Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-03

AUTHORS

Seung Woo Cho, Sojung Kim, Jong Min Kim, Jin-Soo Kim

ABSTRACT

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%. More... »

PAGES

230

Journal

TITLE

Nature Biotechnology

ISSUE

3

VOLUME

31

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.2507

    DOI

    http://dx.doi.org/10.1038/nbt.2507

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1041881936

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23360966


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