DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-11

AUTHORS

Je Wook Woo, Jungeun Kim, Soon Il Kwon, Claudia Corvalán, Seung Woo Cho, Hyeran Kim, Sang-Gyu Kim, Sang-Tae Kim, Sunghwa Choe, Jin-Soo Kim

ABSTRACT

Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation. More... »

PAGES

1162-1164

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt.3389

DOI

http://dx.doi.org/10.1038/nbt.3389

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028917872

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26479191


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