Ontology type: schema:ScholarlyArticle
2018-05-07
AUTHORSKevin R Roy, Justin D Smith, Sibylle C Vonesch, Gen Lin, Chelsea Szu Tu, Alex R Lederer, Angela Chu, Sundari Suresh, Michelle Nguyen, Joe Horecka, Ashutosh Tripathi, Wallace T Burnett, Maddison A Morgan, Julia Schulz, Kevin M Orsley, Wu Wei, Raeka S Aiyar, Ronald W Davis, Vytas A Bankaitis, James E Haber, Marc L Salit, Robert P St Onge, Lars M Steinmetz
ABSTRACTOur understanding of how genotype controls phenotype is limited by the scale at which we can precisely alter the genome and assess the phenotypic consequences of each perturbation. Here we describe a CRISPR-Cas9-based method for multiplexed accurate genome editing with short, trackable, integrated cellular barcodes (MAGESTIC) in Saccharomyces cerevisiae. MAGESTIC uses array-synthesized guide-donor oligos for plasmid-based high-throughput editing and features genomic barcode integration to prevent plasmid barcode loss and to enable robust phenotyping. We demonstrate that editing efficiency can be increased more than fivefold by recruiting donor DNA to the site of breaks using the LexA-Fkh1p fusion protein. We performed saturation editing of the essential gene SEC14 and identified amino acids critical for chemical inhibition of lipid signaling. We also constructed thousands of natural genetic variants, characterized guide mismatch tolerance at the genome scale, and ascertained that cryptic Pol III termination elements substantially reduce guide efficacy. MAGESTIC will be broadly useful to uncover the genetic basis of phenotypes in yeast. More... »
PAGES512
http://scigraph.springernature.com/pub.10.1038/nbt.4137
DOIhttp://dx.doi.org/10.1038/nbt.4137
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1103843570
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/29734294
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Genome-Scale Measurements Group, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.",
"Joint Initiative for Metrology in Biology, Stanford, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Roy",
"givenName": "Kevin R",
"id": "sg:person.01051533370.39",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051533370.39"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Smith",
"givenName": "Justin D",
"id": "sg:person.01254201353.26",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254201353.26"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "European Molecular Biology Laboratory",
"id": "https://www.grid.ac/institutes/grid.4709.a",
"name": [
"European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Heidelberg, Germany."
],
"type": "Organization"
},
"familyName": "Vonesch",
"givenName": "Sibylle C",
"id": "sg:person.01240551342.74",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240551342.74"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "European Molecular Biology Laboratory",
"id": "https://www.grid.ac/institutes/grid.4709.a",
"name": [
"European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Heidelberg, Germany."
],
"type": "Organization"
},
"familyName": "Lin",
"givenName": "Gen",
"id": "sg:person.01036305526.43",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036305526.43"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "European Molecular Biology Laboratory",
"id": "https://www.grid.ac/institutes/grid.4709.a",
"name": [
"European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Heidelberg, Germany."
],
"type": "Organization"
},
"familyName": "Tu",
"givenName": "Chelsea Szu",
"id": "sg:person.015605261451.33",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015605261451.33"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "European Molecular Biology Laboratory",
"id": "https://www.grid.ac/institutes/grid.4709.a",
"name": [
"European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Heidelberg, Germany."
],
"type": "Organization"
},
"familyName": "Lederer",
"givenName": "Alex R",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Chu",
"givenName": "Angela",
"id": "sg:person.01114415020.70",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114415020.70"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Suresh",
"givenName": "Sundari",
"id": "sg:person.01270503673.43",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270503673.43"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Nguyen",
"givenName": "Michelle",
"id": "sg:person.0760416166.73",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760416166.73"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Horecka",
"givenName": "Joe",
"id": "sg:person.01103521154.64",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103521154.64"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Texas A&M Health Science Center",
"id": "https://www.grid.ac/institutes/grid.412408.b",
"name": [
"Department of Molecular and Cellular Medicine, Texas A&M Health Science Center, College Station, Texas, USA."
