Of Fluorescence Resonance Energy Transfer
  • Homogeneous Multiplex Screening Assays And Kits
  • Apparatus And Methods For Conducting Assays And High Throughput Screening
  • Robotic Arm
  • Specific Double-Stranded Probes For Homogeneous Detection Of Nucleic Acid And Their Application Methods
  • Hybridization Chain Reaction
  • Uv Excitable Energy Transfer Reagents
  • Method For Using Probe Based Pcr Detection To Measure The Levels Of Circulating Demethylated Beta Cell Derived Dna As A Measure Of Beta Cell Loss In Diabetes
  • Method For Determining An Attribute Profile Of Biological Samples
  • Detection Of Micro-Organisms Based On Their Nad-Dependent Dna Ligase Activity
  • Methods For Identifying Factors For Differentiating Definitive Endoderm
  • Compositions For Detecting Small Rnas
  • Digital Amplification
  • Specialized Oligonucleotides And Their Use In Nucleic Acid Amplification And Detection
  • Aromatic Triamide-Lanthanide Complexes
  • Nucleic Acid Probe, Method For Designing Nucleic Acid Probe, And Method For Detecting Target Sequence
  • System And Method For Controlling Thermal Cycler Modules
  • Methods For Identifying Aptamer Regulators
  • Affinity-Based Partition Assay For Detection Of Target Molecules
  • Microrna Compounds And Methods For Modulating Mir-122
  • Detection Of Staphylococcus Aureus And Identification Of Methicillin-Resistant Staphylococcus Aureus
  • Digital Amplification
  • Molecular Torches And Their Use Under Strand Displacement Conditions
  • Digital Amplification
  • Detection Of Target Nucleic Acid And Variants
  • Magnetic Damping For Specimen Transport System
  • Hybridization Chain Reaction Amplification For In Situ Imaging
  • Fluorescence-Mediated Molecular Tomography
  • Detection Of Penicillin Tolerance In Group B Streptococcus: Single Nucleotide Polymorphisms In Penicillin Binding Protein 4
  • Methods And Compositions To Detect Nucleic Acids In A Biological Sample
  • Nanoconjugates Able To Cross The Blood-Brain Barrier
  • Container For Carrying Out And Monitoring Biological Processes
  • Microfluidic Reaction Apparatus For High Throughput Screening
  • Multi-Aptamer-Based, Cell-Specific, One-Step Tumor Cell Detection Assays
  • Method And Apparatus For Generating Thermal Melting Curves In A Microfluidic Device
  • Templated Nanoconjugates
  • Digital Amplification
  • Magnetic Damping For Specimen Transport System
  • Microfluidic Particle-Analysis Systems
  • Method For Amplification-Free Nucleic Acid Detection On Optofluidic Chips
  • System And Method Including Analytical Units
  • Enzymatic Nucleic Acid Synthesis: Compositions And Methods For Inhibiting Pyrophosphorolysis
  • Method Of Making A Microbead Array With Attached Biomolecules
  • Detection Probe
  • Methods And Compositions To Detect Nucleic Acids In A Biological Sample
  • Compounds And Methods For Enhanced Cellular Uptake
  • Aliquotter System And Workflow
  • Qtls Associated With And Methods For Identifying Lodging Resistance In Soybean
  • Method For Using Probe Based Pcr Detection To Measure The Levels Of Circulating Demethylated Cell Derived Dna As A Measure Of Cell Loss In Diabetes
  • Method And Apparatus For Generating Thermal Melting Curves In A Microfluidic Device
  • Foldable Polymers As Probes
  • Functionalized Nanoparticles And Methods Of Use
  • Method For Detecting Human Papillomavirus Mrna
  • Methods And Systems For Image Processing Of Microfluidic Devices
  • Microrna Compounds And Methods For Modulating Mir-122
  • Direct Amplification And Detection Of Viral And Bacterial Pathogens
  • Aptamer-Based Colorimetric Sensor Systems
  • Nucleic Acid Based Fluorescent Sensor For Copper Detection
  • Digital Amplification
  • Distinguishing Pca3 Messenger Rna Species In Benign And Malignant Prostate Tissues
  • Small Conditional Rnas
  • Use Of Products Of Pcr Amplification Carrying Elements Of Secondary Structure To Improve Pcr-Based Nucleic Acid Detection
  • System And Method Including Thermal Cycler Modules
  • Species-Specific, Genus-Specific And Universal Dna Probes And Amplification Primers To Rapidly Detect And Identify Common Bacterial And Fungal Pathogens And Associated Antibiotic Resistance Genes From Clinical Specimens For Diagnosis In Microbiology Laboratories
  • Multiplex Quantification Of Nucleic Acids In Diseased Cells
  • Methine-Substituted Cyanine Dye Compounds
  • Methods And Systems For Image Processing Of Microfluidic Devices
  • Monitoring Amplification Of Dna During Pcr
  • Method For Detecting Oligonucleotides Using Uv Light Source
  • Compositions And Methods For Cdna Synthesis
  • Method For Detecting And Quantifying Biomaterials By Using Activity Of Nucleic Acid Polymerase Regulated By Target Material
  • Compositions And Methods For Detecting Human Pegivirus 2 (Hpgv-2)
  • Instruments And Methods For Exposing A Receptacle To Multiple Thermal Zones
  • Method Of Detecting Specific Fragments Of Dna Or Rna With The Aid Of A Real-Time Polymerase Chain Reaction
  • Universal Multi-Variant Detection System
  • Distinguishing Pca3 Messenger Rna Species In Benign And Malignant Prostate Tissues
  • Method For Detecting A Target Nucleic Acid Sequence
