Development of New Cloning Vectors for the Production of Immunogenic Outer Membrane Fusion Proteins in Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-02

AUTHORS

Pierre Cornells, Javier Cote Sierra, Antonio Lim, Achut Malur, Sumalee Tungpradabkul, Hamida Tazka, Alexandre Leitão, Carlos V. Martins, Christi di Perna, Lea Brys, Patrick De Baetselier, Raymond Hamers

ABSTRACT

The Pseudomonas aeruginosa lipoprotein gene (oprI) was modified by cloning an in-frame polylinker in both orientations at the end of oprI. The resulting plasmids pVUB1 and pVUB2 allow high lipoprotein production in E. coli after IPTG induction. The modified lipoproteins are present in the outer membrane and surface-exposed. Outer membrane-bound fusion proteins of different sizes were produced and used to generate antibodies without use of adjuvant. An 87 bp DNA fragment from the vp72 capsid protein gene of African Swine Fever virus (ASFV) and the entire Leishmania major glycoprotein gp63 gene were expressed in this system. Finally, a fusion lipoprotein containing a 16 amino acid epitope from the pre-S2b region of Hepatitis B virus (HBV) was presented by an antigen-presenting cell line to a T-cell hybridoma while the corresponding cross-linked S2b peptide was not. The results suggest that OprI-based fusion proteins can be used to generate both humoral and cellular immune responses. More... »

PAGES

203

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0296-203

DOI

http://dx.doi.org/10.1038/nbt0296-203

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046748148

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9636324


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "African Swine Fever Virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Outer Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Capsid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lipoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vaccines, Synthetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Algemene Biologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cornells", 
        "givenName": "Pierre", 
        "id": "sg:person.013467544177.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013467544177.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Algemene Biologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sierra", 
        "givenName": "Javier Cote", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Algemene Biologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lim", 
        "givenName": "Antonio", 
        "id": "sg:person.0770771451.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770771451.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Algemene Biologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Malur", 
        "givenName": "Achut", 
        "id": "sg:person.01132577441.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132577441.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Algemene Biologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tungpradabkul", 
        "givenName": "Sumalee", 
        "id": "sg:person.01164661163.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164661163.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Algemene Biologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tazka", 
        "givenName": "Hamida", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto de Investiga\u00e7\u00e3o Cient\u00edfica Tropical", 
          "id": "https://www.grid.ac/institutes/grid.420930.c", 
          "name": [
            "Laboratorio de Doen\u00e7as Infecciosas, Faculdade de Medicina Veterinaria, Rua Gomes Freire, 1199 Lisboa, Portugal.", 
            "CVZ Instituto Investiga\u00e7\u00e3o Cientif\u00edca Tropical, Lisboa, Portugal."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Leit\u00e3o", 
        "givenName": "Alexandre", 
        "id": "sg:person.01330134311.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330134311.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Laboratorio de Doen\u00e7as Infecciosas, Faculdade de Medicina Veterinaria, Rua Gomes Freire, 1199 Lisboa, Portugal."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Martins", 
        "givenName": "Carlos V.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Cellulaire Immunologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "di Perna", 
        "givenName": "Christi", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Cellulaire Immunologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brys", 
        "givenName": "Lea", 
        "id": "sg:person.01366516661.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366516661.87"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "Laboratorium Cellulaire Immunologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium."
          ], 
          "type": "Organization"
        }, 
        "familyName": "De Baetselier", 
        "givenName": "Patrick", 
        "id": "sg:person.01050356110.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050356110.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Laboratorio de Doen\u00e7as Infecciosas, Faculdade de Medicina Veterinaria, Rua Gomes Freire, 1199 Lisboa, Portugal."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hamers", 
        "givenName": "Raymond", 
        "id": "sg:person.010147743024.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010147743024.47"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00763177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001169941", 
          "https://doi.org/10.1007/bf00763177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/eji.1830201234", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001810959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1989.tb00187.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002636227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-2478(94)90052-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003928029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-2478(94)90052-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003928029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-7799(93)90067-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007288629"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00633832", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011442977", 
          "https://doi.org/10.1007/bf00633832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00633832", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011442977", 
          "https://doi.org/10.1007/bf00633832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.76.9.4350", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011704795"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00583678", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015697036", 
          "https://doi.org/10.1007/bf00583678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1291-1369", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016865959", 
