Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-01

AUTHORS

A de Saizieu, U Certa, J Warrington, C Gray, W Keck, J Mous

ABSTRACT

We have used high-density oligonucleotide probe arrays (chips) for bacterial transcript imaging. We designed a chip containing probes representing 106 Hemophilus influenzae genes and 100 Streptococcus pneumoniae genes. The apparent lack of polyadenylated transcripts excludes enrichment of mRNA by affinity purification and we thus used total, chemically biotinylated RNA as hybridization probe. We show that hybridization of Streptococcus RNA to a chip allows simultaneous quantification of the transcript levels. The sensitivity was found to be in the range of one to five transcripts per cell. The quantitative chip results were in good agreement with conventional Northern blot analysis of selected genes. This technology allows simultaneous and quantitative measurement of the transcriptional activity of entire bacterial genomes on a single oligonucleotide probe array. More... »

PAGES

45

Journal

TITLE

Nature Biotechnology

ISSUE

1

VOLUME

16

Author Affiliations

Clinical Trials linked to this publication

Related Patents

  • Functionalized Silicon Compounds And Methods For Their Synthesis And Use
  • Functionalized Silicon Compounds And Methods For Their Synthesis And Use
  • Inib, Inia And Inic Genes Of Mycobacteria And Methods Of Use
  • Methods Of Genetic Analysis Using Nucleic Acid Arrays
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • Methods And Systems For Digitally Counting Features On Arrays
  • Computer Method And System For Correlating Data
  • Methods For Array Preparation Using Substrate Rotation
  • Functionalized Silicon Compounds And Methods For Their Synthesis And Use
  • Methods For Constructing Subarrays And Subarrays Made Thereby
  • Functionalized Silicon Compounds And Methods For Their Synthesis And Use
  • Photoactivatable Silane Compounds And Methods For Their Synthesis And Use
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • Device For Chemical And Biochemical Reactions Using Photo-Generated Reagents
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • Apparatus And Methods For Arraying Solution Onto A Solid Support
  • Microarray Printing Device Including Printing Pins With Flat Tips And Exterior Channel And Method Of Manufacture
  • Polymeric Arrays And Methods For Their Use In Binding Assays
  • Tag Nucleic Acids And Probe Arrays
  • Methods And Compositions For Amplifying Detectable Signals In Specific Binding Assays
  • Porous Silica Substrates For Polymer Synthesis And Assays
  • Methods For Improved Array Preparation
  • Spatially Addressable Molecular Barcoding
  • Device For Chemical And Biochemical Reactions Using Photo-Generated Reagents
  • Methods And Compositions For Amplifying Detectable Signals In Specific Binding Assays
  • Spatially Addressable Molecular Barcoding
  • Phosphite Ester Oxidation In Nucleic Acid Array Preparation
  • Methods For Selecting Nucleic Acid Probes
  • Use Of Hexahydrolupulones As Antibacterial Agents
  • Functionalized Silicon Compounds And Methods For Their Synthesis And Use
  • Methods Of Enhancing Functional Performance Of Nucleic Acid Arrays
  • Amplification And Other Enzymatic Reactions Performed On Nucleic Acid Arrays
  • Functionalized Silicon Compounds And Methods For Their Synthesis And Use
  • Polymeric Arrays And Methods For Their Use In Binding Assays
  • Device For Chemical And Biochemical Reactions Using Photo-Generated Reagents
  • Massively Parallel Single Cell Analysis
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • Method Of Differential Display Of Prokaryotic Messenger Rna By Rtpcr
  • Functionalized Silicon Compounds And Methods For Their Synthesis And Use
  • Polymeric Arrays And Methods For Their Use In Binding Assays
  • Massively Parallel Single Cell Analysis
  • Functionalized Silicon Compounds And Methods For Their Synthesis And Use
  • Method And Apparatus For Chemical And Biochemical Reactions Using Photo-Generated Reagents
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0198-45