  • System For Fluorescence Monitoring
  • Method For Analyzing Dynamic Detectable Events At The Single Molecule Level
  • Molecular Counting By Color-Coded Micelles
  • Optical Lens System And Method For Microfluidic Devices
  • Gravity-Assisted Mixing Methods
  • Pna Oligomers, Oligomer Sets, Methods And Kits Pertaining To The Detection Of Bacillus Anthracis
  • Uv Excitable Fluorescent Energy Transfer Dyes
  • Apparatus And Methods For Conducting Assays And High Throughput Screening
  • Assays For Short Sequence Variants
  • Methods And Compositions For The Detection And Analysis Of Nucleic Acids By Signal Amplification
  • Mrna Ratios In Urinary Sediments And/Or Urine As A Prognostic And/Or Theranostic Marker For Prostate Cancer
  • Preprimitive Streak And Mesendoderm Cells
  • Instruments And Methods For Mixing The Contents Of A Detection Chamber
  • Method For Generating A Circularised Nucleic Acid
  • Cyanine Dye Compounds
  • Universal Linker Compositions For The Release Or Transfer Of Chemical Agents From A Polynucleotide
  • Optically Decodable Microcarries, Arrays And Methods
  • Fluorescent Sensor For Mercury
  • Antibody Complexes And Methods For Immunolabeling
  • Definitive Endoderm
  • Particles For Detecting Intracellular Targets
  • Nucleic Acid Based Fluorescent Sensor For Mercury Detection
  • Method For Label-Free Detection Of Hybridized Dna Targets
  • Method For Deblocking Of Labeled Oligonucleotides
  • Molecular Modification Assays
  • Amplification And Detection Reagents For Hiv-1
  • Uv Excitable Energy Transfer Reagents
  • Process For High Throughput Dna Methylation Analysis
  • Charge-Switch Nucleotides
  • Methods For Detection Of A Target Nucleic Acid By Capture Using Multi-Subunit Probes
  • Methods And Kits For Proximity Probing
  • Homogeneous Multiplex Hybridization Analysis By Color And Tm
  • Normalization Control For Hybridization Reactions
  • Methods Of Identifying Nucleic Acid Sequences Using Polycations
  • Arrayed Biomolecules And Their Use In Sequencing
  • Nucleic Acid-Based Detection
  • Detection Probes, Kits And Assays
  • Fluorescent Donor-Acceptor Pair With Low Spectral Overlap
  • Assay Methods And System
  • Methods For Detecting And Identifying Single Molecules
  • Methods, Kits And Compositions Pertaining To Linear Beacons
  • Pcr Method For Nucleic Acid Quantification Utilizing Second Or Third Order Rate Constants
  • Kits And Reaction Mixtures Containing Modified Probe Molecules
  • Oligonucleotides And Analogs Labeled With Energy Transfer Dyes
  • Arrays Of Magnetic Particles
  • Kits Useful For Sequencing Nucleic Acids
  • Cyanine Dye Compounds
  • Microarrays Of Binary Nucleic Acid Probes For Detecting Nucleic Acid Analytes
  • Lafora's Disease Gene
  • Versatile Nucleic Acid Hairpin Motif For Programming Biomolecular Self-Assembly Pathways
  • Fluorescent Imaging Agents
  • Primers And Probes For Detecting Human Papillomavirus And Human Beta Globin Sequences In Test Samples
  • Assay For Rnase H Activity
  • Assay For Rnase H Activity
  • Assay Cartridge With Reaction Well
  • Aliquotter System And Workflow
  • Compounds And Methods For Enhanced Cellular Uptake
  • Foldable Polymers As Probes
  • Method For Detecting Human Papillomavirus Mrna
  • Digital Amplification
  • Analysis, Secure Access To, And Transmission Of Array Images
  • Methods Of Using Fet Labeled Oligonucleotides That Include A 3′-5′ Exonuclease Resistant Quencher Domain And Compositions For Practicing The Same
  • Microrna Compounds And Methods For Modulating Mir-122
  • Direct Amplification And Detection Of Viral And Bacterial Pathogens
  • Methods And Systems For Image Processing Of Microfluidic Devices
  • Use Of Products Of Pcr Amplification Carrying Elements Of Secondary Structure To Improve Pcr-Based Nucleic Acid Detection
  • System And Method Including Thermal Cycler Modules
  • Compositions And Methods For Detecting Small Rnas, And Uses Thereof
  • Dep2 And Its Uses In Major Depressive Disorder And Other Related Disorders
  • Microfluidic Nucleic Acid Analysis
  • Fluorescent Imaging Agents
  • Microfluidic Device With Reaction Sites Configured For Blind Filling
  • Species-Specific, Genus-Specific And Universal Dna Probes And Amplification Primers To Rapidly Detect And Identify Common Bacterial And Fungal Pathogens And Associated Antibiotic Resistance Genes From Clinical Specimens For Diagnosis In Microbiology Laboratories
  • Distinguishing Pca3 Messenger Rna Species In Benign And Malignant Prostate Tissues
  • Method For Using Probe Based Pcr Detection To Measure The Levels Of Circulating Demethylated Cell Derived Dna As A Measure Of Cell Loss In Diabetes
  • Functionalized Nanoparticles And Methods Of Use
  • Sequences And Methods For Detecting Influenza A And Influenza B Virus
  • Androgen Receptor Down-Regulating Agents And Uses Thereof
  • Un-Supported Polymeric Film With Embedded Microbeads
  • Analyte Detection Using A Needle Projection Patch
  • Dark Quenchers For Donor-Acceptor Energy Transfer
  • Multianalyte Molecular Analysis Using Application-Specific Random Particle Arrays