],
"type": "Organization"
},
"familyName": "Tripathi",
"givenName": "Ashutosh",
"id": "sg:person.0645212510.27",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645212510.27"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Burnett",
"givenName": "Wallace T",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Morgan",
"givenName": "Maddison A",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Schulz",
"givenName": "Julia",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Orsley",
"givenName": "Kevin M",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Wei",
"givenName": "Wu",
"id": "sg:person.01251154326.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251154326.08"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Lucile Packard Children's Hospital",
"id": "https://www.grid.ac/institutes/grid.414123.1",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA."
],
"type": "Organization"
},
"familyName": "Aiyar",
"givenName": "Raeka S",
"id": "sg:person.01244207155.14",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244207155.14"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.",
"Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Davis",
"givenName": "Ronald W",
"id": "sg:person.015333701302.31",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015333701302.31"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Texas A&M University",
"id": "https://www.grid.ac/institutes/grid.264756.4",
"name": [
"Department of Molecular and Cellular Medicine, Texas A&M Health Science Center, College Station, Texas, USA.",
"Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.",
"Department of Chemistry, Texas A&M University, College Station, Texas, USA."
],
"type": "Organization"
},
"familyName": "Bankaitis",
"givenName": "Vytas A",
"id": "sg:person.0707047150.40",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707047150.40"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Brandeis University",
"id": "https://www.grid.ac/institutes/grid.253264.4",
"name": [
"Rosenstiel Basic Medical Sciences Research Center and Department of Biology, Brandeis University, Waltham, Massachusetts, USA."
],
"type": "Organization"
},
"familyName": "Haber",
"givenName": "James E",
"id": "sg:person.0756636704.81",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756636704.81"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Institute of Standards and Technology",
"id": "https://www.grid.ac/institutes/grid.94225.38",
"name": [
"Genome-Scale Measurements Group, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.",
"Joint Initiative for Metrology in Biology, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "Salit",
"givenName": "Marc L",
"id": "sg:person.0635305134.21",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635305134.21"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Stanford University",
"id": "https://www.grid.ac/institutes/grid.168010.e",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA."
],
"type": "Organization"
},
"familyName": "St Onge",
"givenName": "Robert P",
"id": "sg:person.01156724075.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156724075.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "European Molecular Biology Laboratory",
"id": "https://www.grid.ac/institutes/grid.4709.a",
"name": [
"Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA.",
"Joint Initiative for Metrology in Biology, Stanford, California, USA.",
"Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.",
"European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Heidelberg, Germany."
],
"type": "Organization"
},
"familyName": "Steinmetz",
"givenName": "Lars M",
"id": "sg:person.0717243773.89",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717243773.89"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s002940050160",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000832483",
"https://doi.org/10.1007/s002940050160"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s002940050160",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000832483",
"https://doi.org/10.1007/s002940050160"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkw417",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002921897"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pgen.1002630",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009798514"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature16526",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010800066",
"https://doi.org/10.1038/nature16526"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.2647",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012543463",
"https://doi.org/10.1038/nbt.2647"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-2836(05)80360-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013618994"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.3437",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014083513",
"https://doi.org/10.1038/nbt.3437"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35179",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015562929",
"https://doi.org/10.1038/35179"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35179",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015562929",
"https://doi.org/10.1038/35179"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m412238200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016675724"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(91)90508-v",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019622190"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.aad5227",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020934192"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.2021311",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020992243"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1083/jcb.200201037",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021400308"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/bioinformatics/btp616",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023247882"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nprot.2007.13",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025098576",
"https://doi.org/10.1038/nprot.2007.13"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.1389",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025958911",
"https://doi.org/10.1038/nchembio.1389"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkr074",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028778018"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0092-8674(00)81345-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029019737"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/jmbi.1996.0897",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031514441"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2958.2011.07632.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032367639"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.3026",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033528408",
"https://doi.org/10.1038/nbt.3026"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.7554/elife.03703",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039365894"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.3718",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040330800",