    DOI

    http://dx.doi.org/10.1038/nbt0198-45

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035355418

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9447592


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biotinylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blotting, Northern", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fluorescent Dyes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Haemophilus influenzae", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nucleic Acid Hybridization", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oligonucleotide Probes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Streptococcus pneumoniae", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcription, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Roche (Switzerland)", 
              "id": "https://www.grid.ac/institutes/grid.417570.0", 
              "name": [
                "Pharma Division, F. Hoffmann-La Roche Ltd., Basel, Switzerland."
              ], 
              "type": "Organization"
            }, 
            "familyName": "de Saizieu", 
            "givenName": "A", 
            "id": "sg:person.01114143254.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114143254.39"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Certa", 
            "givenName": "U", 
            "id": "sg:person.01163441175.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163441175.70"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Warrington", 
            "givenName": "J", 
            "type": "Person"
          }, 
          {
            "familyName": "Gray", 
            "givenName": "C", 
            "type": "Person"
          }, 
          {
            "familyName": "Keck", 
            "givenName": "W", 
            "id": "sg:person.0752732600.97", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752732600.97"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Mous", 
            "givenName": "J", 
            "id": "sg:person.01031536543.81", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031536543.81"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1046/j.1365-2958.1997.2481617.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006473624"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-2958.1997.2481617.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006473624"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-2958.1998.00668.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016943861"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/25.9.1830", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021682560"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.92.24.11140", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027843462"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.6.7.639", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028833356"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.bi.54.070185.005443", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032202016"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0076-6879(90)84321-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047194161"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.11.5022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050061899"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1354393", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062477287"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.270.5235.467", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062551475"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.8430319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062655559"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.8430319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062655559"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.8430319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062655559"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.175.7.2026-2036.1993", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062722519"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082391202", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1998-01", 
        "datePublishedReg": "1998-01-01", 
        "description": "We have used high-density oligonucleotide probe arrays (chips) for bacterial transcript imaging. We designed a chip containing probes representing 106 Hemophilus influenzae genes and 100 Streptococcus pneumoniae genes. The apparent lack of polyadenylated transcripts excludes enrichment of mRNA by affinity purification and we thus used total, chemically biotinylated RNA as hybridization probe. We show that hybridization of Streptococcus RNA to a chip allows simultaneous quantification of the transcript levels. The sensitivity was found to be in the range of one to five transcripts per cell. The quantitative chip results were in good agreement with conventional Northern blot analysis of selected genes. This technology allows simultaneous and quantitative measurement of the transcriptional activity of entire bacterial genomes on a single oligonucleotide probe array.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt0198-45", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "16"
          }
        ], 
        "name": "Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays", 
        "pagination": "45", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "a86b213a218b313732faeca24303d569bd03a13f003b1c2cfb9ad537cd81693d"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "9447592"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt0198-45"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1035355418"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt0198-45", 
          "https://app.dimensions.ai/details/publication/pub.1035355418"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T13:01", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000444.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nbt0198-45"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0198-45'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0198-45'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0198-45'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0198-45'


     

    This table displays all metadata directly associated to this object as RDF triples.

