Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-05

AUTHORS

Hanhui Ma, Li-Chun Tu, Ardalan Naseri, Maximiliaan Huisman, Shaojie Zhang, David Grunwald, Thoru Pederson

ABSTRACT

A lack of techniques to image multiple genomic loci in living cells has limited our ability to investigate chromosome dynamics. Here we describe CRISPRainbow, a system for labeling DNA in living cells based on nuclease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bind sets of fluorescent proteins. We demonstrate simultaneous imaging of up to six chromosomal loci in individual live cells and document large differences in the dynamic properties of different chromosomal loci. More... »

PAGES

528-530

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt.3526

DOI

http://dx.doi.org/10.1038/nbt.3526

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007681398

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27088723


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