Homologous Expression and Purification of Mutants of an Essential Protein by Reverse Epitope-Tagging View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-01

AUTHORS

Hans-Ulrich Thomann, Michael Ibba, Kwang-Won Hong, Dieter Söll

ABSTRACT

Purification of mutant enzymes is a prime requirement of biophysical and biochemical studies. Our investigations on the essential Escherichia coli enzyme glutaminyl-tRNA synthetase demand mutant enzymes free of any wild-type protein contamination. However, as it is not possible to express noncomplementing mutant enzymes in an E. coli glnS-deletion strain, we developed a novel strategy to address these problems. Instead of following the common tactic of epitope-tagging the mutant protein of interest on an extrachromosomal genetic element, we fused a reporter epitope to the 5' end of the chromosomal glnS-gene copy: this is referred to as 'reverse epitope-tagging.' The corresponding strain, E. coli HAPPY101, displays a normal phenotype, and glutaminyl-tRNA synthetase is exclusively present as an epitope-tagged form in cell-free extracts. Here we report the use of E. coli HAPPY101 to express and purify a number of mutant glutaminyl-tRNA synthetases independently of their enzymatic activity. In this process, epitope-tagged wild-type protein is readily separated from mutant enzymes by conventional chromatographic methods. In addition, the absence of wild-type can be monitored by immunodetection using a monoclonal antibody specific for the epitope. The strategy described here for expression and purification of an essential enzyme is not restricted to glutaminyl-tRNA synthetase and should be applicable to any essential enzyme that retains sufficient activity to sustain growth following reverse epitope-tagging. More... »

PAGES

50

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0196-50

DOI

http://dx.doi.org/10.1038/nbt0196-50

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047184038

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9636312


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