    175 TRIPLES      21 PREDICATES      52 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt0198-45 schema:about N01c1a5986bcf4fd180b12fa7f4dab826
    2 N452a9dc5ab8448aa98bd6bf52a2e776b
    3 N58aa75c8a21340aab06926b143f20997
    4 N6476eb1d772e47baac3e1fe0c79d84b0
    5 N6c08c396f9c142f9986ab5978fac6827
    6 N7294ecb9e3204ba286249c1c9256bcb5
    7 N80f80aa4659b4883a8ae27eae81d2fc4
    8 Nb89e6d9d1a214abfb9b064e6f7ca8563
    9 Nbb056f7438bf42f09a5eebf6782b8c1e
    10 Ndea55a97a6ca4797ab942c3843abbca2
    11 anzsrc-for:06
    12 anzsrc-for:0604
    13 schema:author N8c569b90b18842649a0e4dd28c0edcc1
    14 schema:citation https://app.dimensions.ai/details/publication/pub.1082391202
    15 https://doi.org/10.1016/0076-6879(90)84321-7
    16 https://doi.org/10.1046/j.1365-2958.1997.2481617.x
    17 https://doi.org/10.1046/j.1365-2958.1998.00668.x
    18 https://doi.org/10.1073/pnas.91.11.5022
    19 https://doi.org/10.1073/pnas.92.24.11140
    20 https://doi.org/10.1093/nar/25.9.1830
    21 https://doi.org/10.1101/gr.6.7.639
    22 https://doi.org/10.1126/science.1354393
    23 https://doi.org/10.1126/science.270.5235.467
    24 https://doi.org/10.1126/science.8430319
    25 https://doi.org/10.1128/jb.175.7.2026-2036.1993
    26 https://doi.org/10.1146/annurev.bi.54.070185.005443
    27 schema:datePublished 1998-01
    28 schema:datePublishedReg 1998-01-01
    29 schema:description We have used high-density oligonucleotide probe arrays (chips) for bacterial transcript imaging. We designed a chip containing probes representing 106 Hemophilus influenzae genes and 100 Streptococcus pneumoniae genes. The apparent lack of polyadenylated transcripts excludes enrichment of mRNA by affinity purification and we thus used total, chemically biotinylated RNA as hybridization probe. We show that hybridization of Streptococcus RNA to a chip allows simultaneous quantification of the transcript levels. The sensitivity was found to be in the range of one to five transcripts per cell. The quantitative chip results were in good agreement with conventional Northern blot analysis of selected genes. This technology allows simultaneous and quantitative measurement of the transcriptional activity of entire bacterial genomes on a single oligonucleotide probe array.
    30 schema:genre research_article
    31 schema:inLanguage en
    32 schema:isAccessibleForFree false
    33 schema:isPartOf N3162c5682a104378ab667cd85e2afd5a
    34 N6063154a60f74e7886424c6d7ff05f3e
    35 sg:journal.1115214
    36 schema:name Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays
    37 schema:pagination 45
    38 schema:productId N2ef4ffcb069a4759af52e6a74ee186a2
    39 N331c483e08264bab9491eab6f9ea03c3
    40 N5ffab8c484e14530bccefd02e2ac875f
    41 Nbd56bb1bc7c742be89f16aaf430aad0c
    42 Nd70c9d070c8446b7b5e5a6c871c95383
    43 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035355418
    44 https://doi.org/10.1038/nbt0198-45
    45 schema:sdDatePublished 2019-04-10T13:01
    46 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    47 schema:sdPublisher N2a82759a11af4517bca447c09dd7d41e
    48 schema:url https://www.nature.com/articles/nbt0198-45
    49 sgo:license sg:explorer/license/
    50 sgo:sdDataset articles
    51 rdf:type schema:ScholarlyArticle
    52 N01c1a5986bcf4fd180b12fa7f4dab826 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    53 schema:name Haemophilus influenzae
    54 rdf:type schema:DefinedTerm
    55 N1307a2aa59f541dca8cb3ea3045edaf3 rdf:first sg:person.01163441175.70
    56 rdf:rest N8a04136d4c6042208116df49189ab82f
    57 N2a82759a11af4517bca447c09dd7d41e schema:name Springer Nature - SN SciGraph project
    58 rdf:type schema:Organization
    59 N2ef4ffcb069a4759af52e6a74ee186a2 schema:name doi
    60 schema:value 10.1038/nbt0198-45
    61 rdf:type schema:PropertyValue
    62 N3162c5682a104378ab667cd85e2afd5a schema:volumeNumber 16
    63 rdf:type schema:PublicationVolume
    64 N331c483e08264bab9491eab6f9ea03c3 schema:name nlm_unique_id
    65 schema:value 9604648
    66 rdf:type schema:PropertyValue
    67 N452a9dc5ab8448aa98bd6bf52a2e776b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    68 schema:name Genome, Bacterial
    69 rdf:type schema:DefinedTerm
    70 N58aa75c8a21340aab06926b143f20997 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    71 schema:name RNA, Bacterial
    72 rdf:type schema:DefinedTerm
    73 N5ffab8c484e14530bccefd02e2ac875f schema:name pubmed_id
    74 schema:value 9447592
    75 rdf:type schema:PropertyValue
    76 N6063154a60f74e7886424c6d7ff05f3e schema:issueNumber 1
    77 rdf:type schema:PublicationIssue
    78 N6476eb1d772e47baac3e1fe0c79d84b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Oligonucleotide Probes
    80 rdf:type schema:DefinedTerm
    81 N67f806ab1a704b81a5785e3bb1b0cb9b schema:familyName Gray
    82 schema:givenName C
    83 rdf:type schema:Person
    84 N6c08c396f9c142f9986ab5978fac6827 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name Biotinylation
    86 rdf:type schema:DefinedTerm
    87 N7294ecb9e3204ba286249c1c9256bcb5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Streptococcus pneumoniae
