Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-11

AUTHORS

Elayne M Chan, Sutheera Ratanasirintrawoot, In-Hyun Park, Philip D Manos, Yuin-Han Loh, Hongguang Huo, Justine D Miller, Odelya Hartung, Junsung Rho, Tan A Ince, George Q Daley, Thorsten M Schlaeger

ABSTRACT

Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by enforced expression of transcription factors. Using serial live imaging of human fibroblasts undergoing reprogramming, we identified distinct colony types that morphologically resemble embryonic stem (ES) cells yet differ in molecular phenotype and differentiation potential. By analyzing expression of pluripotency markers, methylation at the OCT4 and NANOG promoters and differentiation into teratomas, we determined that only one colony type represents true iPS cells, whereas the others represent reprogramming intermediates. Proviral silencing and expression of TRA-1-60, DNMT3B and REX1 can be used to distinguish the fully reprogrammed state, whereas alkaline phosphatase, SSEA-4, GDF3, hTERT and NANOG are insufficient as markers. We also show that reprogramming using chemically defined medium favors formation of fully reprogrammed over partially reprogrammed colonies. Our data define molecular markers of the fully reprogrammed state and highlight the need for rigorous characterization and standardization of putative iPS cells. More... »

PAGES

1033

Journal

TITLE

Nature Biotechnology

ISSUE

11

VOLUME

27

Related Patents

  • Selection Method Of Induced Pluripotent Stem Cells
  • Induced Pluripotent Stem Cells With Synthetic Modified Rnas
  • Kit For Making Induced Pluripotent Stem Cells Using Modified Rnas
  • Compositions And Methods For Tissue Engineering, Tissue Regeneration And Wound Healing
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Regulating Transplant Rejection Of Donor And Embryonic Stem Cell-Derived Tissues And Organs
  • Engineered Nucleic Acids Encoding A Modified Erythropoietin And Their Expression
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Modified Polynucleotides Encoding Granulysin
  • Controlled Cell Delivery Vehicle And Treatment Of Tumours
  • Modified Polynucleotides For Treating Galactosylceramidase Protein Deficiency
  • Detection Of Human Somatic Cell Reprogramming
  • Method Of Efficiently Establishing Induced Pluripotent Stem Cells
  • Protein-Induced Pluripotent Cell Technology And Uses Thereof
  • Compositions, Kits, And Methods For Making Induced Pluripotent Stem Cells Using Synthetic Modified Rnas
  • Detection Of Human Somatic Cell Reprogramming
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Regulating Transplant Rejection Of Donor And Embryonic Stem Cell-Derived Tissues And Organs
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Detection Of Human Somatic Cell Reprogramming
  • Modified Polynucleotides Encoding Cited4
  • Modified Polynucleotides For The Production Of Nuclear Proteins
  • Modified Polynucleotides Encoding Septin-4
  • Kits Comprising Linear Dnas For Sustained Polypeptide Expression Using Synthetic, Modified Rnas
  • Compositions And Methods For Reprogramming Eukaryotic Cells
  • Compositions And Methods For Differentiating Stem Cells Into Cell Populations Comprising Beta-Like Cells
  • Modified Polynucleotides For Treating Protein Deficiency
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Apoptosis Inducing Factor 1
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Modified Polynucleotides For The Production Of Proteins Associated With Human Disease
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Copper Metabolism (Murr1) Domain Containing 1
  • Primate Pluripotent Stem Cells Cultured In Medium Containing Gamma-Aminobutyric Acid, Pipecolic Acid And Lithium
  • Making And Using In Vitro-Synthesized Ssrna For Introducing Into Mammalian Cells To Induce A Biological Or Biochemical Effect
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Formulation And Delivery Of Plga Microspheres
  • Compositions And Methods For Reprogramming Eukaryotic Cells
  • Compositions And Methods Of Altering Cholesterol Levels
  • Modified Polynucleotides Encoding Basic Helix-Loop-Helix Family Member E41
  • Modified Polynucleotides For The Production Of Proteins
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Oncology-Related Proteins And Peptides
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of G-Csf
  • In Vitro Model Of Spinal Muscular Atrophy
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Cell Modification And Application In Therapy
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Terminally Modified Rna
  • Methods, Systems And Compositions Relating To Cell Conversion Via Protein-Induced In-Vivo Cell Reprogramming
  • Modified Polynucleotides For The Production Of Cosmetic Proteins And Peptides
  • Making And Using In Vitro-Synthesized Ssrna For Introducing Into Mammalian Cells To Induce A Biological Or Biochemical Effect
  • Compositions And Methods For Differentiating Stem Cells Into Cell Populations Comprising Beta-Like Cells
  • Compositions And Methods For Tissue Engineering, Tissue Regeneration And Wound Healing
  • In Vivo Production Of Proteins
  • Modified Polynucleotides Encoding Siah E3 Ubiquitin Protein Ligase 1
  • Modified Polynucleotides Encoding Hepatitis A Virus Cellular Receptor 2
  • Dlin-Kc2-Dma Lipid Nanoparticle Delivery Of Modified Polynucleotides
  • Method For De-Differentiating A Cell
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Selection Method Of Induced Pluripotent Stem Cells
  • Modified Polynucleotides For The Production Of Proteins
  • Therapeutic And Diagnostic Methods And Compositions For Neurodegenerative Diseases
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Method For Producing A Protein Of Interest In A Primate
  • Modified Polynucleotides Encoding Aquaporin-5
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Methods For Producing Insulin-Secreting Beta Cells From Human Pluripotent Stem Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Split Dose Administration
  • An Automated System For The Cultivation And Differentiation Pluripotent Stem Cells
  • Modified Polynucleotides Encoding Aryl Hydrocarbon Receptor Nuclear Translocator
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Markers For Differentiation Of Stem Cells Into Differentiated Cell Populations
  • Quality Determination Of Stem Cells
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Formulation And Delivery Of Plga Microspheres
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.1580

