Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-02-01

AUTHORS

Andreas Gnirke, Alexandre Melnikov, Jared Maguire, Peter Rogov, Emily M LeProust, William Brockman, Timothy Fennell, Georgia Giannoukos, Sheila Fisher, Carsten Russ, Stacey Gabriel, David B Jaffe, Eric S Lander, Chad Nusbaum

ABSTRACT

Gnirke et al. present a bead-based method for capturing sequences of interest in the human genome for massively parallel sequencing. Using long, biotinylated RNA probes to pull down PCR-amplified DNA fragments, they demonstrate sequencing of 2.5 Mbs of exons in 1,900 genes.

PAGES

182-189

Journal

TITLE

Nature Biotechnology

ISSUE

2

VOLUME

27

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.1523

    DOI

    http://dx.doi.org/10.1038/nbt.1523

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1040653661

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19182786


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