Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-02

AUTHORS

Andreas Gnirke, Alexandre Melnikov, Jared Maguire, Peter Rogov, Emily M LeProust, William Brockman, Timothy Fennell, Georgia Giannoukos, Sheila Fisher, Carsten Russ, Stacey Gabriel, David B Jaffe, Eric S Lander, Chad Nusbaum

ABSTRACT

Targeting genomic loci by massively parallel sequencing requires new methods to enrich templates to be sequenced. We developed a capture method that uses biotinylated RNA 'baits' to fish targets out of a 'pond' of DNA fragments. The RNA is transcribed from PCR-amplified oligodeoxynucleotides originally synthesized on a microarray, generating sufficient bait for multiple captures at concentrations high enough to drive the hybridization. We tested this method with 170-mer baits that target >15,000 coding exons (2.5 Mb) and four regions (1.7 Mb total) using Illumina sequencing as read-out. About 90% of uniquely aligning bases fell on or near bait sequence; up to 50% lay on exons proper. The uniformity was such that approximately 60% of target bases in the exonic 'catch', and approximately 80% in the regional catch, had at least half the mean coverage. One lane of Illumina sequence was sufficient to call high-confidence genotypes for 89% of the targeted exon space. More... »

PAGES

182-189

Journal

TITLE

Nature Biotechnology

ISSUE

2

VOLUME

27

Related Patents

  • Selective Enrichment Of Cpg Islands
  • Method For The Simultaneous Amplification Of A Plurality Of Different Nucleic Acid Target Sequences
  • Full Interrogation Of Nuclease Dsbs And Sequencing (Find-Seq)
  • Means And Methods For Non-Invasive Diagnosis Of Chromosomal Aneuploidy
  • Determination Of The Depth Coverage Of The Fetal Genome
  • Optimization Of Multigene Analysis Of Tumor Samples
  • Partitioning Of Dna Sequencing Libraries Into Host And Microbial Components
  • Methods And Devices For Dna Sequencing And Molecular Diagnostics
  • Methods Of Treating Urothelial Carcinoma
  • Novel Method
  • Tm-Enhanced Blocking Oligonucleotides And Baits For Improved Target Enrichment And Reduced Off-Target Selection
  • Molecular Coding For Analysis Of Composition Of Macromolecules And Molecular Complexes
  • Methods For Selecting And Amplifying Polynucleotides
  • Optimization Of Multigene Analysis Of Tumor Samples
  • Partitioning Of Dna Sequencing Libraries Into Host And Microbial Components
  • Methods And Compositions For Genomic Target Enrichment And Selective Dna Sequencing
  • Targeted Screening For Mutations
  • Chromosome Conformation Capture Method Including Selection And Enrichment Steps
  • Materials And Methods For Determining Susceptibility Or Predisposition To Cancer
  • Fetal Genomic Analysis From A Maternal Biological Sample
  • Method And System For Rapid Searching Of Genomic Data And Uses Thereof
  • Methods For Selecting And Amplifying Polynucleotides
  • Method For The Simultaneous Amplification Of A Plurality Of Different Nucleic Acid Target Sequences
  • Detecting Mutations
  • Methods For Selecting And Amplifying Polynucleotides
  • Enrichment Of Dna Sequencing Libraries From Samples Containing Small Amounts Of Target Dna
  • Comprehensive In Vitro Reporting Of Cleavage Events By Sequencing (Circle-Seq)
  • Compositions And Methods Of Identifying Tumor Specific Neoantigens
  • Identification And Use Of Circulating Tumor Markers
  • Method And Systems For Enrichment Of Target Genomic Sequences
  • Methods And Systems For Detecting Genetic Mutations
  • Method And System For Rapid Searching Of Genomic Data And Uses Thereof
  • Optimization Of Multigene Analysis Of Tumor Samples
  • Molecular Coding For Analysis Of Composition Of Macromolecules And Molecular Complexes
  • Fetal Genomic Analysis From A Maternal Biological Sample
  • Screening For Structural Variants
  • High-Efficiency Hybrid Capture Compositions, And Methods
  • Mek1 Mutation Conferring Resistance To Raf And Mek Inhibitors
  • Pathogen Screening
  • Fetal Genomic Analysis From A Maternal Biological Sample
  • Multiplex Capture Of Nucleic Acids
  • Methods For Multi-Resolution Analysis Of Cell-Free Nucleic Acids
  • Compositions And Methods Of Identifying Tumor Specific Neoantigens
  • Individualized Vaccines For Cancer
  • Method Of Analyzing A Biological Sample From A Female Subject Pregnant With A Fetus
  • Analysis Methods
  • Genomewide Unbiased Identification Of Dsbs Evaluated By Sequencing (Guide-Seq)
  • Methods For Selecting And Amplifying Polynucleotides
  • Variant Database
  • Multiplex Capture Of Nucleic Acids
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.1523

