Conditional targeted genome editing using somatically expressed TALENs in C. elegans View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-10

AUTHORS

Ze Cheng, Peishan Yi, Xiangming Wang, Yongping Chai, Guoxin Feng, Yihong Yang, Xing Liang, Zhiwen Zhu, Wei Li, Guangshuo Ou

ABSTRACT

We have developed a method for the generation of conditional knockouts in Caenorhabditis elegans by expressing transcription activator-like effector nucleases (TALENs) in somatic cells. Using germline transformation with plasmids encoding TALENs under the control of an inducible or tissue-specific promoter, we observed effective gene modifications and resulting phenotypes in specific developmental stages and tissues. We further used this method to bypass the embryonic requirement of cor-1, which encodes the homolog of human severe combined immunodeficiency (SCID) protein coronin, and we determined its essential role in cell migration in larval Q-cell lineages. Our results show that TALENs expressed in the somatic cells of model organisms provide a versatile tool for functional genomics. More... »

PAGES

934-937

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt.2674

DOI

http://dx.doi.org/10.1038/nbt.2674

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006594155

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23955274


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caenorhabditis elegans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Lineage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Movement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Deoxyribonucleases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genome, Helminth", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luminescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mechanotransduction, Cellular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA Editing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trans-Activators", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Peking University", 
          "id": "https://www.grid.ac/institutes/grid.11135.37", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China.", 
            "School of Life Sciences, Peking University, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cheng", 
        "givenName": "Ze", 
        "id": "sg:person.0777356210.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777356210.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yi", 
        "givenName": "Peishan", 
        "id": "sg:person.01230724076.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230724076.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Xiangming", 
        "id": "sg:person.01306337250.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306337250.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chai", 
        "givenName": "Yongping", 
        "id": "sg:person.01345152476.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345152476.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Feng", 
        "givenName": "Guoxin", 
        "id": "sg:person.01061523350.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061523350.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Yihong", 
        "id": "sg:person.01013410150.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013410150.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liang", 
        "givenName": "Xing", 
        "id": "sg:person.012610261547.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012610261547.89"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhu", 
        "givenName": "Zhiwen", 
        "id": "sg:person.0667661576.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667661576.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Wei", 
        "id": "sg:person.016205376120.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016205376120.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biophysics", 
          "id": "https://www.grid.ac/institutes/grid.418856.6", 
          "name": [
            "National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ou", 
        "givenName": "Guangshuo", 
        "id": "sg:person.01025562770.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025562770.75"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nmeth.1539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010147854", 
          "https://doi.org/10.1038/nmeth.1539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010147854", 
          "https://doi.org/10.1038/nmeth.1539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1939", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010610351", 
          "https://doi.org/10.1038/nbt.1939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1755", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016004947", 
          "https://doi.org/10.1038/nbt.1755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.3.2.221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017206456"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2006.11.065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022245162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(86)90668-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022604578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1454", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023315310", 
          "https://doi.org/10.1038/nmeth.1454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-006-6012-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027216176", 
          "https://doi.org/10.1007/s00018-006-6012-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni.1662", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028044442", 
          "https://doi.org/10.1038/ni.1662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/g3.112.003830", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028372231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/g3.112.003830", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028372231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2012.11.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030194691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1463", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032555226", 
          "https://doi.org/10.1038/nmeth.1463"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1463", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032555226", 
          "https://doi.org/10.1038/nmeth.1463"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1130563", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035876594"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.1003352", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038120373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth0710-488a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038282017", 
          "https://doi.org/10.1038/nmeth0710-488a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth0710-488a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038282017", 
          "https://doi.org/10.1038/nmeth0710-488a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2012.128", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038902182", 
          "https://doi.org/10.1038/nprot.2012.128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tcb.2011.04.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041640330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1226201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048506578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1000028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049457203"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm3486", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050461034", 
          "https://doi.org/10.1038/nrm3486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0016644", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051334047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1207773", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062464819"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1991.tb04966.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077884622"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-10", 
    "datePublishedReg": "2013-10-01", 
    "description": "We have developed a method for the generation of conditional knockouts in Caenorhabditis elegans by expressing transcription activator-like effector nucleases (TALENs) in somatic cells. Using germline transformation with plasmids encoding TALENs under the control of an inducible or tissue-specific promoter, we observed effective gene modifications and resulting phenotypes in specific developmental stages and tissues. We further used this method to bypass the embryonic requirement of cor-1, which encodes the homolog of human severe combined immunodeficiency (SCID) protein coronin, and we determined its essential role in cell migration in larval Q-cell lineages. Our results show that TALENs expressed in the somatic cells of model organisms provide a versatile tool for functional genomics. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nbt.2674", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.7006882", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.7019462", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.7011788", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.7183558", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6992538", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.7006596", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1115214", 
        "issn": [
          "1087-0156", 
          "1546-1696"
        ], 
        "name": "Nature Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "31"
      }
    ], 
    "name": "Conditional targeted genome editing using somatically expressed TALENs in C. elegans", 
    "pagination": "934-937", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1f687b1707bc3cb98e856a8059041b48583952f2b1338251a9bf27a2d63b1596"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23955274"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9604648"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nbt.2674"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1006594155"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nbt.2674", 
      "https://app.dimensions.ai/details/publication/pub.1006594155"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:46", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000421.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/nbt.2674"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt.2674'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt.2674'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt.2674'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt.2674'


