Ontology type: schema:ScholarlyArticle Open Access: True
2011-01
AUTHORSEirini P Papapetrou, Gabsang Lee, Nirav Malani, Manu Setty, Isabelle Riviere, Laxmi M S Tirunagari, Kyuichi Kadota, Shoshannah L Roth, Patricia Giardina, Agnes Viale, Christina Leslie, Frederic D Bushman, Lorenz Studer, Michel Sadelain
ABSTRACTRealizing the therapeutic potential of human induced pluripotent stem (iPS) cells will require robust, precise and safe strategies for genetic modification, as cell therapies that rely on randomly integrated transgenes pose oncogenic risks. Here we describe a strategy to genetically modify human iPS cells at 'safe harbor' sites in the genome, which fulfill five criteria based on their position relative to contiguous coding genes, microRNAs and ultraconserved regions. We demonstrate that ∼10% of integrations of a lentivirally encoded β-globin transgene in β-thalassemia-patient iPS cell clones meet our safe harbor criteria and permit high-level β-globin expression upon erythroid differentiation without perturbation of neighboring gene expression. This approach, combining bioinformatics and functional analyses, should be broadly applicable to introducing therapeutic or suicide genes into patient-specific iPS cells for use in cell therapy. More... »
PAGES73
http://scigraph.springernature.com/pub.10.1038/nbt.1717
DOIhttp://dx.doi.org/10.1038/nbt.1717
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1007507053
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/21151124
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Differentiation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Erythroid Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Female",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Engineering",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Therapy",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Vectors",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genome, Human",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Induced Pluripotent Stem Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lentivirus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Male",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transgenes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "beta-Globins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "beta-Thalassemia",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Center for Cell Engineering, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.",
"Molecular Pharmacology and Chemistry Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Papapetrou",
"givenName": "Eirini P",
"id": "sg:person.01175455717.64",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175455717.64"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Lee",
"givenName": "Gabsang",
"id": "sg:person.01362764045.30",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362764045.30"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Pennsylvania",
"id": "https://www.grid.ac/institutes/grid.25879.31",
"name": [
"Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA."
],
"type": "Organization"
},
"familyName": "Malani",
"givenName": "Nirav",
"id": "sg:person.0667160756.42",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667160756.42"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Computational Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Setty",
"givenName": "Manu",
"id": "sg:person.01241212163.91",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241212163.91"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Center for Cell Engineering, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.",
"Molecular Pharmacology and Chemistry Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.",
"Cell Therapy and Cell Engineering Facility, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Riviere",
"givenName": "Isabelle",
"id": "sg:person.0725625656.58",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725625656.58"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Center for Cell Engineering, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.",
"Molecular Pharmacology and Chemistry Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Tirunagari",
"givenName": "Laxmi M S",
"id": "sg:person.01321567405.81",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321567405.81"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Center for Cell Engineering, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.",
"Thoracic Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Kadota",
"givenName": "Kyuichi",
"id": "sg:person.01172565026.16",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172565026.16"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Pennsylvania",
"id": "https://www.grid.ac/institutes/grid.25879.31",
"name": [
"Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA."
],
"type": "Organization"
},
"familyName": "Roth",
"givenName": "Shoshannah L",
"id": "sg:person.012324671157.45",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012324671157.45"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Cornell University",
"id": "https://www.grid.ac/institutes/grid.5386.8",
"name": [
"Thalassemia Program, Pediatric Hematology/Oncology Division, Weill Cornell Medical College, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Giardina",
"givenName": "Patricia",
"id": "sg:person.014644337477.48",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014644337477.48"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Genomics Core Facility, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Viale",
"givenName": "Agnes",
"id": "sg:person.0712021032.88",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0712021032.88"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Computational Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Leslie",
"givenName": "Christina",
"id": "sg:person.01250016667.26",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250016667.26"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Pennsylvania",
"id": "https://www.grid.ac/institutes/grid.25879.31",
"name": [
"Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA."
],
"type": "Organization"
},
"familyName": "Bushman",
"givenName": "Frederic D",
"id": "sg:person.01216060406.79",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216060406.79"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Center for Cell Engineering, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.",
"Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Studer",
"givenName": "Lorenz",
"id": "sg:person.0642253700.03",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0642253700.03"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Memorial Sloan Kettering Cancer Center",
"id": "https://www.grid.ac/institutes/grid.51462.34",
"name": [
"Center for Cell Engineering, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.",
"Molecular Pharmacology and Chemistry Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA."
