Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-03-27

AUTHORS

Zenpei Shimatani, Sachiko Kashojiya, Mariko Takayama, Rie Terada, Takayuki Arazoe, Hisaki Ishii, Hiroshi Teramura, Tsuyoshi Yamamoto, Hiroki Komatsu, Kenji Miura, Hiroshi Ezura, Keiji Nishida, Tohru Ariizumi, Akihiko Kondo

ABSTRACT

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement. More... »

PAGES

441-443

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt.3833

DOI

http://dx.doi.org/10.1038/nbt.3833

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084128666

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28346401


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