    DOI

    http://dx.doi.org/10.1038/nbt.1580

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1052579867

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19826408


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Shape", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Survival", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cellular Reprogramming", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Colony-Forming Units Assay", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Embryonic Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fibroblasts", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Flow Cytometry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fluorescent Antibody Technique", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Imaging, Three-Dimensional", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Induced Pluripotent Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Teratoma", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Time Factors", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chan", 
            "givenName": "Elayne M", 
            "id": "sg:person.0635431646.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635431646.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA.", 
                "Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ratanasirintrawoot", 
            "givenName": "Sutheera", 
            "id": "sg:person.0602234506.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602234506.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Park", 
            "givenName": "In-Hyun", 
            "id": "sg:person.0633264666.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633264666.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Boston Children's Hospital", 
              "id": "https://www.grid.ac/institutes/grid.2515.3", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Manos", 
            "givenName": "Philip D", 
            "id": "sg:person.01325640606.07", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325640606.07"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Loh", 
            "givenName": "Yuin-Han", 
            "id": "sg:person.0613013726.07", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613013726.07"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Boston Children's Hospital", 
              "id": "https://www.grid.ac/institutes/grid.2515.3", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Huo", 
            "givenName": "Hongguang", 
            "id": "sg:person.01206166374.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206166374.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Boston Children's Hospital", 
              "id": "https://www.grid.ac/institutes/grid.2515.3", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Miller", 
            "givenName": "Justine D", 
            "id": "sg:person.01307060732.61", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307060732.61"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Boston Children's Hospital", 
              "id": "https://www.grid.ac/institutes/grid.2515.3", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hartung", 
            "givenName": "Odelya", 
            "id": "sg:person.0673526604.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673526604.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Dana\u2013Farber Cancer Institute", 
              "id": "https://www.grid.ac/institutes/grid.65499.37", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rho", 
            "givenName": "Junsung", 
            "id": "sg:person.01137626510.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137626510.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Brigham and Women's Hospital", 
              "id": "https://www.grid.ac/institutes/grid.62560.37", 
              "name": [
                "Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ince", 
            "givenName": "Tan A", 
            "id": "sg:person.01233401714.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233401714.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Boston Children's Hospital", 
              "id": "https://www.grid.ac/institutes/grid.2515.3", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.", 
                "Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA.", 
                "Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.", 
                "Howard Hughes Medical Institute at Children's Hospital Boston, Boston, Massachusetts, USA.", 
                "Division of Hematology/Oncology, Brigham and Women's Hospital, Boston, Massachusetts, USA.", 
                "Manton Center for Orphan Disease Research, Children's Hospital Boston, Boston, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Daley", 
            "givenName": "George Q", 
            "id": "sg:person.01326207001.66", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326207001.66"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.", 
                "Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA.", 
                "Harvard Stem Cell Institute, Cambridge, Massachusetts, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schlaeger", 
            "givenName": "Thorsten M", 
            "id": "sg:person.0764576306.77", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764576306.77"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.stem.2008.11.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001426359"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05934", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002347899", 
              "https://doi.org/10.1038/nature05934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2008.92", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003864180", 
              "https://doi.org/10.1038/nprot.2008.92"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.08.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004948652"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2009.02.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005033722"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.02.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008490443"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2007.11.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010904856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbamem.2008.08.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011092568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06534", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011992920", 
              "https://doi.org/10.1038/nature06534"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014573758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05944", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019109862", 
              "https://doi.org/10.1038/nature05944"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0711983105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024490582"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.05.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029649252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncb1827", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035894206", 
              "https://doi.org/10.1038/ncb1827"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2009.02.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039036557"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1158799", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039463429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.08.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040008680"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1172482", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044377678"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2008.07.041", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047970638"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1151526", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048905674"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.01.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053248333"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1164270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062458687"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2009-11", 
        "datePublishedReg": "2009-11-01", 
        "description": "Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by enforced expression of transcription factors. Using serial live imaging of human fibroblasts undergoing reprogramming, we identified distinct colony types that morphologically resemble embryonic stem (ES) cells yet differ in molecular phenotype and differentiation potential. By analyzing expression of pluripotency markers, methylation at the OCT4 and NANOG promoters and differentiation into teratomas, we determined that only one colony type represents true iPS cells, whereas the others represent reprogramming intermediates. Proviral silencing and expression of TRA-1-60, DNMT3B and REX1 can be used to distinguish the fully reprogrammed state, whereas alkaline phosphatase, SSEA-4, GDF3, hTERT and NANOG are insufficient as markers. We also show that reprogramming using chemically defined medium favors formation of fully reprogrammed over partially reprogrammed colonies. Our data define molecular markers of the fully reprogrammed state and highlight the need for rigorous characterization and standardization of putative iPS cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt.1580", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "11", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "27"
          }
        ], 
        "name": "Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells", 
        "pagination": "1033", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "fa747e9b89d585f9da89c0f98ab5ec14a379fe2b6462449905cbe43fba461d18"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "19826408"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt.1580"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1052579867"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt.1580", 
          "https://app.dimensions.ai/details/publication/pub.1052579867"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T09:31", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99803_00000003.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nbt.1580"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt.1580'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt.1580'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt.1580'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt.1580'