],
"type": "Organization"
},
"familyName": "Sadelain",
"givenName": "Michel",
"id": "sg:person.01166755103.15",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01166755103.15"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1126/science.1152092",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000037346"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.stem.2009.05.023",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000372960"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci35798",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004730233"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/stem.257",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009536162"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0092-8674(02)00864-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010538565"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302593",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010556671",
"https://doi.org/10.1038/sj.gt.3302593"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302593",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010556671",
"https://doi.org/10.1038/sj.gt.3302593"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302593",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010556671",
"https://doi.org/10.1038/sj.gt.3302593"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cell.2007.11.019",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010904856"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature06534",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011992920",
"https://doi.org/10.1038/nature06534"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gr.6286907",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013262912"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.1516",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017504047",
"https://doi.org/10.1038/nbt.1516"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1088547",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018519577"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth1103",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018982307",
"https://doi.org/10.1038/nmeth1103"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35017565",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021691346",
"https://doi.org/10.1038/35017565"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35017565",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021691346",
"https://doi.org/10.1038/35017565"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1098119",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022514256"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gr.229102",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022792016"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature08129",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023609915",
"https://doi.org/10.1038/nature08129"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature08129",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023609915",
"https://doi.org/10.1038/nature08129"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1182/blood-2005-11-011874",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023657343"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci31666",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023711644"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1310",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024578139",
"https://doi.org/10.1038/nbt1310"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1182/blood-2007-07-102087",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027358004"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/bioinformatics/btl646",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031224678"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1182/blood-2009-11-252650",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032995132"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.1562",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033826570",
"https://doi.org/10.1038/nbt.1562"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.1562",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033826570",
"https://doi.org/10.1038/nbt.1562"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci31661",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035515942"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.stem.2008.04.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038035516"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci37216",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038425205"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci31659",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042616999"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nm1393",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043834387",
"https://doi.org/10.1038/nm1393"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nm1393",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043834387",
"https://doi.org/10.1038/nm1393"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature08320",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044017606",
"https://doi.org/10.1038/nature08320"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature08320",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044017606",
"https://doi.org/10.1038/nature08320"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.0904825106",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045086889"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1362",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045517508",
"https://doi.org/10.1038/nbt1362"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.78.6.2906-2920.2004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045733077"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2202/1544-6115.1008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047832492"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1151526",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048905674"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature09328",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049299780",
"https://doi.org/10.1038/nature09328"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1634/stemcells.2007-0039",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049467439"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1634/stemcells.2008-0642",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051271009"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1105063",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062451191"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/156652408786241357",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069185949"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2202/1544-6115.1027",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069289261"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078880365",
"type": "CreativeWork"
}
],
"datePublished": "2011-01",
"datePublishedReg": "2011-01-01",
"description": "Realizing the therapeutic potential of human induced pluripotent stem (iPS) cells will require robust, precise and safe strategies for genetic modification, as cell therapies that rely on randomly integrated transgenes pose oncogenic risks. Here we describe a strategy to genetically modify human iPS cells at 'safe harbor' sites in the genome, which fulfill five criteria based on their position relative to contiguous coding genes, microRNAs and ultraconserved regions. We demonstrate that \u223c10% of integrations of a lentivirally encoded \u03b2-globin transgene in \u03b2-thalassemia-patient iPS cell clones meet our safe harbor criteria and permit high-level \u03b2-globin expression upon erythroid differentiation without perturbation of neighboring gene expression. This approach, combining bioinformatics and functional analyses, should be broadly applicable to introducing therapeutic or suicide genes into patient-specific iPS cells for use in cell therapy.",
"genre": "research_article",
"id": "sg:pub.10.1038/nbt.1717",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.2458614",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2456012",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2436886",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1115214",
"issn": [
"1087-0156",
"1546-1696"
],
"name": "Nature Biotechnology",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "29"
}
],
"name": "Genomic safe harbors permit high \u03b2-globin transgene expression in thalassemia induced pluripotent stem cells",
"pagination": "73",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"23c1feb42d52f98f3e99b8f13da3f0d9d3bb6ed8ac5b35f40b33625d1d821af1"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"21151124"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9604648"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/nbt.1717"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1007507053"
]
}
],
"sameAs": [
"https://doi.org/10.1038/nbt.1717",
"https://app.dimensions.ai/details/publication/pub.1007507053"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T14:49",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000435.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/nbt.1717"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt.1717'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt.1717'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt.1717'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt.1717'
This table displays all metadata directly associated to this object as RDF triples.
384 TRIPLES
21 PREDICATES
88 URIs
39 LITERALS
27 BLANK NODES