Genetic engineering of human pluripotent cells using TALE nucleases View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-08

AUTHORS

Dirk Hockemeyer, Haoyi Wang, Samira Kiani, Christine S Lai, Qing Gao, John P Cassady, Gregory J Cost, Lei Zhang, Yolanda Santiago, Jeffrey C Miller, Bryan Zeitler, Jennifer M Cherone, Xiangdong Meng, Sarah J Hinkley, Edward J Rebar, Philip D Gregory, Fyodor D Urnov, Rudolf Jaenisch

ABSTRACT

Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs). More... »

PAGES

731

Journal

TITLE

Nature Biotechnology

ISSUE

8

VOLUME

29

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.1927

    DOI

    http://dx.doi.org/10.1038/nbt.1927

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1047844983

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21738127


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