    DOI

    http://dx.doi.org/10.1038/nbt.1523

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1040653661

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19182786


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Composition", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bayes Theorem", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biotinylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Exons", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genomics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nucleic Acid Hybridization", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oligonucleotide Array Sequence Analysis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oligonucleotides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymorphism, Single Nucleotide", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Reproducibility of Results", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sensitivity and Specificity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Analysis, DNA", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gnirke", 
            "givenName": "Andreas", 
            "id": "sg:person.0645200471.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645200471.91"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Melnikov", 
            "givenName": "Alexandre", 
            "id": "sg:person.01177153122.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177153122.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Maguire", 
            "givenName": "Jared", 
            "id": "sg:person.0633240146.72", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633240146.72"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rogov", 
            "givenName": "Peter", 
            "id": "sg:person.01352336663.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352336663.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Agilent Technologies (United States)", 
              "id": "https://www.grid.ac/institutes/grid.422638.9", 
              "name": [
                "Agilent Technologies Inc., 5301 Stevens Creek Blvd., Santa Clara, California 95051, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "LeProust", 
            "givenName": "Emily M", 
            "id": "sg:person.01320172170.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320172170.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brockman", 
            "givenName": "William", 
            "id": "sg:person.01010472411.59", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010472411.59"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fennell", 
            "givenName": "Timothy", 
            "id": "sg:person.016012720777.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016012720777.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Giannoukos", 
            "givenName": "Georgia", 
            "id": "sg:person.01241147411.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241147411.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fisher", 
            "givenName": "Sheila", 
            "id": "sg:person.01164364054.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164364054.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Russ", 
            "givenName": "Carsten", 
            "id": "sg:person.01300612454.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300612454.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gabriel", 
            "givenName": "Stacey", 
            "id": "sg:person.012151177437.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012151177437.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jaffe", 
            "givenName": "David B", 
            "id": "sg:person.01355660531.12", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355660531.12"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.", 
                "Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, Massachusetts 02139, USA.", 
                "Department of Systems Biology, Harvard Medical School, 200 Longwood Ave., Boston, Massachusetts 02115, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lander", 
            "givenName": "Eric S", 
            "id": "sg:person.01260666165.62", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260666165.62"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Broad Institute", 
              "id": "https://www.grid.ac/institutes/grid.66859.34", 
              "name": [
                "Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nusbaum", 
            "givenName": "Chad", 
            "id": "sg:person.01170225154.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170225154.35"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nbt821", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008728252", 
              "https://doi.org/10.1038/nbt821"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt821", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008728252", 
              "https://doi.org/10.1038/nbt821"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth1109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009647980", 
              "https://doi.org/10.1038/nmeth1109"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1117389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010697532"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng.2007.42", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012402265", 
              "https://doi.org/10.1038/ng.2007.42"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07484", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016969839", 
              "https://doi.org/10.1038/nature07484"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.828403", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018493357"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021189255", 
              "https://doi.org/10.1038/nmeth.1270"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03959", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021574562", 
              "https://doi.org/10.1038/nature03959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03959", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021574562", 
              "https://doi.org/10.1038/nature03959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth1111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025042325", 
              "https://doi.org/10.1038/nmeth1111"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0702165104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030762542"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07485", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034796960", 
              "https://doi.org/10.1038/nature07485"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkm078", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036425637"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.88.21.9623", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039166499"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.077776.108", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040737496"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0709013104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043093522"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gni070", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043825959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkn425", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044990606"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth1110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045927978", 
              "https://doi.org/10.1038/nmeth1110"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06884", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047672670", 
              "https://doi.org/10.1038/nature06884"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06258", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051134045", 
              "https://doi.org/10.1038/nature06258"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0803240105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051295955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth0105-63", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051721477", 
              "https://doi.org/10.1038/nmeth0105-63"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth0105-63", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051721477", 
              "https://doi.org/10.1038/nmeth0105-63"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07517", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052925719", 
              "https://doi.org/10.1038/nature07517"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.88.21.9628", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053104282"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.7522346", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062647427"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2009-02", 
        "datePublishedReg": "2009-02-01", 
        "description": "Targeting genomic loci by massively parallel sequencing requires new methods to enrich templates to be sequenced. We developed a capture method that uses biotinylated RNA 'baits' to fish targets out of a 'pond' of DNA fragments. The RNA is transcribed from PCR-amplified oligodeoxynucleotides originally synthesized on a microarray, generating sufficient bait for multiple captures at concentrations high enough to drive the hybridization. We tested this method with 170-mer baits that target >15,000 coding exons (2.5 Mb) and four regions (1.7 Mb total) using Illumina sequencing as read-out. About 90% of uniquely aligning bases fell on or near bait sequence; up to 50% lay on exons proper. The uniformity was such that approximately 60% of target bases in the exonic 'catch', and approximately 80% in the regional catch, had at least half the mean coverage. One lane of Illumina sequence was sufficient to call high-confidence genotypes for 89% of the targeted exon space.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt.1523", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2699326", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "27"
          }
        ], 
        "name": "Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing", 
        "pagination": "182-189", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "c1a874f93b47d2d5bd11e8802cfb853d663e10837bf0c931f53a1b9b92490943"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "19182786"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt.1523"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1040653661"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt.1523", 
          "https://app.dimensions.ai/details/publication/pub.1040653661"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T00:04", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8695_00000425.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nbt.1523"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt.1523'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt.1523'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt.1523'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt.1523'


