Sensitive digital quantification of DNA methylation in clinical samples View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-09

AUTHORS

Meng Li, Wei-dong Chen, Nickolas Papadopoulos, Steven N Goodman, Niels Christian Bjerregaard, Søren Laurberg, Bernard Levin, Hartmut Juhl, Nadir Arber, Helen Moinova, Kris Durkee, Kerstin Schmidt, Yiping He, Frank Diehl, Victor E Velculescu, Shibin Zhou, Luis A Diaz, Kenneth W Kinzler, Sanford D Markowitz, Bert Vogelstein

ABSTRACT

Analysis of abnormally methylated genes is increasingly important in basic research and in the development of cancer biomarkers. We have developed methyl-BEAMing technology to enable absolute quantification of the number of methylated molecules in a sample. Individual DNA fragments are amplified and analyzed either by flow cytometry or next-generation sequencing. We demonstrate enumeration of as few as one methylated molecule in approximately 5,000 unmethylated molecules in DNA from plasma or fecal samples. Using methylated vimentin as a biomarker in plasma samples, methyl-BEAMing detected 59% of cancer cases. In early-stage colorectal cancers, this sensitivity was four times more than that obtained by assaying serum-carcinoembryonic antigen (CEA). With stool samples, methyl-BEAMing detected 41% of cancers and 45% of advanced adenomas. In addition to diagnostic and prognostic applications, this digital quantification of rare methylation events should be applicable to preclinical assessment of new epigenetic biomarkers and quantitative analyses in epigenetic research. More... »

PAGES

858-863

References to SciGraph publications

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  • 2003-04. The power and the promise of DNA methylation markers in NATURE REVIEWS CANCER
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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.1559

    DOI

    http://dx.doi.org/10.1038/nbt.1559

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003456878

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19684580


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