  • Method And Apparatus For Generating Thermal Melting Curves In A Microfluidic Device
  • Primers And Probes For Detecting Human Papillomavirus And Human Beta Globin Sequences In Test Samples
  • Labeling Dye For Detecting Biomolecule, Labeling Kit, And Method For Detecting Biomolecule
  • Compositions, Methods And Kits For Detection Of An Antigen On A Cell And In A Biological Mixture
  • Enzymatic Nucleic Acid Synthesis: Compositions And Methods For Inhibiting Pyrophosphorolysis
  • Peptides And Antiviral Drug
  • Use Of Base-Modified Deoxynucleoside Triphosphates To Improve Nucleic Acid Detection
  • Method For Amplification-Free Nucleic Acid Detection On Optofluidic Chips
  • Signal-On Architecture For Electronic, Oligonucleotide-Based Detectors
  • Container For Carrying Out And Monitoring Biological Processes
  • Method And System For Real Time Quantification And Monitoring Of Nucleic Acid Amplification Using Electroconductive Or Electrochemically Active Labels
  • Method And Materials For The Cooperative Hybridization Of Oligonucleotides
  • Methods And Materials Using Signaling Probes
  • Method To Detect Prostate Cancer In A Sample
  • Microfluidic Device And Method Of Using Same
  • Apparatus And Methods For Conducting Assays And High Throughput Screening
  • Uv Excitable Fluorescent Energy Transfer Dyes
  • Regents Labeled With Energy Transfer Dyes
  • Dark Quenchers For Donor-Acceptor Energy Transfer
  • Use Of Fluorescent Molecular Beacons In The Detection Of Methylated Nucleic Acids
  • Beta 2 Adrenergic Polymorphism Detection
  • Universal Multi-Variant Detection System
  • Method For Enhancing The Association Rates Of Polynucleotides
  • Mri Contrast Agents And High-Throughput Screening By Mri
  • Probe And Method For Detection And Discrimination Of Types And Subtypes Of Influenza Viruses
  • Method For Determining An Allele Profile Of Nucleic Acid
  • Patch Production
  • Polyvalent Rna-Nanoparticle Compositions
  • Hyperthermophilic Polymerase Enabled Proximity Extension Assay
  • Polynucleotide Encoding A Human Myosin-Like Polypeptide Expressed Predominantly In Heart And Muscle
  • High Specificity Primers, Amplification Methods And Kits
  • Quantitating High Titer Samples By Digital Pcr
  • Coating Method
  • Methods For Making Anterior Foregut Endoderm
  • Method For Detecting The Presence Of A Nucleic Acid In A Sample
  • Uv Excitable Fluorescent Energy Transfer Dyes
  • Nucleic Acid Sequences That Can Be Used As Primers And Probes In The Amplification And Detection Of Sars Coronavirus
  • Methods Of Labeling Polynucleotides With Energy Transfer Dyes
  • Magnetic Immuno Digital Pcr Assay
  • Macrocyclic Hopo Chelators
  • Imaging Individual Mrna Molecules Using Multiple Singly Labeled Probes
  • Methods For Disease Detection
  • Compositions Comprising A Linked Acceptor Moiety
  • Molecular Beacons
  • Dark Quenchers, Probes And Other Conjugates Incorporating The Same, And Their Use
  • Fluorescence Based Biosensor
  • Kinase And Phosphatase Assays
  • Methods, Kits And Compositions Pertaining To Linear Beacons
  • Kit For Assessing Mitochondrial Toxicity
  • Compounds And Methods Of Use Thereof For Assaying Lysophospholipase D Activity
  • Free Reactant Use In Nucleic Acid-Templated Synthesis
  • Evolving New Molecular Function
  • Luminescent Macrocyclic Lanthanide Complexes
  • Microfluidic Reaction Apparatus For High Throughput Screening
  • Methods Of Using Fet Labeled Oligonucleotides That Include A 3′-5′ Exonuclease Resistant Quencher Domain And Compositions For Practicing The Same
  • Nucleic Acid Amplification Using Microfluidic Devices
  • Light Emitting Probes
  • Energy Transfer Dyes With Enhanced Fluorescence
  • Zymogenic Nucleic Acid Detection Methods And Related Kits
  • Devices And Methods For Detecting Nucleic Acid Analytes In Samples
  • Primers And Probes For Detection And Discrimination Of Types And Subtypes Of Influenza Viruses
  • Highly Conserved Tuf Genes And Their Use To Generate Probes And Primers For Detection Of Coagulase-Negative Staphylococcus
  • Sample Container Cap With Centrifugation Status Indicator Device
  • Arid1a And Ppp2r1a Mutations In Cancer
  • Method For Reducing Artifacts In Nucleic Acid Amplification
  • Enzymatic Nucleic Acid Synthesis: Compositions And Methods For Inhibiting Pyrophosphorolysis
  • Methods, Kits And Compositions Pertaining To Pna Molecular Beacons
  • Monitoring Hybridization During Pcr Using Sybr™ Green I
  • Membrane-Permeant Peptide Complexes For Medical Imaging, Diagnostics, And Pharmaceutical Therapy
  • Inversion Probes
  • Kinase Assays Using Polycations
  • Nucleic Acid Detection Using Structured Probes
  • Molecular Torches
  • Nucleic Acid Hairpin Probes And Uses Thereof
  • Methods, Kits And Compositions Pertaining To Detection Complexes
  • Method, Kits And Compositions Pertaining To Detection Complexes
  • Molecular Torches
  • Protein Expression Profiling
  • Isoforms Of Human Pregnancy-Associated Protein-E
  • Assay Procedure Using Fluorogenic Tracers
  • Method For Nucleic Acid Amplification That Results In Low Amplification Bias
  • Coating Method
  • Multi-Aptamer-Based, Cell-Specific, One-Step Tumor Cell Detection Assays