          "https://doi.org/10.1038/nbt1291-1369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.13.6127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017209386"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci115461", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017613867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/0-89603-239-6:407", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022984309", 
          "https://doi.org/10.1385/0-89603-239-6:407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(83)90197-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023191886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(83)90197-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023191886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1990.tb00705.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024590872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/342561a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024893323", 
          "https://doi.org/10.1038/342561a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.74.12.5463", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025360556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01316896", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027819415", 
          "https://doi.org/10.1007/bf01316896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01316896", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027819415", 
          "https://doi.org/10.1007/bf01316896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0495-366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031929455", 
          "https://doi.org/10.1038/nbt0495-366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0166-6851(93)90106-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035207327"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0166-6851(93)90106-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035207327"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(90)90432-q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037380996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(82)90593-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037588156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0493-491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038687091", 
          "https://doi.org/10.1038/nbt0493-491"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.mi.37.100183.002233", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040430693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.74.4.1417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041068873"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(87)90275-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042228089"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(87)90275-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042228089"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(87)90441-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046527882"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(87)90441-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046527882"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.78.6.3824", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047301083"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-7799(93)90068-k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049823492"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/vim.1987.1.177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059326216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/6.4.449", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059980870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-139-9-2215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060368883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2468182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062538915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.168.1.140-151.1986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062715173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1986.tb04602.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078824881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079804887", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081705208", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082361988", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1995.tb07342.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082441039"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-02", 
    "datePublishedReg": "1996-02-01", 
    "description": "The Pseudomonas aeruginosa lipoprotein gene (oprI) was modified by cloning an in-frame polylinker in both orientations at the end of oprI. The resulting plasmids pVUB1 and pVUB2 allow high lipoprotein production in E. coli after IPTG induction. The modified lipoproteins are present in the outer membrane and surface-exposed. Outer membrane-bound fusion proteins of different sizes were produced and used to generate antibodies without use of adjuvant. An 87 bp DNA fragment from the vp72 capsid protein gene of African Swine Fever virus (ASFV) and the entire Leishmania major glycoprotein gp63 gene were expressed in this system. Finally, a fusion lipoprotein containing a 16 amino acid epitope from the pre-S2b region of Hepatitis B virus (HBV) was presented by an antigen-presenting cell line to a T-cell hybridoma while the corresponding cross-linked S2b peptide was not. The results suggest that OprI-based fusion proteins can be used to generate both humoral and cellular immune responses.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nbt0296-203", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1094195", 
        "issn": [
          "0733-222X"
        ], 
        "name": "Bio/Technology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "14"
      }
    ], 
    "name": "Development of New Cloning Vectors for the Production of Immunogenic Outer Membrane Fusion Proteins in Escherichia coli", 
    "pagination": "203", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "deb08b7a3b3a6d3b65caf62f06d68e18047c257aa8513812927f06195791c4e2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9636324"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8309273"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nbt0296-203"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046748148"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nbt0296-203", 
      "https://app.dimensions.ai/details/publication/pub.1046748148"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T21:26", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000442.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nbt0296-203"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0296-203'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0296-203'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0296-203'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0296-203'