 

This table displays all metadata directly associated to this object as RDF triples.

291 TRIPLES      21 PREDICATES      68 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nbt.2674 schema:about N0071020127fc498fa218b348cc266fab
2 N007e0a6c98164edb9eb66377c666ca7b
3 N2ca0d9eebb1e443ba237e5c5c6103e02
4 N3a5ba8d6537444ebaa51fda5593e5a60
5 N46dbdcf9c5c44e7e9ba36c63f6af55ee
6 N49946105f6d9472a815f0ef78f79b7c5
7 N5bbaec444bab4a36a5b95e07f4430b1f
8 N5f67b860c5e7411a8174b6a8804ae6a3
9 N733765684a4046ac9d1179462b9a1351
10 N8266b5b97dcf478f9480dce4b66520ae
11 Nb753986b38504add82b61edbaa12ac8e
12 Nc0689bc32c4a43ac9abac26fe13ee85c
13 Nda27910f354b4c7d85b56b813de62032
14 Nef47ebe8bf0a478eb05a118d7bc080af
15 Nf741a64afc1547afa8d86549a9faec15
16 Nf79c0f8c318841868b8f90c0229ed72c
17 anzsrc-for:06
18 anzsrc-for:0604
19 schema:author N994d35ce47bd44caa972fb33fe160216
20 schema:citation sg:pub.10.1007/s00018-006-6012-z
21 sg:pub.10.1038/nbt.1755
22 sg:pub.10.1038/nbt.1939
23 sg:pub.10.1038/ni.1662
24 sg:pub.10.1038/nmeth.1454
25 sg:pub.10.1038/nmeth.1463
26 sg:pub.10.1038/nmeth.1539
27 sg:pub.10.1038/nmeth0710-488a
28 sg:pub.10.1038/nprot.2012.128
29 sg:pub.10.1038/nrm3486
30 https://doi.org/10.1002/j.1460-2075.1991.tb04966.x
31 https://doi.org/10.1016/0092-8674(86)90668-9
32 https://doi.org/10.1016/j.cub.2006.11.065
33 https://doi.org/10.1016/j.stem.2012.11.011
34 https://doi.org/10.1016/j.tcb.2011.04.004
35 https://doi.org/10.1091/mbc.3.2.221
36 https://doi.org/10.1126/science.1130563
37 https://doi.org/10.1126/science.1207773
38 https://doi.org/10.1126/science.1226201
39 https://doi.org/10.1371/journal.pgen.1000028
40 https://doi.org/10.1371/journal.pone.0016644
41 https://doi.org/10.1534/g3.112.003830
42 https://doi.org/10.4049/jimmunol.1003352
43 schema:datePublished 2013-10
44 schema:datePublishedReg 2013-10-01
45 schema:description We have developed a method for the generation of conditional knockouts in Caenorhabditis elegans by expressing transcription activator-like effector nucleases (TALENs) in somatic cells. Using germline transformation with plasmids encoding TALENs under the control of an inducible or tissue-specific promoter, we observed effective gene modifications and resulting phenotypes in specific developmental stages and tissues. We further used this method to bypass the embryonic requirement of cor-1, which encodes the homolog of human severe combined immunodeficiency (SCID) protein coronin, and we determined its essential role in cell migration in larval Q-cell lineages. Our results show that TALENs expressed in the somatic cells of model organisms provide a versatile tool for functional genomics.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree false
49 schema:isPartOf Na555d83eac64429eb7ee4e39a3674c4e
50 Nb372d422193f418da137ff9cb27c802b
51 sg:journal.1115214
52 schema:name Conditional targeted genome editing using somatically expressed TALENs in C. elegans
53 schema:pagination 934-937
54 schema:productId N3a70152d0d7a495da1e0814d95b57846
55 N55eb227f2dd74248a6aca807dfb042e0
56 N6216f2c40631455aad89776217992a0d
57 Na2be4b4962824388b5a647a7a23aa4ff
58 Nf9890275f8e34cbd8b74112986c79049
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006594155
60 https://doi.org/10.1038/nbt.2674
61 schema:sdDatePublished 2019-04-11T01:46
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher N482ca345a5f24aec8a07fe32e5224dd1
64 schema:url http://www.nature.com/articles/nbt.2674
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N0071020127fc498fa218b348cc266fab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Cell Movement
70 rdf:type schema:DefinedTerm
71 N007e0a6c98164edb9eb66377c666ca7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Deoxyribonucleases
73 rdf:type schema:DefinedTerm
