Homologous Expression and Purification of Mutants of an Essential Protein by Reverse Epitope-Tagging View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-01

AUTHORS

Hans-Ulrich Thomann, Michael Ibba, Kwang-Won Hong, Dieter Söll

ABSTRACT

Purification of mutant enzymes is a prime requirement of biophysical and biochemical studies. Our investigations on the essential Escherichia coli enzyme glutaminyl-tRNA synthetase demand mutant enzymes free of any wild-type protein contamination. However, as it is not possible to express noncomplementing mutant enzymes in an E. coli glnS-deletion strain, we developed a novel strategy to address these problems. Instead of following the common tactic of epitope-tagging the mutant protein of interest on an extrachromosomal genetic element, we fused a reporter epitope to the 5' end of the chromosomal glnS-gene copy: this is referred to as 'reverse epitope-tagging.' The corresponding strain, E. coli HAPPY101, displays a normal phenotype, and glutaminyl-tRNA synthetase is exclusively present as an epitope-tagged form in cell-free extracts. Here we report the use of E. coli HAPPY101 to express and purify a number of mutant glutaminyl-tRNA synthetases independently of their enzymatic activity. In this process, epitope-tagged wild-type protein is readily separated from mutant enzymes by conventional chromatographic methods. In addition, the absence of wild-type can be monitored by immunodetection using a monoclonal antibody specific for the epitope. The strategy described here for expression and purification of an essential enzyme is not restricted to glutaminyl-tRNA synthetase and should be applicable to any essential enzyme that retains sufficient activity to sustain growth following reverse epitope-tagging. More... »

PAGES

50

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0196-50

DOI

http://dx.doi.org/10.1038/nbt0196-50

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047184038

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9636312


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acyl-tRNA Synthetases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Monoclonal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibody Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Artificial Gene Fusion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosome Deletion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epitopes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Nucleic Acid", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Present address Genome Therapeutics Corp., 100 Beaver Street, Waltham, MA 02154."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thomann", 
        "givenName": "Hans-Ulrich", 
        "id": "sg:person.01276144100.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276144100.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ibba", 
        "givenName": "Michael", 
        "id": "sg:person.01270013225.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270013225.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hong", 
        "givenName": "Kwang-Won", 
        "id": "sg:person.01371027336.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371027336.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yale University", 
          "id": "https://www.grid.ac/institutes/grid.47100.32", 
          "name": [
            "Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511."
          ], 
          "type": "Organization"
        }, 
        "familyName": "S\u00f6ll", 
        "givenName": "Dieter", 
        "id": "sg:person.012067245622.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012067245622.45"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nbt1088-1204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001334427", 
          "https://doi.org/10.1038/nbt1088-1204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(85)13011-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001552528"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00331173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001762846", 
          "https://doi.org/10.1007/bf00331173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.8.5.2159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003447716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15257779408012173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014022332"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-0248(88)90312-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018166970"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-0248(88)90312-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018166970"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90558-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022539367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90558-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022539367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(91)90740-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026149744"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.2.488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026991187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0968-0004(00)89045-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034825303"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(90)85004-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035004964"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/347203a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036757994", 
          "https://doi.org/10.1038/347203a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.17.5681", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039128689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.80.16.4949", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039287430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.9.12.5387", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039878546"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(87)90086-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042213289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1989.tb00197.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043421435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(84)90412-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048251785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.3.968", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050973501"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(89)90257-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051883881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(89)90257-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051883881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/352213a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052831152", 
          "https://doi.org/10.1038/352213a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00004a001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055156739"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00015a038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055157286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00239a009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055166600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/2.4.293", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059980419"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7701322", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062648974"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.173.8.2633-2638.1991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062720352"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079492588", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081662991", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1993.tb06215.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082390200"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-01", 
    "datePublishedReg": "1996-01-01", 
    "description": "Purification of mutant enzymes is a prime requirement of biophysical and biochemical studies. Our investigations on the essential Escherichia coli enzyme glutaminyl-tRNA synthetase demand mutant enzymes free of any wild-type protein contamination. However, as it is not possible to express noncomplementing mutant enzymes in an E. coli glnS-deletion strain, we developed a novel strategy to address these problems. Instead of following the common tactic of epitope-tagging the mutant protein of interest on an extrachromosomal genetic element, we fused a reporter epitope to the 5' end of the chromosomal glnS-gene copy: this is referred to as 'reverse epitope-tagging.' The corresponding strain, E. coli HAPPY101, displays a normal phenotype, and glutaminyl-tRNA synthetase is exclusively present as an epitope-tagged form in cell-free extracts. Here we report the use of E. coli HAPPY101 to express and purify a number of mutant glutaminyl-tRNA synthetases independently of their enzymatic activity. In this process, epitope-tagged wild-type protein is readily separated from mutant enzymes by conventional chromatographic methods. In addition, the absence of wild-type can be monitored by immunodetection using a monoclonal antibody specific for the epitope. The strategy described here for expression and purification of an essential enzyme is not restricted to glutaminyl-tRNA synthetase and should be applicable to any essential enzyme that retains sufficient activity to sustain growth following reverse epitope-tagging.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nbt0196-50", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1094195", 
        "issn": [
          "0733-222X"
        ], 
        "name": "Bio/Technology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "14"
      }
    ], 
    "name": "Homologous Expression and Purification of Mutants of an Essential Protein by Reverse Epitope-Tagging", 
    "pagination": "50", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e0c4c347b618b45d9599f336600a089c439d934f3b41d68b5129ab142b39580a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9636312"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8309273"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nbt0196-50"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1047184038"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nbt0196-50", 
      "https://app.dimensions.ai/details/publication/pub.1047184038"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000442.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nbt0196-50"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0196-50'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0196-50'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0196-50'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0196-50'


