Isolation and Purification of Protein Granules from Escherichia coli Cells Overproducing Bovine Growth Hormone View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-02

AUTHORS

Ronald G. Schoner, Lee F. Ellis, Brigitte E. Schoner

ABSTRACT

High-level expression of bovine growth hormone in Escherichia coli results in the formation of distinct cytoplasmic granules that are visible with the phase-contrast microscope. These granules were examined by transmission and scanning electron microscopy. Intact granules were isolated from crude cell lysates by differential centrifugation and were further purified by a simple washing procedure that yields nearly homogeneous bovine growth hormone. More... »

PAGES

151

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0285-151

DOI

http://dx.doi.org/10.1038/nbt0285-151

DIMENSIONS

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