     

    This table displays all metadata directly associated to this object as RDF triples.

    296 TRIPLES      21 PREDICATES      66 URIs      36 LITERALS      24 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt.1580 schema:about N2ebb3d7cfe124009a47391ca7d9cf214
    2 N3c253b09d50547059984cca254fa2796
    3 N3e6fcbc6207045d897f2a03cf0b85d13
    4 N504f95438ea54dcf997e688f03fda24a
    5 N93febe2b5df348e19dfaa7e7d639c89f
    6 N943a68c31288497985f978699f0f30b7
    7 N9c073b2e9b4545369b90e9ed49baa69c
    8 Na94244612a3241edb682604675bc5a24
    9 Na9d2d90996464720bc9e9783b347b936
    10 Nc2d85c250c0b4852a1afe677aabef411
    11 Nc8a85446e82e4b32ab2110c9a426707d
    12 Nced1066eb30c49afbfe501304c3b3eb3
    13 Ndecdcff15d4a4f80a60a039e6bc15cf2
    14 Ne7dd3791339e4b40a49cb7c9e9217e08
    15 Neaf364f5d5a149d5918d3307ae640288
    16 anzsrc-for:06
    17 anzsrc-for:0604
    18 schema:author N5cd3bc0230e94eeaaf11f9862b6622d6
    19 schema:citation sg:pub.10.1038/nature05934
    20 sg:pub.10.1038/nature05944
    21 sg:pub.10.1038/nature06534
    22 sg:pub.10.1038/ncb1827
    23 sg:pub.10.1038/nprot.2008.92
    24 https://doi.org/10.1016/j.bbamem.2008.08.010
    25 https://doi.org/10.1016/j.cell.2006.07.024
    26 https://doi.org/10.1016/j.cell.2007.11.019
    27 https://doi.org/10.1016/j.cell.2008.07.041
    28 https://doi.org/10.1016/j.stem.2007.05.014
    29 https://doi.org/10.1016/j.stem.2008.01.004
    30 https://doi.org/10.1016/j.stem.2008.02.001
    31 https://doi.org/10.1016/j.stem.2008.08.003
    32 https://doi.org/10.1016/j.stem.2008.08.014
    33 https://doi.org/10.1016/j.stem.2008.11.008
    34 https://doi.org/10.1016/j.stem.2009.02.009
    35 https://doi.org/10.1016/j.stem.2009.02.010
    36 https://doi.org/10.1073/pnas.0711983105
    37 https://doi.org/10.1126/science.1151526
    38 https://doi.org/10.1126/science.1158799
    39 https://doi.org/10.1126/science.1164270
    40 https://doi.org/10.1126/science.1172482
    41 schema:datePublished 2009-11
    42 schema:datePublishedReg 2009-11-01
    43 schema:description Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by enforced expression of transcription factors. Using serial live imaging of human fibroblasts undergoing reprogramming, we identified distinct colony types that morphologically resemble embryonic stem (ES) cells yet differ in molecular phenotype and differentiation potential. By analyzing expression of pluripotency markers, methylation at the OCT4 and NANOG promoters and differentiation into teratomas, we determined that only one colony type represents true iPS cells, whereas the others represent reprogramming intermediates. Proviral silencing and expression of TRA-1-60, DNMT3B and REX1 can be used to distinguish the fully reprogrammed state, whereas alkaline phosphatase, SSEA-4, GDF3, hTERT and NANOG are insufficient as markers. We also show that reprogramming using chemically defined medium favors formation of fully reprogrammed over partially reprogrammed colonies. Our data define molecular markers of the fully reprogrammed state and highlight the need for rigorous characterization and standardization of putative iPS cells.
    44 schema:genre research_article
    45 schema:inLanguage en
    46 schema:isAccessibleForFree false
    47 schema:isPartOf N0b82e84e25fd4ab8b8b217a4bd014a02
    48 Neafb46b92d8e47a48a0e4a3c88f48628
    49 sg:journal.1115214
    50 schema:name Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells
    51 schema:pagination 1033
    52 schema:productId N9952f71143c64f708106a247b794e349
    53 N9a1bd0cdb08f4d7ea2e42f826a40c9c5
    54 Naa508f47e8c14017955c433c27f3ca8e
    55 Nf08c1eff5fa3495cb8d54bf08c3fccef
    56 Nf1cb23b693164b549ef0726ba66b11ef
    57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052579867
    58 https://doi.org/10.1038/nbt.1580
    59 schema:sdDatePublished 2019-04-11T09:31
    60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    61 schema:sdPublisher N7fd64f88503e49f09a84a67850bfd3c8
    62 schema:url https://www.nature.com/articles/nbt.1580
    63 sgo:license sg:explorer/license/
    64 sgo:sdDataset articles
    65 rdf:type schema:ScholarlyArticle
    66 N0b82e84e25fd4ab8b8b217a4bd014a02 schema:volumeNumber 27
    67 rdf:type schema:PublicationVolume
