Photoactivatable CRISPR-Cas9 for optogenetic genome editing View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-07

AUTHORS

Yuta Nihongaki, Fuun Kawano, Takahiro Nakajima, Moritoshi Sato

ABSTRACT

We describe an engineered photoactivatable Cas9 (paCas9) that enables optogenetic control of CRISPR-Cas9 genome editing in human cells. paCas9 consists of split Cas9 fragments and photoinducible dimerization domains named Magnets. In response to blue light irradiation, paCas9 expressed in human embryonic kidney 293T cells induces targeted genome sequence modifications through both nonhomologous end joining and homology-directed repair pathways. Genome editing activity can be switched off simply by extinguishing the light. We also demonstrate activation of paCas9 in spatial patterns determined by the sites of irradiation. Optogenetic control of targeted genome editing should facilitate improved understanding of complex gene networks and could prove useful in biomedical applications. More... »

PAGES

755-760

References to SciGraph publications

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  • 2014-09. Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease in NATURE
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  • 2013-03. Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.3245

    DOI

    http://dx.doi.org/10.1038/nbt.3245

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011569935

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26076431


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