Red-Shifted Excitation Mutants of the Green Fluorescent Protein View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-02

AUTHORS

Simon Delagrave, Rachael E. Hawtin, Christopher M. Silva, Mary M. Yang, Douglas C. Youvan

ABSTRACT

Using optimized combinatorial mutagenesis techniques and Digital Imaging Spectroscopy (DIS), we have isolated mutants of the cloned Aequorea victoria green fluorescent protein (GFP) that show red-shifted excitation spectra similar to that of Renilla reniformis GFP. Selective excitation of wild-type versus Red-Shifted GFP (RSGFP) enables spectral separation of these proteins. Six contiguous codons spanning the tyrosine chromophore region were randomized and sequence analysis of the mutants revealed a tyrosineglycine consensus. These mutants will enable the simultaneous analysis of two promoters or proteins per cell or organism. In consideration of the multitude of applications which are developing for GFP alone, we envisage that spectrally shifted fluorescent proteins will be of value to a diversity of research programs, including developmental and cell biology, drug-screening, and diagnostic assays. More... »

PAGES

151

References to SciGraph publications

Journal

TITLE

Bio/Technology

ISSUE

2

VOLUME

13

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0295-151

    DOI

    http://dx.doi.org/10.1038/nbt0295-151

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030498390

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9634755


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