Development of New Cloning Vectors for the Production of Immunogenic Outer Membrane Fusion Proteins in Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-02

AUTHORS

Pierre Cornells, Javier Cote Sierra, Antonio Lim, Achut Malur, Sumalee Tungpradabkul, Hamida Tazka, Alexandre Leitão, Carlos V. Martins, Christi di Perna, Lea Brys, Patrick De Baetselier, Raymond Hamers

ABSTRACT

The Pseudomonas aeruginosa lipoprotein gene (oprI) was modified by cloning an in-frame polylinker in both orientations at the end of oprI. The resulting plasmids pVUB1 and pVUB2 allow high lipoprotein production in E. coli after IPTG induction. The modified lipoproteins are present in the outer membrane and surface-exposed. Outer membrane-bound fusion proteins of different sizes were produced and used to generate antibodies without use of adjuvant. An 87 bp DNA fragment from the vp72 capsid protein gene of African Swine Fever virus (ASFV) and the entire Leishmania major glycoprotein gp63 gene were expressed in this system. Finally, a fusion lipoprotein containing a 16 amino acid epitope from the pre-S2b region of Hepatitis B virus (HBV) was presented by an antigen-presenting cell line to a T-cell hybridoma while the corresponding cross-linked S2b peptide was not. The results suggest that OprI-based fusion proteins can be used to generate both humoral and cellular immune responses. More... »

PAGES

203

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0296-203

DOI

http://dx.doi.org/10.1038/nbt0296-203

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046748148

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9636324


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