Molecular Cloning and Regulation of a Mineral Phosphate Solubilizing Gene from Erwinia Herbicola View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-01-01

AUTHORS

A. H. Goldstein, S. T. Liu

ABSTRACT

At levels less than 1mM of soluble orthophosphate (Pi) E. herbicola solubilizes dicalcium phosphate (DCP) or hydroxyapatite (HAP) in the external culture media. E. coli is capable of solubilizing dicalcium phosphate but not hydroxyapatite. The mineral phosphate solubilizing (MPS) trait is induced (repressed) by low (high) levels of exogenous Pi. A cosmid library of E. herbicola genomic DNA was constructed in E. coli. Screening for the MPS trait resulted in the isolation of a recombinant clone that showed inducible/repressible hydroxapatite solubilization at a level comparable with E. herbicola. We propose that these genes play a role in bacterial phosphate starvation metabolism. More... »

PAGES

72-74

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt0187-72

DOI

http://dx.doi.org/10.1038/nbt0187-72

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011547576


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Plant Sciences, University Of Arizona, 85721, Tucson, AZ", 
          "id": "http://www.grid.ac/institutes/grid.134563.6", 
          "name": [
            "Department of Plant Sciences, University Of Arizona, 85721, Tucson, AZ"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Goldstein", 
        "givenName": "A. H.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Plant Sciences, University Of Arizona, 85721, Tucson, AZ", 
          "id": "http://www.grid.ac/institutes/grid.134563.6", 
          "name": [
            "Department of Plant Sciences, University Of Arizona, 85721, Tucson, AZ"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "S. T.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/289085a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010175313", 
          "https://doi.org/10.1038/289085a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/180994a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004459457", 
          "https://doi.org/10.1038/180994a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1987-01-01", 
    "datePublishedReg": "1987-01-01", 
    "description": "At levels less than 1mM of soluble orthophosphate (Pi) E. herbicola solubilizes dicalcium phosphate (DCP) or hydroxyapatite (HAP) in the external culture media. E. coli is capable of solubilizing dicalcium phosphate but not hydroxyapatite. The mineral phosphate solubilizing (MPS) trait is induced (repressed) by low (high) levels of exogenous Pi. A cosmid library of E. herbicola genomic DNA was constructed in E. coli. Screening for the MPS trait resulted in the isolation of a recombinant clone that showed inducible/repressible hydroxapatite solubilization at a level comparable with E. herbicola. We propose that these genes play a role in bacterial phosphate starvation metabolism.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/nbt0187-72", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1115214", 
        "issn": [
          "1087-0156", 
          "1546-1696"
        ], 
        "name": "Nature Biotechnology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "keywords": [
      "mineral phosphate", 
      "E. herbicola", 
      "E. coli", 
      "Molecular cloning", 
      "cosmid library", 
      "external culture medium", 
      "genomic DNA", 
      "recombinant clones", 
      "Erwinia herbicola", 
      "herbicola", 
      "exogenous Pi", 
      "genes", 
      "traits", 
      "coli", 
      "culture medium", 
      "cloning", 
      "DNA", 
      "clones", 
      "regulation", 
      "low levels", 
      "phosphate", 
      "metabolism", 
      "solubilization", 
      "starvation metabolism", 
      "isolation", 
      "levels", 
      "library", 
      "PI", 
      "role", 
      "medium", 
      "dicalcium phosphate", 
      "dicalcium"
    ], 
    "name": "Molecular Cloning and Regulation of a Mineral Phosphate Solubilizing Gene from Erwinia Herbicola", 
    "pagination": "72-74", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011547576"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nbt0187-72"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nbt0187-72", 
      "https://app.dimensions.ai/details/publication/pub.1011547576"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_182.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/nbt0187-72"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt0187-72'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt0187-72'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt0187-72'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt0187-72'


 

This table displays all metadata directly associated to this object as RDF triples.