 

This table displays all metadata directly associated to this object as RDF triples.

338 TRIPLES      21 PREDICATES      84 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nbt0296-203 schema:about N2f605a7530cb40a0ad80f4ec792724b0
2 N3374759d87ce4fa4aaec267e9adb7180
3 N37abc596d96a44119115a65096c925b0
4 N5013a2d9c05b435f8739693d8d935f3f
5 N53251ce63ff6469ab3533c89ff5c9d88
6 N6450490f6cc0424592407fe134bf2470
7 N6a14f7dfed9644f99ca3c3082760d3fe
8 N6e1df1281a434083bb10a9e1ac6400b0
9 N6fb91758afe04c86946e4051868556ef
10 N7acb206348b849c18894ca74ce0ec6b9
11 N8013db1e6f684523a9b576401927bab3
12 N883b1a7edfeb4858922881754f6a7a26
13 N96c95d279ffb48669392f2283291ece2
14 N9ae8b93395d34627ac6208963a4f8ce8
15 Ndeea209067b948298c964c99ab710ba8
16 Nec107978950c4c8aba81556bf26b8f13
17 anzsrc-for:11
18 anzsrc-for:1107
19 schema:author N21b0d1a1706b40f1a7ab7e63beb00cc2
20 schema:citation sg:pub.10.1007/bf00583678
21 sg:pub.10.1007/bf00633832
22 sg:pub.10.1007/bf00763177
23 sg:pub.10.1007/bf01316896
24 sg:pub.10.1038/227680a0
25 sg:pub.10.1038/342561a0
26 sg:pub.10.1038/nbt0493-491
27 sg:pub.10.1038/nbt0495-366
28 sg:pub.10.1038/nbt1291-1369
29 sg:pub.10.1385/0-89603-239-6:407
30 https://app.dimensions.ai/details/publication/pub.1079804887
31 https://app.dimensions.ai/details/publication/pub.1081705208
32 https://app.dimensions.ai/details/publication/pub.1082361988
33 https://doi.org/10.1002/eji.1830201234
34 https://doi.org/10.1002/j.1460-2075.1986.tb04602.x
35 https://doi.org/10.1002/j.1460-2075.1995.tb07342.x
36 https://doi.org/10.1016/0003-2697(82)90593-0
37 https://doi.org/10.1016/0022-1759(87)90441-8
38 https://doi.org/10.1016/0042-6822(90)90432-q
39 https://doi.org/10.1016/0165-2478(94)90052-3
40 https://doi.org/10.1016/0166-6851(93)90106-8
41 https://doi.org/10.1016/0167-7799(93)90067-j
42 https://doi.org/10.1016/0167-7799(93)90068-k
43 https://doi.org/10.1016/0378-1119(83)90197-x
44 https://doi.org/10.1016/0378-1119(87)90275-7
45 https://doi.org/10.1073/pnas.74.12.5463
46 https://doi.org/10.1073/pnas.74.4.1417
47 https://doi.org/10.1073/pnas.76.9.4350
48 https://doi.org/10.1073/pnas.78.6.3824
49 https://doi.org/10.1089/vim.1987.1.177
50 https://doi.org/10.1093/nar/16.13.6127
51 https://doi.org/10.1093/protein/6.4.449
52 https://doi.org/10.1099/00221287-139-9-2215
53 https://doi.org/10.1111/j.1365-2958.1989.tb00187.x
54 https://doi.org/10.1111/j.1365-2958.1990.tb00705.x
55 https://doi.org/10.1126/science.2468182
56 https://doi.org/10.1128/jb.168.1.140-151.1986
57 https://doi.org/10.1146/annurev.mi.37.100183.002233
58 https://doi.org/10.1172/jci115461
59 schema:datePublished 1996-02
60 schema:datePublishedReg 1996-02-01
61 schema:description The Pseudomonas aeruginosa lipoprotein gene (oprI) was modified by cloning an in-frame polylinker in both orientations at the end of oprI. The resulting plasmids pVUB1 and pVUB2 allow high lipoprotein production in E. coli after IPTG induction. The modified lipoproteins are present in the outer membrane and surface-exposed. Outer membrane-bound fusion proteins of different sizes were produced and used to generate antibodies without use of adjuvant. An 87 bp DNA fragment from the vp72 capsid protein gene of African Swine Fever virus (ASFV) and the entire Leishmania major glycoprotein gp63 gene were expressed in this system. Finally, a fusion lipoprotein containing a 16 amino acid epitope from the pre-S2b region of Hepatitis B virus (HBV) was presented by an antigen-presenting cell line to a T-cell hybridoma while the corresponding cross-linked S2b peptide was not. The results suggest that OprI-based fusion proteins can be used to generate both humoral and cellular immune responses.
62 schema:genre research_article
63 schema:inLanguage en
64 schema:isAccessibleForFree false
65 schema:isPartOf N83ca00f8e2ae45579dad983d9f22f3b7
66 Nc64db42a1da9431cbfd3a1660276a9a6
67 sg:journal.1094195
68 schema:name Development of New Cloning Vectors for the Production of Immunogenic Outer Membrane Fusion Proteins in Escherichia coli
69 schema:pagination 203
70 schema:productId N34d89cffcfec400b87e5f0154efffa46
71 N5d0a6f6846104e099c8e1ac36118bea2
72 N8ea3bb146bdc46658e06c2cfc44c4b84
73 N9e0ce56512e643a4bb23da6f7d46e780
74 Nddc530d1484a40edbad8112b1847b1eb
75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046748148
76 https://doi.org/10.1038/nbt0296-203
77 schema:sdDatePublished 2019-04-10T21:26
78 schema:sdLicense https://scigraph.springernature.com/explorer/license/
79 schema:sdPublisher N2d6504602222483d9ad81d9af2f82296
80 schema:url https://www.nature.com/articles/nbt0296-203
81 sgo:license sg:explorer/license/
82 sgo:sdDataset articles
83 rdf:type schema:ScholarlyArticle
84 N21b0d1a1706b40f1a7ab7e63beb00cc2 rdf:first sg:person.013467544177.16