  • Immobilized Nucleic Acid Hybridization Reagent And Method
  • Compositions And Methods For Detection And Isolation Of Phosphorylated Molecules
  • Compounds And Methods Of Use Thereof For Assaying Lysophospholipase D Activity
  • Multifunctional Magnetic Nanoparticle Probes For Intracellular Molecular Imaging And Monitoring
  • Use Of An Extraction Control In A Method Of Extracting Nucleic Acids
  • Redox Potential Mediated Carbon Nanotubes Biosensing In Homogeneous Format
  • Sieving Of Nucleic Acid Samples
  • Primer, Primer Set, And Nucleic Acid Amplification Method And Mutation Detection Method Using The Same
  • Microfluidic Nucleic Acid Analysis
  • Pcr Reaction Mixtures And Methods Of Using Same
  • Isothermal Amplification Components And Processes
  • Dark Quenchers For Donor-Acceptor Energy Transfer
  • Nucleic Acid Functionalized Nanoparticles For Therapeutic Applications
  • Methods, Apparatus, And Computer Programs For Verifying The Integrity Of A Probe
  • Methods For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Cyanine Dyes
  • Optimization Of Gene Expression Analysis Using Immobilized Capture Probes
  • Nucleic Acid Enzyme Biosensors For Ions
  • Reaction Mixtures For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Staphylococcus Detection Assays
  • Digital Amplification
  • Arrayed Biomolecules And Their Use In Sequencing
  • Methods And Compositions To Detect Nucleic Acids In A Biological Sample
  • Use Of Rna/Dna Chimeric Primers For Improved Nucleic Acid Amplification Reactions
  • Method For Using Probe Based Pcr Detection To Measure The Levels Of Circulating Demethylated Β Cell Derived Dna As A Measure Of Β Cell Loss In Diabetes
  • Method And Kit For Establishing An In Vitro Prognosis On A Patient Exhibiting Sirs
  • Spatial Sequestration Of Dynamic Nucleic Acid Circuits
  • Self-Assembled Polynucleotide Structure
  • Method For Deblocking Of Labeled Oligonucleotides
  • Kits For Detecting Group B Streptococci
  • System And Method For Fluorescence Monitoring
  • Nested Pcr Employing Degradable Primers
  • Cyanine Compounds And Their Application As Quenching Compounds
  • Optical Lens System And Method For Microfluidic Devices
  • Method And Kit For The Detection Of Nucleic Acids
  • Non-Competitive Co-Amplification Methods
  • Dna Sequencing And Amplification Systems Using Nanoscale Field Effect Sensor Arrays
  • Method For Identifying Polymorphism Of Nucleic Acid Molecules
  • Optical Lens System And Method For Microfluidic Devices
  • Hepatocyte Lineage Cells
  • Method Of Detecting Norwalk-Like Virus (Gi)
  • Direct Amplification And Detection Of Viral And Bacterial Pathogens
  • Method For Cell Identification And Cell Sorting
  • Methods And Compositions For Multiplexed And Ultrasensitive Microrna Detection
  • Aptamer- And Nucleic Acid Enzyme-Based Systems For Simultaneous Detection Of Multiple Analytes
  • Highly Conserved Genes And Their Use To Generate Probes And Primers For Detection Of Microorganisms
  • Method For Discriminating Single Nucleotide Polymorphisms
  • Oligonucleotides Labeled With A Plurality Of Fluorophores
  • Cyanine Dye Compounds
  • Multiplex Quantification Of Nucleic Acids In Diseased Cells
  • Compositions, Methods And Kits For Nucleic Acid Synthesis And Amplification
  • Method To Detect Prostate Cancer In A Sample
  • Methods Of Using Fet Labeled Oligonucleotides That Include A 3′-5′ Exonuclease Resistant Quencher Domain And Compositions For Practicing The Same
  • Hybridization Chain Reaction Amplification For In Situ Imaging
  • Methods And Compositions For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Uv Excitable Fluorescent Energy Transfer Dyes
  • Pca3, Pca3 Genes, And Methods Of Use
  • Microfluidic Particle-Analysis Systems
  • Rapid And Efficient Capture Of Dna From Sample Without Using Cell Lysing Reagent
  • Membrane-Permeant Peptide Complexes For Medical Imaging, Diagnostics, And Pharmaceutical Therapy
  • Membrane-Permeant Peptide Complexes For Medical Imaging, Diagnostics, And Pharmaceutical Therapy
  • Detection Probes, Kits And Assays
  • System And Method For Carrying Out And Monitoring Biological Processes
  • Energy Transfer Dyes With Enhanced Fluorescence
  • Methods And Probes For Detecting A Vancomycin Resistance Gene
  • Detection Of Small Nucleic Acids
  • Fluorescence-Mediated Molecular Tomography
  • Oligonucleotide Conjugates
  • Primer Middle Sequence Interference Pcr Technology
  • Compositions Of Toehold Primer Duplexes And Methods Of Use
  • Primers And Methods For Nucleic Acid Amplification
  • Method And Apparatus For Generating Thermal Melting Curves In A Microfluidic Device
  • Digital Amplification
  • Prostate Cancer Prognostic Compositions And Kits
  • Nucleic Acid Amplification
  • Method For Activating A Nucleic Acid For A Polymerase Reaction
  • Creation Of Functionalized Microparticle Libraries By Oligonucleotide Ligation Or Elongation
  • Polyvalent Rna-Nanoparticle Compositions
  • Isolation Of Living Cells And Preparation Of Cell Lines Based On Detection And Quantification Of Preselected Cellular Ribonucleic Acid Sequences
  • Signal Amplification With Lollipop Probes