     

    This table displays all metadata directly associated to this object as RDF triples.

    314 TRIPLES      21 PREDICATES      68 URIs      35 LITERALS      23 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt.1523 schema:about N02adcc9461d94b6384960ff0ccb076ea
    2 N1f608ce1b0c94df5be45ec69a7d1145c
    3 N34f5f792eeda489f829a742f8c9255c7
    4 N5223184f5ae14fba8d39741ea2744e44
    5 N68343c16a1d34f508c44442ca3248354
    6 N752619ee9e064433868b3c9627625aa8
    7 N774b312ac4494f2eb207f1e286a80a77
    8 N7d99bf1c6e4d4ddea220487f22cb3a79
    9 N87531deb37254c32995630574c7242ee
    10 Na94bcf4a1d6e4183bcfeba62bde3007c
    11 Nacfb2afaa6654159adebd93271b3673a
    12 Nb0d9e4d0d0f14a4991a51973632dbddb
    13 Ne000bba1e511433c9a6e6ad54c4935a9
    14 Nec3c70e0f5be4e1bbbce953959491c8c
    15 anzsrc-for:06
    16 anzsrc-for:0604
    17 schema:author N301fb78bcac24d4c8e088248e425e13d
    18 schema:citation sg:pub.10.1038/nature03959
    19 sg:pub.10.1038/nature06258
    20 sg:pub.10.1038/nature06884
    21 sg:pub.10.1038/nature07484
    22 sg:pub.10.1038/nature07485
    23 sg:pub.10.1038/nature07517
    24 sg:pub.10.1038/nbt821
    25 sg:pub.10.1038/ng.2007.42
    26 sg:pub.10.1038/nmeth.1270
    27 sg:pub.10.1038/nmeth0105-63
    28 sg:pub.10.1038/nmeth1109
    29 sg:pub.10.1038/nmeth1110
    30 sg:pub.10.1038/nmeth1111
    31 https://doi.org/10.1073/pnas.0702165104
    32 https://doi.org/10.1073/pnas.0709013104
    33 https://doi.org/10.1073/pnas.0803240105
    34 https://doi.org/10.1073/pnas.88.21.9623
    35 https://doi.org/10.1073/pnas.88.21.9628
    36 https://doi.org/10.1093/nar/gkm078
    37 https://doi.org/10.1093/nar/gkn425
    38 https://doi.org/10.1093/nar/gni070
    39 https://doi.org/10.1101/gr.077776.108
    40 https://doi.org/10.1101/gr.828403
    41 https://doi.org/10.1126/science.1117389
    42 https://doi.org/10.1126/science.7522346
    43 schema:datePublished 2009-02
    44 schema:datePublishedReg 2009-02-01
    45 schema:description Targeting genomic loci by massively parallel sequencing requires new methods to enrich templates to be sequenced. We developed a capture method that uses biotinylated RNA 'baits' to fish targets out of a 'pond' of DNA fragments. The RNA is transcribed from PCR-amplified oligodeoxynucleotides originally synthesized on a microarray, generating sufficient bait for multiple captures at concentrations high enough to drive the hybridization. We tested this method with 170-mer baits that target >15,000 coding exons (2.5 Mb) and four regions (1.7 Mb total) using Illumina sequencing as read-out. About 90% of uniquely aligning bases fell on or near bait sequence; up to 50% lay on exons proper. The uniformity was such that approximately 60% of target bases in the exonic 'catch', and approximately 80% in the regional catch, had at least half the mean coverage. One lane of Illumina sequence was sufficient to call high-confidence genotypes for 89% of the targeted exon space.
    46 schema:genre research_article
    47 schema:inLanguage en
    48 schema:isAccessibleForFree true
    49 schema:isPartOf N074ebd7cd60c420b9dc475aac8007cde
    50 N9c1d5f9069ff4fceac162481bb1eb49c
    51 sg:journal.1115214
    52 schema:name Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing
    53 schema:pagination 182-189
    54 schema:productId N28e3191f4ff34d1b90833d3d6f22fe19
    55 Nb1521a33b1a341fb9a5f10d9a4b0b782
    56 Nbace0e4e6e59457da42afd44ea68e35c
    57 Nc31e0b1d3b4e4b928764f61a247326b3
    58 Ne4e722ecaf4740589458a36a2c691190
    59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040653661
    60 https://doi.org/10.1038/nbt.1523
    61 schema:sdDatePublished 2019-04-11T00:04
    62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    63 schema:sdPublisher Nab8e2bfed902415d930b0eee2cf459e3
    64 schema:url http://www.nature.com/articles/nbt.1523
    65 sgo:license sg:explorer/license/
    66 sgo:sdDataset articles
    67 rdf:type schema:ScholarlyArticle
    68 N02adcc9461d94b6384960ff0ccb076ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    69 schema:name Genomics
    70 rdf:type schema:DefinedTerm
    71 N04535d102593438b9cfc2018fc84b762 rdf:first sg:person.01320172170.08
    72 rdf:rest N9adc139925984ff48cfd74f24de43df4
    73 N074ebd7cd60c420b9dc475aac8007cde schema:issueNumber 2
    74 rdf:type schema:PublicationIssue
    75 N0c08749b22d347bb971fb634b296f4c7 rdf:first sg:person.01170225154.35
    76 rdf:rest rdf:nil