85 rdf:rest Naa4cb3bdb0574dc092c32cdbd4693c58
86 N2d6504602222483d9ad81d9af2f82296 schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N2f605a7530cb40a0ad80f4ec792724b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name African Swine Fever Virus
90 rdf:type schema:DefinedTerm
91 N3374759d87ce4fa4aaec267e9adb7180 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Animals
93 rdf:type schema:DefinedTerm
94 N34d89cffcfec400b87e5f0154efffa46 schema:name nlm_unique_id
95 schema:value 8309273
96 rdf:type schema:PropertyValue
97 N37abc596d96a44119115a65096c925b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Escherichia coli
99 rdf:type schema:DefinedTerm
100 N40b43efbb9494a05a7b91954146d33b4 schema:affiliation N7d5e525719a7435399cd27b4ab890610
101 schema:familyName Martins
102 schema:givenName Carlos V.
103 rdf:type schema:Person
104 N4c8ae2cce19242a3a817e7e415f5b904 rdf:first sg:person.0770771451.45
105 rdf:rest Nedcbfde5fb9544f2983f9de85faaf4cd
106 N5013a2d9c05b435f8739693d8d935f3f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Lipoproteins
108 rdf:type schema:DefinedTerm
109 N50527a7e02f44d048077142f67bbf565 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
110 schema:familyName Tazka
111 schema:givenName Hamida
112 rdf:type schema:Person
113 N53251ce63ff6469ab3533c89ff5c9d88 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Genes, Bacterial
115 rdf:type schema:DefinedTerm
116 N5d0a6f6846104e099c8e1ac36118bea2 schema:name readcube_id
117 schema:value deb08b7a3b3a6d3b65caf62f06d68e18047c257aa8513812927f06195791c4e2
118 rdf:type schema:PropertyValue
119 N6450490f6cc0424592407fe134bf2470 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Cloning, Molecular
121 rdf:type schema:DefinedTerm
122 N6a14f7dfed9644f99ca3c3082760d3fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Vaccines, Synthetic
124 rdf:type schema:DefinedTerm
125 N6ab00eb3890f4f8c8e4734280972cbc8 rdf:first sg:person.01366516661.87
126 rdf:rest N6d83968de2ef49aaa4bdb6c9b8ae23b7
127 N6d83968de2ef49aaa4bdb6c9b8ae23b7 rdf:first sg:person.01050356110.11
128 rdf:rest Ncabe2f8fadc6425ab616d4e635a7472f
129 N6e1df1281a434083bb10a9e1ac6400b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Base Sequence
131 rdf:type schema:DefinedTerm
132 N6fb91758afe04c86946e4051868556ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Bacterial Outer Membrane Proteins
134 rdf:type schema:DefinedTerm
135 N6ffa3c906b24404190cc46224720126a rdf:first sg:person.01164661163.83
136 rdf:rest Ne3d65c00e3ab4d459153af276a432fdf
137 N7acb206348b849c18894ca74ce0ec6b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Recombinant Fusion Proteins
139 rdf:type schema:DefinedTerm
140 N7b6137c44d1a4a738dff3bc2562451a7 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
141 schema:familyName Sierra
142 schema:givenName Javier Cote
143 rdf:type schema:Person
144 N7d5e525719a7435399cd27b4ab890610 schema:name Laboratorio de Doenças Infecciosas, Faculdade de Medicina Veterinaria, Rua Gomes Freire, 1199 Lisboa, Portugal.
145 rdf:type schema:Organization
146 N8013db1e6f684523a9b576401927bab3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Molecular Sequence Data
148 rdf:type schema:DefinedTerm
149 N83ca00f8e2ae45579dad983d9f22f3b7 schema:issueNumber 2
150 rdf:type schema:PublicationIssue
151 N877f468b1ad74ddebffd22fcc2639747 rdf:first sg:person.01330134311.60
152 rdf:rest N955e89a0ea524b3ba244086e8c65be33
153 N883b1a7edfeb4858922881754f6a7a26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Amino Acid Sequence
155 rdf:type schema:DefinedTerm
156 N8ea3bb146bdc46658e06c2cfc44c4b84 schema:name doi
157 schema:value 10.1038/nbt0296-203
158 rdf:type schema:PropertyValue
159 N955e89a0ea524b3ba244086e8c65be33 rdf:first N40b43efbb9494a05a7b91954146d33b4
160 rdf:rest Nfd37dfcc3319484b97e55993090099d1
161 N96c95d279ffb48669392f2283291ece2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Capsid
163 rdf:type schema:DefinedTerm
164 N9ae8b93395d34627ac6208963a4f8ce8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Protein Engineering
166 rdf:type schema:DefinedTerm
167 N9e0ce56512e643a4bb23da6f7d46e780 schema:name dimensions_id
168 schema:value pub.1046748148
169 rdf:type schema:PropertyValue
170 Naa4cb3bdb0574dc092c32cdbd4693c58 rdf:first N7b6137c44d1a4a738dff3bc2562451a7
171 rdf:rest N4c8ae2cce19242a3a817e7e415f5b904
172 Nc64db42a1da9431cbfd3a1660276a9a6 schema:volumeNumber 14
173 rdf:type schema:PublicationVolume
174 Nc7afd6f23c0e4671865c1bc93949426b schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
175 schema:familyName di Perna
176 schema:givenName Christi
177 rdf:type schema:Person
178 Ncabe2f8fadc6425ab616d4e635a7472f rdf:first sg:person.010147743024.47
179 rdf:rest rdf:nil
180 Nddc530d1484a40edbad8112b1847b1eb schema:name pubmed_id