"https://doi.org/10.1038/nbt.3718"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkt135",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042813151"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.994902",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045361220"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gks042",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045678648"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pgen.1006094",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052899813"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pgen.1006094",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052899813"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bbagrm.2012.10.006",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053429324"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1109/mcse.2007.55",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1061398159"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074750629",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1996.tb01036.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083023825"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.15252/msb.20167233",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083814806"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cr.2017.29",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1084127932",
"https://doi.org/10.1038/cr.2017.29"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cr.2017.29",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1084127932",
"https://doi.org/10.1038/cr.2017.29"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature24268",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091860712",
"https://doi.org/10.1038/nature24268"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature24268",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091860712",
"https://doi.org/10.1038/nature24268"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/147637",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091919612"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/147637",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091919612"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/147637",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091919612"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/195776",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092003119"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/195776",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092003119"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/195776",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092003119"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.4466",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092234892",
"https://doi.org/10.1038/nmeth.4466"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.4466",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092234892",
"https://doi.org/10.1038/nmeth.4466"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/204339",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092272208"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/204339",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092272208"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/204339",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092272208"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/218149",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092688285"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/218149",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092688285"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/218149",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092688285"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature26155",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101264077",
"https://doi.org/10.1038/nature26155"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature26155",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101264077",
"https://doi.org/10.1038/nature26155"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.4147",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104126101",
"https://doi.org/10.1038/nbt.4147"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.4147",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104126101",
"https://doi.org/10.1038/nbt.4147"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.7554/elife.33761",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104271286"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.7554/elife.33761",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104271286"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.7554/elife.33761",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104271286"
],
"type": "CreativeWork"
}
],
"datePublished": "2018-05-07",
"datePublishedReg": "2018-05-07",
"description": "Our understanding of how genotype controls phenotype is limited by the scale at which we can precisely alter the genome and assess the phenotypic consequences of each perturbation. Here we describe a CRISPR-Cas9-based method for multiplexed accurate genome editing with short, trackable, integrated cellular barcodes (MAGESTIC) in Saccharomyces cerevisiae. MAGESTIC uses array-synthesized guide-donor oligos for plasmid-based high-throughput editing and features genomic barcode integration to prevent plasmid barcode loss and to enable robust phenotyping. We demonstrate that editing efficiency can be increased more than fivefold by recruiting donor DNA to the site of breaks using the LexA-Fkh1p fusion protein. We performed saturation editing of the essential gene SEC14 and identified amino acids critical for chemical inhibition of lipid signaling. We also constructed thousands of natural genetic variants, characterized guide mismatch tolerance at the genome scale, and ascertained that cryptic Pol III termination elements substantially reduce guide efficacy. MAGESTIC will be broadly useful to uncover the genetic basis of phenotypes in yeast.",
"genre": "research_article",
"id": "sg:pub.10.1038/nbt.4137",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isFundedItemOf": [
{
"id": "sg:grant.2635010",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.3792509",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.3623681",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2516705",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2513255",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2436656",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.5238597",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.7072759",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1115214",
"issn": [
"1087-0156",
"1546-1696"
],
"name": "Nature Biotechnology",
"type": "Periodical"
},
{
"issueNumber": "6",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "36"
}
],
"name": "Multiplexed precision genome editing with trackable genomic barcodes in yeast",
"pagination": "512",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"d8593b5cf7cd8f6b0c1c30fb08ac3309499fc932d57e1a1da604bd5609cda992"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"29734294"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9604648"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/nbt.4137"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1103843570"
]
}
],
"sameAs": [
"https://doi.org/10.1038/nbt.4137",
"https://app.dimensions.ai/details/publication/pub.1103843570"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T01:23",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000609.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/nbt.4137"
}
]Download the RDF metadata as:Â json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt.4137'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt.4137'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt.4137'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt.4137'
This table displays all metadata directly associated to this object as RDF triples.
401 TRIPLES
21 PREDICATES
70 URIs
20 LITERALS
9 BLANK NODES