Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-02-01

AUTHORS

Michael G. Koziel, Gary L. Beland, Cindy Bowman, Nadine B. Carozzi, Rebecca Crenshaw, Lyle Crossland, John Dawson, Nalini Desai, Martha Hill, Sue Kadwell, Karen Launis, Kelly Lewis, Daryl Maddox, Kathryn McPherson, Moez R. Meghji, Ellis Merlin, Richard Rhodes, Gregory W. Warren, Martha Wright, Stephen V. Evola

ABSTRACT

We introduced a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis into immature embryos of an elite line of maize using microprojectile bombardment. This gene was expressed using either the CaMV 35S promoter or a combination of two tissue specific promoters derived from maize. High levels of CryIA(b) protein were obtained using both promoter configurations. Hybrid maize plants resulting from crosses of transgenic elite inbred plants with commercial inbred lines were evaluated for resistance to European corn borer under field conditions. Plants expressing high levels of the insecticidal protein exhibited excellent resistance to repeated heavy infestations of this pest. More... »

PAGES

194-200

Journal

TITLE

Nature Biotechnology

ISSUE

2

VOLUME

11

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0293-194

    DOI

    http://dx.doi.org/10.1038/nbt0293-194

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034075899


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