  • Systems And Methods For High-Resolution In Vivo Imaging Of Biochemical Activity In A Living Organism
  • Delivery Device
  • Method For Simultaneously Performing Multiple Amplification Reactions
  • Method For Performing An Assay With A Nucleic Acid Present In A Specimen
  • Ultra High-Throughput Opti-Nanopore Dna Readout Platform
  • Microfabricated Structure Having Parallel And Orthogonal Flow Channels Controlled By Row And Column Multiplexors
  • Methods For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Markers Of Definitive Endoderm
  • Nucleic Acid Amplification Using Microfluidic Devices
  • Method For Using Probe Based Pcr Detection To Measure The Levels Of Circulating Demethylated Beta Cell Derived Dna As A Measure Of Beta Cell Loss In Diabetes
  • Method Of Making A Microbead Array With Attached Biomolecules
  • Diagnosis And Treatment Of Alzheimer's Disease
  • Microrna Compounds And Methods For Modulating Mir-122
  • Method For Detecting Pathogens Using Molecular Beacons
  • Hybridization Assay Using Self-Quenching Fluorescence Probe
  • Oligonucleotide Probes Bearing Quenchable Fluorescent Labels, And Methods Of Use Thereof
  • High Specificity Primers, Amplification Methods And Kits
  • Through Bond Energy Transfer In Fluorescent Dyes For Labelling Biological Molecules
  • Molecular Torches
  • Uv Excitable Fluorescent Energy Transfer Dyes
  • Methods And Kits For Nucleic Acid Analysis Using Fluorescence Resonance Energy Transfer
  • Methods Using Exogenous, Internal Controls And Analogue Blocks During Nucleic Acid Amplification
  • Methods, Kits And Compositions Pertaining To Pna Molecular Beacons
  • Homogeneous Nucleotide Amplification And Assay
  • Method For Detecting Point Mutations In Dna Utilizing Fluorescence Energy Transfer
  • Hybridization Assay Using Self-Quenching Fluorescence Probe
  • Zymogenic Nucleic Acid Molecules
  • Methods And Systems For Determining A Baseline During Image Processing
  • Probes And Primers For Detection Of Bacterial Pathogens And Antibiotic Resistance Genes
  • Pkr Activation Via Hybridization Chain Reaction
  • Turn Over Probes And Use Thereof For Nucleic Acid Detection
  • Method For Detecting Human Papillomavirus Mrna
  • Sulfonated Diarylrhodamine Dyes
  • Aptamer Based Colorimetric Sensor Systems
  • Simultaneous Quantification Of Nucleic Acids In Diseased Cells
  • Reagentless, Reusable, Bioelectronic Detectors
  • Molecular Modification Assays
  • Markers Of Definitive Endoderm
  • Assays For Short Sequence Variants
  • Amphiphilic Substances And Functionalized Lipid Vesicles Including The Same
  • Molecular Modification Assays
  • Charge Switch Nucleotides
  • Apparatus And Methods For Conducting Assays And High Throughput Screening
  • Methine-Substituted Cyanine Dye Compounds
  • Centrifuge System And Workflow
  • Patch Production
  • Methods And Systems For Image Processing Of Microfluidic Devices
  • Particles For Detecting Intracellular Targets
  • System And Method Including Multiple Processing Lanes Executing Processing Protocols
  • Reliable Fluorescence Correction Method For Two-Color Measurement Fluorescence System
  • Instruments And Methods For Mixing The Contents Of A Detection Chamber
  • Improved Methods For Cdna Synthesis
  • Detection Method Using Dissociated Rolling Circle Amplification
  • Process For High Throughput Dna Methylation Analysis
  • Chromosome Conformation Capture In Partitions
  • Triggered Rnai
  • Methods Of Analyzing Polynucleotides Employing Energy Transfer Dyes
  • Microfluidic Device And Methods Of Using Same
  • Dark Quenchers For Donor-Acceptor Energy Transfer
  • Multiple Reporter Read-Out For Bioassays
  • Detectably Labeled Dual Conformation Oligonucleotide Probes, Assays And Kits
  • Methods Of Analyzing Polynucleotides Employing Energy Transfer Dyes
  • Triggered Rnai
  • Nonlinear Optical Detection Of Molecules Comprising An Unnatural Amino Acid Possessing A Hyperpolarizability
  • Compositions And Methods For Enhanced Sensitivity And Specificity Of Nucleic Acid Synthesis
  • Optimization Of Gene Expression Analysis Using Immobilized Capture Probes
  • Methods, Kits And Reaction Mixtures For Analyzing Single-Stranded Nucleic Acid Sequences
  • Templated Nanoconjugates
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0396-303

    DOI

    http://dx.doi.org/10.1038/nbt0396-303

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1007118508

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9630890


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0303", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Macromolecular and Materials Chemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Chemical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biotechnology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Primers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fluorescent Dyes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Probes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Structure", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Naphthalenesulfonates", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nucleic Acid Hybridization", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "p-Dimethylaminoazobenzene", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "name": [