    68 N11209b13e2484ffd8bcb5f9b83b62f12 rdf:first sg:person.0673526604.70
    69 rdf:rest N72cb5512345c4215b5274ac848ea7dcf
    70 N14b814b119de4f54ad1fa26a1d5d7161 rdf:first sg:person.01307060732.61
    71 rdf:rest N11209b13e2484ffd8bcb5f9b83b62f12
    72 N25cf127cb5cc429ca87b6b24c2be83f0 rdf:first sg:person.0602234506.30
    73 rdf:rest Nd94691c56b78420aacac5b5210098287
    74 N2ebb3d7cfe124009a47391ca7d9cf214 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    75 schema:name Humans
    76 rdf:type schema:DefinedTerm
    77 N3a0d9d3390714aa0b14350a3292c9361 rdf:first sg:person.0613013726.07
    78 rdf:rest N49669b2f0e79439aa18001261244662e
    79 N3c253b09d50547059984cca254fa2796 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Embryonic Stem Cells
    81 rdf:type schema:DefinedTerm
    82 N3e6fcbc6207045d897f2a03cf0b85d13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    83 schema:name Cell Differentiation
    84 rdf:type schema:DefinedTerm
    85 N49669b2f0e79439aa18001261244662e rdf:first sg:person.01206166374.49
    86 rdf:rest N14b814b119de4f54ad1fa26a1d5d7161
    87 N504f95438ea54dcf997e688f03fda24a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Teratoma
    89 rdf:type schema:DefinedTerm
    90 N5bd603d2a50a413fad67199d79d6d125 rdf:first sg:person.0764576306.77
    91 rdf:rest rdf:nil
    92 N5cd3bc0230e94eeaaf11f9862b6622d6 rdf:first sg:person.0635431646.09
    93 rdf:rest N25cf127cb5cc429ca87b6b24c2be83f0
    94 N72cb5512345c4215b5274ac848ea7dcf rdf:first sg:person.01137626510.00
    95 rdf:rest N8a9a45935fdd472c815f6475747f1890
    96 N7fd64f88503e49f09a84a67850bfd3c8 schema:name Springer Nature - SN SciGraph project
    97 rdf:type schema:Organization
    98 N8a9a45935fdd472c815f6475747f1890 rdf:first sg:person.01233401714.29
    99 rdf:rest N9bb16652daa041e08187f36e82f7ed57
    100 N93febe2b5df348e19dfaa7e7d639c89f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Cell Shape
    102 rdf:type schema:DefinedTerm
    103 N943a68c31288497985f978699f0f30b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Imaging, Three-Dimensional
    105 rdf:type schema:DefinedTerm
    106 N97fda88589de4eb7b2b6b5694b3968d7 rdf:first sg:person.01325640606.07
    107 rdf:rest N3a0d9d3390714aa0b14350a3292c9361
    108 N9952f71143c64f708106a247b794e349 schema:name dimensions_id
    109 schema:value pub.1052579867
    110 rdf:type schema:PropertyValue
    111 N9a1bd0cdb08f4d7ea2e42f826a40c9c5 schema:name nlm_unique_id
    112 schema:value 9604648
    113 rdf:type schema:PropertyValue
    114 N9bb16652daa041e08187f36e82f7ed57 rdf:first sg:person.01326207001.66
    115 rdf:rest N5bd603d2a50a413fad67199d79d6d125
    116 N9c073b2e9b4545369b90e9ed49baa69c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Flow Cytometry
    118 rdf:type schema:DefinedTerm
    119 Na94244612a3241edb682604675bc5a24 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Time Factors
    121 rdf:type schema:DefinedTerm
    122 Na9d2d90996464720bc9e9783b347b936 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Fibroblasts
    124 rdf:type schema:DefinedTerm
    125 Naa508f47e8c14017955c433c27f3ca8e schema:name doi
    126 schema:value 10.1038/nbt.1580
    127 rdf:type schema:PropertyValue
    128 Nc2d85c250c0b4852a1afe677aabef411 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Cell Survival
    130 rdf:type schema:DefinedTerm
    131 Nc8a85446e82e4b32ab2110c9a426707d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Colony-Forming Units Assay
    133 rdf:type schema:DefinedTerm
    134 Nced1066eb30c49afbfe501304c3b3eb3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Cell Line
    136 rdf:type schema:DefinedTerm
    137 Nd94691c56b78420aacac5b5210098287 rdf:first sg:person.0633264666.40
    138 rdf:rest N97fda88589de4eb7b2b6b5694b3968d7
    139 Ndecdcff15d4a4f80a60a039e6bc15cf2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Cellular Reprogramming
    141 rdf:type schema:DefinedTerm
    142 Ne7dd3791339e4b40a49cb7c9e9217e08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Fluorescent Antibody Technique
    144 rdf:type schema:DefinedTerm
    145 Neaf364f5d5a149d5918d3307ae640288 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Induced Pluripotent Stem Cells
    147 rdf:type schema:DefinedTerm