102 TRIPLES      21 PREDICATES      58 URIs      48 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nbt0187-72 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author Nf110012b3f124ae2bf2d1b3fe975ac51
4 schema:citation sg:pub.10.1038/180994a0
5 sg:pub.10.1038/289085a0
6 schema:datePublished 1987-01-01
7 schema:datePublishedReg 1987-01-01
8 schema:description At levels less than 1mM of soluble orthophosphate (Pi) E. herbicola solubilizes dicalcium phosphate (DCP) or hydroxyapatite (HAP) in the external culture media. E. coli is capable of solubilizing dicalcium phosphate but not hydroxyapatite. The mineral phosphate solubilizing (MPS) trait is induced (repressed) by low (high) levels of exogenous Pi. A cosmid library of E. herbicola genomic DNA was constructed in E. coli. Screening for the MPS trait resulted in the isolation of a recombinant clone that showed inducible/repressible hydroxapatite solubilization at a level comparable with E. herbicola. We propose that these genes play a role in bacterial phosphate starvation metabolism.
9 schema:genre article
10 schema:isAccessibleForFree false
11 schema:isPartOf N387982d9128c42eca91730de20b9928e
12 N43b2451ed67f4c5b94f1c82b39e6cbcc
13 sg:journal.1115214
14 schema:keywords DNA
15 E. coli
16 E. herbicola
17 Erwinia herbicola
18 Molecular cloning
19 PI
20 clones
21 cloning
22 coli
23 cosmid library
24 culture medium
25 dicalcium
26 dicalcium phosphate
27 exogenous Pi
28 external culture medium
29 genes
30 genomic DNA
31 herbicola
32 isolation
33 levels
34 library
35 low levels
36 medium
37 metabolism
38 mineral phosphate
39 phosphate
40 recombinant clones
41 regulation
42 role
43 solubilization
44 starvation metabolism
45 traits
46 schema:name Molecular Cloning and Regulation of a Mineral Phosphate Solubilizing Gene from Erwinia Herbicola
47 schema:pagination 72-74
48 schema:productId Nbe14d14d574d48c2b5d17b84a32686a9
49 Neb67e27508744727b2246f131870e0bc
50 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011547576
51 https://doi.org/10.1038/nbt0187-72
52 schema:sdDatePublished 2022-12-01T06:18
53 schema:sdLicense https://scigraph.springernature.com/explorer/license/
54 schema:sdPublisher Nb1d4a7b6828043b99935d2c2a1c1193d
55 schema:url https://doi.org/10.1038/nbt0187-72
56 sgo:license sg:explorer/license/
57 sgo:sdDataset articles
58 rdf:type schema:ScholarlyArticle
59 N1687f6574abc45ad851fe30d44f6e5a5 schema:affiliation grid-institutes:grid.134563.6
60 schema:familyName Goldstein
61 schema:givenName A. H.
62 rdf:type schema:Person
63 N387982d9128c42eca91730de20b9928e schema:volumeNumber 5
64 rdf:type schema:PublicationVolume
65 N43b2451ed67f4c5b94f1c82b39e6cbcc schema:issueNumber 1
66 rdf:type schema:PublicationIssue
67 N84f47b732b1f4af6bdc12287a4d2149a rdf:first N9d189515a4f144bb9d71f4b8f7e1f089
68 rdf:rest rdf:nil
69 N9d189515a4f144bb9d71f4b8f7e1f089 schema:affiliation grid-institutes:grid.134563.6
70 schema:familyName Liu
71 schema:givenName S. T.
72 rdf:type schema:Person
73 Nb1d4a7b6828043b99935d2c2a1c1193d schema:name Springer Nature - SN SciGraph project
74 rdf:type schema:Organization
75 Nbe14d14d574d48c2b5d17b84a32686a9 schema:name dimensions_id
76 schema:value pub.1011547576
77 rdf:type schema:PropertyValue
78 Neb67e27508744727b2246f131870e0bc schema:name doi
79 schema:value 10.1038/nbt0187-72
80 rdf:type schema:PropertyValue
81 Nf110012b3f124ae2bf2d1b3fe975ac51 rdf:first N1687f6574abc45ad851fe30d44f6e5a5
82 rdf:rest N84f47b732b1f4af6bdc12287a4d2149a
83 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
84 schema:name Biological Sciences
85 rdf:type schema:DefinedTerm
86 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
87 schema:name Genetics
88 rdf:type schema:DefinedTerm
89 sg:journal.1115214 schema:issn 1087-0156
90 1546-1696
91 schema:name Nature Biotechnology
92 schema:publisher Springer Nature
93 rdf:type schema:Periodical
94 sg:pub.10.1038/180994a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004459457
95 https://doi.org/10.1038/180994a0
96 rdf:type schema:CreativeWork
97 sg:pub.10.1038/289085a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010175313
98 https://doi.org/10.1038/289085a0
99 rdf:type schema:CreativeWork
100 grid-institutes:grid.134563.6 schema:alternateName Department of Plant Sciences, University Of Arizona, 85721, Tucson, AZ
101 schema:name Department of Plant Sciences, University Of Arizona, 85721, Tucson, AZ
102 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...