                "Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA. sanjay@phri.nyu.edu"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tyagi", 
            "givenName": "S", 
            "id": "sg:person.01327277653.33", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327277653.33"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Kramer", 
            "givenName": "F R", 
            "id": "sg:person.01164065707.84", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164065707.84"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1073/pnas.78.8.4833", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002062184"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-2836(71)90434-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004456995"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0040-4039(00)97099-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005459978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-2836(71)90433-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006146488"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.21.10024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011105937"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/22.4.662", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017896557"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.4.6.357", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022411407"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/22.6.920", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024801233"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/21.16.3761", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028217069"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.88.16.7276", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029593996"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(91)90309-h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033719540"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(89)90473-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037516649"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/13.12.4485", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037525378"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.93.18.9881", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040243126"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.bi.47.070178.004131", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040564866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0993-1026", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045863719", 
              "https://doi.org/10.1038/nbt0993-1026"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.85.23.8790", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049046341"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00063a022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055159349"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00491a022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055178141"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2106161", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062522377"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1996-03", 
        "datePublishedReg": "1996-03-01", 
        "description": "We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization does not occur when the target contains a mismatched nucleotide or a deletion. The probes are particularly suited for monitoring the synthesis of specific nucleic acids in real time. When used in nucleic acid amplification assays, gene detection is homogeneous and sensitive, and can be carried out in a sealed tube. When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt0396-303", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2453141", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2533460", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "14"
          }
        ], 
        "name": "Molecular Beacons: Probes that Fluoresce upon Hybridization", 
        "pagination": "303", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "21e8e33734e6d86bda4ce7a419500bae80f69231ed6745f62d872795ebd77019"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "9630890"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt0396-303"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1007118508"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt0396-303", 
          "https://app.dimensions.ai/details/publication/pub.1007118508"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T18:10", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000442.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nbt0396-303"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0396-303'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0396-303'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0396-303'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0396-303'


     

    This table displays all metadata directly associated to this object as RDF triples.

