Kluyveromyces as a Host for Heterologous Gene Expression: Expression and Secretion of Prochymosin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-02

AUTHORS

Johan A. van den Berg, Kees J. van der Laken, Albert J. J. van Ooyen, Ton C. H. M. Renniers, Krijn Rietveld, Albert Schaap, Anthony J. Brake, Robert J. Bishop, Kathleen Schultz, Donna Moyer, Michael Richman, Jeffrey R. Shuster

ABSTRACT

We have developed the yeast Kluyveromyces lactis as a host organism for the production of the milk-clotting enzyme chymosin. In contrast to Saccharomyces cerevisiae, we found that this yeast is capable of the synthesis and secretion of fully active prochymosin. Various signal sequences could be used to efficiently direct the secretion of prochymosin in Kluyveromyces, but not in S. cerevisiae. We conclude that the efficient synthetic and secretory capacity of this heterologous protein is a property of the yeast Kluyveromyces. These results have led to the development of a large scale production process for chymosin. More... »

PAGES

135

References to SciGraph publications

Journal

TITLE

Bio/Technology

ISSUE

2

VOLUME

8

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0290-135

    DOI

    http://dx.doi.org/10.1038/nbt0290-135

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043314865

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1366557


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