 

This table displays all metadata directly associated to this object as RDF triples.

227 TRIPLES      21 PREDICATES      70 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nbt0196-50 schema:about N0e9346a667ff4703a6c1db85da113b65
2 N104a9e6ff5154520b0986daab86a7c93
3 N23886cb4e9214dcb8046c916a39856bd
4 N46d89a46d9f94ed9aaa08cea43984bf5
5 N4e695d27eaef40bba3959be9f7f99fce
6 N561f2c9e4a8c4591a4a4e46fc6f60d8c
7 N678a7caebdf54638840eb6462b7942c6
8 N8e4a649196cc48e58a5647bcc404bcf9
9 Na9a705b49fbd40ca86e4b51ddfdcaaea
10 Nfc6d166814904a9fa95145b2ecc5f3b3
11 Nfe84ba5a238b495794ed317ef2d33845
12 anzsrc-for:06
13 anzsrc-for:0601
14 schema:author Ned13928637214afd8a847982612a2cb3
15 schema:citation sg:pub.10.1007/bf00331173
16 sg:pub.10.1038/347203a0
17 sg:pub.10.1038/352213a0
18 sg:pub.10.1038/nbt1088-1204
19 https://app.dimensions.ai/details/publication/pub.1079492588
20 https://app.dimensions.ai/details/publication/pub.1081662991
21 https://doi.org/10.1002/j.1460-2075.1993.tb06215.x
22 https://doi.org/10.1016/0022-0248(88)90312-0
23 https://doi.org/10.1016/0022-2836(91)90740-w
24 https://doi.org/10.1016/0076-6879(90)85004-8
25 https://doi.org/10.1016/0092-8674(84)90412-4
26 https://doi.org/10.1016/0092-8674(87)90086-9
27 https://doi.org/10.1016/0378-1119(88)90558-6
28 https://doi.org/10.1016/0378-1119(89)90257-6
29 https://doi.org/10.1016/s0076-6879(85)13011-9
30 https://doi.org/10.1016/s0968-0004(00)89045-3
31 https://doi.org/10.1021/bi00004a001
32 https://doi.org/10.1021/bi00015a038
33 https://doi.org/10.1021/bi00239a009
34 https://doi.org/10.1073/pnas.80.16.4949
35 https://doi.org/10.1073/pnas.82.17.5681
36 https://doi.org/10.1073/pnas.82.2.488
37 https://doi.org/10.1073/pnas.86.3.968
38 https://doi.org/10.1080/15257779408012173
39 https://doi.org/10.1093/protein/2.4.293
40 https://doi.org/10.1111/j.1365-2958.1989.tb00197.x
41 https://doi.org/10.1126/science.7701322
42 https://doi.org/10.1128/jb.173.8.2633-2638.1991
43 https://doi.org/10.1128/mcb.8.5.2159
44 https://doi.org/10.1128/mcb.9.12.5387
45 schema:datePublished 1996-01
46 schema:datePublishedReg 1996-01-01
47 schema:description Purification of mutant enzymes is a prime requirement of biophysical and biochemical studies. Our investigations on the essential Escherichia coli enzyme glutaminyl-tRNA synthetase demand mutant enzymes free of any wild-type protein contamination. However, as it is not possible to express noncomplementing mutant enzymes in an E. coli glnS-deletion strain, we developed a novel strategy to address these problems. Instead of following the common tactic of epitope-tagging the mutant protein of interest on an extrachromosomal genetic element, we fused a reporter epitope to the 5' end of the chromosomal glnS-gene copy: this is referred to as 'reverse epitope-tagging.' The corresponding strain, E. coli HAPPY101, displays a normal phenotype, and glutaminyl-tRNA synthetase is exclusively present as an epitope-tagged form in cell-free extracts. Here we report the use of E. coli HAPPY101 to express and purify a number of mutant glutaminyl-tRNA synthetases independently of their enzymatic activity. In this process, epitope-tagged wild-type protein is readily separated from mutant enzymes by conventional chromatographic methods. In addition, the absence of wild-type can be monitored by immunodetection using a monoclonal antibody specific for the epitope. The strategy described here for expression and purification of an essential enzyme is not restricted to glutaminyl-tRNA synthetase and should be applicable to any essential enzyme that retains sufficient activity to sustain growth following reverse epitope-tagging.
48 schema:genre research_article
49 schema:inLanguage en
50 schema:isAccessibleForFree false
51 schema:isPartOf N4e0349809d214f71bd99c51f7e383f44
52 N8d6894ae4f3d4e8cb632cab6841f2a75
53 sg:journal.1094195
54 schema:name Homologous Expression and Purification of Mutants of an Essential Protein by Reverse Epitope-Tagging
55 schema:pagination 50
56 schema:productId N225104354fa944f7a55bdb23129c046a
57 N273bd6099ec147b5aff8b4c1ecfd3c13
58 N341da7edc5c94006938649fdecc7eee8
59 N361ac95fcaaf41f2bf7776e95938cda4
60 N93c2b1cdecaa451c864969b07aea8773
61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047184038
62 https://doi.org/10.1038/nbt0196-50
63 schema:sdDatePublished 2019-04-10T18:10
64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
65 schema:sdPublisher N69fd0afd29e945ebb0359eca1e268986