    179 TRIPLES      21 PREDICATES      59 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt0396-303 schema:about N1d345d04f2a646b3b09729d927c66ed5
    2 N2c719d1abbef4a98be97f811c61f917a
    3 N3bae474ff6474e878fa942f5851575d4
    4 N610f40dbcff543118add0fdc8a118f68
    5 N6426d32336194363800b709880aa73fd
    6 N736eeb5571404b14b9dbf05280270c54
    7 N8edbdfea7c0c42249340829946dbefdf
    8 N9d546d47db3846aa9497c8bda5b36a8c
    9 Nb4fc5fcb67b14cd980db3e26995a4cd2
    10 Nfc62a8814ca643a9ade367e806a18063
    11 anzsrc-for:03
    12 anzsrc-for:0303
    13 schema:author N0eb7b14d199e48b3a1d4bc6eb3563755
    14 schema:citation sg:pub.10.1038/nbt0993-1026
    15 https://doi.org/10.1016/0003-2697(89)90473-9
    16 https://doi.org/10.1016/0003-2697(91)90309-h
    17 https://doi.org/10.1016/0022-2836(71)90433-5
    18 https://doi.org/10.1016/0022-2836(71)90434-7
    19 https://doi.org/10.1016/s0040-4039(00)97099-0
    20 https://doi.org/10.1021/bi00063a022
    21 https://doi.org/10.1021/bi00491a022
    22 https://doi.org/10.1073/pnas.78.8.4833
    23 https://doi.org/10.1073/pnas.85.23.8790
    24 https://doi.org/10.1073/pnas.88.16.7276
    25 https://doi.org/10.1073/pnas.91.21.10024
    26 https://doi.org/10.1073/pnas.93.18.9881
    27 https://doi.org/10.1093/nar/13.12.4485
    28 https://doi.org/10.1093/nar/21.16.3761
    29 https://doi.org/10.1093/nar/22.4.662
    30 https://doi.org/10.1093/nar/22.6.920
    31 https://doi.org/10.1101/gr.4.6.357
    32 https://doi.org/10.1126/science.2106161
    33 https://doi.org/10.1146/annurev.bi.47.070178.004131
    34 schema:datePublished 1996-03
    35 schema:datePublishedReg 1996-03-01
    36 schema:description We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization does not occur when the target contains a mismatched nucleotide or a deletion. The probes are particularly suited for monitoring the synthesis of specific nucleic acids in real time. When used in nucleic acid amplification assays, gene detection is homogeneous and sensitive, and can be carried out in a sealed tube. When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced.
    37 schema:genre research_article
    38 schema:inLanguage en
    39 schema:isAccessibleForFree false
    40 schema:isPartOf N672b456f24634901ab8d7c485af35192
    41 Neaefbbefc2db4fca8ab2f7782968e72d
    42 sg:journal.1115214
    43 schema:name Molecular Beacons: Probes that Fluoresce upon Hybridization
    44 schema:pagination 303
    45 schema:productId N471da9b497784ce58cb991e3086439c4
    46 N473e3f90638a4f9fb7ed464bf8227f2e
    47 N58924519042e42ba812f7428d6b493db
    48 N76562365166b4e2286fdd4728ec4fdf0
    49 N767cb8c105f846469815c2ce04a940e2
    50 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007118508
    51 https://doi.org/10.1038/nbt0396-303
    52 schema:sdDatePublished 2019-04-10T18:10
    53 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    54 schema:sdPublisher N1623debc05544825b6526d8447cfb8c6
    55 schema:url https://www.nature.com/articles/nbt0396-303
    56 sgo:license sg:explorer/license/
    57 sgo:sdDataset articles
    58 rdf:type schema:ScholarlyArticle
    59 N0eb7b14d199e48b3a1d4bc6eb3563755 rdf:first sg:person.01327277653.33
    60 rdf:rest Nc1b4a1ef47774253997a6cb91d43a38e
    61 N1623debc05544825b6526d8447cfb8c6 schema:name Springer Nature - SN SciGraph project
    62 rdf:type schema:Organization
    63 N1d345d04f2a646b3b09729d927c66ed5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    64 schema:name Naphthalenesulfonates
    65 rdf:type schema:DefinedTerm
    66 N2c719d1abbef4a98be97f811c61f917a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    67 schema:name Base Sequence
    68 rdf:type schema:DefinedTerm
    69 N3bae474ff6474e878fa942f5851575d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    70 schema:name Nucleic Acid Hybridization
    71 rdf:type schema:DefinedTerm
    72 N471da9b497784ce58cb991e3086439c4 schema:name nlm_unique_id
    73 schema:value 9604648
    74 rdf:type schema:PropertyValue
    75 N473e3f90638a4f9fb7ed464bf8227f2e schema:name doi
    76 schema:value 10.1038/nbt0396-303
    77 rdf:type schema:PropertyValue
    78 N58924519042e42ba812f7428d6b493db schema:name dimensions_id
    79 schema:value pub.1007118508
    80 rdf:type schema:PropertyValue
    81 N610f40dbcff543118add0fdc8a118f68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Molecular Probes
    83 rdf:type schema:DefinedTerm
    84 N6426d32336194363800b709880aa73fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name DNA Primers
    86 rdf:type schema:DefinedTerm
    87 N672b456f24634901ab8d7c485af35192 schema:issueNumber 3
    88 rdf:type schema:PublicationIssue
    89 N736eeb5571404b14b9dbf05280270c54 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name p-Dimethylaminoazobenzene
    91 rdf:type schema:DefinedTerm
    92 N76562365166b4e2286fdd4728ec4fdf0 schema:name readcube_id
    93 schema:value 21e8e33734e6d86bda4ce7a419500bae80f69231ed6745f62d872795ebd77019
    94 rdf:type schema:PropertyValue
    95 N767cb8c105f846469815c2ce04a940e2 schema:name pubmed_id
    96 schema:value 9630890