    89 rdf:type schema:DefinedTerm
    90 N80f80aa4659b4883a8ae27eae81d2fc4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Fluorescent Dyes
    92 rdf:type schema:DefinedTerm
    93 N8a04136d4c6042208116df49189ab82f rdf:first Ne17fff15cc6a43be966877ac1fa4bc76
    94 rdf:rest N9fa8830146c84c17b66c5b63405784fb
    95 N8c1034171f9b43dd8d588ef858f830f0 rdf:first sg:person.0752732600.97
    96 rdf:rest Nfbbbf9d4f7ad4417a287047e10389cf0
    97 N8c569b90b18842649a0e4dd28c0edcc1 rdf:first sg:person.01114143254.39
    98 rdf:rest N1307a2aa59f541dca8cb3ea3045edaf3
    99 N9fa8830146c84c17b66c5b63405784fb rdf:first N67f806ab1a704b81a5785e3bb1b0cb9b
    100 rdf:rest N8c1034171f9b43dd8d588ef858f830f0
    101 Nb89e6d9d1a214abfb9b064e6f7ca8563 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Transcription, Genetic
    103 rdf:type schema:DefinedTerm
    104 Nbb056f7438bf42f09a5eebf6782b8c1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Nucleic Acid Hybridization
    106 rdf:type schema:DefinedTerm
    107 Nbd56bb1bc7c742be89f16aaf430aad0c schema:name dimensions_id
    108 schema:value pub.1035355418
    109 rdf:type schema:PropertyValue
    110 Nd70c9d070c8446b7b5e5a6c871c95383 schema:name readcube_id
    111 schema:value a86b213a218b313732faeca24303d569bd03a13f003b1c2cfb9ad537cd81693d
    112 rdf:type schema:PropertyValue
    113 Ndea55a97a6ca4797ab942c3843abbca2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Blotting, Northern
    115 rdf:type schema:DefinedTerm
    116 Ne17fff15cc6a43be966877ac1fa4bc76 schema:familyName Warrington
    117 schema:givenName J
    118 rdf:type schema:Person
    119 Nfbbbf9d4f7ad4417a287047e10389cf0 rdf:first sg:person.01031536543.81
    120 rdf:rest rdf:nil
    121 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    122 schema:name Biological Sciences
    123 rdf:type schema:DefinedTerm
    124 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    125 schema:name Genetics
    126 rdf:type schema:DefinedTerm
    127 sg:journal.1115214 schema:issn 1087-0156
    128 1546-1696
    129 schema:name Nature Biotechnology
    130 rdf:type schema:Periodical
    131 sg:person.01031536543.81 schema:familyName Mous
    132 schema:givenName J
    133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031536543.81
    134 rdf:type schema:Person
    135 sg:person.01114143254.39 schema:affiliation https://www.grid.ac/institutes/grid.417570.0
    136 schema:familyName de Saizieu
    137 schema:givenName A
    138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114143254.39
    139 rdf:type schema:Person
    140 sg:person.01163441175.70 schema:familyName Certa
    141 schema:givenName U
    142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163441175.70
    143 rdf:type schema:Person
    144 sg:person.0752732600.97 schema:familyName Keck
    145 schema:givenName W
    146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752732600.97
    147 rdf:type schema:Person
    148 https://app.dimensions.ai/details/publication/pub.1082391202 schema:CreativeWork
    149 https://doi.org/10.1016/0076-6879(90)84321-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047194161
    150 rdf:type schema:CreativeWork
    151 https://doi.org/10.1046/j.1365-2958.1997.2481617.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006473624
    152 rdf:type schema:CreativeWork
    153 https://doi.org/10.1046/j.1365-2958.1998.00668.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016943861
    154 rdf:type schema:CreativeWork
    155 https://doi.org/10.1073/pnas.91.11.5022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050061899
    156 rdf:type schema:CreativeWork
    157 https://doi.org/10.1073/pnas.92.24.11140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027843462
    158 rdf:type schema:CreativeWork
    159 https://doi.org/10.1093/nar/25.9.1830 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021682560
    160 rdf:type schema:CreativeWork
    161 https://doi.org/10.1101/gr.6.7.639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028833356
    162 rdf:type schema:CreativeWork
    163 https://doi.org/10.1126/science.1354393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062477287
    164 rdf:type schema:CreativeWork
    165 https://doi.org/10.1126/science.270.5235.467 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062551475
    166 rdf:type schema:CreativeWork
    167 https://doi.org/10.1126/science.8430319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655559
    168 rdf:type schema:CreativeWork
    169 https://doi.org/10.1128/jb.175.7.2026-2036.1993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062722519
    170 rdf:type schema:CreativeWork
    171 https://doi.org/10.1146/annurev.bi.54.070185.005443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032202016
    172 rdf:type schema:CreativeWork
    173 https://www.grid.ac/institutes/grid.417570.0 schema:alternateName Roche (Switzerland)
    174 schema:name Pharma Division, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
    175 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...