    148 Neafb46b92d8e47a48a0e4a3c88f48628 schema:issueNumber 11
    149 rdf:type schema:PublicationIssue
    150 Nf08c1eff5fa3495cb8d54bf08c3fccef schema:name readcube_id
    151 schema:value fa747e9b89d585f9da89c0f98ab5ec14a379fe2b6462449905cbe43fba461d18
    152 rdf:type schema:PropertyValue
    153 Nf1cb23b693164b549ef0726ba66b11ef schema:name pubmed_id
    154 schema:value 19826408
    155 rdf:type schema:PropertyValue
    156 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    157 schema:name Biological Sciences
    158 rdf:type schema:DefinedTerm
    159 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    160 schema:name Genetics
    161 rdf:type schema:DefinedTerm
    162 sg:journal.1115214 schema:issn 1087-0156
    163 1546-1696
    164 schema:name Nature Biotechnology
    165 rdf:type schema:Periodical
    166 sg:person.01137626510.00 schema:affiliation https://www.grid.ac/institutes/grid.65499.37
    167 schema:familyName Rho
    168 schema:givenName Junsung
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137626510.00
    170 rdf:type schema:Person
    171 sg:person.01206166374.49 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
    172 schema:familyName Huo
    173 schema:givenName Hongguang
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206166374.49
    175 rdf:type schema:Person
    176 sg:person.01233401714.29 schema:affiliation https://www.grid.ac/institutes/grid.62560.37
    177 schema:familyName Ince
    178 schema:givenName Tan A
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233401714.29
    180 rdf:type schema:Person
    181 sg:person.01307060732.61 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
    182 schema:familyName Miller
    183 schema:givenName Justine D
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307060732.61
    185 rdf:type schema:Person
    186 sg:person.01325640606.07 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
    187 schema:familyName Manos
    188 schema:givenName Philip D
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325640606.07
    190 rdf:type schema:Person
    191 sg:person.01326207001.66 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
    192 schema:familyName Daley
    193 schema:givenName George Q
    194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326207001.66
    195 rdf:type schema:Person
    196 sg:person.0602234506.30 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    197 schema:familyName Ratanasirintrawoot
    198 schema:givenName Sutheera
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602234506.30
    200 rdf:type schema:Person
    201 sg:person.0613013726.07 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    202 schema:familyName Loh
    203 schema:givenName Yuin-Han
    204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613013726.07
    205 rdf:type schema:Person
    206 sg:person.0633264666.40 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    207 schema:familyName Park
    208 schema:givenName In-Hyun
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633264666.40
    210 rdf:type schema:Person
    211 sg:person.0635431646.09 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    212 schema:familyName Chan
    213 schema:givenName Elayne M
    214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635431646.09
    215 rdf:type schema:Person
    216 sg:person.0673526604.70 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
    217 schema:familyName Hartung
    218 schema:givenName Odelya
    219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673526604.70
    220 rdf:type schema:Person
    221 sg:person.0764576306.77 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    222 schema:familyName Schlaeger
    223 schema:givenName Thorsten M
    224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764576306.77
    225 rdf:type schema:Person
    226 sg:pub.10.1038/nature05934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002347899
    227 https://doi.org/10.1038/nature05934
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1038/nature05944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019109862
    230 https://doi.org/10.1038/nature05944
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1038/nature06534 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011992920