    97 rdf:type schema:PropertyValue
    98 N8edbdfea7c0c42249340829946dbefdf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Polymerase Chain Reaction
    100 rdf:type schema:DefinedTerm
    101 N9d546d47db3846aa9497c8bda5b36a8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Molecular Structure
    103 rdf:type schema:DefinedTerm
    104 Nb4fc5fcb67b14cd980db3e26995a4cd2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Fluorescent Dyes
    106 rdf:type schema:DefinedTerm
    107 Nc1b4a1ef47774253997a6cb91d43a38e rdf:first sg:person.01164065707.84
    108 rdf:rest rdf:nil
    109 Nd62c820c3bc4429eaf796df1737e39fe schema:name Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA. sanjay@phri.nyu.edu
    110 rdf:type schema:Organization
    111 Neaefbbefc2db4fca8ab2f7782968e72d schema:volumeNumber 14
    112 rdf:type schema:PublicationVolume
    113 Nfc62a8814ca643a9ade367e806a18063 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Biotechnology
    115 rdf:type schema:DefinedTerm
    116 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
    117 schema:name Chemical Sciences
    118 rdf:type schema:DefinedTerm
    119 anzsrc-for:0303 schema:inDefinedTermSet anzsrc-for:
    120 schema:name Macromolecular and Materials Chemistry
    121 rdf:type schema:DefinedTerm
    122 sg:grant.2453141 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt0396-303
    123 rdf:type schema:MonetaryGrant
    124 sg:grant.2533460 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt0396-303
    125 rdf:type schema:MonetaryGrant
    126 sg:journal.1115214 schema:issn 1087-0156
    127 1546-1696
    128 schema:name Nature Biotechnology
    129 rdf:type schema:Periodical
    130 sg:person.01164065707.84 schema:familyName Kramer
    131 schema:givenName F R
    132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164065707.84
    133 rdf:type schema:Person
    134 sg:person.01327277653.33 schema:affiliation Nd62c820c3bc4429eaf796df1737e39fe
    135 schema:familyName Tyagi
    136 schema:givenName S
    137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327277653.33
    138 rdf:type schema:Person
    139 sg:pub.10.1038/nbt0993-1026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045863719
    140 https://doi.org/10.1038/nbt0993-1026
    141 rdf:type schema:CreativeWork
    142 https://doi.org/10.1016/0003-2697(89)90473-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037516649
    143 rdf:type schema:CreativeWork
    144 https://doi.org/10.1016/0003-2697(91)90309-h schema:sameAs https://app.dimensions.ai/details/publication/pub.1033719540
    145 rdf:type schema:CreativeWork
    146 https://doi.org/10.1016/0022-2836(71)90433-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006146488
    147 rdf:type schema:CreativeWork
    148 https://doi.org/10.1016/0022-2836(71)90434-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004456995
    149 rdf:type schema:CreativeWork
    150 https://doi.org/10.1016/s0040-4039(00)97099-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005459978
    151 rdf:type schema:CreativeWork
    152 https://doi.org/10.1021/bi00063a022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055159349
    153 rdf:type schema:CreativeWork
    154 https://doi.org/10.1021/bi00491a022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055178141
    155 rdf:type schema:CreativeWork
    156 https://doi.org/10.1073/pnas.78.8.4833 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002062184
    157 rdf:type schema:CreativeWork
    158 https://doi.org/10.1073/pnas.85.23.8790 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049046341
    159 rdf:type schema:CreativeWork
    160 https://doi.org/10.1073/pnas.88.16.7276 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029593996
    161 rdf:type schema:CreativeWork
    162 https://doi.org/10.1073/pnas.91.21.10024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011105937
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1073/pnas.93.18.9881 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040243126
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1093/nar/13.12.4485 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037525378
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1093/nar/21.16.3761 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028217069
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1093/nar/22.4.662 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017896557
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1093/nar/22.6.920 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024801233
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1101/gr.4.6.357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022411407
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1126/science.2106161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062522377
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1146/annurev.bi.47.070178.004131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040564866
    179 rdf:type schema:CreativeWork
     




    Preview window. Press ESC to close (or click here)


    ...