Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511 View Full Text


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Article Info

DATE

2010-06

AUTHORS

Sergey Rodin, Anna Domogatskaya, Susanne Ström, Emil M Hansson, Kenneth R Chien, José Inzunza, Outi Hovatta, Karl Tryggvason

ABSTRACT

We describe a system for culturing human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells on a recombinant form of human laminin-511, a component of the natural hES cell niche. The system is devoid of animal products and feeder cells and contains only one undefined component, human albumin. The hES cells self-renewed with normal karyotype for at least 4 months (20 passages), after which the cells could produce teratomas containing cell lineages of all three germ layers. When plated on laminin-511 in small clumps, hES cells spread out in a monolayer, maintaining cellular homogeneity with approximately 97% OCT4-positive cells. Adhesion of hES cells was dependent on alpha6beta1 integrin. The use of homogeneous monolayer hES or iPS cell cultures provides more controllable conditions for the design of differentiation methods. This xeno-free and feeder-free system may be useful for the development of cell lineages for therapeutic purposes. More... »

PAGES

611

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt.1620

DOI

http://dx.doi.org/10.1038/nbt.1620

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019661466

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20512123


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