Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-01-18

AUTHORS

John G Doench, Nicolo Fusi, Meagan Sullender, Mudra Hegde, Emma W Vaimberg, Katherine F Donovan, Ian Smith, Zuzana Tothova, Craig Wilen, Robert Orchard, Herbert W Virgin, Jennifer Listgarten, David E Root

ABSTRACT

Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and off-target prediction algorithms.

PAGES

184-191

References to SciGraph publications

  • 2014-01-29. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9 in NATURE
  • 2014-01-05. Discovery and saturation analysis of cancer genes across 21 tumour types in NATURE
  • 2013-12-23. Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library in NATURE BIOTECHNOLOGY
  • 2010-11-24. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation in NATURE
  • 2013-07-21. DNA targeting specificity of RNA-guided Cas9 nucleases in NATURE BIOTECHNOLOGY
  • 2014-12-05. MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens in GENOME BIOLOGY
  • 2013-06-23. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells in NATURE BIOTECHNOLOGY
  • 2010-09-07. Clinical efficacy of a RAF inhibitor needs broad target blockade in BRAF-mutant melanoma in NATURE
  • 2014-12-16. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases in NATURE BIOTECHNOLOGY
  • 2014-01-30. E-CRISP: fast CRISPR target site identification in NATURE METHODS
  • 2012-05-31. A comparative review of cell culture systems for the study of microglial biology in Alzheimer’s disease in JOURNAL OF NEUROINFLAMMATION
  • 2014-09-03. Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation in NATURE BIOTECHNOLOGY
  • 2015-07-13. Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach in NATURE METHODS
  • 2015-05-11. Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains in NATURE BIOTECHNOLOGY
  • 2014-06-27. Microhomology-based choice of Cas9 nuclease target sites in NATURE METHODS
  • 2012-03-28. The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity in NATURE
  • 2006-02. Acute lymphoblastic leukaemia: a model for the pharmacogenomics of cancer therapy in NATURE REVIEWS CANCER
  • 2014-04-25. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification in NATURE BIOTECHNOLOGY
  • 2014-07-30. Improved vectors and genome-wide libraries for CRISPR screening in NATURE METHODS
  • 2012-03-04. Fast gapped-read alignment with Bowtie 2 in NATURE METHODS
  • Journal

    TITLE

    Nature Biotechnology

    ISSUE

    2

    VOLUME

    34

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.3437

    DOI

    http://dx.doi.org/10.1038/nbt.3437

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014083513

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26780180


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