74 N2a378d0c595746ee84b42270852911f8 rdf:first sg:person.012610261547.89
75 rdf:rest Nbebc1919926847a688f62518d2e1680e
76 N2aa2b52d7dea48a08d2bad5239b40530 rdf:first sg:person.01345152476.92
77 rdf:rest N9fec6390ae7c497b8614d727ca0698cf
78 N2ca0d9eebb1e443ba237e5c5c6103e02 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Humans
80 rdf:type schema:DefinedTerm
81 N3a5ba8d6537444ebaa51fda5593e5a60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name RNA Editing
83 rdf:type schema:DefinedTerm
84 N3a70152d0d7a495da1e0814d95b57846 schema:name dimensions_id
85 schema:value pub.1006594155
86 rdf:type schema:PropertyValue
87 N46dbdcf9c5c44e7e9ba36c63f6af55ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Neurons
89 rdf:type schema:DefinedTerm
90 N482ca345a5f24aec8a07fe32e5224dd1 schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 N49946105f6d9472a815f0ef78f79b7c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Cell Lineage
94 rdf:type schema:DefinedTerm
95 N55eb227f2dd74248a6aca807dfb042e0 schema:name readcube_id
96 schema:value 1f687b1707bc3cb98e856a8059041b48583952f2b1338251a9bf27a2d63b1596
97 rdf:type schema:PropertyValue
98 N57b85c93d71e46408f151d013c56eebe rdf:first sg:person.01306337250.96
99 rdf:rest N2aa2b52d7dea48a08d2bad5239b40530
100 N5bbaec444bab4a36a5b95e07f4430b1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Mutation
102 rdf:type schema:DefinedTerm
103 N5f67b860c5e7411a8174b6a8804ae6a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Animals
105 rdf:type schema:DefinedTerm
106 N5fa5b98e375e4025bbc112d5831303bb rdf:first sg:person.016205376120.55
107 rdf:rest N93281b3ff4fa4d85ab8a537cf37d0269
108 N6216f2c40631455aad89776217992a0d schema:name doi
109 schema:value 10.1038/nbt.2674
110 rdf:type schema:PropertyValue
111 N733765684a4046ac9d1179462b9a1351 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Genome, Helminth
113 rdf:type schema:DefinedTerm
114 N8266b5b97dcf478f9480dce4b66520ae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Luminescent Proteins
116 rdf:type schema:DefinedTerm
117 N88715d12209544129e06074e65de99c9 rdf:first sg:person.01013410150.53
118 rdf:rest N2a378d0c595746ee84b42270852911f8
119 N93281b3ff4fa4d85ab8a537cf37d0269 rdf:first sg:person.01025562770.75
120 rdf:rest rdf:nil
121 N994d35ce47bd44caa972fb33fe160216 rdf:first sg:person.0777356210.30
122 rdf:rest N99505284f467400a8bca2adf1333bb63
123 N99505284f467400a8bca2adf1333bb63 rdf:first sg:person.01230724076.45
124 rdf:rest N57b85c93d71e46408f151d013c56eebe
125 N9fec6390ae7c497b8614d727ca0698cf rdf:first sg:person.01061523350.29
126 rdf:rest N88715d12209544129e06074e65de99c9
127 Na2be4b4962824388b5a647a7a23aa4ff schema:name nlm_unique_id
128 schema:value 9604648
129 rdf:type schema:PropertyValue
130 Na555d83eac64429eb7ee4e39a3674c4e schema:volumeNumber 31
131 rdf:type schema:PublicationVolume
132 Nb372d422193f418da137ff9cb27c802b schema:issueNumber 10
133 rdf:type schema:PublicationIssue
134 Nb753986b38504add82b61edbaa12ac8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Base Sequence
136 rdf:type schema:DefinedTerm
137 Nbebc1919926847a688f62518d2e1680e rdf:first sg:person.0667661576.34
138 rdf:rest N5fa5b98e375e4025bbc112d5831303bb
139 Nc0689bc32c4a43ac9abac26fe13ee85c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Caenorhabditis elegans
141 rdf:type schema:DefinedTerm
142 Nda27910f354b4c7d85b56b813de62032 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Trans-Activators
144 rdf:type schema:DefinedTerm
145 Nef47ebe8bf0a478eb05a118d7bc080af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Molecular Sequence Data
147 rdf:type schema:DefinedTerm
148 Nf741a64afc1547afa8d86549a9faec15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Green Fluorescent Proteins
150 rdf:type schema:DefinedTerm