    77 N0f1facfefaad434987a2206786b089fc rdf:first sg:person.01164364054.55
    78 rdf:rest Nb7eba17928db42c5b885f83bab3f88cc
    79 N1f608ce1b0c94df5be45ec69a7d1145c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Oligonucleotide Array Sequence Analysis
    81 rdf:type schema:DefinedTerm
    82 N201936cf72804868be76421772a20edc rdf:first sg:person.0633240146.72
    83 rdf:rest Nae1c997d9599460790c136c65b026794
    84 N28e3191f4ff34d1b90833d3d6f22fe19 schema:name nlm_unique_id
    85 schema:value 9604648
    86 rdf:type schema:PropertyValue
    87 N301fb78bcac24d4c8e088248e425e13d rdf:first sg:person.0645200471.91
    88 rdf:rest Na88286475e4f4447ab2786042ed55d18
    89 N34f5f792eeda489f829a742f8c9255c7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Sequence Analysis, DNA
    91 rdf:type schema:DefinedTerm
    92 N5223184f5ae14fba8d39741ea2744e44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Polymerase Chain Reaction
    94 rdf:type schema:DefinedTerm
    95 N6439b2152d45473a907a2cf9998a261b rdf:first sg:person.01355660531.12
    96 rdf:rest Nfd76cc82190543cf9e85baf31190b080
    97 N68343c16a1d34f508c44442ca3248354 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Reproducibility of Results
    99 rdf:type schema:DefinedTerm
    100 N6942fd0fc5ce48329f89b3cda44c46bd rdf:first sg:person.01241147411.31
    101 rdf:rest N0f1facfefaad434987a2206786b089fc
    102 N752619ee9e064433868b3c9627625aa8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Humans
    104 rdf:type schema:DefinedTerm
    105 N774b312ac4494f2eb207f1e286a80a77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    106 schema:name Oligonucleotides
    107 rdf:type schema:DefinedTerm
    108 N7d99bf1c6e4d4ddea220487f22cb3a79 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Sensitivity and Specificity
    110 rdf:type schema:DefinedTerm
    111 N87531deb37254c32995630574c7242ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Base Composition
    113 rdf:type schema:DefinedTerm
    114 N9adc139925984ff48cfd74f24de43df4 rdf:first sg:person.01010472411.59
    115 rdf:rest Ncf44cda6903449a98af468d273862bce
    116 N9c1d5f9069ff4fceac162481bb1eb49c schema:volumeNumber 27
    117 rdf:type schema:PublicationVolume
    118 Na88286475e4f4447ab2786042ed55d18 rdf:first sg:person.01177153122.09
    119 rdf:rest N201936cf72804868be76421772a20edc
    120 Na94bcf4a1d6e4183bcfeba62bde3007c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Nucleic Acid Hybridization
    122 rdf:type schema:DefinedTerm
    123 Naa8bf2c68e20443c9f923bd5a108cf8d rdf:first sg:person.012151177437.05
    124 rdf:rest N6439b2152d45473a907a2cf9998a261b
    125 Nab8e2bfed902415d930b0eee2cf459e3 schema:name Springer Nature - SN SciGraph project
    126 rdf:type schema:Organization
    127 Nacfb2afaa6654159adebd93271b3673a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Biotinylation
    129 rdf:type schema:DefinedTerm
    130 Nae1c997d9599460790c136c65b026794 rdf:first sg:person.01352336663.39
    131 rdf:rest N04535d102593438b9cfc2018fc84b762
    132 Nb0d9e4d0d0f14a4991a51973632dbddb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Exons
    134 rdf:type schema:DefinedTerm
    135 Nb1521a33b1a341fb9a5f10d9a4b0b782 schema:name dimensions_id
    136 schema:value pub.1040653661
    137 rdf:type schema:PropertyValue
    138 Nb7eba17928db42c5b885f83bab3f88cc rdf:first sg:person.01300612454.35
    139 rdf:rest Naa8bf2c68e20443c9f923bd5a108cf8d
    140 Nbace0e4e6e59457da42afd44ea68e35c schema:name doi
    141 schema:value 10.1038/nbt.1523
    142 rdf:type schema:PropertyValue
    143 Nc31e0b1d3b4e4b928764f61a247326b3 schema:name readcube_id
    144 schema:value c1a874f93b47d2d5bd11e8802cfb853d663e10837bf0c931f53a1b9b92490943
    145 rdf:type schema:PropertyValue
    146 Ncf44cda6903449a98af468d273862bce rdf:first sg:person.016012720777.47
    147 rdf:rest N6942fd0fc5ce48329f89b3cda44c46bd
    148 Ne000bba1e511433c9a6e6ad54c4935a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Bayes Theorem
    150 rdf:type schema:DefinedTerm
    151 Ne4e722ecaf4740589458a36a2c691190 schema:name pubmed_id
    152 schema:value 19182786
    153 rdf:type schema:PropertyValue
    154 Nec3c70e0f5be4e1bbbce953959491c8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Polymorphism, Single Nucleotide
    156 rdf:type schema:DefinedTerm
    157 Nfd76cc82190543cf9e85baf31190b080 rdf:first sg:person.01260666165.62