    233 https://doi.org/10.1038/nature06534
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1038/ncb1827 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035894206
    236 https://doi.org/10.1038/ncb1827
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1038/nprot.2008.92 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003864180
    239 https://doi.org/10.1038/nprot.2008.92
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1016/j.bbamem.2008.08.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011092568
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1016/j.cell.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010904856
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1016/j.cell.2008.07.041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047970638
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1016/j.stem.2007.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029649252
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1016/j.stem.2008.01.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053248333
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1016/j.stem.2008.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008490443
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1016/j.stem.2008.08.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004948652
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1016/j.stem.2008.08.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040008680
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1016/j.stem.2008.11.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001426359
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1016/j.stem.2009.02.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039036557
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1016/j.stem.2009.02.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005033722
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1073/pnas.0711983105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024490582
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1126/science.1151526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048905674
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1126/science.1158799 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039463429
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1126/science.1164270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062458687
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1126/science.1172482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044377678
    274 rdf:type schema:CreativeWork
    275 https://www.grid.ac/institutes/grid.2515.3 schema:alternateName Boston Children's Hospital
    276 schema:name Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
    277 Division of Hematology/Oncology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
    278 Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
    279 Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
    280 Howard Hughes Medical Institute at Children's Hospital Boston, Boston, Massachusetts, USA.
    281 Manton Center for Orphan Disease Research, Children's Hospital Boston, Boston, Massachusetts, USA.
    282 Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA.
    283 rdf:type schema:Organization
    284 https://www.grid.ac/institutes/grid.38142.3c schema:alternateName Harvard University
    285 schema:name Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA.
    286 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
    287 Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
    288 Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
    289 Stem Cell Program, Children's Hospital Boston, Boston, Massachusetts, USA.
    290 rdf:type schema:Organization
    291 https://www.grid.ac/institutes/grid.62560.37 schema:alternateName Brigham and Women's Hospital
    292 schema:name Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
    293 rdf:type schema:Organization
    294 https://www.grid.ac/institutes/grid.65499.37 schema:alternateName Dana–Farber Cancer Institute
    295 schema:name Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
    296 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...