151 Nf79c0f8c318841868b8f90c0229ed72c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Mechanotransduction, Cellular
153 rdf:type schema:DefinedTerm
154 Nf9890275f8e34cbd8b74112986c79049 schema:name pubmed_id
155 schema:value 23955274
156 rdf:type schema:PropertyValue
157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
158 schema:name Biological Sciences
159 rdf:type schema:DefinedTerm
160 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
161 schema:name Genetics
162 rdf:type schema:DefinedTerm
163 sg:grant.6992538 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt.2674
164 rdf:type schema:MonetaryGrant
165 sg:grant.7006596 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt.2674
166 rdf:type schema:MonetaryGrant
167 sg:grant.7006882 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt.2674
168 rdf:type schema:MonetaryGrant
169 sg:grant.7011788 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt.2674
170 rdf:type schema:MonetaryGrant
171 sg:grant.7019462 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt.2674
172 rdf:type schema:MonetaryGrant
173 sg:grant.7183558 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt.2674
174 rdf:type schema:MonetaryGrant
175 sg:journal.1115214 schema:issn 1087-0156
176 1546-1696
177 schema:name Nature Biotechnology
178 rdf:type schema:Periodical
179 sg:person.01013410150.53 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
180 schema:familyName Yang
181 schema:givenName Yihong
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013410150.53
183 rdf:type schema:Person
184 sg:person.01025562770.75 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
185 schema:familyName Ou
186 schema:givenName Guangshuo
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025562770.75
188 rdf:type schema:Person
189 sg:person.01061523350.29 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
190 schema:familyName Feng
191 schema:givenName Guoxin
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061523350.29
193 rdf:type schema:Person
194 sg:person.01230724076.45 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
195 schema:familyName Yi
196 schema:givenName Peishan
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230724076.45
198 rdf:type schema:Person
199 sg:person.012610261547.89 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
200 schema:familyName Liang
201 schema:givenName Xing
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012610261547.89
203 rdf:type schema:Person
204 sg:person.01306337250.96 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
205 schema:familyName Wang
206 schema:givenName Xiangming
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306337250.96
208 rdf:type schema:Person
209 sg:person.01345152476.92 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
210 schema:familyName Chai
211 schema:givenName Yongping
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345152476.92
213 rdf:type schema:Person
214 sg:person.016205376120.55 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
215 schema:familyName Li
216 schema:givenName Wei
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016205376120.55
218 rdf:type schema:Person
219 sg:person.0667661576.34 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
220 schema:familyName Zhu
221 schema:givenName Zhiwen
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667661576.34
223 rdf:type schema:Person
224 sg:person.0777356210.30 schema:affiliation https://www.grid.ac/institutes/grid.11135.37
225 schema:familyName Cheng
226 schema:givenName Ze
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777356210.30
228 rdf:type schema:Person
229 sg:pub.10.1007/s00018-006-6012-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1027216176
230 https://doi.org/10.1007/s00018-006-6012-z
231 rdf:type schema:CreativeWork