181 schema:value 9636324
182 rdf:type schema:PropertyValue
183 Ndeea209067b948298c964c99ab710ba8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Bacterial Proteins
185 rdf:type schema:DefinedTerm
186 Ne3d65c00e3ab4d459153af276a432fdf rdf:first N50527a7e02f44d048077142f67bbf565
187 rdf:rest N877f468b1ad74ddebffd22fcc2639747
188 Nec107978950c4c8aba81556bf26b8f13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Genetic Vectors
190 rdf:type schema:DefinedTerm
191 Nedcbfde5fb9544f2983f9de85faaf4cd rdf:first sg:person.01132577441.31
192 rdf:rest N6ffa3c906b24404190cc46224720126a
193 Nf2f4357637334fe5bdd024c757240242 schema:name Laboratorio de Doenças Infecciosas, Faculdade de Medicina Veterinaria, Rua Gomes Freire, 1199 Lisboa, Portugal.
194 rdf:type schema:Organization
195 Nfd37dfcc3319484b97e55993090099d1 rdf:first Nc7afd6f23c0e4671865c1bc93949426b
196 rdf:rest N6ab00eb3890f4f8c8e4734280972cbc8
197 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
198 schema:name Medical and Health Sciences
199 rdf:type schema:DefinedTerm
200 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
201 schema:name Immunology
202 rdf:type schema:DefinedTerm
203 sg:journal.1094195 schema:issn 0733-222X
204 schema:name Bio/Technology
205 rdf:type schema:Periodical
206 sg:person.010147743024.47 schema:affiliation Nf2f4357637334fe5bdd024c757240242
207 schema:familyName Hamers
208 schema:givenName Raymond
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010147743024.47
210 rdf:type schema:Person
211 sg:person.01050356110.11 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
212 schema:familyName De Baetselier
213 schema:givenName Patrick
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050356110.11
215 rdf:type schema:Person
216 sg:person.01132577441.31 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
217 schema:familyName Malur
218 schema:givenName Achut
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132577441.31
220 rdf:type schema:Person
221 sg:person.01164661163.83 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
222 schema:familyName Tungpradabkul
223 schema:givenName Sumalee
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164661163.83
225 rdf:type schema:Person
226 sg:person.01330134311.60 schema:affiliation https://www.grid.ac/institutes/grid.420930.c
227 schema:familyName Leitão
228 schema:givenName Alexandre
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330134311.60
230 rdf:type schema:Person
231 sg:person.013467544177.16 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
232 schema:familyName Cornells
233 schema:givenName Pierre
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013467544177.16
235 rdf:type schema:Person
236 sg:person.01366516661.87 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
237 schema:familyName Brys
238 schema:givenName Lea
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366516661.87
240 rdf:type schema:Person
241 sg:person.0770771451.45 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
242 schema:familyName Lim
243 schema:givenName Antonio
244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770771451.45
245 rdf:type schema:Person
246 sg:pub.10.1007/bf00583678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015697036
247 https://doi.org/10.1007/bf00583678
248 rdf:type schema:CreativeWork
249 sg:pub.10.1007/bf00633832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011442977
250 https://doi.org/10.1007/bf00633832
251 rdf:type schema:CreativeWork
252 sg:pub.10.1007/bf00763177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001169941
253 https://doi.org/10.1007/bf00763177
254 rdf:type schema:CreativeWork
255 sg:pub.10.1007/bf01316896 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027819415
256 https://doi.org/10.1007/bf01316896
257 rdf:type schema:CreativeWork
258 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
259 https://doi.org/10.1038/227680a0
260 rdf:type schema:CreativeWork
261 sg:pub.10.1038/342561a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024893323
262 https://doi.org/10.1038/342561a0
263 rdf:type schema:CreativeWork
264 sg:pub.10.1038/nbt0493-491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038687091
265 https://doi.org/10.1038/nbt0493-491
266 rdf:type schema:CreativeWork
267 sg:pub.10.1038/nbt0495-366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031929455
268 https://doi.org/10.1038/nbt0495-366
269 rdf:type schema:CreativeWork
270 sg:pub.10.1038/nbt1291-1369 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016865959
271 https://doi.org/10.1038/nbt1291-1369
272 rdf:type schema:CreativeWork
273 sg:pub.10.1385/0-89603-239-6:407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022984309
274 https://doi.org/10.1385/0-89603-239-6:407
