The Genetic Engineering of Two Commercial Potato Cultivars for Resistance to Potato Virus X View Full Text


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Article Info

DATE

1989-03

AUTHORS

André Hoekema, Marianne J. Huisman, Lucy Molendijk, Peter J. M. van den Elzen, Ben J. C. Cornelissen

ABSTRACT

We efficiently transformed the commercial cultivars of potato (Solanum tuberosum) Bintje, Desiree and Escort after optimizing the conditions for regeneration from potato tuber discs. For transformation, tuber discs were cocultivated with Agrobacterium tumefaciens using a disarmed binary vector system. This system allowed the introduction of a chimaeric gene encoding the coat protein (CP) of potato virus X (PVX) into two cultivars most susceptible to this virus, Bintje and Escort. Five transgenic plant lines with expression levels of CP higher than 0.1% of soluble leaf protein were analyzed for resistance to a challenging inoculation with PVX (1 μg/ml). We observed a delay in symptom development as well as a drastic reduction in the accumulation of virus. Furthermore, we found a correlation between the expression level of the CP–gene and the reduction in virus accumulation. Cytogenetic analysis of 62 independently obtained transgenic lines showed the normal tetraploid number of chromosomes (2n = 4x = 48) in 97 percent of the examined plants. Phenotypically all these plants appeared normal. One plant line exhibited an abnormal phenotype and contained about the octaploid number of chromosomes (4n ≈ 8x = 96). These results and preliminary data on morphological characteristics, as these are determined in the official variety registration procedure, indicate that potato cultivars can be genetically engineered to contain new desirable traits with preservation of their intrinsic properties. More... »

PAGES

273

References to SciGraph publications

  • 1986-04. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 1987-09. Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium in THEORETICAL AND APPLIED GENETICS
  • 1985-12. Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens in NATURE
  • 1982-09. Chromosome variation in protoplast-derived potato plants in THEORETICAL AND APPLIED GENETICS
  • 1986-05. Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants in NATURE
  • 1983-07. From tumour to tuber; tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv. ‘Maris Bard’) in THEORETICAL AND APPLIED GENETICS
  • 1988-01. Introduction of foreign genes into potato cultivars Bintje and Désirée using an Agrobacterium tumefaciens binary vector in PLANT CELL REPORTS
  • 1988-05. Viral Protection in Transgenic Tobacco Plants Expressing the Cucumber Mosaic Virus Coat Protein or its Antisense RNA in NATURE BIOTECHNOLOGY
  • 1983-05. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid in NATURE
  • 1987-07. Efficient Transfer of a Glyphosate Tolerance Gene into Tomato Using a Binary Agrobacterium Tumefaciens Vector in NATURE BIOTECHNOLOGY
  • 1988-01. A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors in PLANT CELL REPORTS
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 1985-07. T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato in PLANT MOLECULAR BIOLOGY
  • 1983-07. A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation in NATURE
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    http://scigraph.springernature.com/pub.10.1038/nbt0389-273

    DOI

    http://dx.doi.org/10.1038/nbt0389-273

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