232 sg:pub.10.1038/nbt.1755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016004947
233 https://doi.org/10.1038/nbt.1755
234 rdf:type schema:CreativeWork
235 sg:pub.10.1038/nbt.1939 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010610351
236 https://doi.org/10.1038/nbt.1939
237 rdf:type schema:CreativeWork
238 sg:pub.10.1038/ni.1662 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028044442
239 https://doi.org/10.1038/ni.1662
240 rdf:type schema:CreativeWork
241 sg:pub.10.1038/nmeth.1454 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023315310
242 https://doi.org/10.1038/nmeth.1454
243 rdf:type schema:CreativeWork
244 sg:pub.10.1038/nmeth.1463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032555226
245 https://doi.org/10.1038/nmeth.1463
246 rdf:type schema:CreativeWork
247 sg:pub.10.1038/nmeth.1539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010147854
248 https://doi.org/10.1038/nmeth.1539
249 rdf:type schema:CreativeWork
250 sg:pub.10.1038/nmeth0710-488a schema:sameAs https://app.dimensions.ai/details/publication/pub.1038282017
251 https://doi.org/10.1038/nmeth0710-488a
252 rdf:type schema:CreativeWork
253 sg:pub.10.1038/nprot.2012.128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038902182
254 https://doi.org/10.1038/nprot.2012.128
255 rdf:type schema:CreativeWork
256 sg:pub.10.1038/nrm3486 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050461034
257 https://doi.org/10.1038/nrm3486
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1002/j.1460-2075.1991.tb04966.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077884622
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1016/0092-8674(86)90668-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022604578
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1016/j.cub.2006.11.065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022245162
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1016/j.stem.2012.11.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030194691
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1016/j.tcb.2011.04.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041640330
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1091/mbc.3.2.221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017206456
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1126/science.1130563 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035876594
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1126/science.1207773 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062464819
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1126/science.1226201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048506578
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1371/journal.pgen.1000028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049457203
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1371/journal.pone.0016644 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051334047
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1534/g3.112.003830 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028372231
282 rdf:type schema:CreativeWork
283 https://doi.org/10.4049/jimmunol.1003352 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038120373
284 rdf:type schema:CreativeWork
285 https://www.grid.ac/institutes/grid.11135.37 schema:alternateName Peking University
286 schema:name National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China.
287 School of Life Sciences, Peking University, Beijing, China.
288 rdf:type schema:Organization
289 https://www.grid.ac/institutes/grid.418856.6 schema:alternateName Institute of Biophysics
290 schema:name National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China.
291 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...