275 rdf:type schema:CreativeWork
276 https://app.dimensions.ai/details/publication/pub.1079804887 schema:CreativeWork
277 https://app.dimensions.ai/details/publication/pub.1081705208 schema:CreativeWork
278 https://app.dimensions.ai/details/publication/pub.1082361988 schema:CreativeWork
279 https://doi.org/10.1002/eji.1830201234 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001810959
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1002/j.1460-2075.1986.tb04602.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078824881
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1002/j.1460-2075.1995.tb07342.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082441039
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1016/0003-2697(82)90593-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037588156
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1016/0022-1759(87)90441-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046527882
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1016/0042-6822(90)90432-q schema:sameAs https://app.dimensions.ai/details/publication/pub.1037380996
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1016/0165-2478(94)90052-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003928029
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1016/0166-6851(93)90106-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035207327
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1016/0167-7799(93)90067-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1007288629
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1016/0167-7799(93)90068-k schema:sameAs https://app.dimensions.ai/details/publication/pub.1049823492
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1016/0378-1119(83)90197-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023191886
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1016/0378-1119(87)90275-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042228089
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1073/pnas.74.12.5463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025360556
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1073/pnas.74.4.1417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041068873
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1073/pnas.76.9.4350 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011704795
308 rdf:type schema:CreativeWork
309 https://doi.org/10.1073/pnas.78.6.3824 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047301083
310 rdf:type schema:CreativeWork
311 https://doi.org/10.1089/vim.1987.1.177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059326216
312 rdf:type schema:CreativeWork
313 https://doi.org/10.1093/nar/16.13.6127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017209386
314 rdf:type schema:CreativeWork
315 https://doi.org/10.1093/protein/6.4.449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059980870
316 rdf:type schema:CreativeWork
317 https://doi.org/10.1099/00221287-139-9-2215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060368883
318 rdf:type schema:CreativeWork
319 https://doi.org/10.1111/j.1365-2958.1989.tb00187.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002636227
320 rdf:type schema:CreativeWork
321 https://doi.org/10.1111/j.1365-2958.1990.tb00705.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024590872
322 rdf:type schema:CreativeWork
323 https://doi.org/10.1126/science.2468182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062538915
324 rdf:type schema:CreativeWork
325 https://doi.org/10.1128/jb.168.1.140-151.1986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062715173
326 rdf:type schema:CreativeWork
327 https://doi.org/10.1146/annurev.mi.37.100183.002233 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040430693
328 rdf:type schema:CreativeWork
329 https://doi.org/10.1172/jci115461 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017613867
330 rdf:type schema:CreativeWork
331 https://www.grid.ac/institutes/grid.11486.3a schema:alternateName Flanders Institute for Biotechnology
332 schema:name Laboratorium Algemene Biologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium.
333 Laboratorium Cellulaire Immunologie, Vrije Universiteit Brussel, Vlaams Instituut Biotechnologie, Paardenstraat 65, B-1640 St. Genesius Rode, Belgium.
334 rdf:type schema:Organization
335 https://www.grid.ac/institutes/grid.420930.c schema:alternateName Instituto de Investigação Científica Tropical
336 schema:name CVZ Instituto Investigação Cientifíca Tropical, Lisboa, Portugal.
337 Laboratorio de Doenças Infecciosas, Faculdade de Medicina Veterinaria, Rua Gomes Freire, 1199 Lisboa, Portugal.
338 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...