    158 rdf:rest N0c08749b22d347bb971fb634b296f4c7
    159 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    160 schema:name Biological Sciences
    161 rdf:type schema:DefinedTerm
    162 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    163 schema:name Genetics
    164 rdf:type schema:DefinedTerm
    165 sg:grant.2699326 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt.1523
    166 rdf:type schema:MonetaryGrant
    167 sg:journal.1115214 schema:issn 1087-0156
    168 1546-1696
    169 schema:name Nature Biotechnology
    170 rdf:type schema:Periodical
    171 sg:person.01010472411.59 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    172 schema:familyName Brockman
    173 schema:givenName William
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010472411.59
    175 rdf:type schema:Person
    176 sg:person.01164364054.55 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    177 schema:familyName Fisher
    178 schema:givenName Sheila
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164364054.55
    180 rdf:type schema:Person
    181 sg:person.01170225154.35 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    182 schema:familyName Nusbaum
    183 schema:givenName Chad
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170225154.35
    185 rdf:type schema:Person
    186 sg:person.01177153122.09 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    187 schema:familyName Melnikov
    188 schema:givenName Alexandre
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177153122.09
    190 rdf:type schema:Person
    191 sg:person.012151177437.05 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    192 schema:familyName Gabriel
    193 schema:givenName Stacey
    194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012151177437.05
    195 rdf:type schema:Person
    196 sg:person.01241147411.31 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    197 schema:familyName Giannoukos
    198 schema:givenName Georgia
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241147411.31
    200 rdf:type schema:Person
    201 sg:person.01260666165.62 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    202 schema:familyName Lander
    203 schema:givenName Eric S
    204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260666165.62
    205 rdf:type schema:Person
    206 sg:person.01300612454.35 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    207 schema:familyName Russ
    208 schema:givenName Carsten
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300612454.35
    210 rdf:type schema:Person
    211 sg:person.01320172170.08 schema:affiliation https://www.grid.ac/institutes/grid.422638.9
    212 schema:familyName LeProust
    213 schema:givenName Emily M
    214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320172170.08
    215 rdf:type schema:Person
    216 sg:person.01352336663.39 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    217 schema:familyName Rogov
    218 schema:givenName Peter
    219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352336663.39
    220 rdf:type schema:Person
    221 sg:person.01355660531.12 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    222 schema:familyName Jaffe
    223 schema:givenName David B
    224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355660531.12
    225 rdf:type schema:Person
    226 sg:person.016012720777.47 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    227 schema:familyName Fennell
    228 schema:givenName Timothy
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016012720777.47
    230 rdf:type schema:Person
    231 sg:person.0633240146.72 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    232 schema:familyName Maguire
    233 schema:givenName Jared
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633240146.72
    235 rdf:type schema:Person
    236 sg:person.0645200471.91 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
    237 schema:familyName Gnirke
    238 schema:givenName Andreas
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645200471.91
    240 rdf:type schema:Person
    241 sg:pub.10.1038/nature03959 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021574562
    242 https://doi.org/10.1038/nature03959
    243 rdf:type schema:CreativeWork
    244 sg:pub.10.1038/nature06258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051134045
    245 https://doi.org/10.1038/nature06258
    246 rdf:type schema:CreativeWork
