Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-01

AUTHORS

George Georgiou, Christos Stathopoulos, Patrick S. Daugherty, Amiya R. Nayak, Brent L. Iverson, Roy Curtiss

ABSTRACT

In recent years there has been considerable progress towards the development of expression systems for the display of heterologous polypeptides and, to a lesser extent, oligosaccharides on the surface of bacteria or yeast. The availability of protein display vectors has in turn provided the impetus for a range of exciting technologies. Polypeptide libraries can be displayed in bacteria and screened by cell sorting techniques, thus simplifying the isolation of proteins with high affinity for ligands. Expression of antigens on the surface of nonvirulent microorganisms is an attractive approach to the development of high-efficacy recombinant live vaccines. Finally, cells displaying protein receptors or antibodies are of use for analytical applications and bioseparations. More... »

PAGES

29

Journal

TITLE

Nature Biotechnology

ISSUE

1

VOLUME

15

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt0197-29

    DOI

    http://dx.doi.org/10.1038/nbt0197-29

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9035102


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