Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-11

AUTHORS

Trond Aasen, Angel Raya, Maria J Barrero, Elena Garreta, Antonella Consiglio, Federico Gonzalez, Rita Vassena, Josipa Bilić, Vladimir Pekarik, Gustavo Tiscornia, Michael Edel, Stéphanie Boué, Juan Carlos Izpisúa Belmonte

ABSTRACT

The utility of induced pluripotent stem (iPS) cells for investigating the molecular logic of pluripotency and for eventual clinical application is limited by the low efficiency of current methods for reprogramming. Here we show that reprogramming of juvenile human primary keratinocytes by retroviral transduction with OCT4, SOX2, KLF4 and c-MYC is at least 100-fold more efficient and twofold faster compared with reprogramming of human fibroblasts. Keratinocyte-derived iPS (KiPS) cells appear indistinguishable from human embryonic stem cells in colony morphology, growth properties, expression of pluripotency-associated transcription factors and surface markers, global gene expression profiles and differentiation potential in vitro and in vivo. To underscore the efficiency and practicability of this technology, we generated KiPS cells from single adult human hairs. Our findings provide an experimental model for investigating the bases of cellular reprogramming and highlight potential advantages of using keratinocytes to generate patient-specific iPS cells. More... »

PAGES

1276-1284

Journal

TITLE

Nature Biotechnology

ISSUE

11

VOLUME

26

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.1503

    DOI

    http://dx.doi.org/10.1038/nbt.1503

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1026252022

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18931654


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