    247 sg:pub.10.1038/nature06884 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047672670
    248 https://doi.org/10.1038/nature06884
    249 rdf:type schema:CreativeWork
    250 sg:pub.10.1038/nature07484 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016969839
    251 https://doi.org/10.1038/nature07484
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1038/nature07485 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034796960
    254 https://doi.org/10.1038/nature07485
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1038/nature07517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052925719
    257 https://doi.org/10.1038/nature07517
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1038/nbt821 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008728252
    260 https://doi.org/10.1038/nbt821
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1038/ng.2007.42 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012402265
    263 https://doi.org/10.1038/ng.2007.42
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1038/nmeth.1270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021189255
    266 https://doi.org/10.1038/nmeth.1270
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1038/nmeth0105-63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051721477
    269 https://doi.org/10.1038/nmeth0105-63
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1038/nmeth1109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009647980
    272 https://doi.org/10.1038/nmeth1109
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1038/nmeth1110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045927978
    275 https://doi.org/10.1038/nmeth1110
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1038/nmeth1111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025042325
    278 https://doi.org/10.1038/nmeth1111
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1073/pnas.0702165104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030762542
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1073/pnas.0709013104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043093522
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1073/pnas.0803240105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051295955
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1073/pnas.88.21.9623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039166499
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1073/pnas.88.21.9628 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053104282
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1093/nar/gkm078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036425637
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1093/nar/gkn425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044990606
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1093/nar/gni070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043825959
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1101/gr.077776.108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040737496
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1101/gr.828403 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018493357
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1126/science.1117389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010697532
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1126/science.7522346 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062647427
    303 rdf:type schema:CreativeWork
    304 https://www.grid.ac/institutes/grid.38142.3c schema:alternateName Harvard University
    305 schema:name Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.
    306 Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, Massachusetts 02139, USA.
    307 Department of Systems Biology, Harvard Medical School, 200 Longwood Ave., Boston, Massachusetts 02115, USA.
    308 rdf:type schema:Organization
    309 https://www.grid.ac/institutes/grid.422638.9 schema:alternateName Agilent Technologies (United States)
    310 schema:name Agilent Technologies Inc., 5301 Stevens Creek Blvd., Santa Clara, California 95051, USA.
    311 rdf:type schema:Organization
    312 https://www.grid.ac/institutes/grid.66859.34 schema:alternateName Broad Institute
    313 schema:name Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.
    314 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...