Ontology type: schema:ScholarlyArticle
2016-06
AUTHORSBurkhard Steuernagel, Sambasivam K Periyannan, Inmaculada Hernández-Pinzón, Kamil Witek, Matthew N Rouse, Guotai Yu, Asyraf Hatta, Mick Ayliffe, Harbans Bariana, Jonathan D G Jones, Evans S Lagudah, Brande B H Wulff
ABSTRACTWild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5-15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution. If several cloned R genes were available, it would be possible to pyramid R genes in a crop, which might provide more durable resistance. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize. More... »
PAGES652-655
http://scigraph.springernature.com/pub.10.1038/nbt.3543
DOIhttp://dx.doi.org/10.1038/nbt.3543
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1008149752
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/27111722
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"sameAs": [
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"sdDataset": "articles",
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]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt.3543'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt.3543'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt.3543'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt.3543'
This table displays all metadata directly associated to this object as RDF triples.
349 TRIPLES
21 PREDICATES
78 URIs
30 LITERALS
18 BLANK NODES