66 schema:url https://www.nature.com/articles/nbt0196-50
67 sgo:license sg:explorer/license/
68 sgo:sdDataset articles
69 rdf:type schema:ScholarlyArticle
70 N0e9346a667ff4703a6c1db85da113b65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Sequence Homology, Nucleic Acid
72 rdf:type schema:DefinedTerm
73 N104a9e6ff5154520b0986daab86a7c93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Escherichia coli
75 rdf:type schema:DefinedTerm
76 N225104354fa944f7a55bdb23129c046a schema:name readcube_id
77 schema:value e0c4c347b618b45d9599f336600a089c439d934f3b41d68b5129ab142b39580a
78 rdf:type schema:PropertyValue
79 N23886cb4e9214dcb8046c916a39856bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Epitopes
81 rdf:type schema:DefinedTerm
82 N273bd6099ec147b5aff8b4c1ecfd3c13 schema:name dimensions_id
83 schema:value pub.1047184038
84 rdf:type schema:PropertyValue
85 N27fe0109681441a4862cbed5864ac9f4 schema:name Present address Genome Therapeutics Corp., 100 Beaver Street, Waltham, MA 02154.
86 rdf:type schema:Organization
87 N341da7edc5c94006938649fdecc7eee8 schema:name doi
88 schema:value 10.1038/nbt0196-50
89 rdf:type schema:PropertyValue
90 N361ac95fcaaf41f2bf7776e95938cda4 schema:name pubmed_id
91 schema:value 9636312
92 rdf:type schema:PropertyValue
93 N46d89a46d9f94ed9aaa08cea43984bf5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Amino Acyl-tRNA Synthetases
95 rdf:type schema:DefinedTerm
96 N4e0349809d214f71bd99c51f7e383f44 schema:issueNumber 1
97 rdf:type schema:PublicationIssue
98 N4e695d27eaef40bba3959be9f7f99fce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Antibodies, Monoclonal
100 rdf:type schema:DefinedTerm
101 N561f2c9e4a8c4591a4a4e46fc6f60d8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Artificial Gene Fusion
103 rdf:type schema:DefinedTerm
104 N678a7caebdf54638840eb6462b7942c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Mutation
106 rdf:type schema:DefinedTerm
107 N69fd0afd29e945ebb0359eca1e268986 schema:name Springer Nature - SN SciGraph project
108 rdf:type schema:Organization
109 N8d6894ae4f3d4e8cb632cab6841f2a75 schema:volumeNumber 14
110 rdf:type schema:PublicationVolume
111 N8e4a649196cc48e58a5647bcc404bcf9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Chromosome Deletion
113 rdf:type schema:DefinedTerm
114 N93c2b1cdecaa451c864969b07aea8773 schema:name nlm_unique_id
115 schema:value 8309273
116 rdf:type schema:PropertyValue
117 Na9a705b49fbd40ca86e4b51ddfdcaaea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Antibody Specificity
119 rdf:type schema:DefinedTerm
120 Nac8f07c459b649478248ee8c92bb0174 rdf:first sg:person.01371027336.47
121 rdf:rest Nf3646b01accb4650a2616dee2b23bdca
122 Nb19e20f58d154a2fbed3a54773624d9c rdf:first sg:person.01270013225.04
123 rdf:rest Nac8f07c459b649478248ee8c92bb0174
124 Ned13928637214afd8a847982612a2cb3 rdf:first sg:person.01276144100.08
125 rdf:rest Nb19e20f58d154a2fbed3a54773624d9c
126 Nf3646b01accb4650a2616dee2b23bdca rdf:first sg:person.012067245622.45
127 rdf:rest rdf:nil
128 Nfc6d166814904a9fa95145b2ecc5f3b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Genes, Bacterial
130 rdf:type schema:DefinedTerm
131 Nfe84ba5a238b495794ed317ef2d33845 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Plasmids
133 rdf:type schema:DefinedTerm
134 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
135 schema:name Biological Sciences
136 rdf:type schema:DefinedTerm
137 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
138 schema:name Biochemistry and Cell Biology
139 rdf:type schema:DefinedTerm
140 sg:journal.1094195 schema:issn 0733-222X
141 schema:name Bio/Technology
142 rdf:type schema:Periodical
143 sg:person.012067245622.45 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
144 schema:familyName Söll
145 schema:givenName Dieter
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012067245622.45
147 rdf:type schema:Person
148 sg:person.01270013225.04 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
149 schema:familyName Ibba
150 schema:givenName Michael
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270013225.04
152 rdf:type schema:Person
153 sg:person.01276144100.08 schema:affiliation N27fe0109681441a4862cbed5864ac9f4
154 schema:familyName Thomann
155 schema:givenName Hans-Ulrich
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276144100.08
157 rdf:type schema:Person
158 sg:person.01371027336.47 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
159 schema:familyName Hong
160 schema:givenName Kwang-Won
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371027336.47
162 rdf:type schema:Person
163 sg:pub.10.1007/bf00331173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001762846
164 https://doi.org/10.1007/bf00331173
165 rdf:type schema:CreativeWork
166 sg:pub.10.1038/347203a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036757994
167 https://doi.org/10.1038/347203a0
168 rdf:type schema:CreativeWork
169 sg:pub.10.1038/352213a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052831152
170 https://doi.org/10.1038/352213a0
171 rdf:type schema:CreativeWork
172 sg:pub.10.1038/nbt1088-1204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001334427
173 https://doi.org/10.1038/nbt1088-1204
174 rdf:type schema:CreativeWork
175 https://app.dimensions.ai/details/publication/pub.1079492588 schema:CreativeWork
176 https://app.dimensions.ai/details/publication/pub.1081662991 schema:CreativeWork
177 https://doi.org/10.1002/j.1460-2075.1993.tb06215.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082390200
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/0022-0248(88)90312-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018166970
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/0022-2836(91)90740-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1026149744
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/0076-6879(90)85004-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035004964
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/0092-8674(84)90412-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048251785
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/0092-8674(87)90086-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042213289
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/0378-1119(88)90558-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022539367
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/0378-1119(89)90257-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051883881
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/s0076-6879(85)13011-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001552528
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/s0968-0004(00)89045-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034825303
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1021/bi00004a001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055156739
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1021/bi00015a038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055157286
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1021/bi00239a009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055166600
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1073/pnas.80.16.4949 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039287430
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1073/pnas.82.17.5681 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039128689
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1073/pnas.82.2.488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026991187
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1073/pnas.86.3.968 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050973501
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1080/15257779408012173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014022332
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1093/protein/2.4.293 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059980419
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1111/j.1365-2958.1989.tb00197.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043421435
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1126/science.7701322 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062648974
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1128/jb.173.8.2633-2638.1991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062720352
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1128/mcb.8.5.2159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003447716
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1128/mcb.9.12.5387 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039878546
224 rdf:type schema:CreativeWork
225 https://www.grid.ac/institutes/grid.47100.32 schema:alternateName Yale University
226 schema:name Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
227 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...